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The data were acquired at the Metabolomics Core Facility at the Institute of Physiology of the Czech Academy of Sciences","award":["NU20-01-00186"],"award-info":[{"award-number":["NU20-01-00186"]}]},{"name":"the program Projects of Large Research, Development, and Innovations Infrastructures. The data were acquired at the Metabolomics Core Facility at the Institute of Physiology of the Czech Academy of Sciences","award":["NU22-02-00161"],"award-info":[{"award-number":["NU22-02-00161"]}]},{"name":"the program Projects of Large Research, Development, and Innovations Infrastructures. The data were acquired at the Metabolomics Core Facility at the Institute of Physiology of the Czech Academy of Sciences","award":["21-00477S"],"award-info":[{"award-number":["21-00477S"]}]},{"name":"the program Projects of Large Research, Development, and Innovations Infrastructures. The data were acquired at the Metabolomics Core Facility at the Institute of Physiology of the Czech Academy of Sciences","award":["LTAUSA19124"],"award-info":[{"award-number":["LTAUSA19124"]}]},{"name":"the program Projects of Large Research, Development, and Innovations Infrastructures. The data were acquired at the Metabolomics Core Facility at the Institute of Physiology of the Czech Academy of Sciences","award":["LX22NPO5104"],"award-info":[{"award-number":["LX22NPO5104"]}]},{"name":"the program Projects of Large Research, Development, and Innovations Infrastructures. The data were acquired at the Metabolomics Core Facility at the Institute of Physiology of the Czech Academy of Sciences","award":["LM2018140"],"award-info":[{"award-number":["LM2018140"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>Thermal reactions can significantly alter the metabolomic and lipidomic content of biofluids and tissues during storage. In this study, we investigated the stability of polar metabolites and complex lipids in dry human serum and mouse liver extracts over a three-day period under various temperature conditions. Specifically, we tested temperatures of \u221280 \u00b0C (freezer), \u221224 \u00b0C (freezer), \u22120.5 \u00b0C (polystyrene box with gel-based ice packs), +5 \u00b0C (refrigerator), +23 \u00b0C (laboratory, room temperature), and +30 \u00b0C (thermostat) to simulate the time between sample extraction and analysis, shipping dry extracts to different labs as an alternative to dry ice, and document the impact of higher temperatures on sample integrity. The extracts were analyzed using five fast liquid chromatography-mass spectrometry (LC-MS) methods to screen polar metabolites and complex lipids, and over 600 metabolites were annotated in serum and liver extracts. We found that storing dry extracts at \u221224 \u00b0C and partially at \u22120.5 \u00b0C provided comparable results to \u221280 \u00b0C (reference condition). However, increasing the storage temperatures led to significant changes in oxidized triacylglycerols, phospholipids, and fatty acids within three days. Polar metabolites were mainly affected at storage temperatures of +23 \u00b0C and +30 \u00b0C.<\/jats:p>","DOI":"10.3390\/antiox12050986","type":"journal-article","created":{"date-parts":[[2023,4,24]],"date-time":"2023-04-24T06:20:42Z","timestamp":1682317242000},"page":"986","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Short-Term Stability of Serum and Liver Extracts for Untargeted Metabolomics and Lipidomics"],"prefix":"10.3390","volume":"12","author":[{"given":"Jiri","family":"Hricko","sequence":"first","affiliation":[{"name":"Institute of Physiology of the Czech Academy of Sciences, Videnska 1083, 14200 Prague, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucie","family":"Rudl Kulhava","sequence":"additional","affiliation":[{"name":"Institute of Physiology of the Czech Academy of Sciences, Videnska 1083, 14200 Prague, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michaela","family":"Paucova","sequence":"additional","affiliation":[{"name":"Institute of Physiology of the Czech Academy of Sciences, Videnska 1083, 14200 Prague, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michaela","family":"Novakova","sequence":"additional","affiliation":[{"name":"Institute of Physiology of the Czech Academy of Sciences, Videnska 1083, 14200 Prague, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7034-4536","authenticated-orcid":false,"given":"Ondrej","family":"Kuda","sequence":"additional","affiliation":[{"name":"Institute of Physiology of the Czech Academy of Sciences, Videnska 1083, 14200 Prague, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6261-8928","authenticated-orcid":false,"given":"Oliver","family":"Fiehn","sequence":"additional","affiliation":[{"name":"West Coast Metabolomics Center, University of California, 451 Health Sciences Drive, Davis, CA 95616, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9728-3355","authenticated-orcid":false,"given":"Tomas","family":"Cajka","sequence":"additional","affiliation":[{"name":"Institute of Physiology of the Czech Academy of Sciences, Videnska 1083, 14200 Prague, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,4,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1002\/mas.20108","article-title":"Mass spectrometry-based metabolomics","volume":"26","author":"Dettmer","year":"2007","journal-title":"Mass Spectrom. 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