{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T02:40:23Z","timestamp":1771296023262,"version":"3.50.1"},"reference-count":71,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2023,3,12]],"date-time":"2023-03-12T00:00:00Z","timestamp":1678579200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Central Public-interest Scientific Institution Basal Research Fund","award":["2021XT08"],"award-info":[{"award-number":["2021XT08"]}]},{"name":"Central Public-interest Scientific Institution Basal Research Fund","award":["2020TD3"],"award-info":[{"award-number":["2020TD3"]}]},{"name":"Central Public-interest Scientific Institution Basal Research Fund","award":["32002363"],"award-info":[{"award-number":["32002363"]}]},{"name":"National Natural Science Foundation of China","award":["2021XT08"],"award-info":[{"award-number":["2021XT08"]}]},{"name":"National Natural Science Foundation of China","award":["2020TD3"],"award-info":[{"award-number":["2020TD3"]}]},{"name":"National Natural Science Foundation of China","award":["32002363"],"award-info":[{"award-number":["32002363"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>The enzyme 1-acylglycerol-3-phosphate O-acyltransferase 2 (AGPAT2) is an intermediate enzyme in triglyceride synthesis. The aim was to study the regulatory mechanism of AGPAT2 on Nile tilapia, Oreochromis niloticus. In this study, antisense RNA technology was used to knock-down AGPAT2 in Nile tilapia. Compared with the control groups (transfected with ultrapure water or the blank expression vector), the AGPAT2 knock-down group showed a significantly higher weight gain rate, special growth rate, visceral somatic index, and hepatopancreas somatic index; and significantly increased the total cholesterol, triglycerides, glucose, low-density lipoprotein cholesterol, and insulin levels in serum. In addition, the contents of total cholesterol and triglycerides and the abundance of superoxide dismutase, catalase, and glutathione peroxidase in the liver significantly increased, while the malondialdehyde content significantly decreased. The liver cells became severely vacuolated and accumulated lipids in the AGPAT2 knock-down group. Comparative transcriptome analyses (AGPAT2 knock-down vs. control group) revealed 1789 differentially expressed genes (DEGs), including 472 upregulated genes and 1313 downregulated genes in the AGPAT2 knock-down group. Functional analysis showed that the main pathway of differentially expressed genes enrichment was lipid metabolism and oxidative stress, such as steroid biosynthesis, unsaturated fatty acid biosynthesis, the PPAR signaling pathway, and the P53 pathway. We used qRT-PCR to verify the mRNA expression changes of 13 downstream differential genes in related signaling pathways. These findings demonstrate that knock-down of AGPAT2 in tilapia leads to abnormal lipid metabolism and oxidative stress.<\/jats:p>","DOI":"10.3390\/antiox12030700","type":"journal-article","created":{"date-parts":[[2023,3,13]],"date-time":"2023-03-13T06:09:56Z","timestamp":1678687796000},"page":"700","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Transcriptional Inhibition of AGPAT2 Induces Abnormal Lipid Metabolism and Oxidative Stress in the Liver of Nile Tilapia Oreochromis niloticus"],"prefix":"10.3390","volume":"12","author":[{"given":"Tiantian","family":"Feng","sequence":"first","affiliation":[{"name":"School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing 210023, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yifan","family":"Tao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Freshwater Fisheries Research Center, Ministry of Agriculture and Rural Affairs, Chinese Academy of Fishery Sciences, Wuxi 214081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yue","family":"Yan","sequence":"additional","affiliation":[{"name":"Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Siqi","family":"Lu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Freshwater Fisheries Research Center, Ministry of Agriculture and Rural Affairs, Chinese Academy of Fishery Sciences, Wuxi 214081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Freshwater Fisheries Research Center, Ministry of Agriculture and Rural Affairs, Chinese Academy of Fishery Sciences, Wuxi 214081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xing","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing 210023, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Qiang","sequence":"additional","affiliation":[{"name":"School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing 210023, China"},{"name":"Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Freshwater Fisheries Research Center, Ministry of Agriculture and Rural Affairs, Chinese Academy of Fishery Sciences, Wuxi 214081, China"},{"name":"Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,12]]},"reference":[{"key":"ref_1","first-page":"539","article-title":"Effect of Different Salinity Level on Breeding, Fertilization, Hatching and Survival of Nile Tilapia, Oreochromis niloticus (Linnaeus, 1758) in Captivity","volume":"50","author":"Malik","year":"2018","journal-title":"Pak. J. Zool."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1016\/j.aquaculture.2003.11.010","article-title":"Regulation of feed intake, growth, nutrient and energy utilisation in European sea bass (Dicentrarchus labrax) fed high fat diets","volume":"231","author":"Boujard","year":"2004","journal-title":"Aquaculture"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.dci.2019.03.009","article-title":"Chronic inflammation is a key to inducing liver injury in blunt snout bream (Megalobrama amblycephala) fed with high-fat diet","volume":"97","author":"Dai","year":"2019","journal-title":"Dev. Comp. Immunol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1097\/MOL.0b013e328354fcf4","article-title":"Lysophospholipid acyltransferases: 1-acylglycerol-3-phosphate O-acyltransferases. From discovery to disease","volume":"23","author":"Agarwal","year":"2012","journal-title":"Curr. Opin. Lipidol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1097\/MOL.0000000000000492","article-title":"The lysophosphatidic acid acyltransferases (acylglycerophosphate acyltransferases) family: One reaction, five enzymes, many roles","volume":"29","author":"Bradley","year":"2018","journal-title":"Curr. Opin. Lipidol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1038\/ng880","article-title":"AGPAT2 is mutated in congenital generalized lipodystrophy linked to chromosome 9q34","volume":"31","author":"Agarwal","year":"2002","journal-title":"Nat. Genet."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"446","DOI":"10.1016\/j.bbrc.2004.12.024","article-title":"Enzymatic activity of naturally occurring 1-acylglycerol-3-phosphate-O-acyltransferase 2 mutants associated with congenital generalized lipodystrophy","volume":"327","author":"Haque","year":"2005","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"63","DOI":"10.3389\/fcell.2020.00063","article-title":"CDP-Diacylglycerol Synthases (CDS): Gateway to Phosphatidylinositol and Cardiolipin Synthesis","volume":"8","author":"Blunsom","year":"2020","journal-title":"Front. Cell Dev. Biol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"11082","DOI":"10.1074\/jbc.M509612200","article-title":"A regulatory role for 1-acylglycerol-3-phosphate-O-acyltransferase 2 in adipocyte differentiation","volume":"281","author":"Gale","year":"2006","journal-title":"J. Biol. Chem."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/j.cmet.2009.01.002","article-title":"Molecular Mechanisms of Hepatic Steatosis and Insulin Resistance in the AGPAT2-Deficient Mouse Model of Congenital Generalized Lipodystrophy","volume":"9","author":"Cortes","year":"2009","journal-title":"Cell Metab."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"6877","DOI":"10.1038\/s41467-021-27279-4","article-title":"AGPAT2 interaction with CDP-diacylglycerol synthases promotes the flux of fatty acids through the CDP-diacylglycerol pathway","volume":"12","author":"Mak","year":"2021","journal-title":"Nat. Commun."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1517\/14728222.7.5.643","article-title":"Lysophosphatidic acid acyltransferase-\u03b2: A novel target for induction of tumour cell apoptosis","volume":"7","author":"Bonham","year":"2003","journal-title":"Expert Opin. Ther. Targets"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1016\/j.cbpb.2010.08.010","article-title":"Effects of lysophosphatidic acid on the in vitro proliferation and differentiation of a novel porcine preadipocyte cell line","volume":"157","author":"Nobusue","year":"2010","journal-title":"Comp. Biochem. Physiol. Part B Biochem. Mol. Biol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"927","DOI":"10.1016\/j.metabol.2009.10.012","article-title":"Palmitate induces insulin resistance without significant intracellular triglyceride accumulation in HepG2 cells","volume":"59","author":"Lee","year":"2010","journal-title":"Metab. Clin. Exp."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1007\/s10620-013-2896-2","article-title":"Saturated Free Fatty Acid Sodium Palmitate-Induced Lipoapoptosis by Targeting Glycogen Synthase Kinase-3\u03b2 Activation in Human Liver Cells","volume":"59","author":"Cao","year":"2014","journal-title":"Dig. Dis. Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"146203","DOI":"10.1016\/j.gene.2021.146023","article-title":"Transcriptional inhibition of steroidogenic factor 1 in vivo in Oreochromis niloticus increased weight and suppressed gonad development","volume":"809","author":"Cao","year":"2022","journal-title":"Gene"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1111\/j.1749-7345.2008.00167.x","article-title":"Gene knockdown: A powerful tool for gene function study in fish","volume":"39","author":"Boonanuntanasarn","year":"2008","journal-title":"J. World Aquac. Soc."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1421","DOI":"10.1073\/pnas.78.3.1421","article-title":"Inhibition of ColE1 RNA primer formation by a plasmid-specified small RNA","volume":"78","author":"Tomizawa","year":"1981","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1001","DOI":"10.1006\/bbrc.1994.1801","article-title":"Antisense src expression inhibits U937 human leukemia cell proliferation in conjunction with reduction of c-myb expression","volume":"201","author":"Waki","year":"1994","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1016\/0014-5793(96)00773-9","article-title":"Reduction of 14-16 kDa allergenic proteins in transgenic rice plants by antisense gene","volume":"391","author":"Tada","year":"1996","journal-title":"FEBS Lett."},{"key":"ref_21","first-page":"118","article-title":"Downregulation of lanosterol synthase gene expression by antisense RNA technology in Saccharomyces cerevisiae","volume":"50","author":"Wang","year":"2015","journal-title":"Acta Pharm. Sin."},{"key":"ref_22","first-page":"146792","article-title":"Knock-down of amh transcription by antisense RNA reduces FSH and increases follicular atresia in female","volume":"842","author":"Qiang","year":"2022","journal-title":"Oreochromis Niloticus Gene"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Yan, Y., Tao, Y.F., Cao, Z.M., Lu, S.Q., Xu, P., and Qiang, J. (2022). The Effect of Knocked-Down Anti-Mullerian Hormone mRNA on Reproductive Characters of Male Nile Tilapia (Oreochromis niloticus) through Inhibition of the TGF-Beta Signaling Pathway. Fishes, 7.","DOI":"10.3390\/fishes7050299"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1016\/S0021-9258(18)64849-5","article-title":"A simple method for the isolation and purification of total lipides from animal tissues","volume":"226","author":"Folch","year":"1957","journal-title":"J. Biol. Chem."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1521","DOI":"10.3389\/fphys.2018.01521","article-title":"Changes in Physiological Parameters, Lipid Metabolism, and Expression of MicroRNAs in Genetically Improved Farmed Tilapia (Oreochromis niloticus) with Fatty Liver Induced by a High-Fat Diet","volume":"9","author":"Tao","year":"2018","journal-title":"Front. Physiol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1038\/nbt.1621","article-title":"Transcript assembly and quantification by RNA-Seq reveals unannotated transcripts and isoform switching during cell differentiation","volume":"28","author":"Trapnell","year":"2010","journal-title":"Nat. Biotechnol."},{"key":"ref_27","first-page":"100784","article-title":"Transcriptome profiling reveal Acanthopanax senticosus improves growth performance, immunity and antioxidant capacity by regulating lipid metabolism in GIFT (Oreochromis niloticus)","volume":"37","author":"Li","year":"2021","journal-title":"Comp. Biochem. Physiol. Part D Genom. Proteom."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2\u2212\u0394\u0394CT method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1422","DOI":"10.3389\/fphys.2018.01422","article-title":"High Fat Diet-Induced miR-122 Regulates Lipid Metabolism and Fat Deposition in Genetically Improved Farmed Tilapia (GIFT, Oreochromis niloticus) Liver","volume":"9","author":"Qiang","year":"2018","journal-title":"Front. Physiol."},{"key":"ref_30","first-page":"1628","article-title":"Causes of fatty liver in farmed fish: A review and new perspectives","volume":"38","author":"Du","year":"2014","journal-title":"J. Fish. China"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"812","DOI":"10.1002\/hep.30538","article-title":"Association Between Nonalcoholic Fatty Liver Disease and Reduced Bone Mineral Density in Children: A Meta-Analysis","volume":"70","author":"Mantovani","year":"2019","journal-title":"Hepatology"},{"key":"ref_32","first-page":"1165","article-title":"Research progress of the mechanism and therapy of non-alcoholic fatty liver disease","volume":"30","author":"Guo","year":"2018","journal-title":"Chin. Bull. Life Sci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"990193","DOI":"10.3389\/fmars.2022.990193","article-title":"Lipid metabolism disorders of hybrid grouper (\u2640Epinephelus fuscointestinestatus x male \u2642E. lanceolatu) induced by high-lipid diet","volume":"9","author":"Suo","year":"2022","journal-title":"Front. Mar. Sci."},{"key":"ref_34","first-page":"3077","article-title":"Postmortem findings in congenital generalized lipodystrophy","volume":"80","author":"Chandalia","year":"1995","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"8927","DOI":"10.1038\/s41598-020-65535-7","article-title":"Liver colour scoring index, carotenoids and lipid content assessment as a proxy for lumpfish (Cyclopterus lumpus L.) health and welfare condition","volume":"10","author":"Eliasen","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"3182","DOI":"10.1007\/s10620-019-05657-9","article-title":"Vitamin E Ameliorates Lipid Metabolism in Mice with Nonalcoholic Fatty Liver Disease via Nrf2\/CES1 Signaling Pathway","volume":"64","author":"He","year":"2019","journal-title":"Dig. Dis. Sci."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1016\/j.aquaculture.2013.06.003","article-title":"Hepatic triacylglycerol secretion, lipid transport and tissue lipid uptake in blunt snout bream (Megalobrama amblycephala) fed high-fat diet","volume":"408","author":"Lu","year":"2013","journal-title":"Aquaculture"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"2567","DOI":"10.1242\/jeb.070581","article-title":"Regulation of metabolism by dietary carbohydrates in two lines of rainbow trout divergently selected for muscle fat content","volume":"215","author":"Kamalam","year":"2012","journal-title":"J. Exp. Biol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"392","DOI":"10.1002\/naaq.10250","article-title":"Dietary Sodium Butyrate Supplementation Attenuates the Detrimental Effects of High-Fat Diets on Growth Performance, Liver Health, and Disease Resistance in Grass Carp","volume":"84","author":"Gao","year":"2022","journal-title":"N. Am. J. Aquac."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1007\/s10695-016-0268-5","article-title":"Berberine attenuates oxidative stress and hepatocytes apoptosis via protecting mitochondria in blunt snout bream Megalobrama amblycephala fed high-fat diets","volume":"43","author":"Lu","year":"2017","journal-title":"Fish Physiol. Biochem."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1016\/S0044-8486(03)00025-5","article-title":"Fish performance, fillet characteristics, and health assessment index of rainbow trout (Oncorhynchus mykiss) fed diets containing adequate and high concentrations of lipid and vitamin E","volume":"219","author":"Chaiyapechara","year":"2003","journal-title":"Aquaculture"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1016\/j.foodchem.2015.12.003","article-title":"Enhanced muscle nutrient content and flesh quality, resulting from tryptophan, is associated with anti-oxidative damage referred to the Nrf2 and TOR signalling factors in young grass carp (Ctenopharyngodon idella): Avoid tryptophan deficiency or excess","volume":"199","author":"Jiang","year":"2016","journal-title":"Food Chem."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"894","DOI":"10.1016\/j.fsi.2018.10.084","article-title":"Antioxidative, inflammatory and immune responses in hydrogen peroxide-induced liver injury of tilapia (GIFT, Oreochromis niloticus)","volume":"84","author":"Jia","year":"2019","journal-title":"Fish Shellfish Immunol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.taap.2009.11.028","article-title":"Oxidative stress and oxidative damage in chemical carcinogenesis","volume":"254","author":"Klaunig","year":"2011","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1161\/01.STR.32.3.741","article-title":"Overexpression of copper-zinc superoxide dismutase attenuates acute activation of activator protein-1 after transient focal cerebral ischemia in mice","volume":"32","author":"Huang","year":"2001","journal-title":"Stroke"},{"key":"ref_46","first-page":"276","article-title":"PPARs: Regulators of metabolism and as therapeutic targets in cardiovascular disease. Part II: PPAR-ss\/delta and PPAR-gamma","volume":"13","author":"Han","year":"2017","journal-title":"Future Cardiol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1385\/CBB:32:1-3:187","article-title":"Peroxisome proliferator-activated receptors, coactivators, and downstream targets","volume":"32","author":"Qi","year":"2000","journal-title":"Cell Biochem. Biophys."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"809","DOI":"10.1016\/j.bbalip.2011.10.016","article-title":"Roles of PPARs in NAFLD: Potential therapeutic targets","volume":"1821","author":"Tailleux","year":"2012","journal-title":"Biochim. Biophys. Acta Mol. Cell Biol. Lipids"},{"key":"ref_49","first-page":"144","article-title":"Advances in the structure-activity relationships of chlorogenic acid derivatives","volume":"28","author":"Yu","year":"2018","journal-title":"Chin. J. Med. Chem."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Yun, J.Y., Jin, H.G., Cao, Y., Zhang, L.C., Zhao, Y.M., Jin, X., and Yu, Y.S. (2018). RNA-Seq Analysis Reveals a Positive Role of HTR2A in Adipogenesis in Yan Yellow Cattle. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19061760"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1229","DOI":"10.1387\/ijdb.092976rl","article-title":"Fatty acid binding proteins in brain development and disease","volume":"54","author":"Liu","year":"2010","journal-title":"Int. J. Dev. Biol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"168","DOI":"10.2174\/138161211795049615","article-title":"Role of Polyunsaturated Fatty Acids and Fatty Acid Binding Protein in the Pathogenesis of Schizophrenia","volume":"17","author":"Maekawa","year":"2011","journal-title":"Curr. Pharm. Des."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/j.gene.2015.05.027","article-title":"Molecular cloning and expression analysis of scd1 gene from large yellow croaker Larimichthys crocea under cold stress","volume":"568","author":"Xu","year":"2015","journal-title":"Gene"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"30132","DOI":"10.1074\/jbc.M005488200","article-title":"The biosynthesis of hepatic cholesterol esters and triglycerides is impaired in mice with a disruption of the gene for stearoyl-CoA desaturase 1","volume":"275","author":"Miyazaki","year":"2000","journal-title":"J. Biol. Chem."},{"key":"ref_55","first-page":"63","article-title":"Influence of Low Temperature and Feeding on Expressions of Unsaturated Fatty Acid Biosynthesis Genes of Rainbow Trout (Oncorhynchus mykiss)","volume":"49","author":"Liu","year":"2019","journal-title":"Period. Ocean Univ. China"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.aggene.2017.09.006","article-title":"Genes, transcription factors and enzymes involved in lipid metabolism in fin fish","volume":"7","author":"Ayisi","year":"2018","journal-title":"Agri Gene"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"4104","DOI":"10.3748\/wjg.v24.i36.4104","article-title":"Role of microRNAs in alcohol-induced liver disorders and non-alcoholic fatty liver disease","volume":"24","author":"Torres","year":"2018","journal-title":"World J. Gastroenterol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1194\/jlr.M087890","article-title":"Lysophospholipases cooperate to mediate lipid homeostasis and lysophospholipid signaling","volume":"60","author":"Wepy","year":"2019","journal-title":"J. Lipid Res."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1016\/j.bbalip.2008.10.010","article-title":"Glycerol-3-phosphate acyltransferases: Rate limiting enzymes of triacylglycerol biosynthesis","volume":"1791","author":"Wendel","year":"2009","journal-title":"Biochim. Biophys. Acta Mol. Cell Biol. Lipids"},{"key":"ref_60","first-page":"1167","article-title":"Progress in the study on mammalian diacylgycerol acyltransgerase (DGAT) gene and its biological function","volume":"29","author":"Wang","year":"2007","journal-title":"Hereditas"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1016\/j.bbrc.2017.03.170","article-title":"Atgl gene deletion predisposes to proximal tubule damage by impairing the fatty acid metabolism","volume":"487","author":"Chen","year":"2017","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"1069","DOI":"10.1194\/jlr.M090928","article-title":"PNPLA2 influences secretion of triglyceride-rich lipoproteins by human hepatoma cells","volume":"60","author":"Taxiarchis","year":"2019","journal-title":"J. Lipid Res."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"17877","DOI":"10.1074\/jbc.REV120.013529","article-title":"Genetic diseases of the Kennedy pathways for membrane synthesis","volume":"295","author":"Tavasoli","year":"2020","journal-title":"J. Biol. Chem."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1159\/000322326","article-title":"60 kDa Lysophospholipase, a New Sgk1 Molecular Partner Involved in the Regulation of EnaC","volume":"26","author":"Menniti","year":"2010","journal-title":"Cell. Physiol. Biochem."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1007\/s10545-014-9748-x","article-title":"Lipid metabolism in mitochondrial membranes","volume":"38","author":"Mayr","year":"2015","journal-title":"J. Inherit. Metab. Dis."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.cbpa.2016.09.001","article-title":"Molecular drivers of mitochondrial membrane proliferation in response to cold acclimation in threespine stickleback","volume":"203","author":"Keenan","year":"2017","journal-title":"Comp. Biochem. Physiol. A Mol. Integr. Physiol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1016\/j.jembe.2006.05.013","article-title":"Antioxidant responses and lipid peroxidation in gills and erythrocytes of fish (Rhabdosarga sarba) upon exposure to Chattonella marina and hydrogen peroxide: Implications on the cause of fish kills","volume":"336","author":"Woo","year":"2006","journal-title":"J. Exp. Mar. Biol. Ecol."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"154341","DOI":"10.1016\/j.metabol.2020.154341","article-title":"Absence of AGPAT2 impairs brown adipogenesis, increases IFN stimulated gene expression and alters mitochondrial morphology","volume":"111","author":"Tapia","year":"2020","journal-title":"Metab. Clin. Exp."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"1293","DOI":"10.1002\/(SICI)1097-0142(19990315)85:6<1293::AID-CNCR11>3.0.CO;2-O","article-title":"p53 protein overexpression is associated with increased cell proliferation in patients with locally recurrent prostate carcinoma after radiation therapy","volume":"85","author":"Cheng","year":"1999","journal-title":"Cancer"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"1013","DOI":"10.1016\/0092-8674(94)90379-4","article-title":"p53-dependent inhibition of cyclin-dependent kinase activities in human fibroblasts during radiation-induced G1 arrest","volume":"76","author":"Dulic","year":"1994","journal-title":"Cell"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1002\/hep.26683","article-title":"Hepatic Oxidative Stress Activates the Gadd45b Gene by Way of Degradation of the Transcriptional Repressor STAT3","volume":"59","author":"Kim","year":"2014","journal-title":"Hepatology"}],"container-title":["Antioxidants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-3921\/12\/3\/700\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T18:53:03Z","timestamp":1760122383000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-3921\/12\/3\/700"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3,12]]},"references-count":71,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2023,3]]}},"alternative-id":["antiox12030700"],"URL":"https:\/\/doi.org\/10.3390\/antiox12030700","relation":{},"ISSN":["2076-3921"],"issn-type":[{"value":"2076-3921","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,3,12]]}}}