{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T19:28:24Z","timestamp":1789759704106,"version":"4.0.1"},"reference-count":83,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2024,1,29]],"date-time":"2024-01-29T00:00:00Z","timestamp":1706486400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"European Union\u2019s Regional Development Fund (EFRE)","award":["IMPACT 674911"],"award-info":[{"award-number":["IMPACT 674911"]}]},{"name":"European Union\u2019s Regional Development Fund (EFRE)","award":["BMBF 01DQ16010"],"award-info":[{"award-number":["BMBF 01DQ16010"]}]},{"name":"H2020-EU-ITN-IMPACT Marie Sk\u0142odowska-Curie grant","award":["IMPACT 674911"],"award-info":[{"award-number":["IMPACT 674911"]}]},{"name":"H2020-EU-ITN-IMPACT Marie Sk\u0142odowska-Curie grant","award":["BMBF 01DQ16010"],"award-info":[{"award-number":["BMBF 01DQ16010"]}]},{"name":"Inno-INDIGO-NCDs-CAPomics Project","award":["IMPACT 674911"],"award-info":[{"award-number":["IMPACT 674911"]}]},{"name":"Inno-INDIGO-NCDs-CAPomics Project","award":["BMBF 01DQ16010"],"award-info":[{"award-number":["BMBF 01DQ16010"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>Due to their immediate exhalation after generation at the cellular\/microbiome levels, exhaled volatile organic compounds (VOCs) may provide real-time information on pathophysiological mechanisms and the host response to infection. In recent years, the metabolic profiling of the most frequent respiratory infections has gained interest as it holds potential for the early, non-invasive detection of pathogens and the monitoring of disease progression and the response to therapy. Using previously unpublished data, randomly selected individuals from a COVID-19 test center were included in the study. Based on multiplex PCR results (non-SARS-CoV-2 respiratory pathogens), the breath profiles of 479 subjects with the presence or absence of flu-like symptoms were obtained using proton-transfer-reaction time-of-flight mass spectrometry. Among 223 individuals, one respiratory pathogen was detected in 171 cases, and more than one pathogen in 52 cases. A total of 256 subjects had negative PCR test results and had no symptoms. The exhaled VOC profiles were affected by the presence of Haemophilus influenzae, Streptococcus pneumoniae, and Rhinovirus. The endogenous ketone, short-chain fatty acid, organosulfur, aldehyde, and terpene concentrations changed, but only a few compounds exhibited concentration changes above inter-individual physiological variations. Based on the VOC origins, the observed concentration changes may be attributed to oxidative stress and antioxidative defense, energy metabolism, systemic microbial immune homeostasis, and inflammation. In contrast to previous studies with pre-selected patient groups, the results of this study demonstrate the broad inter-individual variations in VOC profiles in real-life screening conditions. As no unique infection markers exist, only concentration changes clearly above the mentioned variations can be regarded as indicative of infection or colonization.<\/jats:p>","DOI":"10.3390\/antiox13020172","type":"journal-article","created":{"date-parts":[[2024,1,29]],"date-time":"2024-01-29T08:11:27Z","timestamp":1706515887000},"page":"172","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Effects of Contagious Respiratory Pathogens on Breath Biomarkers"],"prefix":"10.3390","volume":"13","author":[{"given":"Nele","family":"Kemnitz","sequence":"first","affiliation":[{"name":"Rostock Medical Breath Research Analytics and Technologies (ROMBAT), Department of Anaesthesiology, Intensive Care Medicine and Pain Therapy, University Medicine Rostock, 18057 Rostock, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0055-6098","authenticated-orcid":false,"given":"Patricia","family":"Fuchs","sequence":"additional","affiliation":[{"name":"Rostock Medical Breath Research Analytics and Technologies (ROMBAT), Department of Anaesthesiology, Intensive Care Medicine and Pain Therapy, University Medicine Rostock, 18057 Rostock, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rasmus","family":"Remy","sequence":"additional","affiliation":[{"name":"Rostock Medical Breath Research Analytics and Technologies (ROMBAT), Department of Anaesthesiology, Intensive Care Medicine and Pain Therapy, University Medicine Rostock, 18057 Rostock, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0971-5120","authenticated-orcid":false,"given":"Leo","family":"Ruehrmund","sequence":"additional","affiliation":[{"name":"Rostock Medical Breath Research Analytics and Technologies (ROMBAT), Department of Anaesthesiology, Intensive Care Medicine and Pain Therapy, University Medicine Rostock, 18057 Rostock, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Bartels","sequence":"additional","affiliation":[{"name":"Rostock Medical Breath Research Analytics and Technologies (ROMBAT), Department of Anaesthesiology, Intensive Care Medicine and Pain Therapy, University Medicine Rostock, 18057 Rostock, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0097-6321","authenticated-orcid":false,"given":"Ann-Christin","family":"Klemenz","sequence":"additional","affiliation":[{"name":"Rostock Medical Breath Research Analytics and Technologies (ROMBAT), Department of Anaesthesiology, Intensive Care Medicine and Pain Therapy, University Medicine Rostock, 18057 Rostock, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phillip","family":"Trefz","sequence":"additional","affiliation":[{"name":"Rostock Medical Breath Research Analytics and Technologies (ROMBAT), Department of Anaesthesiology, Intensive Care Medicine and Pain Therapy, University Medicine Rostock, 18057 Rostock, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1619-0315","authenticated-orcid":false,"given":"Wolfram","family":"Miekisch","sequence":"additional","affiliation":[{"name":"Rostock Medical Breath Research Analytics and Technologies (ROMBAT), Department of Anaesthesiology, Intensive Care Medicine and Pain Therapy, University Medicine Rostock, 18057 Rostock, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jochen K.","family":"Schubert","sequence":"additional","affiliation":[{"name":"Rostock Medical Breath Research Analytics and Technologies (ROMBAT), Department of Anaesthesiology, Intensive Care Medicine and Pain Therapy, University Medicine Rostock, 18057 Rostock, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5114-1776","authenticated-orcid":false,"given":"Pritam","family":"Sukul","sequence":"additional","affiliation":[{"name":"Rostock Medical Breath Research Analytics and Technologies (ROMBAT), Department of Anaesthesiology, Intensive Care Medicine and Pain Therapy, University Medicine Rostock, 18057 Rostock, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,1,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1038\/s41572-021-00259-0","article-title":"Pneumonia","volume":"7","author":"Torres","year":"2021","journal-title":"Nat. Rev. Dis. Prim."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1045","DOI":"10.1093\/cid\/cix941","article-title":"Dynamics of Bacterial Colonization with Streptococcus Pneumoniae, Haemophilus Influenzae, and Moraxella Catarrhalis During Symptomatic and Asymptomatic Viral Upper Respiratory Tract Infection","volume":"66","author":"DeMuri","year":"2018","journal-title":"Clin. Infect. Dis. Off. Publ. Infect. Dis. Soc. Am."},{"key":"ref_3","first-page":"1","article-title":"The Effects of the COVID-19 Pandemic on Community Respiratory Virus Activity","volume":"21","author":"Chow","year":"2022","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1038\/s41579-022-00821-x","article-title":"The Dynamic Lung Microbiome in Health and Disease","volume":"21","author":"Natalini","year":"2022","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/j.chom.2009.07.006","article-title":"Gene Expression Signatures Diagnose Influenza and Other Symptomatic Respiratory Viral Infections in Humans","volume":"6","author":"Zaas","year":"2009","journal-title":"Cell Host Microbe"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1016\/j.molmed.2014.08.001","article-title":"The Current Epidemiology and Clinical Decisions Surrounding Acute Respiratory Infections","volume":"20","author":"Zaas","year":"2014","journal-title":"Trends Mol. Med."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"13562","DOI":"10.1073\/pnas.0804181105","article-title":"Rhinovirus-Induced Lower Respiratory Illness Is Increased in Asthma and Related to Virus Load and Th1\/2 Cytokine and IL-10 Production","volume":"105","author":"Message","year":"2008","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1016\/j.cell.2020.05.042","article-title":"SARS-CoV-2 Reverse Genetics Reveals a Variable Infection Gradient in the Respiratory Tract","volume":"182","author":"Hou","year":"2020","journal-title":"Cell"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"18894","DOI":"10.1038\/s41598-019-55334-0","article-title":"Volatile Scents of Influenza A and S. Pyogenes (Co-)Infected Cells","volume":"9","author":"Traxler","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"14857","DOI":"10.1038\/s41598-018-33061-2","article-title":"VOC Breath Profile in Spontaneously Breathing Awake Swine during Influenza A Infection","volume":"8","author":"Traxler","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"108062","DOI":"10.1016\/j.celrep.2020.108062","article-title":"Influenza-Induced Oxidative Stress Sensitizes Lung Cells to Bacterial-Toxin-Mediated Necroptosis","volume":"32","author":"Riegler","year":"2020","journal-title":"Cell Rep."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"322ra11","DOI":"10.1126\/scitranslmed.aad6873","article-title":"Host Gene Expression Classifiers Diagnose Acute Respiratory Illness Etiology","volume":"8","author":"Tsalik","year":"2016","journal-title":"Sci. Transl. Med."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"105195","DOI":"10.1016\/j.isci.2022.105195","article-title":"Profiling of Exhaled Volatile Organics in the Screening Scenario of a COVID-19 Test Center","volume":"25","author":"Remy","year":"2022","journal-title":"iScience"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"eabq5126","DOI":"10.1126\/scitranslmed.abq5126","article-title":"Understanding Respiratory Microbiome\u2013Immune System Interactions in Health and Disease","volume":"15","author":"Rudloff","year":"2023","journal-title":"Sci. Transl. Med."},{"key":"ref_15","unstructured":"Miekisch, W., Sukul, P., and Schubert, J.K. (2023). Volatile Biomarkers for Human Health: From Nature to Artificial Senses, The Royal Society of Chemistry."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2200009","DOI":"10.1183\/13993003.00009-2022","article-title":"Effects of COVID-19 Protective Face-Masks and Wearing Durations onto Respiratory-Haemodynamic Physiology and Exhaled Breath Constituents","volume":"60","author":"Sukul","year":"2022","journal-title":"Eur. Respir. J."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"14109","DOI":"10.1038\/s41598-020-70993-0","article-title":"Exhaled Breath Compositions under Varying Respiratory Rhythms Reflects Ventilatory Variations: Translating Breathomics towards Respiratory Medicine","volume":"10","author":"Sukul","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"28029","DOI":"10.1038\/srep28029","article-title":"FEV Manoeuvre Induced Changes in Breath VOC Compositions: An Unconventional View on Lung Function Tests","volume":"6","author":"Sukul","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"047105","DOI":"10.1088\/1752-7155\/9\/4\/047105","article-title":"Instant Effects of Changing Body Positions on Compositions of Exhaled Breath","volume":"9","author":"Sukul","year":"2015","journal-title":"J. Breath Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/11663_2022_19","article-title":"Physio-Metabolic Monitoring via Breath Employing Real-Time Mass Spectrometry: Importance, Challenges, Potentials, and Pitfalls","volume":"Volume 4","author":"Sukul","year":"2022","journal-title":"Bioanalytical Reviews"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"103739","DOI":"10.1016\/j.isci.2022.103739","article-title":"Physiological and Metabolic Effects of Healthy Female Aging on Exhaled Breath Biomarkers","volume":"25","author":"Sukul","year":"2022","journal-title":"iScience"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"10838","DOI":"10.1038\/s41598-018-29221-z","article-title":"Natural Menstrual Rhythm and Oral Contraception Diversely Affect Exhaled Breath Compositions","volume":"8","author":"Sukul","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Trefz, P., Schmidt, S.C., Sukul, P., Schubert, J.K., Miekisch, W., and Fischer, D.-C. (2019). Non-Invasive Assessment of Metabolic Adaptation in Paediatric Patients Suffering from Type 1 Diabetes Mellitus. J. Clin. Med., 8.","DOI":"10.3390\/jcm8111797"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"e05922","DOI":"10.1016\/j.heliyon.2021.e05922","article-title":"Deficiency and Absence of Endogenous Isoprene in Adults, Disqualified Its Putative Origin","volume":"7","author":"Sukul","year":"2021","journal-title":"Heliyon"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"946401","DOI":"10.3389\/fphys.2022.946401","article-title":"Real-Time Metabolic Monitoring under Exhaustive Exercise and Evaluation of Ventilatory Threshold by Breathomics: Independent Validation of Evidence and Advances","volume":"13","author":"Pugliese","year":"2022","journal-title":"Front. Physiol."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Sukul, P., Richter, A., Junghanss, C., Schubert, J.K., and Miekisch, W. (2023). Origin of Breath Isoprene in Humans Is Revealed via Multi-Omic Investigations. Commun. Biol., 6.","DOI":"10.1038\/s42003-023-05384-y"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"L\u00f6ser, B., Grabenschr\u00f6er, A., Pugliese, G., Sukul, P., Trefz, P., Schubert, J.K., and Miekisch, W. (2020). Changes of Exhaled Volatile Organic Compounds in Postoperative Patients Undergoing Analgesic Treatment: A Prospective Observational Study. Metabolites, 10.","DOI":"10.3390\/metabo10080321"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"016005","DOI":"10.1088\/1752-7163\/aa53b2","article-title":"Monitoring of Breath VOCs and Electrical Impedance Tomography under Pulmonary Recruitment in Mechanically Ventilated Patients","volume":"11","author":"Brock","year":"2017","journal-title":"J. Breath Res."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Grassin-Delyle, S., Roquencourt, C., Moine, P., Saffroy, G., Carn, S., Heming, N., Fleuriet, J., Salvator, H., Naline, E., and Couderc, L.-J. (2021). Metabolomics of Exhaled Breath in Critically Ill COVID-19 Patients: A Pilot Study. EBioMedicine, 63.","DOI":"10.1016\/j.ebiom.2020.103154"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"17926","DOI":"10.1038\/s41598-022-22581-7","article-title":"Advanced Setup for Safe Breath Sampling and Patient Monitoring under Highly Infectious Conditions in the Clinical Environment","volume":"12","author":"Sukul","year":"2022","journal-title":"Sci. Rep."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"00139-2021","DOI":"10.1183\/23120541.00139-2021","article-title":"Diagnosis of COVID-19 by Exhaled Breath Analysis Using Gas Chromatography\u2013Mass Spectrometry","volume":"7","author":"Ibrahim","year":"2021","journal-title":"ERJ Open Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"100609","DOI":"10.1016\/j.eclinm.2020.100609","article-title":"Diagnosis of COVID-19 by Analysis of Breath with Gas Chromatography-Ion Mobility Spectrometry\u2014A Feasibility Study","volume":"29","author":"Ruszkiewicz","year":"2020","journal-title":"EclinicalMedicine"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"2079","DOI":"10.1007\/s00216-011-5173-2","article-title":"Phase-Resolved Real-Time Breath Analysis during Exercise by Means of Smart Processing of PTR-MS Data","volume":"401","author":"Schwoebel","year":"2011","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"2283","DOI":"10.1021\/es203985t","article-title":"On Quantitative Determination of Volatile Organic Compound Concentrations Using Proton Transfer Reaction Time-of-Flight Mass Spectrometry","volume":"46","author":"Cappellin","year":"2012","journal-title":"Environ. Sci. Technol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"026016","DOI":"10.1088\/1752-7163\/aa9eea","article-title":"Effects of Humidity, CO2 and O2 on Real-Time Quantitation of Breath Biomarkers by Means of PTR-ToF-MS","volume":"12","author":"Trefz","year":"2018","journal-title":"J. Breath Res."},{"key":"ref_36","unstructured":"Preedy, V.R. (2020). Pathology, Academic Press."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1097\/WOX.0b013e3182439613","article-title":"Oxidative Stress and Antioxidant Defense","volume":"5","author":"Birben","year":"2012","journal-title":"World Allergy Organ. J."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"21837","DOI":"10.1074\/jbc.R700019200","article-title":"Oxidative Stress and Covalent Modification of Protein with Bioactive Aldehydes","volume":"283","author":"Grimsrud","year":"2008","journal-title":"J. Biol. Chem."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"4123","DOI":"10.1099\/mic.0.028282-0","article-title":"The Pneumococcal Response to Oxidative Stress Includes a Role for Rgg","volume":"155","author":"Bortoni","year":"2009","journal-title":"Microbiology"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1822","DOI":"10.1093\/infdis\/jiu806","article-title":"Streptococcus Pneumoniae-Induced Oxidative Stress in Lung Epithelial Cells Depends on Pneumococcal Autolysis and Is Reversible by Resveratrol","volume":"211","author":"Zahlten","year":"2015","journal-title":"J. Infect. Dis."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/j.tim.2013.01.004","article-title":"Streptococcus Pneumoniae and Reactive Oxygen Species: An Unusual Approach to Living with Radicals","volume":"21","author":"Yesilkaya","year":"2013","journal-title":"Trends Microbiol."},{"key":"ref_42","first-page":"PA3989","article-title":"Effect of Oxidative Stress and Rhinovirus Infection on Mitochondrial\/Endoplasmic Reticular Function in Human Primary Bronchial Epithelial Cells","volume":"48","author":"Wark","year":"2016","journal-title":"Eur. Respir. J."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"454","DOI":"10.1016\/S0891-5849(98)00233-0","article-title":"The Role of Oxidative Stress in Rhinovirus Induced Elaboration of IL-8 by Respiratory Epithelial Cells","volume":"26","author":"Biagioli","year":"1999","journal-title":"Free Radic. Biol. Med."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1885","DOI":"10.1086\/315504","article-title":"Rhinovirus-Induced Oxidative Stress and Interleukin-8 Elaboration Involves P47-Phox but Is Independent of Attachment to Intercellular Adhesion Molecule-1 and Viral Replication","volume":"181","author":"Kaul","year":"2000","journal-title":"J. Infect. Dis."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.phymed.2018.09.027","article-title":"Gastroprotective Effect of Limonene in Rats: Influence on Oxidative Stress, Inflammation and Gene Expression","volume":"53","author":"Vieira","year":"2019","journal-title":"Phytomedicine"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"2182","DOI":"10.2174\/1381612826666200320130443","article-title":"Anti-Inflammatory Activity of Limonene in the Prevention and Control of Injuries in the Respiratory System: A Systematic Review","volume":"26","author":"Santana","year":"2020","journal-title":"Curr. Pharm. Des."},{"key":"ref_47","unstructured":"Bacanl\u0131, M. (2020). Diabetes, Academic Press."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1016\/S0304-4165(03)00051-5","article-title":"On the Mammalian Acetone Metabolism: From Chemistry to Clinical Implications","volume":"1621","author":"Kalapos","year":"2003","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Othman, D.S.M.P., Schirra, H., McEwan, A.G., and Kappler, U. (2014). Metabolic Versatility in Haemophilus Influenzae: A Metabolomic and Genomic Analysis. Front. Microbiol., 5.","DOI":"10.3389\/fmicb.2014.00069"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"624775","DOI":"10.3389\/fimmu.2021.624775","article-title":"Glycolytic Metabolism Is Critical for the Innate Antibacterial Defense in Acute Streptococcus Pneumoniae Otitis Media","volume":"12","author":"Fan","year":"2021","journal-title":"Front. Immunol."},{"key":"ref_51","unstructured":"(2023, February 01). Bacterial Growth Laws Reflect the Evolutionary Importance of Energy Efficiency\u2014PMC, Available online: https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC4299221\/."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1135","DOI":"10.1038\/2021135b0","article-title":"Energy Requirement for Microbial Growth","volume":"202","author":"Chen","year":"1964","journal-title":"Nature"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1186\/s40348-016-0049-3","article-title":"Mechanism of Human Rhinovirus Infections","volume":"3","author":"Blaas","year":"2016","journal-title":"Mol. Cell. Pediatr."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1007\/s11882-016-0608-7","article-title":"Rhinoviruses and Their Receptors: Implications for Allergic Disease","volume":"16","author":"Bochkov","year":"2016","journal-title":"Curr. Allergy Asthma Rep."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"E7158","DOI":"10.1073\/pnas.1800525115","article-title":"Rhinovirus Induces an Anabolic Reprogramming in Host Cell Metabolism Essential for Viral Replication","volume":"115","author":"Gualdoni","year":"2018","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Cope, E.K., Goldstein-Daruech, N., Kofonow, J.M., Christensen, L., McDermott, B., Monroy, F., Palmer, J.N., Chiu, A.G., Shirtliff, M.E., and Cohen, N.A. (2011). Regulation of Virulence Gene Expression Resulting from Streptococcus Pneumoniae and Nontypeable Haemophilus Influenzae Interactions in Chronic Disease. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0028523"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1111\/2049-632X.12073","article-title":"Haemophilus Influenzae and Streptococcus Pneumoniae: Living Together in a Biofilm","volume":"69","author":"Tikhomirova","year":"2013","journal-title":"Pathog. Dis."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"3990","DOI":"10.1128\/IAI.68.7.3990-3997.2000","article-title":"Inhibitory and Bactericidal Effects of Hydrogen Peroxide Production by Streptococcus Pneumoniae on Other Inhabitants of the Upper Respiratory Tract","volume":"68","author":"Pericone","year":"2000","journal-title":"Infect. Immun."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"7161","DOI":"10.1128\/IAI.70.12.7161-7164.2002","article-title":"Neuraminidase Expressed by Streptococcus Pneumoniae Desialylates the Lipopolysaccharide of Neisseria Meningitidis and Haemophilus Influenzae: A Paradigm for Interbacterial Competition among Pathogens of the Human Respiratory Tract","volume":"70","author":"Shakhnovich","year":"2002","journal-title":"Infect. Immun."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1128\/IAI.72.1.106-113.2004","article-title":"Sialylation of Lipooligosaccharides Promotes Biofilm Formation by Nontypeable Haemophilus Influenzae","volume":"72","author":"Swords","year":"2004","journal-title":"Infect. Immun."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"25","DOI":"10.3389\/fendo.2020.00025","article-title":"The Role of Short-Chain Fatty Acids from Gut Microbiota in Gut-Brain Communication","volume":"11","author":"Silva","year":"2020","journal-title":"Front. Endocrinol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"103953","DOI":"10.1016\/j.jff.2020.103953","article-title":"Acetic Acid and Butyric Acid Released in Large Intestine Play Different Roles in the Alleviation of Constipation","volume":"69","author":"Wang","year":"2020","journal-title":"J. Funct. Foods"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"3366","DOI":"10.1016\/j.jchromb.2009.05.026","article-title":"Measurement and Biological Significance of the Volatile Sulfur Compounds Hydrogen Sulfide, Methanethiol and Dimethyl Sulfide in Various Biological Matrices","volume":"877","author":"Tangerman","year":"2009","journal-title":"J. Chromatogr. B"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"613","DOI":"10.3389\/fgene.2019.00613","article-title":"Gut Microbiota Composition Is Associated with the Global DNA Methylation Pattern in Obesity","volume":"10","author":"Tinahones","year":"2019","journal-title":"Front. Genet."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"10493","DOI":"10.1074\/jbc.REV120.011188","article-title":"Oxygen Battle in the Gut: Hypoxia and Hypoxia-Inducible Factors in Metabolic and Inflammatory Responses in the Intestine","volume":"295","author":"Singhal","year":"2020","journal-title":"J. Biol. Chem."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1016\/j.chom.2022.02.004","article-title":"Gut Microbiota-Derived Bile Acids in Intestinal Immunity, Inflammation, and Tumorigenesis","volume":"30","author":"Cai","year":"2022","journal-title":"Cell Host Microbe"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"844335","DOI":"10.3389\/fimmu.2022.844335","article-title":"Effects of Gut Microbiota on Host Adaptive Immunity Under Immune Homeostasis and Tumor Pathology State","volume":"13","author":"Li","year":"2022","journal-title":"Front. Immunol."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/j.chom.2020.07.004","article-title":"Early Life Microbiota and Respiratory Tract Infections","volume":"28","author":"Piters","year":"2020","journal-title":"Cell Host Microbe"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"2934","DOI":"10.1016\/j.celrep.2020.02.013","article-title":"Gut Dysbiosis during Influenza Contributes to Pulmonary Pneumococcal Superinfection through Altered Short-Chain Fatty Acid Production","volume":"30","author":"Sencio","year":"2020","journal-title":"Cell Rep."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1038\/s43856-022-00152-1","article-title":"The Relationship between the Gut Microbiome and the Risk of Respiratory Infections among Newborns","volume":"2","author":"Moroishi","year":"2022","journal-title":"Commun. Med."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1016\/j.jaci.2022.09.026","article-title":"Airway-Delivered Short-Chain Fatty Acid Acetate Boosts Antiviral Immunity during Rhinovirus Infection","volume":"151","author":"Antunes","year":"2023","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_72","doi-asserted-by":"crossref","unstructured":"Antunes, K.H., Stein, R.T., Franceschina, C., da Silva, E.F., de Freitas, D.N., Silveira, J., Mocellin, M., Leit\u00e3o, L., Fachi, J.L., and Pral, L.P. (2022). Short-Chain Fatty Acid Acetate Triggers Antiviral Response Mediated by RIG-I in Cells from Infants with Respiratory Syncytial Virus Bronchiolitis. EBioMedicine, 77.","DOI":"10.1016\/j.ebiom.2022.103891"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1021\/acsinfecdis.9b00359","article-title":"Haemophilus Influenzae Glucose Catabolism Leading to Production of the Immunometabolite Acetate Has a Key Contribution to the Host Airway\u2013Pathogen Interplay","volume":"6","author":"Euba","year":"2020","journal-title":"ACS Infect. Dis."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"773261","DOI":"10.3389\/fimmu.2022.773261","article-title":"Acetate Improves the Killing of Streptococcus Pneumoniae by Alveolar Macrophages via NLRP3 Inflammasome and Glycolysis-HIF-1\u03b1 Axis","volume":"13","author":"Machado","year":"2022","journal-title":"Front. Immunol."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"2331","DOI":"10.1021\/acsomega.8b03571","article-title":"Methionine Attenuates Lipopolysaccharide-Induced Inflammatory Responses via DNA Methylation in Macrophages","volume":"4","author":"Ji","year":"2019","journal-title":"ACS Omega"},{"key":"ref_76","doi-asserted-by":"crossref","unstructured":"Wu, X., Han, Z., Liu, B., Yu, D., Sun, J., Ge, L., Tang, W., and Liu, S. (2022). Gut Microbiota Contributes to the Methionine Metabolism in Host. Front. Microbiol., 13.","DOI":"10.3389\/fmicb.2022.1065668"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1136\/gutjnl-2015-309728","article-title":"The Gut Microbiota Plays a Protective Role in the Host Defence against Pneumococcal Pneumonia","volume":"65","author":"Schuijt","year":"2016","journal-title":"Gut"},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1038\/s41385-020-00361-8","article-title":"The Lung\u2013Gut Axis during Viral Respiratory Infections: The Impact of Gut Dysbiosis on Secondary Disease Outcomes","volume":"14","author":"Sencio","year":"2021","journal-title":"Mucosal Immunol."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"2640","DOI":"10.3389\/fimmu.2018.02640","article-title":"Respiratory Viral Infection-Induced Microbiome Alterations and Secondary Bacterial Pneumonia","volume":"9","author":"Hanada","year":"2018","journal-title":"Front. Immunol."},{"key":"ref_80","doi-asserted-by":"crossref","unstructured":"Heaney, L.M., Kang, S., Turner, M.A., Lindley, M.R., and Thomas, C.L.P. (2022). The Impact of a Graded Maximal Exercise Protocol on Exhaled Volatile Organic Compounds: A Pilot Study. Molecules, 27.","DOI":"10.3390\/molecules27020370"},{"key":"ref_81","doi-asserted-by":"crossref","unstructured":"Dragonieri, S., Quaranta, V.N., Portacci, A., Ahroud, M., Di Marco, M., Ranieri, T., and Carpagnano, G.E. (2023). Effect of Food Intake on Exhaled Volatile Organic Compounds Profile Analyzed by an Electronic Nose. Molecules, 28.","DOI":"10.1183\/13993003.congress-2023.PA5307"},{"key":"ref_82","doi-asserted-by":"crossref","unstructured":"Dragonieri, S., Quaranta, V.N., Buonamico, E., Battisti, C., Ranieri, T., Carratu, P., and Carpagnano, G.E. (2022). Short-Term Effect of Cigarette Smoke on Exhaled Volatile Organic Compounds Profile Analyzed by an Electronic Nose. Biosensors, 12.","DOI":"10.3390\/bios12070520"},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1080\/14737159.2021.1917998","article-title":"The Importance of Genomic Analysis in Cracking the Coronavirus Pandemic","volume":"21","author":"Zella","year":"2021","journal-title":"Expert Rev. Mol. Diagn."}],"container-title":["Antioxidants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-3921\/13\/2\/172\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T13:51:09Z","timestamp":1760104269000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-3921\/13\/2\/172"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1,29]]},"references-count":83,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2024,2]]}},"alternative-id":["antiox13020172"],"URL":"https:\/\/doi.org\/10.3390\/antiox13020172","relation":{"has-preprint":[{"id-type":"doi","id":"10.21203\/rs.3.rs-2858447\/v1","asserted-by":"object"}]},"ISSN":["2076-3921"],"issn-type":[{"value":"2076-3921","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,1,29]]}}}