{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T10:42:18Z","timestamp":1775558538271,"version":"3.50.1"},"reference-count":63,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2023,3,6]],"date-time":"2023-03-06T00:00:00Z","timestamp":1678060800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>In this study, we shed light for the first time on the usage of migratory locusts (Locusta migratoria) as an insect model to investigate the nanotoxicological influence of aluminum oxide (Al2O3) nanoparticles at low doses on testes, and evaluate the capacity of a whole-body extract of American cockroaches (Periplaneta americana) (PAE) to attenuate Al2O3 NPs-induced toxicity. Energy dispersive X-ray microanalyzer (EDX) analysis verified the bioaccumulation of Al in testicular tissues due to its liberation from Al2O3 NPs, implying their penetration into the blood\u2013testis barrier. Remarkably, toxicity with Al engendered disorders of antioxidant and stress biomarkers associated with substantial DNA damage and cell apoptosis. Furthermore, histopathological and ultrastructural analyses manifested significant aberrations in the testicular tissues from the group exposed to Al2O3 NPs, indicating the overproduction of reactive oxygen species (ROS). Molecular docking analysis emphasized the antioxidant capacity of some compounds derived from PAE. Thus, pretreatment with PAE counteracted the detrimental effects of Al in the testes, revealing antioxidant properties and thwarting DNA impairment and cell apoptosis. Moreover, histological and ultrastructural examinations revealed no anomalies in the testes. Overall, these findings substantiate the potential applications of PAE in preventing the testicular impairment of L. migratoria and the conceivable utilization of locusts for nanotoxicology studies.<\/jats:p>","DOI":"10.3390\/antiox12030653","type":"journal-article","created":{"date-parts":[[2023,3,6]],"date-time":"2023-03-06T05:18:43Z","timestamp":1678079923000},"page":"653","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Entomotherapeutic Role of Periplaneta americana Extract in Alleviating Aluminum Oxide Nanoparticles-Induced Testicular Oxidative Impairment in Migratory Locusts (Locusta migratoria) as an Ecotoxicological Model"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7908-8863","authenticated-orcid":false,"given":"Esraa A.","family":"Arafat","sequence":"first","affiliation":[{"name":"Department of Zoology, Faculty of Science, Alexandria University, Alexandria 21321, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2366-418X","authenticated-orcid":false,"given":"Doaa S.","family":"El-Sayed","sequence":"additional","affiliation":[{"name":"Chemistry Department, Faculty of Science, Alexandria University, Alexandria 21321, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hussein K.","family":"Hussein","sequence":"additional","affiliation":[{"name":"Department of Zoology, Faculty of Science, Alexandria University, Alexandria 21321, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Justin","family":"Flaven-Pouchon","sequence":"additional","affiliation":[{"name":"Interfaculty Institute for Cell Biology, Eberhard-Karls Universit\u00e4t T\u00fcbingen, 37073 T\u00fcbingen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernard","family":"Moussian","sequence":"additional","affiliation":[{"name":"Universit\u00e9 C\u00f4te d\u2019Azur, Parc Valrose, 06000 Nice, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4352-5742","authenticated-orcid":false,"given":"Lamia M.","family":"El-Samad","sequence":"additional","affiliation":[{"name":"Department of Zoology, Faculty of Science, Alexandria University, Alexandria 21321, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2117-0677","authenticated-orcid":false,"given":"Abeer","family":"El Wakil","sequence":"additional","affiliation":[{"name":"Department of Biological and Geological Sciences, Faculty of Education, Alexandria University, Alexandria 21526, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2620-7598","authenticated-orcid":false,"given":"Mohamed A.","family":"Hassan","sequence":"additional","affiliation":[{"name":"Protein Research Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City 21934, Egypt"},{"name":"University Medical Center G\u00f6ttingen, Georg-August-University, 37073 G\u00f6ttingen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1016\/j.ejpb.2013.01.001","article-title":"Nanotoxicological classification system (NCS)\u2014A guide for the risk-benefit assessment of nanoparticulate drug delivery systems","volume":"84","author":"Keck","year":"2013","journal-title":"Eur. 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