{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T04:33:39Z","timestamp":1790224419381,"version":"4.1.0"},"reference-count":88,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2024,5,25]],"date-time":"2024-05-25T00:00:00Z","timestamp":1716595200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Medical University of Lublin (Polish Ministry of Science)","award":["DS 28"],"award-info":[{"award-number":["DS 28"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>The present study was performed to determine the chemical constituents, cytotoxicity, antioxidant and enzyme inhibition activities of the aerial parts of Glaucium acutidentatum Hausskn. and Bornm. (family Papaveraceae). Methanolic and aqueous extracts were prepared by maceration, homogenizer-assisted extraction (HAE) and infusion. Results showed that the highest total phenolic and flavonoids contents were obtained from the methanol extracts obtained by HAE (53.22 \u00b1 0.10 mg GAE\/g) and maceration (30.28 \u00b1 0.51 mg RE\/g), respectively. The aporphine, beznyltetrahydroisoquinoline, and protopine types of Glaucium alkaloids have been tentatively identified. Among them, glaucine was identified in all extracts. Flavonoids, phenolic acids, coumarins, organic acids and fatty acids were also detected. Methanolic extract obtained using the HAE method displayed the highest anti-DPPH (41.42 \u00b1 0.62 mg TE\/g), total antioxidant (1.20 \u00b1 0.17 mmol TE\/g), Cu2+ (113.55 \u00b1 6.44 mg TE\/g), and Fe3+ (74.52 \u00b1 4.74 mg TE\/g) reducing properties. The aqueous extracts obtained by infusion and HAE methods exerted the best anti-ABTS (103.59 \u00b1 1.49 mg TE\/g) and chelating (19.81 \u00b1 0.05 mg EDTAE\/g) activities, respectively. Methanolic extract from HAE recorded the highest acetylcholinesterase (2.55 \u00b1 0.10 mg GALAE\/g) and \u03b1-amylase (0.51 \u00b1 0.02 mmol ACAE\/g) inhibition activities, while that obtained by maceration showed the best butyrylcholinesterase (3.76 \u00b1 0.31 mg GALAE\/g) inhibition activity. Both extracts revealed the best tyrosinase inhibitory activity (25.15 \u00b1 1.00 and 26.79 \u00b1 2.36 mg KAE\/g, p \u2265 0.05). G. acutidentatum maceration-derived aqueous extract showed selective anticancer activity against cells originating from human hypopharyngeal carcinoma. In conclusion, these findings indicated that G. acutidentatum is a promising source of alkaloids and phenolic compounds for variable pharmaceutical formulations.<\/jats:p>","DOI":"10.3390\/antiox13060643","type":"journal-article","created":{"date-parts":[[2024,5,27]],"date-time":"2024-05-27T10:14:07Z","timestamp":1716804847000},"page":"643","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Exploring Chemical Composition, Antioxidant, Enzyme Inhibitory and Cytotoxic Properties of Glaucium acutidentatum Hausskn. &amp; Bornm. from Turkey Flora: A Novel Source of Bioactive Agents to Design Functional Applications"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9600-3526","authenticated-orcid":false,"given":"Sakina","family":"Yagi","sequence":"first","affiliation":[{"name":"Department of Botany, Faculty of Science, University of Khartoum, Khartoum 11115, Sudan"},{"name":"LAE, INRAE, Universit\u00e9 de Lorraine, F-54000 Nancy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6548-7823","authenticated-orcid":false,"given":"Gokhan","family":"Zengin","sequence":"additional","affiliation":[{"name":"Physiology and Biochemistry Laboratory, Department of Biology, Science Faculty, Selcuk University, Konya 42130, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0853-108X","authenticated-orcid":false,"given":"Abdullahi Ibrahim","family":"Uba","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Genetics, Istanbul AREL University, Istanbul 34537, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4113-9079","authenticated-orcid":false,"given":"Magdalena","family":"Maciejewska-Turska","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy with Medicinal Plant Garden, Medical University of Lublin, 20-093 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4773-0329","authenticated-orcid":false,"given":"Elwira","family":"Sieniawska","sequence":"additional","affiliation":[{"name":"Department of Natural Products Chemistry, Medical University of Lublin, 20-093 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1626-680X","authenticated-orcid":false,"given":"\u0141ukasz","family":"\u015awi\u0105tek","sequence":"additional","affiliation":[{"name":"Department of Virology with Viral Diagnostics Laboratory, Medical University of Lublin, 20-093 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8857-8688","authenticated-orcid":false,"given":"Barbara","family":"Rajtar","sequence":"additional","affiliation":[{"name":"Department of Virology with Viral Diagnostics Laboratory, Medical University of Lublin, 20-093 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muammer","family":"Bah\u015fi","sequence":"additional","affiliation":[{"name":"Department of Primary Education, Faculty of Education, F\u0131rat University, Elaz\u0131g 23119, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8225-1505","authenticated-orcid":false,"given":"Osman","family":"Guler","sequence":"additional","affiliation":[{"name":"Pertek Sakine Gen\u00e7 Vocational School, Munzur University, Tunceli 62500, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8264-6953","authenticated-orcid":false,"given":"Stefano","family":"Dall\u2019Acqua","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical and Pharmacological Sciences, University of Padova, 35131 Padua, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3222-184X","authenticated-orcid":false,"given":"Ma\u0142gorzata","family":"Polz-Dacewicz","sequence":"additional","affiliation":[{"name":"Department of Virology with Viral Diagnostics Laboratory, Medical University of Lublin, 20-093 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,5,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"57","DOI":"10.2478\/botcro-2019-0003","article-title":"A taxonomic revision of the genus Glaucium (Papaveraceae) in Iran","volume":"78","author":"Tavakkoli","year":"2019","journal-title":"Acta Bot. 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