{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T23:14:05Z","timestamp":1792106045742,"version":"4.4.0"},"reference-count":229,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2023,8,4]],"date-time":"2023-08-04T00:00:00Z","timestamp":1691107200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>Leydig cells are essential for steroidogenesis and spermatogenesis. An imbalance in the production of reactive oxygen species (ROS) and the cellular antioxidant level brings about oxidative stress. Oxidative stress (OS) results in the dysfunction of Leydig cells, thereby impairing steroidogenesis, spermatogenesis, and ultimately, male infertility. To prevent Leydig cells from oxidative insults, there needs to be a balance between the ROS production and the cellular protective capacity of antioxidants. Evidence indicates that medicinal plants could improve Leydig cell function at specific concentrations under basal or OS conditions. The increased usage of medicinal plants has been considered a possible alternative treatment for male infertility. This review aims to provide an overview of the impact of oxidative stress on Leydig cells as well as the effects of various medicinal plant extracts on TM3 Leydig cells. The medicinal plants of interest include Aspalathus linearis, Camellia sinensis, Moringa oleifera, Morinda officinale, Taraxacum officinale, Trichilia emetica, Terminalia sambesiaca, Peltophorum africanum, Ximenia caffra, Serenoa repens, Zingiber officinale, Eugenia jambolana, and a combination of dandelion and fermented rooibos (CRS-10). According to the findings obtained from studies conducted on the evaluated medicinal plants, it can, therefore, be concluded that the medicinal plants maintain the antioxidant profile of Leydig cells under basal conditions and have protective or restorative effects following exposure to oxidative stress. The available data suggest that the protective role exhibited by the evaluated plants may be attributed to their antioxidant content. Additionally, the use of the optimal dosage or concentration of the extracts in the management of oxidative stress is of the utmost importance, and the measurement of their oxidation reduction potential is recommended.<\/jats:p>","DOI":"10.3390\/antiox12081559","type":"journal-article","created":{"date-parts":[[2023,8,4]],"date-time":"2023-08-04T09:28:04Z","timestamp":1691141284000},"page":"1559","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":86,"title":["A Review on the Impact of Oxidative Stress and Medicinal Plants on Leydig Cells"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3607-2618","authenticated-orcid":false,"given":"Elizabeth","family":"Monageng","sequence":"first","affiliation":[{"name":"Department of Medical Biosciences, Faculty of Natural Science, University of Western Cape, Cape Town 7535, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0805-229X","authenticated-orcid":false,"given":"Ugochukwu","family":"Offor","sequence":"additional","affiliation":[{"name":"School of Anatomical Sciences, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg 2193, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3286-5458","authenticated-orcid":false,"given":"Ndivhuho Beauty","family":"Takalani","sequence":"additional","affiliation":[{"name":"Department of Medical Biosciences, Faculty of Natural Science, University of Western Cape, Cape Town 7535, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kutullo","family":"Mohlala","sequence":"additional","affiliation":[{"name":"Department of Medical Biosciences, Faculty of Natural Science, University of Western Cape, Cape Town 7535, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0804-2278","authenticated-orcid":false,"given":"Chinyerum Sylvia","family":"Opuwari","sequence":"additional","affiliation":[{"name":"Department of Medical Biosciences, Faculty of Natural Science, University of Western Cape, Cape Town 7535, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,8,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1186\/s12958-018-0341-2","article-title":"The in vitro modulation of steroidogenesis by inflammatory cytokines and insulin in TM3 Leydig cells","volume":"16","author":"Leisegang","year":"2018","journal-title":"Reprod. 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