{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T22:10:47Z","timestamp":1787263847826,"version":"build-2736575974"},"reference-count":51,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2024,3,7]],"date-time":"2024-03-07T00:00:00Z","timestamp":1709769600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea (NRF)","doi-asserted-by":"publisher","award":["2019R1I1A2A01045132"],"award-info":[{"award-number":["2019R1I1A2A01045132"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>The total melanin synthesis in the skin depends on various melanogenic factors, including the number of viable melanocytes, the level of melanogenic enzymes per cell, and the reaction rate of the enzymes. The purpose of this study is to examine the effects of L-cysteine (L-Cys), L-ascorbic acid (L-AA), and their derivatives on the tyrosinase (TYR) activity and autoxidation of L-3,4-dihydroxyphenylalanine (L-DOPA) in vitro and the viability and melanin synthesis of B16\/F10 cells under different conditions. L-Cysteinamide (C-NH2), glutathione (GSH), L-Cys, L-AA, and N-acetyl L-cysteine (NAC) inhibited the catalytic activity of TYR in vitro. L-AA, C-NH2, L-ascorbic acid 2-O-glucoside (AAG), and 3-O-ethyl L-ascorbic acid (EAA) inhibited the autoxidation of L-DOPA in vitro. L-DOPA exhibited cytotoxicity at 0.1 mM and higher concentrations, whereas L-tyrosine (L-Tyr) did not affect cell viability up to 3 mM. L-AA, magnesium L-ascorbyl 2-phosphate (MAP), and L-Cys attenuated the cell death induced by L-DOPA. C-NH2 decreased the intracellular melanin level at the basal state, whereas L-AA, MAP, and AAG conversely increased it. C-NH2 reduced the number of darkly pigmented cells via in situ L-DOPA staining, whereas L-AA, MAP, GSH, and AAG increased it. C-NH2 decreased the intracellular melanin level at the alpha-melanocyte-stimulating hormone (\u03b1-MSH)-stimulated state, while NAC and GSH increased it. L-AA and C-NH2 decreased the intracellular melanin level at the L-Tyr-stimulated state, but NAC and GSH increased it. L-Ascorbyl tetraisopalmitate (ATI) showed no or minor effects in most experiments. This study suggests that L-AA can either promote or inhibit the different melanogenic factors, and C-NH2 can inhibit the multiple melanogenic factors consistently. This study highlights the multifaceted properties of L-Cys, L-AA, and their derivatives that can direct their therapeutic applications in hyperpigmentation, hypopigmentation, or both disorders.<\/jats:p>","DOI":"10.3390\/antiox13030330","type":"journal-article","created":{"date-parts":[[2024,3,7]],"date-time":"2024-03-07T08:59:37Z","timestamp":1709801977000},"page":"330","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Multifaceted Effects of L-Cysteine, L-Ascorbic Acid, and Their Derivatives on the Viability and Melanin Synthesis of B16\/F10 Cells under Different Conditions"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-9810-8107","authenticated-orcid":false,"given":"Joon Yong","family":"Choi","sequence":"first","affiliation":[{"name":"Department of Biomedical Science, The Graduate School, Kyungpook National University, 680 Gukchaebosang-ro, Jung-gu, Daegu 41944, Republic of Korea"},{"name":"BK21 Plus KNU Biomedical Convergence Program, Kyungpook National University, 680 Gukchaebosang-ro, Jung-gu, Daegu 41944, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0720-6338","authenticated-orcid":false,"given":"Jae Won","family":"Ha","sequence":"additional","affiliation":[{"name":"Department of Biomedical Science, The Graduate School, Kyungpook National University, 680 Gukchaebosang-ro, Jung-gu, Daegu 41944, Republic of Korea"},{"name":"BK21 Plus KNU Biomedical Convergence Program, Kyungpook National University, 680 Gukchaebosang-ro, Jung-gu, Daegu 41944, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9895-2066","authenticated-orcid":false,"given":"Yong Chool","family":"Boo","sequence":"additional","affiliation":[{"name":"Department of Biomedical Science, The Graduate School, Kyungpook National University, 680 Gukchaebosang-ro, Jung-gu, Daegu 41944, Republic of Korea"},{"name":"BK21 Plus KNU Biomedical Convergence Program, Kyungpook National University, 680 Gukchaebosang-ro, Jung-gu, Daegu 41944, Republic of Korea"},{"name":"Department of Molecular Medicine, School of Medicine, Kyungpook National University, 680 Gukchaebosang-ro, Jung-gu, Daegu 41944, Republic of Korea"},{"name":"Cell and Matrix Research Institute, Kyungpook National University, 680 Gukchaebosang-ro, Jung-gu, Daegu 41944, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,3,7]]},"reference":[{"key":"ref_1","first-page":"4183","article-title":"Synthesis and physiological implications of melanic pigments","volume":"17","author":"Maranduca","year":"2019","journal-title":"Oncol. Lett."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Moreiras, H., Seabra, M.C., and Barral, D.C. (2021). Melanin Transfer in the Epidermis: The Pursuit of Skin Pigmentation Control Mechanisms. Int. J. Mol. Sci., 22.","DOI":"10.20944\/preprints202104.0441.v1"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Lu, Y.Y., Tonissen, K.F., and Di Trapani, G. (2021). Modulating skin colour: Role of the thioredoxin and glutathione systems in regulating melanogenesis. Biosci. Rep., 41.","DOI":"10.1042\/BSR20210427"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"279","DOI":"10.5021\/ad.2016.28.3.279","article-title":"Skin Pigmentation and Pigmentary Disorders: Focus on Epidermal\/Dermal Cross-Talk","volume":"28","author":"Bastonini","year":"2016","journal-title":"Ann. Dermatol."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Gelmi, M.C., Houtzagers, L.E., Strub, T., Krossa, I., and Jager, M.J. (2022). MITF in Normal Melanocytes, Cutaneous and Uveal Melanoma: A Delicate Balance. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23116001"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"F1000 Faculty Rev-608","DOI":"10.12688\/f1000research.24625.1","article-title":"Recent advances in understanding the molecular basis of melanogenesis in melanocytes","volume":"9","author":"Ohbayashi","year":"2020","journal-title":"F1000Research"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1080\/14756366.2016.1256882","article-title":"Skin whitening agents: Medicinal chemistry perspective of tyrosinase inhibitors","volume":"32","author":"Pillaiyar","year":"2017","journal-title":"J. Enzym. Inhib. Med. Chem."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1159\/000518826","article-title":"Influence of Ethnicities and Skin Color Variations in Different Populations: A Review","volume":"35","author":"Naik","year":"2022","journal-title":"Ski. Pharmacol. Physiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1016\/0304-4165(88)90023-2","article-title":"The role of sulfhydryl compounds in mammalian melanogenesis: The effect of cysteine and glutathione upon tyrosinase and the intermediates of the pathway","volume":"967","author":"Jara","year":"1988","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"155","DOI":"10.2340\/0001555564155157","article-title":"Inactivation of human tyrosinase by cysteine. Protection by dopa and tyrosine","volume":"64","author":"Jergil","year":"1984","journal-title":"Acta Derm. Venereol."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Boo, Y.C. (2022). Metabolic Basis and Clinical Evidence for Skin Lightening Effects of Thiol Compounds. Antioxidants, 11.","DOI":"10.3390\/antiox11030503"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Lee, H.K., Ha, J.W., Hwang, Y.J., and Boo, Y.C. (2021). Identification of L-Cysteinamide as a Potent Inhibitor of Tyrosinase-Mediated Dopachrome Formation and Eumelanin Synthesis. Antioxidants, 10.","DOI":"10.3390\/antiox10081202"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"209","DOI":"10.2340\/0001555563209214","article-title":"The effect of catalase on the inactivation of tyrosinase by ascorbic acid and by cysteine or glutathione","volume":"63","author":"Lindbladh","year":"1983","journal-title":"Acta Derm. Venereol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1042\/bj2950309","article-title":"Effect of L-ascorbic acid on the monophenolase activity of tyrosinase","volume":"295","author":"Ros","year":"1993","journal-title":"Biochem. J."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"669","DOI":"10.1007\/s00403-011-1158-4","article-title":"Ascorbic acid increases the activity and synthesis of tyrosinase in B16F10 cells through activation of p38 mitogen-activated protein kinase","volume":"303","author":"Lee","year":"2011","journal-title":"Arch. Dermatol. Res."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"374","DOI":"10.4103\/jomfp.JOMFP_207_20","article-title":"The effect of Vitamin C on melanin pigmentation\u2014A systematic review","volume":"24","author":"Sanadi","year":"2020","journal-title":"J. Oral Maxillofac. Pathol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/S0190-9622(96)90830-0","article-title":"Inhibitory effect of magnesium L-ascorbyl-2-phosphate (VC-PMG) on melanogenesis in vitro and in vivo","volume":"34","author":"Kameyama","year":"1996","journal-title":"J. Am. Acad. Dermatol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1902\/jop.2009.080409","article-title":"Effects of ascorbic acid on gingival melanin pigmentation in vitro and in vivo","volume":"80","author":"Shimada","year":"2009","journal-title":"J. Periodontol."},{"key":"ref_19","first-page":"233","article-title":"Antioxidant Ability and Stability Studies of 3-O-Ethyl Ascorbic Acid, a Cosmetic Tyrosinase Inhibitor","volume":"69","author":"Liao","year":"2018","journal-title":"J. Cosmet. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"3503","DOI":"10.1111\/jocd.14604","article-title":"Evaluation of the anti-wrinkle effect of a lipophilic pro-vitamin C derivative, tetra-isopalmitoyl ascorbic acid","volume":"21","author":"Yokota","year":"2021","journal-title":"J. Cosmet. Dermatol."},{"key":"ref_21","first-page":"1821780","article-title":"Research Progress on Targeted Antioxidant Therapy and Vitiligo","volume":"2022","author":"Zhang","year":"2022","journal-title":"Oxidative Med. Cell. Longev."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"7881717","DOI":"10.1155\/2022\/7881717","article-title":"Implications of Oxidative Stress in the Pathogenesis and Treatment of Hyperpigmentation Disorders","volume":"2022","author":"Xing","year":"2022","journal-title":"Oxidative Med. Cell. Longev."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"560","DOI":"10.1111\/exd.14260","article-title":"Skin pigmentation and its control: From ultraviolet radiation to stem cells","volume":"30","author":"Fisher","year":"2021","journal-title":"Exp. Dermatol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"105063","DOI":"10.1016\/j.envint.2019.105063","article-title":"Potential role of PM2.5 in melanogenesis","volume":"132","author":"Peng","year":"2019","journal-title":"Environ. Int."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1038\/s41420-022-00863-3","article-title":"Oxeiptosis: A novel pathway of melanocytes death in response to oxidative stress in vitiligo","volume":"8","author":"Kang","year":"2022","journal-title":"Cell Death Discov."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Diotallevi, F., Gioacchini, H., De Simoni, E., Marani, A., Candelora, M., Paolinelli, M., Molinelli, E., Offidani, A., and Simonetti, O. (2023). Vitiligo, from Pathogenesis to Therapeutic Advances: State of the Art. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24054910"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Boo, Y.C. (2020). Up- or Downregulation of Melanin Synthesis Using Amino Acids, Peptides, and Their Analogs. Biomedicines, 8.","DOI":"10.3390\/biomedicines8090322"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/j.jdermsci.2007.03.009","article-title":"Glutathione prevented dopamine-induced apoptosis of melanocytes and its signaling","volume":"47","author":"Park","year":"2007","journal-title":"J. Dermatol. Sci."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Garcia-Jimenez, A., Teruel-Puche, J.A., Berna, J., Rodriguez-Lopez, J.N., Tudela, J., and Garcia-Canovas, F. (2017). Action of tyrosinase on alpha and beta-arbutin: A kinetic study. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0177330"},{"key":"ref_30","first-page":"515","article-title":"Use of non-melanocytic HEK293 cells stably expressing human tyrosinase for the screening of anti-melanogenic agents","volume":"62","author":"Kim","year":"2011","journal-title":"J. Cosmet. Sci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1016\/j.sajb.2018.09.025","article-title":"In vitro antioxidant activity, inhibitory effect of tyrosinase and DOPA auto-oxidation by Wrightia religiosa extracts","volume":"120","author":"Klomsakul","year":"2019","journal-title":"S. Afr. J. Bot."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/j.acthis.2018.02.005","article-title":"Tetrazolium salts and formazan products in Cell Biology: Viability assessment, fluorescence imaging, and labeling perspectives","volume":"120","author":"Stockert","year":"2018","journal-title":"Acta Histochem."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1111\/bjd.17634","article-title":"Identification of small peptides and glycinamide that inhibit melanin synthesis using a positional scanning synthetic peptide combinatorial library","volume":"181","author":"Kim","year":"2019","journal-title":"Br. J. Dermatol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"2216","DOI":"10.1038\/sj.jid.5700840","article-title":"Post-transcriptional regulation of melanin biosynthetic enzymes by cAMP and resveratrol in human melanocytes","volume":"127","author":"Newton","year":"2007","journal-title":"J. Investig. Dermatol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/j.neuropharm.2012.11.010","article-title":"L-dopa-induced dopamine synthesis and oxidative stress in serotonergic cells","volume":"67","author":"Stansley","year":"2013","journal-title":"Neuropharmacology"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1038\/s41420-021-00547-4","article-title":"Impairment of neuronal mitochondrial function by l-DOPA in the absence of oxygen-dependent auto-oxidation and oxidative cell damage","volume":"7","author":"Delcambre","year":"2021","journal-title":"Cell Death Discov."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Hida, T., Kamiya, T., Kawakami, A., Ogino, J., Sohma, H., Uhara, H., and Jimbow, K. (2020). Elucidation of Melanogenesis Cascade for Identifying Pathophysiology and Therapeutic Approach of Pigmentary Disorders and Melanoma. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21176129"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"796","DOI":"10.1111\/1523-1747.ep12340980","article-title":"Melanogenesis in cultured melanocytes can be substantially influenced by L-tyrosine and L-cysteine","volume":"109","author":"Smit","year":"1997","journal-title":"J. Investig. Dermatol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"359","DOI":"10.3389\/fonc.2014.00359","article-title":"Ascorbate as a co-factor for Fe- and 2-oxoglutarate dependent dioxygenases: Physiological activity in tumor growth and progression","volume":"4","author":"Kuiper","year":"2014","journal-title":"Front. Oncol."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Boo, Y.C. (2022). Ascorbic Acid (Vitamin C) as a Cosmeceutical to Increase Dermal Collagen for Skin Antiaging Purposes: Emerging Combination Therapies. Antioxidants, 11.","DOI":"10.3390\/antiox11091663"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1076","DOI":"10.1248\/bpb.18.1076","article-title":"Inhibitory Effect of Sodium 5,6-Benzylidene Ascorbate (Sba) on the Elevation of Melanin Biosynthesis Induced by Ultraviolet-a (Uv-a) Light in Cultured B-16 Melanoma-Cells","volume":"18","author":"Kojima","year":"1995","journal-title":"Biol. Pharm. Bull."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1248\/cpb.56.292","article-title":"Inhibitory effects of a novel ascorbic derivative, disodium isostearyl 2-O-L-ascorbyl phosphate on melanogenesis","volume":"56","author":"Matsuda","year":"2008","journal-title":"Chem. Pharm. Bull."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1209","DOI":"10.1111\/jocd.12451","article-title":"3-O-Glyceryl-2-O-hexyl ascorbate suppresses melanogenesis by interfering with intracellular melanosome transport and suppressing tyrosinase protein synthesis","volume":"17","author":"Taira","year":"2018","journal-title":"J. Cosmet. Dermatol."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Taira, N., Katsuyama, Y., Yoshioka, M., Muraoka, O., and Morikawa, T. (2018). Structural Requirements of Alkylglyceryl-l-Ascorbic Acid Derivatives for Melanogenesis Inhibitory Activity. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19041144"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1007\/s00403-015-1583-x","article-title":"Ascorbyl coumarates as multifunctional cosmeceutical agents that inhibit melanogenesis and enhance collagen synthesis","volume":"307","author":"Kwak","year":"2015","journal-title":"Arch. Dermatol. Res."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"652","DOI":"10.4103\/ijdvl.IJDVL_33_18","article-title":"N-acetylcysteine in dermatology","volume":"84","author":"Adil","year":"2018","journal-title":"Indian J. Dermatol. Venereol. Leprol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"2291","DOI":"10.1083\/jcb.201804161","article-title":"Glutathione metabolism in cancer progression and treatment resistance","volume":"217","author":"Bansal","year":"2018","journal-title":"J. Cell Biol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1016\/j.redox.2015.04.004","article-title":"Thiol redox homeostasis in neurodegenerative disease","volume":"5","author":"McBean","year":"2015","journal-title":"Redox Biol."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Gaucher, C., Boudier, A., Bonetti, J., Clarot, I., Leroy, P., and Parent, M. (2018). Glutathione: Antioxidant Properties Dedicated to Nanotechnologies. Antioxidants, 7.","DOI":"10.3390\/antiox7050062"},{"key":"ref_50","first-page":"119","article-title":"Mechanisms of Laser-Tissue Interaction: I. Optical Properties of Tissue","volume":"2","author":"Mohajerani","year":"2011","journal-title":"J. Lasers Med. Sci."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/j.jchromb.2019.03.010","article-title":"Determination of eumelanin and pheomelanin in melanomas using solid-phase extraction and high performance liquid chromatography-diode array detection","volume":"1113","author":"Rioux","year":"2019","journal-title":"J. Chromatogr. 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