{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T21:16:27Z","timestamp":1793049387699,"version":"4.5.0"},"reference-count":45,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2023,2,20]],"date-time":"2023-02-20T00:00:00Z","timestamp":1676851200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000062","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01DK047320"],"award-info":[{"award-number":["R01DK047320"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000062","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["F32DK124963"],"award-info":[{"award-number":["F32DK124963"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000062","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01DK128390"],"award-info":[{"award-number":["R01DK128390"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000062","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["P20GM139753"],"award-info":[{"award-number":["P20GM139753"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Undergraduate Research Opportunities Program","award":["R01DK047320"],"award-info":[{"award-number":["R01DK047320"]}]},{"name":"Undergraduate Research Opportunities Program","award":["F32DK124963"],"award-info":[{"award-number":["F32DK124963"]}]},{"name":"Undergraduate Research Opportunities Program","award":["R01DK128390"],"award-info":[{"award-number":["R01DK128390"]}]},{"name":"Undergraduate Research Opportunities Program","award":["P20GM139753"],"award-info":[{"award-number":["P20GM139753"]}]},{"name":"Office of the Vice Provost for Research and Scholarship (OVPRS) at the University of Hawaii at Manoa","award":["R01DK047320"],"award-info":[{"award-number":["R01DK047320"]}]},{"name":"Office of the Vice Provost for Research and Scholarship (OVPRS) at the University of Hawaii at Manoa","award":["F32DK124963"],"award-info":[{"award-number":["F32DK124963"]}]},{"name":"Office of the Vice Provost for Research and Scholarship (OVPRS) at the University of Hawaii at Manoa","award":["R01DK128390"],"award-info":[{"award-number":["R01DK128390"]}]},{"name":"Office of the Vice Provost for Research and Scholarship (OVPRS) at the University of Hawaii at Manoa","award":["P20GM139753"],"award-info":[{"award-number":["P20GM139753"]}]},{"name":"Douglas S. Yamamura Scholarship","award":["R01DK047320"],"award-info":[{"award-number":["R01DK047320"]}]},{"name":"Douglas S. Yamamura Scholarship","award":["F32DK124963"],"award-info":[{"award-number":["F32DK124963"]}]},{"name":"Douglas S. Yamamura Scholarship","award":["R01DK128390"],"award-info":[{"award-number":["R01DK128390"]}]},{"name":"Douglas S. Yamamura Scholarship","award":["P20GM139753"],"award-info":[{"award-number":["P20GM139753"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>The use of glucocorticoid medications is known to cause metabolic side effects such as overeating, excess weight gain, and insulin resistance. The hypothalamus, a central regulator of feeding behavior and energy expenditure, is highly responsive to glucocorticoids, and it has been proposed that it plays a role in glucocorticoid-induced metabolic defects. Glucocorticoids can alter the expression and activity of antioxidant enzymes and promote the accumulation of reactive oxygen species. Recent evidence indicates that selenium can counter the effects of glucocorticoids, and selenium is critical for proper hypothalamic function. This study sought to determine whether selenium is capable of protecting hypothalamic cells from dysfunction caused by glucocorticoid exposure. We treated mHypoE-44 mouse hypothalamic cells with corticosterone to study the effects on cellular physiology and the involvement of selenium. We found that corticosterone administration rendered cells more vulnerable to endoplasmic reticulum stress and the subsequent impairment of insulin signaling. Supplementing the cell culture media with additional selenium alleviated endoplasmic reticulum stress and promoted insulin signaling. These findings implicate a protective role of selenium against chronic glucocorticoid-induced hypothalamic dysfunction.<\/jats:p>","DOI":"10.3390\/antiox12020526","type":"journal-article","created":{"date-parts":[[2023,2,20]],"date-time":"2023-02-20T04:58:23Z","timestamp":1676869103000},"page":"526","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Selenium Protects Mouse Hypothalamic Cells from Glucocorticoid-Induced Endoplasmic Reticulum Stress Vulnerability and Insulin Signaling Impairment"],"prefix":"10.3390","volume":"12","author":[{"given":"Katlyn J.","family":"An","sequence":"first","affiliation":[{"name":"Department of Cell and Molecular Biology, John A. Burns School of Medicine, University of Hawai\u2018i, Honolulu, HI 96813, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashley N.","family":"Hanato","sequence":"additional","affiliation":[{"name":"Department of Cell and Molecular Biology, John A. Burns School of Medicine, University of Hawai\u2018i, Honolulu, HI 96813, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katherine W.","family":"Hui","sequence":"additional","affiliation":[{"name":"Department of Cell and Molecular Biology, John A. Burns School of Medicine, University of Hawai\u2018i, Honolulu, HI 96813, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew W.","family":"Pitts","sequence":"additional","affiliation":[{"name":"Department of Cell and Molecular Biology, John A. Burns School of Medicine, University of Hawai\u2018i, Honolulu, HI 96813, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0686-7516","authenticated-orcid":false,"given":"Lucia A.","family":"Seale","sequence":"additional","affiliation":[{"name":"Pacific Biosciences Research Center, School of Ocean and Earth Science and Technology, University of Hawai\u2018i, Honolulu, HI 96822, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica L.","family":"Nicholson","sequence":"additional","affiliation":[{"name":"Department of Cell and Molecular Biology, John A. Burns School of Medicine, University of Hawai\u2018i, Honolulu, HI 96813, USA"},{"name":"Pacific Biosciences Research Center, School of Ocean and Earth Science and Technology, University of Hawai\u2018i, Honolulu, HI 96822, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pamela","family":"Toh","sequence":"additional","affiliation":[{"name":"Pacific Biosciences Research Center, School of Ocean and Earth Science and Technology, University of Hawai\u2018i, Honolulu, HI 96822, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun Kyoung","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Cell and Molecular Biology, John A. Burns School of Medicine, University of Hawai\u2018i, Honolulu, HI 96813, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2428-586X","authenticated-orcid":false,"given":"Marla J.","family":"Berry","sequence":"additional","affiliation":[{"name":"Pacific Biosciences Research Center, School of Ocean and Earth Science and Technology, University of Hawai\u2018i, Honolulu, HI 96822, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4451-712X","authenticated-orcid":false,"given":"Daniel J.","family":"Torres","sequence":"additional","affiliation":[{"name":"Department of Cell and Molecular Biology, John A. Burns School of Medicine, University of Hawai\u2018i, Honolulu, HI 96813, USA"},{"name":"Pacific Biosciences Research Center, School of Ocean and Earth Science and Technology, University of Hawai\u2018i, Honolulu, HI 96822, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/B978-0-444-64123-6.00004-7","article-title":"Hypothalamic-pituitary-adrenal axis and stress","volume":"175","author":"Leistner","year":"2020","journal-title":"Handb. Clin. Neurol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1002\/acr.21796","article-title":"Prevalence of oral glucocorticoid usage in the United States: A general population perspective","volume":"65","author":"Overman","year":"2013","journal-title":"Arthritis Care Res. 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