{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T16:19:05Z","timestamp":1791994745172,"version":"4.4.0"},"reference-count":63,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2024,2,19]],"date-time":"2024-02-19T00:00:00Z","timestamp":1708300800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the National Key Research and Development Program, China","award":["2022YFD1300302"],"award-info":[{"award-number":["2022YFD1300302"]}]},{"name":"the National Key Research and Development Program, China","award":["KJCX20230218"],"award-info":[{"award-number":["KJCX20230218"]}]},{"name":"the National Key Research and Development Program, China","award":["BAIC05-2024"],"award-info":[{"award-number":["BAIC05-2024"]}]},{"name":"the Scientific &amp; Technological Innovation Ability Construction Project of the Beijing Academy of Agriculture and Forestry Sciences","award":["2022YFD1300302"],"award-info":[{"award-number":["2022YFD1300302"]}]},{"name":"the Scientific &amp; Technological Innovation Ability Construction Project of the Beijing Academy of Agriculture and Forestry Sciences","award":["KJCX20230218"],"award-info":[{"award-number":["KJCX20230218"]}]},{"name":"the Scientific &amp; Technological Innovation Ability Construction Project of the Beijing Academy of Agriculture and Forestry Sciences","award":["BAIC05-2024"],"award-info":[{"award-number":["BAIC05-2024"]}]},{"name":"the Beijing Innovation Consortium of Livestock Research System","award":["2022YFD1300302"],"award-info":[{"award-number":["2022YFD1300302"]}]},{"name":"the Beijing Innovation Consortium of Livestock Research System","award":["KJCX20230218"],"award-info":[{"award-number":["KJCX20230218"]}]},{"name":"the Beijing Innovation Consortium of Livestock Research System","award":["BAIC05-2024"],"award-info":[{"award-number":["BAIC05-2024"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>Postovulatory aging is known to impair the oocyte quality and embryo development due to oxidative stress in many different animal models, which reduces the success rate or pregnancy rate in human assisted reproductive technology (ART) and livestock timed artificial insemination (TAI), respectively. Salidroside (SAL), a phenylpropanoid glycoside, has been shown to exert antioxidant and antitumor effects. This study aimed to investigate whether SAL supplementation could delay the postovulatory oocyte aging process by alleviating oxidative stress. Here, we show that SAL supplementation decreases the malformation rate and recovers mitochondrial dysfunction including mitochondrial distribution, mitochondrial membrane potential (\u0394\u03a8) and ATP content in aged oocytes. In addition, SAL treatment alleviates postovulatory aging-caused oxidative stress such as higher reactive oxygen species (ROS) level, lower glutathione (GSH) content and a reduced expression of antioxidant-related genes. Moreover, the cytoplasmic calcium ([Ca2+]c) and mitochondrial calcium ([Ca2+]mt) of SAL-treated oocytes return to normal levels. Notably, SAL suppresses the aging-induced DNA damage, early apoptosis and improves spindle assembly in aged oocytes, ultimately elevating the embryo developmental rates and embryo quality. Finally, the RNA-seq and confirmatory experience showed that SAL promotes protective autophagy in aged oocytes by activating the MAPK pathway. Taken together, our research suggests that supplementing SAL is an effective and feasible method for preventing postovulatory aging and preserving the oocyte quality, which potentially contributes to improving the successful rate of ART or TAI.<\/jats:p>","DOI":"10.3390\/antiox13020248","type":"journal-article","created":{"date-parts":[[2024,2,19]],"date-time":"2024-02-19T10:40:15Z","timestamp":1708339215000},"page":"248","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["The Antioxidant Salidroside Ameliorates the Quality of Postovulatory Aged Oocyte and Embryo Development in Mice"],"prefix":"10.3390","volume":"13","author":[{"given":"Kexiong","family":"Liu","sequence":"first","affiliation":[{"name":"Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luyao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoling","family":"Xu","sequence":"additional","affiliation":[{"name":"Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linli","family":"Xiao","sequence":"additional","affiliation":[{"name":"Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junhui","family":"Wen","sequence":"additional","affiliation":[{"name":"Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanbing","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuxin","family":"Zhao","sequence":"additional","affiliation":[{"name":"Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongliang","family":"Qiao","sequence":"additional","affiliation":[{"name":"Development Center of Science and Technology, Ministry of Agriculture and Rural Affairs, Beijing 100176, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiahua","family":"Bai","sequence":"additional","affiliation":[{"name":"Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,2,19]]},"reference":[{"key":"ref_1","first-page":"367","article-title":"Study on the chemical constituents of Rhodiola crenulata","volume":"27","author":"Li","year":"2012","journal-title":"West China Med. 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