{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T02:46:25Z","timestamp":1760150785534,"version":"build-2065373602"},"reference-count":40,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2023,12,31]],"date-time":"2023-12-31T00:00:00Z","timestamp":1703980800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>Pancreatic \u03b2-cells play a crucial role in maintaining glucose homeostasis, although they are susceptible to oxidative damage, which can ultimately impair their functionality. Thinned nectarines (TNs) have gained increasing interest due to their high polyphenol and abscisic acid (ABA) content, both of which possess antidiabetic properties. Nevertheless, the efficacy of these bioactive compounds may be compromised by limited stability and bioavailability in vivo. This study aimed to develop nanoformulations (NFs) containing pure ABA or a TN extract (TNE) at an equivalent ABA concentration. Subsequently, the insulinotropic and antioxidant potential of the NFs and their unformulated (free) forms were compared in MIN-6 pancreatic cells exposed to varying glucose (5.5 mM and 20 mM) and iron (100 \u00b5M) concentrations. NF-TNE treatment exhibited enhanced antioxidant activity compared to free TNE, while ABA-based groups showed no significant antioxidant activity. Moreover, MIN6 cells incubated with both high glucose and iron levels demonstrated significantly higher insulin AUC levels after treatment with all samples, with NF-TNE displaying the most pronounced effect. In conclusion, these results highlight the additional beneficial potential of TNE due to the synergistic combination of bioactive compounds and demonstrate the significant advantage of using a nanoformulation approach to further increase the benefits of this and similar phytobioactive molecules.<\/jats:p>","DOI":"10.3390\/antiox13010063","type":"journal-article","created":{"date-parts":[[2023,12,31]],"date-time":"2023-12-31T06:37:12Z","timestamp":1704004632000},"page":"63","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Antioxidant and Antidiabetic Properties of a Thinned-Nectarine-Based Nanoformulation in a Pancreatic \u03b2-Cell Line"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8167-0334","authenticated-orcid":false,"given":"Elisabetta","family":"Schiano","sequence":"first","affiliation":[{"name":"Department of Pharmacy, University of Naples Federico II, via Domenico Montesano 59, 80131 Naples, Italy"},{"name":"Healthcare Food Research Center, Inventia Biotech s.r.l., Strada Statale Sannitica KM 20.700, 81020 Caserta, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2181-2142","authenticated-orcid":false,"given":"Ettore","family":"Novellino","sequence":"additional","affiliation":[{"name":"Department of Medicine and Surgery, Catholic University of the Sacred Heart, 00168 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marta Mar\u00eda","family":"G\u00e1mez Fern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Centre for Nutraceuticals, School of Life Sciences, University of Westminster, 115 New Cavendish Street, London W1W 6UW, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9754-0737","authenticated-orcid":false,"given":"Helena","family":"Tiekou Lorinczova","sequence":"additional","affiliation":[{"name":"Centre for Nutraceuticals, School of Life Sciences, University of Westminster, 115 New Cavendish Street, London W1W 6UW, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0251-9936","authenticated-orcid":false,"given":"Gian Carlo","family":"Tenore","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, University of Naples Federico II, via Domenico Montesano 59, 80131 Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6340-6698","authenticated-orcid":false,"given":"Fortuna","family":"Iannuzzo","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, University of Naples Federico II, via Domenico Montesano 59, 80131 Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vinood B.","family":"Patel","sequence":"additional","affiliation":[{"name":"Centre for Nutraceuticals, School of Life Sciences, University of Westminster, 115 New Cavendish Street, London W1W 6UW, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satyanarayana","family":"Somavarapu","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics, UCL School of Pharmacy, 29-39 Brunswick Square, London WC1N 1AX, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammed Gulrez","family":"Zariwala","sequence":"additional","affiliation":[{"name":"Centre for Nutraceuticals, School of Life Sciences, University of Westminster, 115 New Cavendish Street, London W1W 6UW, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,12,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Eguchi, N., Vaziri, N.D., Dafoe, D.C., and Ichii, H. (2021). The role of oxidative stress in pancreatic \u03b2 cell dysfunction in diabetes. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22041509"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Ben\u00e1kov\u00e1, \u0160., Holendov\u00e1, B., and Plecit\u00e1-Hlavat\u00e1, L. (2021). Redox homeostasis in pancreatic \u03b2-cells: From development to failure. Antioxidants, 10.","DOI":"10.3390\/antiox10040526"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1089\/ars.2016.6755","article-title":"The Role of Oxidative Stress and Hypoxia in Pancreatic Beta-Cell Dysfunction in Diabetes Mellitus","volume":"26","author":"Gerber","year":"2017","journal-title":"Antioxid. Redox Signal."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1016\/j.cub.2014.03.034","article-title":"ROS function in redox signaling","volume":"24","author":"Schieber","year":"2014","journal-title":"Curr. Biol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1111\/apha.12256","article-title":"Iron: The hard player in diabetes pathophysiology","volume":"210","author":"Hansen","year":"2014","journal-title":"Acta Physiol."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Blesia, V., Patel, V.B., Al-Obaidi, H., Renshaw, D., and Zariwala, M.G. (2021). Excessive Iron Induces Oxidative Stress Promoting Cellular Perturbations and Insulin Secretory Dysfunction in MIN6 Beta Cells. Cells, 10.","DOI":"10.3390\/cells10051141"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Tran, N., Pham, B., and Le, L. (2020). Bioactive compounds in anti-diabetic plants: From herbal medicine to modern drug discovery. Biology, 9.","DOI":"10.3390\/biology9090252"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1858","DOI":"10.1002\/tcr.201800094","article-title":"Valorization of Food and Agricultural Waste: A Step towards Greener Future","volume":"19","author":"Rao","year":"2019","journal-title":"Chem. Rec."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Schiano, E., Piccolo, V., Novellino, E., Maisto, M., Iannuzzo, F., Summa, V., and Tenore, G.C. (2022). Thinned Nectarines, an Agro-Food Waste with Antidiabetic Potential: HPLC-HESI-MS\/MS Phenolic Characterization and In Vitro Evaluation of Their Beneficial Activities. Foods, 11.","DOI":"10.3390\/foods11071010"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"902","DOI":"10.1002\/jsfa.7813","article-title":"Thinned stone fruits are a source of polyphenols and antioxidant compounds","volume":"97","author":"Redondo","year":"2017","journal-title":"J. Sci. Food Agric."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"4577","DOI":"10.1093\/jxb\/eru204","article-title":"The role of abscisic acid in fruit ripening and responses to abiotic stress","volume":"65","author":"Leng","year":"2014","journal-title":"J. Exp. Bot."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"24","DOI":"10.3389\/fnut.2017.00024","article-title":"Abscisic Acid: A Novel Nutraceutical for Glycemic Control","volume":"4","author":"Sordillo","year":"2017","journal-title":"Front. Nutr."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Magnone, M., Sturla, L., Guida, L., Spinelli, S., Begani, G., Bruzzone, S., Fresia, C., and Zocchi, E. (2020). Abscisic acid: A conserved hormone in plants and humans and a promising aid to combat prediabetes and the metabolic syndrome. Nutrients, 12.","DOI":"10.3390\/nu12061724"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1067","DOI":"10.2174\/1568026621666210509161059","article-title":"Bioactive Compounds Effective Against Type 2 Diabetes Mellitus: A Systematic Review","volume":"21","author":"Egbuna","year":"2021","journal-title":"Curr. Top. Med. Chem."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1002\/jbt.10058","article-title":"Diabetes, oxidative stress, and antioxidants: A review","volume":"17","author":"Maritim","year":"2003","journal-title":"J. Biochem. Mol. Toxicol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1272","DOI":"10.1080\/10942912.2020.1797782","article-title":"Antioxidant profile of thinned young and ripe fruits of Chinese peach and nectarine varieties","volume":"23","author":"Guo","year":"2020","journal-title":"Int. J. Food Prop."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1007\/s10681-009-0023-4","article-title":"Phenotypic diversity and relationships of fruit quality traits in peach and nectarine [Prunus persica (L.) Batsch] breeding progenies","volume":"171","author":"Gogorcena","year":"2010","journal-title":"Euphytica"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2155","DOI":"10.1007\/s11947-022-02807-2","article-title":"Nanonutraceuticals\u2014Challenges and Novel Nano-based Carriers for Effective Delivery and Enhanced Bioavailability","volume":"15","author":"Muthukrishnan","year":"2022","journal-title":"Food Bioprocess Technol."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Mursaleen, L., Chan, S.H.Y., Noble, B., Somavarapu, S., and Zariwala, M.G. (2023). Curcumin and N-Acetylcysteine Nanocarriers Alone or Combined with Deferoxamine Target the Mitochondria and Protect against Neurotoxicity and Oxidative Stress in a Co-Culture Model of Parkinson\u2019s Disease. Antioxidants, 12.","DOI":"10.3390\/antiox12010130"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Mursaleen, L., Noble, B., Somavarapu, S., and Zariwala, M.G. (2021). Micellar nanocarriers of hydroxytyrosol are protective against parkinson\u2019s related oxidative stress in an in vitro hcmec\/d3-sh-sy5y co-culture system. Antioxidants, 10.","DOI":"10.3390\/antiox10060887"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.colsurfb.2013.11.028","article-title":"Ascorbyl palmitate\/DSPE-PEG nanocarriers for oral iron delivery: Preparation, characterisation and in vitro evaluation","volume":"115","author":"Zariwala","year":"2014","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"138929","DOI":"10.1155\/2011\/138929","article-title":"Drug Nanoparticle Formulation Using Ascorbic Acid Derivatives","volume":"2011","author":"Moribe","year":"2011","journal-title":"J. Drug Deliv."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Schiano, E., Neri, I., Maisto, M., Novellino, E., Iannuzzo, F., Piccolo, V., Summa, V., Grumetto, L., and Tenore, G.C. (2023). Validation of an LC-MS\/MS Method for the Determination of Abscisic Acid Concentration in a Real-World Setting. Foods, 12.","DOI":"10.3390\/foods12051077"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"10940","DOI":"10.1021\/jf4034305","article-title":"Development of a rapid LC-DAD\/FLD method for the simultaneous determination of auxins and abscisic acid in plant extracts","volume":"61","author":"Bosco","year":"2013","journal-title":"J. Agric. Food Chem."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"8896","DOI":"10.1021\/jf0715166","article-title":"Cellular antioxidant activity (CAA) assay for assessing antioxidants, foods, and dietary supplements","volume":"55","author":"Wolfe","year":"2007","journal-title":"J. Agric. Food Chem."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Pap, R., Pandur, E., J\u00e1nosa, G., Sipos, K., Fritz, F.R., Nagy, T., Ag\u00f3cs, A., and Deli, J. (2023). Protective Effects of 3\u2032-Epilutein and 3\u2032-Oxolutein against Glutamate-Induced Neuronal Damage. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms241512008"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1152\/physiologyonline.2000.15.2.72","article-title":"The Cell Physiology of Biphasic Insulin Secretion","volume":"15","author":"Rorsman","year":"2000","journal-title":"Physiology"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"32188","DOI":"10.1074\/jbc.M802603200","article-title":"Abscisic acid is an endogenous stimulator of insulin release from human pancreatic islets with cyclic ADP ribose as second messenger","volume":"283","author":"Bruzzone","year":"2008","journal-title":"J. Biol. Chem."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"11","DOI":"10.4103\/1110-2098.178938","article-title":"Role of iron in diabetes mellitus and its complications","volume":"29","author":"Shaaban","year":"2016","journal-title":"Menoufia Med. J."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Tanabe, K., Liu, Y., Hasan, S.D., Martinez, S.C., Cras-M\u00e9neur, C., Welling, C.M., Bernal-Mizrachi, E., Tanizawa, Y., Rhodes, C.J., and Zmuda, E. (2011). Glucose and fatty acids synergize to promote B-cell apoptosis through activation of glycogen synthase kinase 3\u03b2 independent of JNK activation. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0018146"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"703538","DOI":"10.1155\/2012\/703538","article-title":"Mitochondrial Dysfunction and \u03b2-Cell Failure in Type 2 Diabetes Mellitus","volume":"2012","author":"Ma","year":"2012","journal-title":"Exp. Diabetes Res."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Marku, A., Galli, A., Marciani, P., Dule, N., Perego, C., and Castagna, M. (2021). Iron Metabolism in Pancreatic Beta-Cell Function and Dysfunction. Cells, 10.","DOI":"10.3390\/cells10112841"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1398","DOI":"10.1016\/j.bbagen.2019.06.010","article-title":"Iron homeostasis and iron-regulated ROS in cell death, senescence and human diseases","volume":"1863","author":"Nakamura","year":"2019","journal-title":"Biochim. Biophys. Acta-Gen. Subj."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.tips.2010.11.006","article-title":"Reactive oxygen species and insulin resistance: The good, the bad and the ugly","volume":"32","author":"Tiganis","year":"2011","journal-title":"Trends Pharmacol. Sci."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1530\/EJE-13-0145","article-title":"Body iron stores and the risk of type 2 diabetes in middle-aged men","volume":"169","author":"Aregbesola","year":"2013","journal-title":"Eur. J. Endocrinol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"2043","DOI":"10.3390\/foods10092043","article-title":"Comprehensive Utilization of Thinned Unripe Fruits from Horticultural Crops","volume":"10","author":"Mengyuan","year":"2021","journal-title":"Foods"},{"key":"ref_37","first-page":"182","article-title":"Emerging nanoformulation strategies for phytocompounds and applications from drug delivery to phototherapy to imaging","volume":"14","author":"Han","year":"2021","journal-title":"Bioact. Mater."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Bala, S., Garg, D., Sridhar, K., Inbaraj, B.S., Singh, R., Kamma, S., Tripathi, M., and Sharma, M. (2023). Transformation of Agro-Waste into Value-Added Bioproducts and Bioactive Compounds: Micro\/Nano Formulations and Application in the Agri-Food-Pharma Sector. Bioengineering, 10.","DOI":"10.3390\/bioengineering10020152"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/j.addr.2019.04.008","article-title":"Concepts of nanoparticle cellular uptake, intracellular trafficking, and kinetics in nanomedicine","volume":"143","author":"Donahue","year":"2019","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.copbio.2017.01.002","article-title":"Advances in the clinical translation of nanotechnology","volume":"46","author":"Scheinberg","year":"2017","journal-title":"Curr. Opin. Biotechnol."}],"container-title":["Antioxidants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-3921\/13\/1\/63\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T21:45:13Z","timestamp":1760132713000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-3921\/13\/1\/63"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,12,31]]},"references-count":40,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2024,1]]}},"alternative-id":["antiox13010063"],"URL":"https:\/\/doi.org\/10.3390\/antiox13010063","relation":{},"ISSN":["2076-3921"],"issn-type":[{"type":"electronic","value":"2076-3921"}],"subject":[],"published":{"date-parts":[[2023,12,31]]}}}