{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T16:31:57Z","timestamp":1787589117629,"version":"build-2736575974"},"reference-count":36,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2024,6,25]],"date-time":"2024-06-25T00:00:00Z","timestamp":1719273600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Research Foundation of Korea","award":["NRF-2019R1A6A1A03031807"],"award-info":[{"award-number":["NRF-2019R1A6A1A03031807"]}]},{"name":"National Research Foundation of Korea","award":["NRF2021R1A2C1093814"],"award-info":[{"award-number":["NRF2021R1A2C1093814"]}]},{"name":"National Research Foundation of Korea","award":["IITP-2023-RS-2023-00258971"],"award-info":[{"award-number":["IITP-2023-RS-2023-00258971"]}]},{"name":"MSIT (Ministry of Science and ICT)","award":["NRF-2019R1A6A1A03031807"],"award-info":[{"award-number":["NRF-2019R1A6A1A03031807"]}]},{"name":"MSIT (Ministry of Science and ICT)","award":["NRF2021R1A2C1093814"],"award-info":[{"award-number":["NRF2021R1A2C1093814"]}]},{"name":"MSIT (Ministry of Science and ICT)","award":["IITP-2023-RS-2023-00258971"],"award-info":[{"award-number":["IITP-2023-RS-2023-00258971"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>Interleukin (IL)-33, a member of the IL-1 cytokine family, plays a vital role in immune system regulation and inflammation, with oxidative stress being implicated in its expression. During the search for compounds from natural sources with potential as therapeutic agents for allergic diseases via IL-33 signal modulation, we discovered significant IL-33 inhibitory activity in the methanol extract of Canavalia gladiata (sword bean) pods. Through chromatographic separation and liquid chromatography\u2013mass spectrometry, we isolated 11 compounds (1\u201311) from the methanol extract. Furthermore, we assessed the inhibitory effects of these substances on IL-33\/ST2 signaling in processes related to inflammatory and autoimmune diseases using an enzyme-linked immunosorbent assay. Among them, compounds 7, 10, and 11 exhibited substantial IL-33 inhibitory efficacy, with values reaching 78%, 86%, and 79% at 100 \u00b5M, respectively. Remarkably, compounds 7, 10, and 11 demonstrated significant and dose-dependent inhibition of IL-33 signaling at concentrations of 10, 50, and 100 \u00b5M. Computational molecular docking and dynamic simulations further elucidated the underlying mechanisms. These findings have promising pharmacological implications for allergy prevention and treatment associated with flavonoid glycosides derived from C. gladiata.<\/jats:p>","DOI":"10.3390\/antiox13070767","type":"journal-article","created":{"date-parts":[[2024,6,27]],"date-time":"2024-06-27T05:11:39Z","timestamp":1719465099000},"page":"767","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Identification of Interleukin (IL)-33 Inhibitory Constituents from Canavalia gladiata Pods"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0564-2606","authenticated-orcid":false,"given":"Le Ba","family":"Vinh","sequence":"first","affiliation":[{"name":"College of Pharmacy, Korea University, Sejong 30019, Republic of Korea"},{"name":"Institute of Marine Biochemistry, Vietnam Academy of Science and Technology, Hanoi 11355, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seung Hyuck","family":"Shin","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Korea University, Sejong 30019, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoo Kyong","family":"Han","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Korea University, Sejong 30019, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-8063-8618","authenticated-orcid":false,"given":"Young Jun","family":"Kim","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Korea University, Sejong 30019, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2190-6648","authenticated-orcid":false,"given":"Nguyen Cao","family":"Cuong","sequence":"additional","affiliation":[{"name":"Faculty of Medicine and Pharmacy, Yersin University, Da Lat 66100, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4860-034X","authenticated-orcid":false,"given":"Soohwan","family":"Oh","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Korea University, Sejong 30019, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6191-4485","authenticated-orcid":false,"given":"Ki Yong","family":"Lee","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Korea University, Sejong 30019, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,6,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Kubo, T., Morita, H., Sugita, K., and Akdis, C.A. 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