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Previous studies of semi-synthetic HT-derivatives presented the hydroxytyrosyl alkyl ether HT-C6 as one of the most potent derivatives studied in the context of antioxidant, anti-platelet and antiangiogenic assays, but its direct effect on inflammation was not reported. In this work, we use RT-qPCR measure of gene expression, protein analysis by Western-blot and immunofluorescence techniques, adhesion and migration functional assays and single-cell monitoring of reactive oxygen species (ROS) in order to explore in vitro the ability of HT-C6 to interfere in the inflammatory response of endothelial cells (ECs). Our results showed that HT-C6 strongly reduces the TNF-\u03b1-induced expression of vascular cell adhesion molecule 1 (VCAM1), intercellular cell adhesion molecule 1 (ICAM1), E-selectin (SELE), C-C motif chemokine ligand 2 and 5 (CCL2 and CCL5) in HUVECs, impairing the chemotactic and adhesion potential of these cells towards THP-1 monocytes in vitro. In this work, we define a mechanism of action underlying the anti-inflammatory effect of HT-C6, which involves the abrogation of nuclear factor kappa B (NF-\u03baB) pathway activation in ECs. These results, together with the ability of HT-C6 to reduce ROS formation in ECs, point to this compound as a promising HT-derivative to be tested in the treatment of atherosclerosis.<\/jats:p>","DOI":"10.3390\/antiox12081513","type":"journal-article","created":{"date-parts":[[2023,7,28]],"date-time":"2023-07-28T07:35:24Z","timestamp":1690529724000},"page":"1513","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Inhibition of Endothelial Inflammatory Response by HT-C6, a Hydroxytyrosol Alkyl Ether Derivative"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7308-5912","authenticated-orcid":false,"given":"Ana D\u00e1cil","family":"Marrero","sequence":"first","affiliation":[{"name":"Departamento de Biolog\u00eda Molecular y Bioqu\u00edmica, Facultad de Ciencias, Universidad de M\u00e1laga, Andaluc\u00eda Tech, 29071 M\u00e1laga, Spain"},{"name":"Instituto de Investigaci\u00f3n Biom\u00e9dica de M\u00e1laga y Plataforma en Nanomedicina, IBIMA Plataforma BIONAND, 29590 M\u00e1laga, Spain"},{"name":"CIBER de Enfermedades Raras (CIBERER), Instituto de Salud Carlos III, 28029 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Castilla","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Molecular y Bioqu\u00edmica, Facultad de Ciencias, Universidad de M\u00e1laga, Andaluc\u00eda Tech, 29071 M\u00e1laga, Spain"},{"name":"Instituto de Investigaci\u00f3n Biom\u00e9dica de M\u00e1laga y Plataforma en Nanomedicina, IBIMA Plataforma BIONAND, 29590 M\u00e1laga, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0412-3035","authenticated-orcid":false,"given":"Manuel","family":"Bernal","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Molecular y Bioqu\u00edmica, Facultad de Ciencias, Universidad de M\u00e1laga, Andaluc\u00eda Tech, 29071 M\u00e1laga, Spain"},{"name":"Instituto de Investigaci\u00f3n Biom\u00e9dica de M\u00e1laga y Plataforma en Nanomedicina, IBIMA Plataforma BIONAND, 29590 M\u00e1laga, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7533-8928","authenticated-orcid":false,"given":"Inmaculada","family":"Manrique","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Molecular y Bioqu\u00edmica, Facultad de Ciencias, Universidad de M\u00e1laga, Andaluc\u00eda Tech, 29071 M\u00e1laga, Spain"},{"name":"Instituto de Investigaci\u00f3n Biom\u00e9dica de M\u00e1laga y Plataforma en Nanomedicina, IBIMA Plataforma BIONAND, 29590 M\u00e1laga, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-0354-3265","authenticated-orcid":false,"given":"Joel D.","family":"Posligua-Garc\u00eda","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda 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