{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T01:13:00Z","timestamp":1793149980887,"version":"4.5.0"},"reference-count":72,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2024,5,8]],"date-time":"2024-05-08T00:00:00Z","timestamp":1715126400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Key Research and Development Program of China","award":["2021YFD1800402"],"award-info":[{"award-number":["2021YFD1800402"]}]},{"name":"National Key Research and Development Program of China","award":["32202807"],"award-info":[{"award-number":["32202807"]}]},{"name":"National Key Research and Development Program of China","award":["2022M723466"],"award-info":[{"award-number":["2022M723466"]}]},{"name":"National Key Research and Development Program of China","award":["2021447"],"award-info":[{"award-number":["2021447"]}]},{"name":"National Natural Science Foundation of China","award":["2021YFD1800402"],"award-info":[{"award-number":["2021YFD1800402"]}]},{"name":"National Natural Science Foundation of China","award":["32202807"],"award-info":[{"award-number":["32202807"]}]},{"name":"National Natural Science Foundation of China","award":["2022M723466"],"award-info":[{"award-number":["2022M723466"]}]},{"name":"National Natural Science Foundation of China","award":["2021447"],"award-info":[{"award-number":["2021447"]}]},{"name":"China Postdoctoral Science Foundation","award":["2021YFD1800402"],"award-info":[{"award-number":["2021YFD1800402"]}]},{"name":"China Postdoctoral Science Foundation","award":["32202807"],"award-info":[{"award-number":["32202807"]}]},{"name":"China Postdoctoral Science Foundation","award":["2022M723466"],"award-info":[{"award-number":["2022M723466"]}]},{"name":"China Postdoctoral Science Foundation","award":["2021447"],"award-info":[{"award-number":["2021447"]}]},{"name":"Shanghai Postdoctoral Excellence Program","award":["2021YFD1800402"],"award-info":[{"award-number":["2021YFD1800402"]}]},{"name":"Shanghai Postdoctoral Excellence Program","award":["32202807"],"award-info":[{"award-number":["32202807"]}]},{"name":"Shanghai Postdoctoral Excellence Program","award":["2022M723466"],"award-info":[{"award-number":["2022M723466"]}]},{"name":"Shanghai Postdoctoral Excellence Program","award":["2021447"],"award-info":[{"award-number":["2021447"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>Brucella virulence relies on its successful intracellular life cycle. Modulating host cell death is a strategy for Brucella to survive and replicate intracellularly. Ferroptosis is a novel regulated cell death characterized by iron-triggered excessive lipid peroxidation, which has been proven to be associated with pathogenic bacteria infection. Thus, we attempted to explore if smooth-type Brucella infection triggers host cell ferroptosis and what role it plays in Brucella infection. We assessed the effects of Brucella infection on the lactate dehydrogenase release and lipid peroxidation levels of RAW264.7 macrophages; subsequently, we determined the effect of Brucella infection on the expressions of ferroptosis defense pathways. Furthermore, we determined the role of host cell ferroptosis in the intracellular replication and egress of Brucella. The results demonstrated that Brucella M5 could induce ferroptosis of macrophages by inhibiting the GPX4-GSH axis at the late stage of infection but mitigated ferroptosis by up-regulating the GCH1-BH4 axis at the early infection stage. Moreover, elevating host cell ferroptosis decreased Brucella intracellular survival and suppressing host cell ferroptosis increased Brucella intracellular replication and egress. Collectively, Brucella may manipulate host cell ferroptosis to facilitate its intracellular replication and egress, extending our knowledge about the underlying mechanism of how Brucella completes its intracellular life cycle.<\/jats:p>","DOI":"10.3390\/antiox13050577","type":"journal-article","created":{"date-parts":[[2024,5,8]],"date-time":"2024-05-08T12:27:11Z","timestamp":1715171231000},"page":"577","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Brucella Manipulates Host Cell Ferroptosis to Facilitate Its Intracellular Replication and Egress in RAW264.7 Macrophages"],"prefix":"10.3390","volume":"13","author":[{"given":"Guangdong","family":"Zhang","sequence":"first","affiliation":[{"name":"Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Shanghai 200241, China"},{"name":"Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Harbin 150069, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hai","family":"Hu","sequence":"additional","affiliation":[{"name":"Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Shanghai 200241, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Yin","sequence":"additional","affiliation":[{"name":"Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Shanghai 200241, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingxing","family":"Tian","sequence":"additional","affiliation":[{"name":"Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Shanghai 200241, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhigao","family":"Bu","sequence":"additional","affiliation":[{"name":"Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Harbin 150069, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chan","family":"Ding","sequence":"additional","affiliation":[{"name":"Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Shanghai 200241, China"},{"name":"Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shengqing","family":"Yu","sequence":"additional","affiliation":[{"name":"Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Shanghai 200241, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,5,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1046\/j.1462-5822.2001.00102.x","article-title":"Essential role of the VirB machinery in the maturation of the Brucella abortus-containing vacuole","volume":"3","author":"Comerci","year":"2001","journal-title":"Cell Microbiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1016\/S1473-3099(07)70286-4","article-title":"Human brucellosis","volume":"7","author":"Franco","year":"2007","journal-title":"Lancet Infect. 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