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The cerebellum is highly vulnerable to oxidative stress and thus, obesogenic diets might be particularly detrimental to this tissue. However, the precise molecular mechanisms behind obesity-related brain damage are still not clear. Since protein carbonylation, a biomarker of oxidative stress, influences protein functions and is involved in metabolic control, the current investigation addressed the effect of long-term high-fat and high-sucrose diet intake on the cerebellum of Sprague-Dawley rats by deciphering the changes caused in the carbonylated proteome. The antioxidant effects of fish oil supplementation on cerebellar carbonylated proteins were also investigated. Lipid peroxidation products and carbonylated proteins were identified and quantified using immunoassays and 2D-LC-MS\/MS in the cerebellum. After 21 weeks of nutritional intervention, the obesogenic diet selectively increased carbonylation of the proteins that participate in ATP homeostasis and glutamate metabolism in the cerebellum. Moreover, the data demonstrated that fish oil supplementation restrained carbonylation of the main protein targets oxidatively damaged by the obesogenic diet, and additionally protected against carbonylation of several other proteins involved in amino acid biosynthesis and neurotransmission. Therefore, dietary interventions with fish oils could help the cerebellum to be more resilient to oxidative damage. The results could shed some light on the effect of high-fat and high-sucrose diets on redox homeostasis in the cerebellum and boost the development of antioxidant-based nutritional interventions to improve cerebellum health.<\/jats:p>","DOI":"10.3390\/antiox13010103","type":"journal-article","created":{"date-parts":[[2024,1,15]],"date-time":"2024-01-15T04:03:26Z","timestamp":1705291406000},"page":"103","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Dietary Marine Oils Selectively Decrease Obesogenic Diet-Derived Carbonylation in Proteins Involved in ATP Homeostasis and Glutamate Metabolism in the Rat Cerebellum"],"prefix":"10.3390","volume":"13","author":[{"given":"Francisco","family":"Moreno","sequence":"first","affiliation":[{"name":"Instituto de Investigaciones Marinas\u2014Consejo Superior de Investigaciones Cient\u00edficas (IIM-CSIC), Eduardo Cabello 6, E-36208 Vigo, Spain"},{"name":"Universidad de Vigo, Circunvalaci\u00f3n ao Campus Universitario, E-36310 Vigo, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9711-2994","authenticated-orcid":false,"given":"Luc\u00eda","family":"M\u00e9ndez","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Marinas\u2014Consejo Superior de Investigaciones Cient\u00edficas (IIM-CSIC), Eduardo Cabello 6, E-36208 Vigo, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-8395-7276","authenticated-orcid":false,"given":"Ana","family":"Raner","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Marinas\u2014Consejo Superior de Investigaciones Cient\u00edficas (IIM-CSIC), Eduardo Cabello 6, E-36208 Vigo, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1294-7069","authenticated-orcid":false,"given":"Bernat","family":"Miralles-P\u00e9rez","sequence":"additional","affiliation":[{"name":"Unidad de Farmacolog\u00eda, Facultad de Medicina y Ciencias de la Salud, Universidad Rovira i Virgili, Sant Lloren\u00e7 21, E-43201 Reus, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2131-1858","authenticated-orcid":false,"given":"Marta","family":"Romeu","sequence":"additional","affiliation":[{"name":"Unidad de Farmacolog\u00eda, Facultad de Medicina y Ciencias de la Salud, Universidad Rovira i Virgili, Sant Lloren\u00e7 21, E-43201 Reus, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9293-4454","authenticated-orcid":false,"given":"Sara","family":"Ramos-Romero","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Physiology and Immunology, Faculty of Biology, University of Barcelona, Av Diagonal 643, E-08028 Barcelona, Spain"},{"name":"Nutrition & Food Safety Research Institute (INSA-UB), Maria de Maeztu Unit of Excellence, E-08921 Santa Coloma de Gramenet, Spain"},{"name":"Instituto de Qu\u00edmica Avanzada de Catalunya\u2014Consejo Superior de Investigaciones Cient\u00edficas (IQAC-CSIC), Jordi Girona 18-26, E-08034 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Josep Llu\u00eds","family":"Torres","sequence":"additional","affiliation":[{"name":"Nutrition & Food Safety Research Institute (INSA-UB), Maria de Maeztu Unit of Excellence, E-08921 Santa Coloma de Gramenet, Spain"},{"name":"Instituto de Qu\u00edmica Avanzada de Catalunya\u2014Consejo Superior de Investigaciones Cient\u00edficas (IQAC-CSIC), Jordi Girona 18-26, E-08034 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isabel","family":"Medina","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Marinas\u2014Consejo Superior de Investigaciones Cient\u00edficas (IIM-CSIC), Eduardo Cabello 6, E-36208 Vigo, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,1,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"564413","DOI":"10.3389\/fpsyg.2020.564413","article-title":"Western Diet: Implications for Brain Function and Behavior","volume":"11","author":"Conejo","year":"2020","journal-title":"Front. 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