{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T05:30:39Z","timestamp":1783488639399,"version":"3.55.0"},"reference-count":82,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2023,4,7]],"date-time":"2023-04-07T00:00:00Z","timestamp":1680825600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Key R&amp;D Program of China","award":["2022YFE0100200"],"award-info":[{"award-number":["2022YFE0100200"]}]},{"name":"National Key R&amp;D Program of China","award":["32161143032"],"award-info":[{"award-number":["32161143032"]}]},{"name":"National Key R&amp;D Program of China","award":["31972568"],"award-info":[{"award-number":["31972568"]}]},{"name":"National Key R&amp;D Program of China","award":["6202027"],"award-info":[{"award-number":["6202027"]}]},{"name":"National Key R&amp;D Program of China","award":["CARS36"],"award-info":[{"award-number":["CARS36"]}]},{"name":"National Key R&amp;D Program of China","award":["ASTIP-2016-IAS-06"],"award-info":[{"award-number":["ASTIP-2016-IAS-06"]}]},{"name":"National Key R&amp;D Program of China","award":["21626604D"],"award-info":[{"award-number":["21626604D"]}]},{"name":"National Key R&amp;D Program of China","award":["21326608D"],"award-info":[{"award-number":["21326608D"]}]},{"name":"National Key R&amp;D Program of China","award":["G2022003015L"],"award-info":[{"award-number":["G2022003015L"]}]},{"name":"National Natural Science Foundation of China","award":["2022YFE0100200"],"award-info":[{"award-number":["2022YFE0100200"]}]},{"name":"National Natural Science Foundation of China","award":["32161143032"],"award-info":[{"award-number":["32161143032"]}]},{"name":"National Natural Science Foundation of China","award":["31972568"],"award-info":[{"award-number":["31972568"]}]},{"name":"National Natural Science Foundation of China","award":["6202027"],"award-info":[{"award-number":["6202027"]}]},{"name":"National Natural Science Foundation of China","award":["CARS36"],"award-info":[{"award-number":["CARS36"]}]},{"name":"National Natural Science Foundation of China","award":["ASTIP-2016-IAS-06"],"award-info":[{"award-number":["ASTIP-2016-IAS-06"]}]},{"name":"National Natural Science Foundation of China","award":["21626604D"],"award-info":[{"award-number":["21626604D"]}]},{"name":"National Natural Science Foundation of China","award":["21326608D"],"award-info":[{"award-number":["21326608D"]}]},{"name":"National Natural Science Foundation of China","award":["G2022003015L"],"award-info":[{"award-number":["G2022003015L"]}]},{"DOI":"10.13039\/501100004826","name":"Natural Science Foundation of Beijing","doi-asserted-by":"publisher","award":["2022YFE0100200"],"award-info":[{"award-number":["2022YFE0100200"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004826","name":"Natural Science Foundation of Beijing","doi-asserted-by":"publisher","award":["32161143032"],"award-info":[{"award-number":["32161143032"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004826","name":"Natural Science Foundation of Beijing","doi-asserted-by":"publisher","award":["31972568"],"award-info":[{"award-number":["31972568"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004826","name":"Natural Science Foundation of Beijing","doi-asserted-by":"publisher","award":["6202027"],"award-info":[{"award-number":["6202027"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004826","name":"Natural Science Foundation of Beijing","doi-asserted-by":"publisher","award":["CARS36"],"award-info":[{"award-number":["CARS36"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004826","name":"Natural Science Foundation of Beijing","doi-asserted-by":"publisher","award":["ASTIP-2016-IAS-06"],"award-info":[{"award-number":["ASTIP-2016-IAS-06"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004826","name":"Natural Science Foundation of Beijing","doi-asserted-by":"publisher","award":["21626604D"],"award-info":[{"award-number":["21626604D"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004826","name":"Natural Science Foundation of Beijing","doi-asserted-by":"publisher","award":["21326608D"],"award-info":[{"award-number":["21326608D"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004826","name":"Natural Science Foundation of Beijing","doi-asserted-by":"publisher","award":["G2022003015L"],"award-info":[{"award-number":["G2022003015L"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]},{"name":"CARS","award":["2022YFE0100200"],"award-info":[{"award-number":["2022YFE0100200"]}]},{"name":"CARS","award":["32161143032"],"award-info":[{"award-number":["32161143032"]}]},{"name":"CARS","award":["31972568"],"award-info":[{"award-number":["31972568"]}]},{"name":"CARS","award":["6202027"],"award-info":[{"award-number":["6202027"]}]},{"name":"CARS","award":["CARS36"],"award-info":[{"award-number":["CARS36"]}]},{"name":"CARS","award":["ASTIP-2016-IAS-06"],"award-info":[{"award-number":["ASTIP-2016-IAS-06"]}]},{"name":"CARS","award":["21626604D"],"award-info":[{"award-number":["21626604D"]}]},{"name":"CARS","award":["21326608D"],"award-info":[{"award-number":["21326608D"]}]},{"name":"CARS","award":["G2022003015L"],"award-info":[{"award-number":["G2022003015L"]}]},{"name":"National Germplasm Center of Domestic Animal Resources and the Agricultural Science and Technology Innovation Program","award":["2022YFE0100200"],"award-info":[{"award-number":["2022YFE0100200"]}]},{"name":"National Germplasm Center of Domestic Animal Resources and the Agricultural Science and Technology Innovation Program","award":["32161143032"],"award-info":[{"award-number":["32161143032"]}]},{"name":"National Germplasm Center of Domestic Animal Resources and the Agricultural Science and Technology Innovation Program","award":["31972568"],"award-info":[{"award-number":["31972568"]}]},{"name":"National Germplasm Center of Domestic Animal Resources and the Agricultural Science and Technology Innovation Program","award":["6202027"],"award-info":[{"award-number":["6202027"]}]},{"name":"National Germplasm Center of Domestic Animal Resources and the Agricultural Science and Technology Innovation Program","award":["CARS36"],"award-info":[{"award-number":["CARS36"]}]},{"name":"National Germplasm Center of Domestic Animal Resources and the Agricultural Science and Technology Innovation Program","award":["ASTIP-2016-IAS-06"],"award-info":[{"award-number":["ASTIP-2016-IAS-06"]}]},{"name":"National Germplasm Center of Domestic Animal Resources and the Agricultural Science and Technology Innovation Program","award":["21626604D"],"award-info":[{"award-number":["21626604D"]}]},{"name":"National Germplasm Center of Domestic Animal Resources and the Agricultural Science and Technology Innovation Program","award":["21326608D"],"award-info":[{"award-number":["21326608D"]}]},{"name":"National Germplasm Center of Domestic Animal Resources and the Agricultural Science and Technology Innovation Program","award":["G2022003015L"],"award-info":[{"award-number":["G2022003015L"]}]},{"name":"Transformation Fund Program for Agricultural Science and Technology Achievements of Hebei Province","award":["2022YFE0100200"],"award-info":[{"award-number":["2022YFE0100200"]}]},{"name":"Transformation Fund Program for Agricultural Science and Technology Achievements of Hebei Province","award":["32161143032"],"award-info":[{"award-number":["32161143032"]}]},{"name":"Transformation Fund Program for Agricultural Science and Technology Achievements of Hebei Province","award":["31972568"],"award-info":[{"award-number":["31972568"]}]},{"name":"Transformation Fund Program for Agricultural Science and Technology Achievements of Hebei Province","award":["6202027"],"award-info":[{"award-number":["6202027"]}]},{"name":"Transformation Fund Program for Agricultural Science and Technology Achievements of Hebei Province","award":["CARS36"],"award-info":[{"award-number":["CARS36"]}]},{"name":"Transformation Fund Program for Agricultural Science and Technology Achievements of Hebei Province","award":["ASTIP-2016-IAS-06"],"award-info":[{"award-number":["ASTIP-2016-IAS-06"]}]},{"name":"Transformation Fund Program for Agricultural Science and Technology Achievements of Hebei Province","award":["21626604D"],"award-info":[{"award-number":["21626604D"]}]},{"name":"Transformation Fund Program for Agricultural Science and Technology Achievements of Hebei Province","award":["21326608D"],"award-info":[{"award-number":["21326608D"]}]},{"name":"Transformation Fund Program for Agricultural Science and Technology Achievements of Hebei Province","award":["G2022003015L"],"award-info":[{"award-number":["G2022003015L"]}]},{"DOI":"10.13039\/501100015286","name":"Key Research and Development Program of Hebei Province","doi-asserted-by":"publisher","award":["2022YFE0100200"],"award-info":[{"award-number":["2022YFE0100200"]}],"id":[{"id":"10.13039\/501100015286","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100015286","name":"Key Research and Development Program of Hebei Province","doi-asserted-by":"publisher","award":["32161143032"],"award-info":[{"award-number":["32161143032"]}],"id":[{"id":"10.13039\/501100015286","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100015286","name":"Key Research and Development Program of Hebei Province","doi-asserted-by":"publisher","award":["31972568"],"award-info":[{"award-number":["31972568"]}],"id":[{"id":"10.13039\/501100015286","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100015286","name":"Key Research and Development Program of Hebei Province","doi-asserted-by":"publisher","award":["6202027"],"award-info":[{"award-number":["6202027"]}],"id":[{"id":"10.13039\/501100015286","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100015286","name":"Key Research and Development Program of Hebei Province","doi-asserted-by":"publisher","award":["CARS36"],"award-info":[{"award-number":["CARS36"]}],"id":[{"id":"10.13039\/501100015286","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100015286","name":"Key Research and Development Program of Hebei Province","doi-asserted-by":"publisher","award":["ASTIP-2016-IAS-06"],"award-info":[{"award-number":["ASTIP-2016-IAS-06"]}],"id":[{"id":"10.13039\/501100015286","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100015286","name":"Key Research and Development Program of Hebei Province","doi-asserted-by":"publisher","award":["21626604D"],"award-info":[{"award-number":["21626604D"]}],"id":[{"id":"10.13039\/501100015286","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100015286","name":"Key Research and Development Program of Hebei Province","doi-asserted-by":"publisher","award":["21326608D"],"award-info":[{"award-number":["21326608D"]}],"id":[{"id":"10.13039\/501100015286","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100015286","name":"Key Research and Development Program of Hebei Province","doi-asserted-by":"publisher","award":["G2022003015L"],"award-info":[{"award-number":["G2022003015L"]}],"id":[{"id":"10.13039\/501100015286","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Development and demonstration of Holstein Breeding and ovum pick-up and in vitro Fertilization (OPU-IVF) technology","award":["2022YFE0100200"],"award-info":[{"award-number":["2022YFE0100200"]}]},{"name":"Development and demonstration of Holstein Breeding and ovum pick-up and in vitro Fertilization (OPU-IVF) technology","award":["32161143032"],"award-info":[{"award-number":["32161143032"]}]},{"name":"Development and demonstration of Holstein Breeding and ovum pick-up and in vitro Fertilization (OPU-IVF) technology","award":["31972568"],"award-info":[{"award-number":["31972568"]}]},{"name":"Development and demonstration of Holstein Breeding and ovum pick-up and in vitro Fertilization (OPU-IVF) technology","award":["6202027"],"award-info":[{"award-number":["6202027"]}]},{"name":"Development and demonstration of Holstein Breeding and ovum pick-up and in vitro Fertilization (OPU-IVF) technology","award":["CARS36"],"award-info":[{"award-number":["CARS36"]}]},{"name":"Development and demonstration of Holstein Breeding and ovum pick-up and in vitro Fertilization (OPU-IVF) technology","award":["ASTIP-2016-IAS-06"],"award-info":[{"award-number":["ASTIP-2016-IAS-06"]}]},{"name":"Development and demonstration of Holstein Breeding and ovum pick-up and in vitro Fertilization (OPU-IVF) technology","award":["21626604D"],"award-info":[{"award-number":["21626604D"]}]},{"name":"Development and demonstration of Holstein Breeding and ovum pick-up and in vitro Fertilization (OPU-IVF) technology","award":["21326608D"],"award-info":[{"award-number":["21326608D"]}]},{"name":"Development and demonstration of Holstein Breeding and ovum pick-up and in vitro Fertilization (OPU-IVF) technology","award":["G2022003015L"],"award-info":[{"award-number":["G2022003015L"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>Oocyte maturation is a critical step in the completion of female gametogenesis in the ovary; thus, for subsequent fertilization and embryogenesis. Vitrification of embryo also has been shown to be closely associated with oocyte maturation. To improve the quality and developmental potential of bovine oocytes derived from in vitro maturation (IVM), Pre-IVM with C-type natriuretic peptide (CNP), melatonin (MT) and in combination, IGF1, FGF2, LIF (FLI) were supplemented in the IVM medium. In this current study, we cultured bovine oocytes in Pre-IVM with CNP for 6 h before transferring them to the IVM medium supplemented with MT and FLI. The developmental potential of bovine oocytes was then investigated by measuring the reactive oxygen species (ROS), the intracellular glutathione (GSH) and ATP levels, the transzonal projections (TZP), the mitochondrial membrane potential (\u0394\u03a8m), cacline-AM, and the expression of related genes (cumulus cells (CCs), oocytes, blastocysts). The results revealed that oocytes treated with a combination of CNP, MT, and FLI had dramatically improved the percentage of oocytes developed to blastocyst, ATP content, GSH levels, TZP intensity, the \u0394\u03a8m, cacline-AM fluorescence intensity, and considerably reduced ROS levels of oocytes. Furthermore, the survival rate and the hatched rate after vitrification of the CNP+MT+FLI group were significantly higher than those other groups. Thus, we speculated that CNP+MT+FLI increases the IVM of bovine oocytes. In conclusion, our findings deepen our understanding and provide new perspectives on targeting the combination of CNP, MT and FLI to enhance the quality and developmental potential of bovine oocytes.<\/jats:p>","DOI":"10.3390\/antiox12040897","type":"journal-article","created":{"date-parts":[[2023,4,10]],"date-time":"2023-04-10T03:49:29Z","timestamp":1681098569000},"page":"897","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Combination of CNP, MT and FLI during IVM Significantly Improved the Quality and Development Abilities of Bovine Oocytes and IVF-Derived Embryos"],"prefix":"10.3390","volume":"12","author":[{"given":"Peipei","family":"Zhang","sequence":"first","affiliation":[{"name":"Institute of Animal Sciences (IAS), Chinese Academy of Agricultural Sciences (CAAS), No. 2 Yuanmingyuan Western Road, Haidian District, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baigao","family":"Yang","sequence":"additional","affiliation":[{"name":"Institute of Animal Sciences (IAS), Chinese Academy of Agricultural Sciences (CAAS), No. 2 Yuanmingyuan Western Road, Haidian District, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi","family":"Xu","sequence":"additional","affiliation":[{"name":"Institute of Animal Sciences (IAS), Chinese Academy of Agricultural Sciences (CAAS), No. 2 Yuanmingyuan Western Road, Haidian District, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Animal Sciences (IAS), Chinese Academy of Agricultural Sciences (CAAS), No. 2 Yuanmingyuan Western Road, Haidian District, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyi","family":"Feng","sequence":"additional","affiliation":[{"name":"Institute of Animal Sciences (IAS), Chinese Academy of Agricultural Sciences (CAAS), No. 2 Yuanmingyuan Western Road, Haidian District, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haisheng","family":"Hao","sequence":"additional","affiliation":[{"name":"Institute of Animal Sciences (IAS), Chinese Academy of Agricultural Sciences (CAAS), No. 2 Yuanmingyuan Western Road, Haidian District, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1248-4471","authenticated-orcid":false,"given":"Weihua","family":"Du","sequence":"additional","affiliation":[{"name":"Institute of Animal Sciences (IAS), Chinese Academy of Agricultural Sciences (CAAS), No. 2 Yuanmingyuan Western Road, Haidian District, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huabin","family":"Zhu","sequence":"additional","affiliation":[{"name":"Institute of Animal Sciences (IAS), Chinese Academy of Agricultural Sciences (CAAS), No. 2 Yuanmingyuan Western Road, Haidian District, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shujing","family":"Li","sequence":"additional","affiliation":[{"name":"Shijiazhuang Tianquan Elite Dairy Ltd., Shijiazhuang 050200, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenli","family":"Yu","sequence":"additional","affiliation":[{"name":"Shijiazhuang Tianquan Elite Dairy Ltd., Shijiazhuang 050200, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8165-1811","authenticated-orcid":false,"given":"Adnan","family":"Khan","sequence":"additional","affiliation":[{"name":"Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4525-0823","authenticated-orcid":false,"given":"Saqib","family":"Umer","sequence":"additional","affiliation":[{"name":"Department of Theriogenology, University of Agriculture, Faisalabad 38000, Punjab, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6110-2690","authenticated-orcid":false,"given":"Xueming","family":"Zhao","sequence":"additional","affiliation":[{"name":"Institute of Animal Sciences (IAS), Chinese Academy of Agricultural Sciences (CAAS), No. 2 Yuanmingyuan Western Road, Haidian District, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,4,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1016\/j.theriogenology.2013.04.025","article-title":"Cysteamine supplementation during in vitro maturation of slaughterhouse- and opu-derived bovine oocytes improves embryonic development without affecting cryotolerance, pregnancy rate, and calf characteristics","volume":"80","author":"Merton","year":"2013","journal-title":"Theriogenology"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Balboula, A.Z., Aboelenain, M., Sakatani, M., Yamanaka, K.I., Bai, H., Shirozu, T., Kawahara, M., Hegab, A.E.O., Zaabel, S.M., and Takahashi, M. (2022). Effect of E-64 Supplementation during in vitro Maturation on the Developmental Competence of Bovine OPU-Derived Oocytes. Genes, 13.","DOI":"10.3390\/genes13020324"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1037","DOI":"10.1111\/j.1439-0531.2012.02010.x","article-title":"Bovine OPU-derived oocytes can be matured in vitro for 16-28 h with similar developmental capacity","volume":"47","author":"Merton","year":"2012","journal-title":"Reprod. Domest. Anim."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1016\/j.theriogenology.2018.03.002","article-title":"Variations in bovine embryo production between individual donors for OPU-IVF are closely related to glutathione concentrations in oocytes during in vitro maturation","volume":"113","author":"Hidaka","year":"2018","journal-title":"Theriogenology"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1186\/1477-7827-9-121","article-title":"Improved blastocyst development of single cow OPU-derived presumptive zygotes by group culture with agarose-embedded helper embryos","volume":"9","author":"Deb","year":"2011","journal-title":"Reprod. Biol. Endocrinol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1262\/jrd.2019-022","article-title":"Acquisition of developmental competence and in vitro growth culture of bovine oocytes","volume":"65","author":"Nagano","year":"2019","journal-title":"J. Reprod. Dev."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.theriogenology.2020.05.030","article-title":"Synchronization of germinal vesicle maturity improves efficacy of in vitro embryo production in Holstein cows","volume":"154","author":"Soares","year":"2020","journal-title":"Theriogenology"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/j.theriogenology.2021.09.016","article-title":"The effect of Tempo and MitoTEMPO on oocyte maturation and subsequent embryo development in bovine model","volume":"176","author":"Yousefian","year":"2021","journal-title":"Theriogenology"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.anireprosci.2019.01.007","article-title":"Effect of pre-maturation with C-type natriuretic peptide and 3-isobutyl-1-methylxanthine on cumulus-oocyte com-munication and oocyte developmental competence in cattle","volume":"202","author":"Paramio","year":"2019","journal-title":"Anim. Reprod. Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1016\/j.pneurobio.2010.12.004","article-title":"Melatonin\u2014A pleiotropic, orchestrating regulator molecule","volume":"93","author":"Hardeland","year":"2011","journal-title":"Prog. Neurobiol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"119317","DOI":"10.1016\/j.envpol.2022.119317","article-title":"Nonylphenol exposure-induced oocyte quality deterioration could be reversed by melatonin supplementation in mice","volume":"305","author":"Hu","year":"2022","journal-title":"Environ. Pollut."},{"key":"ref_12","first-page":"244","article-title":"Melatonin modifies histone acetylation during in vitro maturation of mouse oocytes","volume":"20","author":"Keshavarzi","year":"2018","journal-title":"Cell J."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Yang, M., Tao, J., Chai, M., Wu, H., Wang, J., Li, G., He, C., Xie, L., Ji, P., and Dai, Y. (2017). Melatonin improves the quality of inferior bovine oocytes and promoted their subsequent IVF embryo development: Mechanisms and results. Molecules, 22.","DOI":"10.3390\/molecules22122059"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1151","DOI":"10.21451\/1984-3143-AR987","article-title":"Leptin and IGF-I improve bovine embryo quality in vitro","volume":"14","author":"Kaya","year":"2017","journal-title":"Anim. Reprod."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1262\/jrd.2017-145","article-title":"Effects of insulin-like growth factor-1 on the in vitro maturation of canine oocytes","volume":"64","author":"Sato","year":"2018","journal-title":"J. Reprod. Dev."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"905","DOI":"10.1007\/s10815-019-01436-7","article-title":"Fibroblast growth factor 2 regulates cumulus differentiation under the control of the oocyte","volume":"36","author":"Barros","year":"2019","journal-title":"J. Assist. Reprod. Genet."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1002\/mrd.22289","article-title":"Leukemia inhibitory factor promotes porcine oocyte maturation and is accompanied by activation of signal transducer and activator of transcription 3","volume":"81","author":"Haraguchi","year":"2014","journal-title":"Mol. Reprod. Dev."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"608","DOI":"10.1002\/mrd.22327","article-title":"Leukemia inhibitory factor enhances bovine oocyte maturation and early embryo development","volume":"81","author":"Mo","year":"2014","journal-title":"Mol. Reprod. Dev."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"E5796","DOI":"10.1073\/pnas.1703998114","article-title":"Quadrupling efficiency in production of genetically modified pigs through improved oocyte maturation","volume":"114","author":"Yuan","year":"2017","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"801","DOI":"10.1017\/S0967199422000284","article-title":"Supplementation of porcine in vitromaturation medium with FGF2, LIF, and IGF1 enhances cytoplasmic maturation in prepubertal gilts oocytes and improves embryo quality","volume":"30","author":"Silvestri","year":"2022","journal-title":"Zygote"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/j.theriogenology.2022.10.005","article-title":"Optimizing swine in vitro embryo production with growth factor and antioxidant supplementation during oocyte maturation","volume":"194","author":"Currin","year":"2022","journal-title":"Theriogenology"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.theriogenology.2020.10.019","article-title":"Enhancing the developmental competence of prepubertal lamb oocytes by supplementing the in vitro maturation medium with sericin and the fibroblast growth factor 2\u2014Leukemia inhibitory factor\u2013Insulin-like growth factor 1 combination","volume":"159","author":"Tian","year":"2021","journal-title":"Theriogenology"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1095\/biolreprod12.2.260","article-title":"Capacitation of Rabbit Spermatozoa in vitro","volume":"12","author":"Brackett","year":"1975","journal-title":"Biol. Reprod."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1111\/jpi.12290","article-title":"Melatonin inhibits apoptosis and improves the developmental potential of vitrified bovine oocytes","volume":"60","author":"Zhao","year":"2016","journal-title":"J. Pineal. Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1071\/RD02063","article-title":"Effect of different vitrification protocols for human ovarian tissue on reactive oxygen species and apoptosis","volume":"15","author":"Rahimi","year":"2003","journal-title":"Reprod. Fertil. Dev."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"212445","DOI":"10.1111\/jpi.12445","article-title":"Melatonin improves the fertilization capacity and developmental ability of bovine oocytes by regulating cytoplasmic maturation events","volume":"64","author":"Zhao","year":"2018","journal-title":"J. Pineal. Res."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1530\/rep.0.1240683","article-title":"Developmental competence and oxidative state of mouse zygotes heat-stressed maternally or in vitro","volume":"124","author":"Ozawa","year":"2022","journal-title":"Reproduction"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"760","DOI":"10.1071\/RD21145","article-title":"Melatonin improves the maturation and developmental ability of bovine oocytes by up-regulating GJA4 to enhance gap junction intercellular communication","volume":"33","author":"Hao","year":"2021","journal-title":"Reprod. Fertil. Dev."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1016\/j.theriogenology.2017.09.003","article-title":"Natriuretic peptide receptor 2 (NPR2) localized in bovine oocyte underlies a unique mechanism for C-type natriuretic peptide (CNP)-induced meiotic arrest","volume":"106","author":"Xi","year":"2018","journal-title":"Theriogenology"},{"key":"ref_30","first-page":"173","article-title":"Pre-IVM treatment with C-type natriuretic peptide in the presence of cysteamine enhances bovine oocytes antioxidant defense ability and developmental competence in vitro","volume":"20","author":"Zhenwei","year":"2019","journal-title":"Iran. J. Vet. Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"106685","DOI":"10.1016\/j.anireprosci.2020.106685","article-title":"Treatment of cattle oocytes with C-type natriuretic peptide before in vitro maturation enhances oocyte mitochondrial function","volume":"225","author":"Jia","year":"2021","journal-title":"Anim. Reprod. Sci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1126\/science.1193573","article-title":"Granulosa cell ligand NPPC and its receptor NPR2 maintain meiotic arrest in mouse oocytes","volume":"330","author":"Zhang","year":"2010","journal-title":"Science"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1007\/s10815-013-0021-2","article-title":"Supplementation with low concentrations of melatonin improves nuclear maturation of human oocytes in vitro","volume":"30","author":"Wei","year":"2013","journal-title":"J. Assist. Reprod. Genet."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1017\/S0967199414000161","article-title":"Melatonin enhances the in vitro maturation and developmental potential of bovine oocytes denuded of the cumulus oophorus","volume":"23","author":"Zhao","year":"2015","journal-title":"Zygote"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1016\/j.theriogenology.2020.09.039","article-title":"Melatonin slightly alleviates the effect of heat shock on bovine oocytes and resulting blastocysts","volume":"158","author":"Shimoni","year":"2020","journal-title":"Theriogenology"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"e12458","DOI":"10.1111\/jpi.12458","article-title":"Melatonin improves the meiotic maturation of porcine oocytes by reducing endoplasmic reticulum stress during in vitro maturation","volume":"64","author":"Park","year":"2018","journal-title":"J. Pineal. Res."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Martinez, C.A., Cuello, C., Parrilla, I., Maside, C., Ramis, G., Cambra, J.M., Vazquez, J.M., Rodriguez-Martinez, H., Gil, M.A., and Martinez, E.A. (2022). Exogenous Melatonin in the Culture Medium Does Not Affect the Development of In Vivo-Derived Pig Embryos but Substantially Improves the Quality of In Vitro-Produced Embryos. Antioxidants, 11.","DOI":"10.3390\/antiox11061177"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Proch\u00e1zka, R., Bartkov\u00e1, A., N\u011bmcov\u00e1, L., Mur\u00edn, M., Gad, A., Marcollov\u00e1, K., Kinterov\u00e1, V., Lucas-Hahn, A., and Laurin\u010d\u00edk, J. (2021). The Role of MAPK3\/1 and AKT in the Acquisition of High Meiotic and Developmental Competence of Porcine Oocytes Cultured In Vitro in FLI Medium. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms222011148"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/j.cryobiol.2016.02.002","article-title":"C-type natriuretic peptide improved vitrified-warmed mouse cumulus oocyte complexes developmental competence","volume":"72","author":"Yang","year":"2016","journal-title":"Cryobiology"},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Stoecklein Stoecklein, K.S., Ortega, M.S., Spate, L.D., Murphy, C.N., and Prather, R.S. (2021). Improved cryopreservation of in vitro produced bovine embryos using FGF2, LIF, and IGF1. PLoS ONE, 16.","DOI":"10.1371\/journal.pone.0243727"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"2973","DOI":"10.2337\/db07-0510","article-title":"Essential role of mitochondrial function in adiponectin synthesis in adipocytes","volume":"56","author":"Koh","year":"2007","journal-title":"Diabetes"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/j.neuron.2014.12.007","article-title":"The roles of PINK1, parkin, and mitochondrial fidelity in Parkinson\u2019s disease","volume":"85","author":"Pickrell","year":"2015","journal-title":"Neuron"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1111\/j.1432-1033.1991.tb15945.x","article-title":"The respiratory-chain NADH dehydrogenase (Complex I) of mitochondria","volume":"197","author":"Weiss","year":"1991","journal-title":"Eur. J. Biochem."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/j.mad.2016.04.007","article-title":"A brewing understanding of the regulation of Bax function by Bcl-xL and Bcl-2","volume":"161","author":"Renault","year":"2017","journal-title":"Mech. Ageing Dev."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1536","DOI":"10.1093\/humrep\/det086","article-title":"Heparin and cAMP modulators interact during pre-in vitro maturation to affect mouse and human oocyte meiosis and developmental competence","volume":"28","author":"Zeng","year":"2013","journal-title":"Hum. Reprod."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1007\/s11626-016-0101-6","article-title":"Effect of c-type natriuretic peptide pretreatment on in vitro bovine oocyte maturation","volume":"53","author":"Zhang","year":"2017","journal-title":"Vitr. Cell Dev. Biol. Anim."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"102215","DOI":"10.1016\/j.redox.2021.102215","article-title":"Melatonin improves the quality of maternally aged oocytes by maintaining intercellular communication and antioxidant metabolite supply","volume":"49","author":"Zhang","year":"2022","journal-title":"Redox. Biol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"e12627","DOI":"10.1111\/jpi.12627","article-title":"Melatonin enhances mitochondrial biogenesis and protects against rotenone-induced mitochondrial deficiency in early porcine embryos","volume":"68","author":"Niu","year":"2020","journal-title":"J. Pineal. Res."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"e12388","DOI":"10.1111\/jpi.12388","article-title":"Melatonin regulates lipid metabolism in porcine oocytes","volume":"62","author":"Jin","year":"2017","journal-title":"J. Pineal. Res."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Yang, S., Yang, Y., Hao, H., Du, W., Pang, Y., Zhao, S., Zou, H., Zhu, H., Zhang, P., and Zhao, X. (2022). Supplementation of EGF, IGF-1, and Connexin 37 in IVM Medium Significantly Improved the Maturation of Bovine Oocytes and Vitrification of Their IVF Blastocysts. Genes, 13.","DOI":"10.3390\/genes13050805"},{"key":"ref_51","first-page":"775","article-title":"The effect of insulin-like growth factor-I (IGF-I) on mitochondrial gene expression of bovine oocytes subjected to heat shock","volume":"12","author":"Ticianelli","year":"2015","journal-title":"Anim. Reprod."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1262","DOI":"10.1038\/s41598-018-37766-2","article-title":"Three-dimensional organization of transzonal projections and other cytoplasmic extensions in the mouse ovarian follicle","volume":"9","author":"Baena","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1071\/RDv17n2paperabs","article-title":"Mouse oocytes control metabolic co-operativity between oocytes and cumulus cells","volume":"17","author":"Eppig","year":"2005","journal-title":"Reprod. Fertil. Dev."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"R143","DOI":"10.1530\/REP-15-0606","article-title":"Oocyte maturation and quality: Role of cyclic nucleotides","volume":"152","author":"Gilchrist","year":"2016","journal-title":"Reproduction"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1017\/S0967199421000393","article-title":"Assessment of cGMP level in medium during in vitro growth period of murine preantral follicles with and without supplementation of C-type natriuretic peptide","volume":"30","author":"Ang","year":"2022","journal-title":"Zygote"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1095\/biolreprod.113.114132","article-title":"New elements in the C-type natriuretic peptide signaling pathway inhibiting swine in vitro oocyte meiotic resumption","volume":"91","author":"Santiquet","year":"2014","journal-title":"Biol. Reprod."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"2217","DOI":"10.1071\/RD16320","article-title":"Steroid hormones interact with natriuretic peptide C to delay nuclear maturation, to maintain oocyte-cumulus communication and to improve the quality of in vitro-produced embryos in cattle","volume":"29","author":"Soares","year":"2017","journal-title":"Reprod. Fertil. Dev."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/S0070-2153(06)77003-X","article-title":"Mitochondrial DNA in the oocyte and the developing embryo","volume":"77","author":"Chretien","year":"2007","journal-title":"Curr. Top. Dev. Biol."},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Tan, D.X., Manchester, L.C., Qin, L., and Reiter, R.J. (2016). Melatonin: A Mitochondrial Targeting Molecule Involving Mitochondrial Protection and Dynamics. Int. J. Mol. Sci., 17.","DOI":"10.3390\/ijms17122124"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1002\/mrd.21295","article-title":"Evidence of melatonin synthesis in the cumulus oocyte complexes and its role in enhancing oocyte maturation in vitro in cattle","volume":"78","author":"Geshi","year":"2011","journal-title":"Mol. Reprod. Dev."},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Rouhollahi, V.S., Hajian, M., Jafarpour, F., Ghazvini, Z.F., and Nasr-Esfahani, M.H. (2020). Granulosa secreted factors improve the developmental competence of cumulus oocyte complexes from small antral follicles in sheep. PLoS ONE, 15.","DOI":"10.1371\/journal.pone.0229043"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"gaab051","DOI":"10.1093\/molehr\/gaab051","article-title":"Leukaemia inhibitory factor modulates the differentiation of granulosa cells during sheep in vitro preantral to antral follicle development and improves oocyte meiotic competence","volume":"27","author":"Cadoret","year":"2021","journal-title":"Mol. Hum. Reprod."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"474","DOI":"10.1093\/biolre\/ioac078","article-title":"MYO10 promotes transzonal projection-dependent germ line-somatic contact during mammalian folliculogenesis\u2020","volume":"107","author":"Yang","year":"2022","journal-title":"Biol. Reprod."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"590","DOI":"10.1016\/j.ceb.2004.07.009","article-title":"Roles of fascin in cell adhesion and motility","volume":"16","author":"Adams","year":"2004","journal-title":"Curr. Opin. Cell Biol."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1016\/j.crvi.2018.03.001","article-title":"First evidence of DAAM1 localization in mouse seminal vesicles and its possible involvement during regulated exocytosis","volume":"341","author":"Venditti","year":"2018","journal-title":"C R Biol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1083\/jcb.201602060","article-title":"ARHGEF17 sets the timer for retention of Mps1 at kinetochores","volume":"212","author":"Marquardt","year":"2016","journal-title":"J. Cell Biol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"2261","DOI":"10.1242\/dev.061317","article-title":"Mps1 at kinetochores is essential for female mouse meiosis I","volume":"138","author":"Hached","year":"2011","journal-title":"Development"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1093\/humupd\/dmr044","article-title":"Spindle assembly checkpoint and its regulators in meiosis","volume":"18","author":"Sun","year":"2012","journal-title":"Hum. Reprod. Update."},{"key":"ref_69","doi-asserted-by":"crossref","unstructured":"Wu, Z., Pan, B., Qazi, I.H., Yang, H., Guo, S., Yang, J., Zhang, Y., Zeng, C., Zhang, M., and Han, H. (2019). Melatonin Improves In Vitro Development of Vitrified-Warmed Mouse Germinal Vesicel Oocytes Potentially via Modulation of Spindle Assembly Checkpoint-Related Genes. Cells, 8.","DOI":"10.3390\/cells8091009"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1007\/s10815-010-9481-9","article-title":"The cumulus cell gene expression profile of oocytes with different nuclear maturity and potential for blastocyst formation","volume":"28","author":"Adriaenssens","year":"2011","journal-title":"J. Assist. Rerod. Genet."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"388","DOI":"10.1016\/j.rbmo.2013.11.005","article-title":"Role of PTGS2-generated PGE2 during gonadotrophin-induced bovine oocyte maturation and cumulus cell expansion","volume":"28","author":"Marei","year":"2014","journal-title":"Reprod. Biomed. Online."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1095\/biolreprod65.1.135","article-title":"Cyclooxygenase-2 and prostaglandin E(2) (PGE(2)) receptor messenger RNAs are affected by bovine oocyte maturation time and cumulus-oocyte complex quality, and PGE(2) induces moderate expansion of the bovine cumulus in vitro","volume":"65","author":"Calder","year":"2001","journal-title":"Biol. Reprod."},{"key":"ref_73","first-page":"108","article-title":"Evaluating the Effect of Melatonin on HAS2, and PGR expression, as well as Cumulus Expansion, and Fertility Potential in Mice","volume":"20","author":"Ezzati","year":"2018","journal-title":"Cell J."},{"key":"ref_74","doi-asserted-by":"crossref","unstructured":"Tian, X., Wang, F., Zhang, L., He, C., Ji, P., Wang, J., Zhang, Z., Dongying, L., Ab-ulizi, W., and Wang, X. (2017). Beneficial effects of melatonin on the in vitro maturation of sheep oocytes and its relation to melatonin receptors. Int. J. Mol. Sci., 18.","DOI":"10.3390\/ijms18040834"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1530\/REP-20-0264","article-title":"FGF2\/FGFR signaling promotes cumulus-oocyte complex maturation in vitro","volume":"161","author":"Du","year":"2021","journal-title":"Reproduction"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"1493","DOI":"10.1007\/s10815-017-0998-z","article-title":"Temporal expression of cumulus cell marker genes during in vitro maturation and oocyte developmental competence","volume":"34","author":"Dhali","year":"2017","journal-title":"J. Assist. Reprod. Genet."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1017\/S0967199421000058","article-title":"Physical parameters of bovine activated oocytes and zygotes as predictors of development success","volume":"29","author":"Timlin","year":"2021","journal-title":"Zygote"},{"key":"ref_78","doi-asserted-by":"crossref","unstructured":"Gorczyca, G., Wartalski, K., Romek, M., Samiec, M., and Duda, M. (2022). The Molecular Quality and Mitochondrial Activity of Porcine Cumulus-Oocyte Complexes Are Affected by Their Exposure to Three Endocrine-Active Compounds under 3D In Vitro Maturation Conditions. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23094572"},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"749","DOI":"10.1017\/S0967199422000296","article-title":"Bovine ICSI: Limiting factors, strategies to improve its efficiency and alternative approaches","volume":"30","author":"Fuentes","year":"2022","journal-title":"Zygote"},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"106925","DOI":"10.1016\/j.anireprosci.2022.106925","article-title":"Past, present and future of ICSI in livestock species","volume":"246","author":"Briski","year":"2022","journal-title":"Anim. Reprod. Sci."},{"key":"ref_81","doi-asserted-by":"crossref","unstructured":"Skrzyszowska, M., and Samiec, M. (2021). Generating Cloned Goats by Somatic Cell Nuclear Transfer\u2014Molecular Determinants and Application to Transgenics and Biomedicine. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22147490"},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"509","DOI":"10.2478\/v10181-012-0078-3","article-title":"Pseudophysiological transcomplementary activation of reconstructed oocytes as a highly efficient method used for producing nuclear-transferred pig embryos originating from transgenic foetal fibroblast cells","volume":"15","author":"Samiec","year":"2012","journal-title":"Pol. J. Vet. Sci."}],"container-title":["Antioxidants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-3921\/12\/4\/897\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:12:03Z","timestamp":1760123523000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-3921\/12\/4\/897"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,4,7]]},"references-count":82,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2023,4]]}},"alternative-id":["antiox12040897"],"URL":"https:\/\/doi.org\/10.3390\/antiox12040897","relation":{},"ISSN":["2076-3921"],"issn-type":[{"value":"2076-3921","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,4,7]]}}}