{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T21:14:48Z","timestamp":1784322888887,"version":"3.55.0"},"reference-count":81,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2024,7,24]],"date-time":"2024-07-24T00:00:00Z","timestamp":1721779200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Construction of Standardized Production System for Improving quality and efficiency of Tibetan sheep industry","award":["2022-NK-169-1"],"award-info":[{"award-number":["2022-NK-169-1"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>Previous research studies confirmed that both resveratrol (RES) and \u03b2-hydroxy-\u03b2-methyl butyric acid (HMB) improved growth performance by altering intestinal microbiota. However, the mechanism underlying of RES and HMB on intestinal function remains unclear in ruminant. In this study, supplements of RES and HMB alone or in combination were evaluated as promoters of antioxidant capacity, immune response and barrier function, and modulators of the microbiota and metabolite profiles in the jejunum of Tibetan sheep. A total of 120 two-month-old Tibetan rams were randomly divided into four treatments (n = 30 per treatment), which were supplemented with a basal diet with 1.5 g RES\/d (RES group), 1.25 g HMB\/d (HMB group), 1.5 g RES\/d plus 1.25 g HMB\/d (RES-HMB group), and without additions (Control group). The results showed that RES and HMB improved the antioxidant capacity (CAT, GSH-Px, SOD, and T-AOC), immunity (IgA, IgG, and IgM), and digestive enzyme activity (\u03b1-amylase, lipase, and chymotrypsin) of the experimental lambs (p &lt; 0.05). Additionally, jejunal morphology including villus width, villus height, and muscle layer thickness exhibited a significant difference when rams were fed diets supplemented with RES and HMB (p &lt; 0.05). Furthermore, the determination of fermentation parameters showed that the butyrate concentration in the RES-HMB group was greater than those in the C and RES groups (p &lt; 0.05). When compared to the C group, barrier-related gene expression (MUC-2, ZO-1, and IL-10) was significantly increased in the RES-HMB group (p &lt; 0.05). Dietary RES and (or) HMB supplementation significantly increased the abundance of Methanobrevibacter, Actinobacteriota and Bacillus (p &lt; 0.05). The abundance of differential bacteria was positively associated with butyrate concentration (p &lt; 0.05). Metabolome analysis revealed that alpha ketoglutarate, succinic semialdehyde, and diacetyl as well as butanoate metabolism pathways connected to the improvements in butyrate concentration by RES and (or) HMB supplementation. Collectively, our results suggested that RES and (or) HMB supplementation improved butyrate concentration via regulating the microbial community (Methanobrevibacter, Actinobacteriota and Bacillus) and metabolism (alpha ketoglutarate, succinic semialdehyde, and diacetyl), thus contributing to jejunal morphology, antioxidant capacity, immune response, digestive enzyme activity, and barrier function.<\/jats:p>","DOI":"10.3390\/antiox13080892","type":"journal-article","created":{"date-parts":[[2024,7,24]],"date-time":"2024-07-24T15:47:32Z","timestamp":1721836052000},"page":"892","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Multi-Omics Revealed Resveratrol and \u03b2-Hydroxy-\u03b2-methyl Butyric Acid Alone or in Combination Improved the Jejunal Function in Tibetan Sheep"],"prefix":"10.3390","volume":"13","author":[{"given":"Qiurong","family":"Ji","sequence":"first","affiliation":[{"name":"College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fengshuo","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quyangangmao","family":"Su","sequence":"additional","affiliation":[{"name":"College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingli","family":"He","sequence":"additional","affiliation":[{"name":"College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shengzhen","family":"Hou","sequence":"additional","affiliation":[{"name":"College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6392-3513","authenticated-orcid":false,"given":"Linsheng","family":"Gui","sequence":"additional","affiliation":[{"name":"College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,7,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1308","DOI":"10.1038\/labinvest.3700330","article-title":"Enteropathogenic E. coli disrupts tight junction barrier function and structure in vivo","volume":"85","author":"Shifflett","year":"2005","journal-title":"Lab. Investig."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Ma, Y., Yang, X., Hua, G., Deng, X., Xia, T., Li, X., Feng, D., and Deng, X. (2022). Contribution of gut microbiomes and their metabolomes to the performance of Dorper and Tan sheep. Front. Microbiol., 13.","DOI":"10.3389\/fmicb.2022.1047744"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"853","DOI":"10.1136\/gutjnl-2020-320951","article-title":"Multiomics analysis reveals the presence of a microbiome in the gut of fetal lambs","volume":"70","author":"Bi","year":"2021","journal-title":"Gut"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Su, S., Wang, L., Fu, S., Zhao, J., He, X., Chen, Q., Belobrajdic, D.P., Yu, C., Liu, H., and Wu, H. (2022). Effects of oat (Avena sativa L.) hay diet supplementation on the intestinal microbiome and metabolome of Small-tail Han sheep. Front. Microbiol., 13.","DOI":"10.3389\/fmicb.2022.1032622"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1007\/s11250-023-03752-8","article-title":"Improving the productive performance of growing lambs using prebiotic and probiotic as growth promoters","volume":"55","author":"Shoukry","year":"2023","journal-title":"Trop. Anim. Health Prod."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Shaito, A., Posadino, A.M., Younes, N., Hasan, H., Halabi, S., Alhababi, D., Al-Mohannadi, A., Abdel-Rahman, W.M., Eid, A.H., and Nasrallah, G.K. (2020). Potential Adverse Effects of Resveratrol: A Literature Review. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21062084"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1111\/jre.12622","article-title":"Resveratrol attenuates oxidative stress during experimental periodontitis in rats exposed to cigarette smoke inhalation","volume":"54","author":"Absy","year":"2019","journal-title":"J. Periodontal Res."},{"key":"ref_8","first-page":"157","article-title":"The effect of resveratrol on expression of matrix metalloproteinase 9 and its tissue inhibitors in vascular smooth muscle cells","volume":"14","author":"Farrokhi","year":"2018","journal-title":"ARYA Atheroscler."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1016\/j.jnutbio.2005.01.017","article-title":"Resveratrol as an anticancer nutrient: Molecular basis, open questions and promises","volume":"16","author":"Signorelli","year":"2005","journal-title":"J. Nutr. Biochem."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1007\/s13105-018-0648-7","article-title":"Effects of resveratrol on regulation on UCP2 and cardiac function in diabetic rats","volume":"75","author":"Diao","year":"2019","journal-title":"J. Physiol. Biochem."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1002\/mnfr.200500022","article-title":"Resveratrol as an anti-inflammatory and anti-aging agent: Mechanisms and clinical implications","volume":"49","author":"Villegas","year":"2005","journal-title":"Mol. Nutr. Food. Res."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1041","DOI":"10.1089\/152308601317203567","article-title":"Biological effects of resveratrol","volume":"3","author":"Bhat","year":"2001","journal-title":"Antioxid. Redox Signal."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Hu, Y., Chen, D., Zheng, P., Yu, J., He, J., Mao, X., and Yu, B. (2019). The Bidirectional Interactions between Resveratrol and Gut Microbiota: An Insight into Oxidative Stress and Inflammatory Bowel Disease Therapy. Biomed. Res. Int., 2019.","DOI":"10.1155\/2019\/5403761"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2211","DOI":"10.1021\/jf803638d","article-title":"Effect of a low dose of dietary resveratrol on colon microbiota, inflammation and tissue damage in a DSS-induced colitis rat model","volume":"57","author":"Larrosa","year":"2009","journal-title":"J. Agric. Food. Chem."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Man, A.W.C., Li, H., and Xia, N. (2019). Resveratrol and the Interaction between Gut Microbiota and Arterial Remodelling. Nutrients, 12.","DOI":"10.3390\/nu12010119"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1002\/jcsm.12208","article-title":"Beta-hydroxy-beta-methylbutyrate supplementation and skeletal muscle in healthy and muscle-wasting conditions","volume":"8","year":"2017","journal-title":"J. Cachexia Sarcopenia Muscle"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1002\/jcsm.12032","article-title":"Conversion of leucine to \u03b2-hydroxy-\u03b2-methylbutyrate by \u03b1-keto isocaproate dioxygenase is required for a potent stimulation of protein synthesis in L6 rat myotubes","volume":"7","author":"Salto","year":"2016","journal-title":"J. Cachexia Sarcopenia Muscle"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Zhang, S., Tang, Z., Zheng, C., Zhong, Y., Zheng, J., Duan, G., Yin, Y., Duan, Y., and Song, Z. (2022). Dietary Beta-Hydroxy-Beta-Methyl Butyrate Supplementation Inhibits Hepatic Fat Deposition via Regulating Gut Microbiota in Broiler Chickens. Microorganisms, 10.","DOI":"10.3390\/microorganisms10010169"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"10019","DOI":"10.1096\/fj.201900665RR","article-title":"Gut microbiota mediates the protective effects of dietary \u03b2-hydroxy-\u03b2-methylbutyrate (HMB) against obesity induced by high-fat diets","volume":"33","author":"Duan","year":"2019","journal-title":"FASEB J."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1007\/s12603-017-0934-z","article-title":"Effects of Protein, Essential Amino Acids, B-Hydroxy B-Methylbutyrate, Creatine, Dehydroepiandrosterone and Fatty Acid Supplementation on Muscle Mass, Muscle Strength and Physical Performance in Older People Aged 60 Years and Over. A Systematic Review on the Literature","volume":"22","author":"Beaudart","year":"2018","journal-title":"J. Nutr. Health Aging"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Zhu, K.A., Zhang, Y., Zhang, F.S., Wu, Z.L., Su, Q., Hou, S.Z., and Gui, L.S. (2024). The Effects of Dietary Resveratrol and \u03b2-Hydroxy-\u03b2-Methylbutyric Acid Supplementation at Two Protein Levels on the Ruminal Microbiome and Metabolome of Tibetan Sheep. Agriculture, 14.","DOI":"10.3390\/agriculture14060936"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1016\/j.redox.2015.01.002","article-title":"Oxidative stress: A concept in redox biology and medicine","volume":"4","author":"Sies","year":"2015","journal-title":"Redox Biol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"8140962","DOI":"10.1155\/2017\/8140962","article-title":"The Evaluation of Antioxidant and Anti-Inflammatory Effects of Eucommia ulmoides Flavones Using Diquat-Challenged Piglet Models","volume":"2017","author":"Yuan","year":"2017","journal-title":"Oxid. Med. Cell. Longev."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"101886","DOI":"10.1016\/j.psj.2022.101886","article-title":"Dietary resveratrol improved production performance, egg quality, and intestinal health of laying hens under oxidative stress","volume":"101","author":"Ding","year":"2022","journal-title":"Poult. Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"101459","DOI":"10.1016\/j.psj.2021.101459","article-title":"Resveratrol sustains intestinal barrier integrity, improves antioxidant capacity, and alleviates inflammation in the jejunum of ducks exposed to acute heat stress","volume":"100","author":"Yang","year":"2021","journal-title":"Poult. Sci."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Arazi, H., Hosseini, Z., Asadi, A., Ramirez-Campillo, R., and Suzuki, K. (2019). \u03b2-Hydroxy-\u03b2-Methylbutyrate Free Acid Attenuates Oxidative Stress Induced by a Single Bout of Plyometric Exercise. Front. Physiol., 10.","DOI":"10.3389\/fphys.2019.00776"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1016\/j.jnutbio.2006.10.005","article-title":"The influence of antioxidant supplementation on markers of inflammation and the relationship to oxidative stress after exercise","volume":"18","author":"Peake","year":"2007","journal-title":"J. Nutr. Biochem."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"7432797","DOI":"10.1155\/2016\/7432797","article-title":"Oxidative Stress and Inflammation: What Polyphenols Can Do for Us?","volume":"2016","author":"Hussain","year":"2016","journal-title":"Oxid. Med. Cell. Longev."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1793","DOI":"10.1080\/10408398.2012.708368","article-title":"Effects of amino acid derivatives on physical, mental, and physiological activities","volume":"55","author":"Luckose","year":"2015","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1097\/MCO.0000000000000704","article-title":"Essential amino acid formulations to prevent mitochondrial dysfunction and oxidative stress","volume":"24","author":"Ruocco","year":"2021","journal-title":"Curr. Opin. Clin. Nutr. Metab. Care"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Gan, Z., Wei, W., Li, Y., Wu, J., Zhao, Y., Zhang, L., Wang, T., and Zhong, X. (2019). Curcumin and Resveratrol Regulate Intestinal Bacteria and Alleviate Intestinal Inflammation in Weaned Piglets. Molecules, 24.","DOI":"10.3390\/molecules24071220"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"5952707","DOI":"10.1155\/2018\/5952707","article-title":"Effect of Resveratrol Dry Suspension on Immune Function of Piglets","volume":"2018","author":"Fu","year":"2018","journal-title":"Evid. Based Complement. Altern. Med."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1016\/j.jevs.2013.03.064","article-title":"Identifying the role of a \u201ccaloric restriction mimetic\u201d, resveratrol, in Equine Metabolic Syndrome and its implications for targeted therapy","volume":"33","author":"Adams","year":"2013","journal-title":"J. Equine Vet. Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"353","DOI":"10.21873\/invivo.11481","article-title":"\u03b2-Hydroxy-\u03b2-methylbutyrate Suppresses NF-\u0138B Activation and IL-6 Production in TE-1 Cancer Cells","volume":"33","author":"Miyake","year":"2019","journal-title":"Vivo"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"8731","DOI":"10.1158\/0008-5472.CAN-04-1760","article-title":"Mechanism of the attenuation of proteolysis-inducing factor stimulated protein degradation in muscle by beta-hydroxy-beta-methylbutyrate","volume":"64","author":"Smith","year":"2004","journal-title":"Cancer Res."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Fraga, A.Z., Campos, P., Hauschild, L., Chalvon-Demersay, T., Beaumont, M., and Le Floc\u2019h, N. (2023). A blend of functional amino acids and grape polyphenols improves the pig capacity to cope with an inflammatory challenge caused by poor hygiene of housing conditions. BMC Vet. Res., 19.","DOI":"10.1186\/s12917-023-03580-w"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1032","DOI":"10.1038\/s41598-023-50852-4","article-title":"Effect of diet supplemented with functional amino acids and polyphenols on gut health in broilers subjected to a corticosterone-induced stress","volume":"14","author":"Yvon","year":"2024","journal-title":"Sci. Rep."},{"key":"ref_38","first-page":"625","article-title":"Effects of dietary resveratrol supplementation on digestive enzymes activities and serum biochemistry of rainbow trout (Oncorhynchus mykiss)","volume":"14","author":"Meshkini","year":"2023","journal-title":"Vet. Res. Forum."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Yang, S., Xu, W., Feng, L., Zhang, C., Yan, C., Zhang, J., Lai, J., Yan, T., He, Z., and Du, X. (2022). Resveratrol Improves the Digestive Ability and the Intestinal Health of Siberian Sturgeon. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms231911977"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"2343","DOI":"10.3382\/ps.2007-00110","article-title":"The effects of in ovo feeding arginine, beta-hydroxy-beta-methyl-butyrate, and protein on jejunal digestive and absorptive activity in embryonic and neonatal turkey poults","volume":"86","author":"Foye","year":"2007","journal-title":"Poult"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Zhang, J., Chai, X., Zhao, F., Hou, G., and Meng, Q. (2022). Food Applications and Potential Health Benefits of Hawthorn. Foods, 11.","DOI":"10.3390\/foods11182861"},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Kwon, O., Han, T.S., and Son, M.Y. (2020). Intestinal Morphogenesis in Development, Regeneration, and Disease: The Potential Utility of Intestinal Organoids for Studying Compartmentalization of the Crypt-Villus Structure. Front. Cell Dev. Biol., 8.","DOI":"10.3389\/fcell.2020.593969"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1757","DOI":"10.3382\/ps\/pex349","article-title":"Comparison of two methods for determination of intestinal villus to crypt ratios and documentation of early age-associated ratio changes in broiler chickens","volume":"97","author":"Wilson","year":"2018","journal-title":"Poult. Sci."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Gao, Y., Meng, L., Liu, H., Wang, J., and Zheng, N. (2020). The Compromised Intestinal Barrier Induced by Mycotoxins. Toxins, 12.","DOI":"10.3390\/toxins12100619"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1080\/10495398.2019.1683022","article-title":"Effects of dietary resveratrol supplementation on immunity, antioxidative capacity and intestinal barrier function in weaning piglets","volume":"32","author":"Chen","year":"2021","journal-title":"Anim. Biotechnol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1329","DOI":"10.1039\/C5FO01338K","article-title":"Resveratrol modulates intestinal morphology and HSP70\/90, NF-\u03baB and EGF expression in the jejunal mucosa of black-boned chickens on exposure to circular heat stress","volume":"7","author":"Liu","year":"2016","journal-title":"Food Funct."},{"key":"ref_47","first-page":"27","article-title":"Histological and biochemical evaluation of supplementing broiler diet with \u03b2-hydroxy-methyl butyrate calcium (\u03b2-HMB-Ca)","volume":"19","author":"Suad","year":"2018","journal-title":"Iran. J. Vet. Res."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"110839","DOI":"10.1016\/j.nut.2020.110839","article-title":"Dietary \u03b2-hydroxy-\u03b2-methylbutyrate improves intestinal function in weaned piglets after lipopolysaccharide challenge","volume":"78","author":"Zheng","year":"2020","journal-title":"Nutrition"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"15064","DOI":"10.1073\/pnas.0803124105","article-title":"The inner of the two Muc2 mucin-dependent mucus layers in colon is devoid of bacteria","volume":"105","author":"Johansson","year":"2008","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.carbpol.2015.07.070","article-title":"Immunomodulatory activity on macrophage of a purified polysaccharide extracted from Laminaria japonica","volume":"134","author":"Fang","year":"2015","journal-title":"Carbohydr. Polym."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Wang, P., Wang, J., Li, D., Ke, W., Chen, F., and Hu, X. (2020). Targeting the gut microbiota with resveratrol: A demonstration of novel evidence for the management of hepatic steatosis. J. Nutr. Biochem., 81.","DOI":"10.1016\/j.jnutbio.2020.108363"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"883","DOI":"10.1007\/s10571-019-00687-3","article-title":"Neuroprotection of Resveratrol Against Focal Cerebral Ischemia\/Reperfusion Injury in Mice Through a Mechanism Targeting Gut-Brain Axis","volume":"39","author":"Dou","year":"2019","journal-title":"Cell. Mol. Neurobiol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"11464","DOI":"10.1021\/acs.jafc.9b04442","article-title":"Methionine and Its Hydroxyl Analogues Improve Stem Cell Activity to Eliminate Deoxynivalenol-Induced Intestinal Injury by Reactivating Wnt\/\u03b2-Catenin Signaling","volume":"67","author":"Zhou","year":"2019","journal-title":"J. Agric. Food Chem."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1016\/j.fsi.2017.03.012","article-title":"Methionine hydroxy analogue improves intestinal immunological and physical barrier function in young grass carp (Ctenopharyngodon idella)","volume":"64","author":"Pan","year":"2017","journal-title":"Fish Shellfish Immunol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1186\/s12964-023-01219-9","article-title":"Short-chain fatty acids in diseases","volume":"21","author":"Zhang","year":"2023","journal-title":"Cell Commun. Signal."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1002\/JLB.3A1218-476RR","article-title":"Resveratrol modulates the gut microbiota to prevent murine colitis development through induction of Tregs and suppression of Th17 cells","volume":"106","author":"Alrafas","year":"2019","journal-title":"J. Leukoc. Biol."},{"key":"ref_57","first-page":"e266","article-title":"Digestibility, ruminal fermentation, blood metabolites and antioxidant status in ewes supplemented with DL-methionine or hydroxy-4 (methylthio) butanoic acid isopropyl ester","volume":"101","author":"Taghizadeh","year":"2017","journal-title":"J. Anim. Physiol. Anim. Nutr."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/j.clnu.2008.11.002","article-title":"Butyrate modulates oxidative stress in the colonic mucosa of healthy humans","volume":"28","author":"Hamer","year":"2009","journal-title":"Clin. Nutr."},{"key":"ref_59","first-page":"3745135","article-title":"Sodium Butyrate Ameliorates Oxidative Stress-Induced Intestinal Epithelium Barrier Injury and Mitochondrial Damage through AMPK-Mitophagy Pathway","volume":"2022","author":"Li","year":"2022","journal-title":"Oxid. Med. Cell. Longev."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"12181","DOI":"10.1039\/D1FO02116H","article-title":"A new paradigm for a new simple chemical: Butyrate & immune regulation","volume":"12","author":"Dang","year":"2021","journal-title":"Food Funct."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1016\/j.dld.2018.02.012","article-title":"Actinobacteria: A relevant minority for the maintenance of gut homeostasis","volume":"50","author":"Binda","year":"2018","journal-title":"Dig. Liver Dis."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"7365","DOI":"10.1007\/s00253-014-5954-6","article-title":"Marine actinobacteria associated with marine organisms and their potentials in producing pharmaceutical natural products","volume":"98","author":"Valliappan","year":"2014","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Frontiers Production Office (2020). Erratum: Microbiota of the Gut-Lymph Node Axis: Depletion of Mucosa-Associated Segmented Filamentous Bacteria and Enrichment of Methanobrevibacter by Colistin Sulfate and Linco-Spectin in Pigs. Front. Microbiol., 11.","DOI":"10.3389\/fmicb.2020.01051"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"E840","DOI":"10.1152\/ajpendo.00171.2015","article-title":"The short-chain fatty acid receptor, FFA2, contributes to gestational glucose homeostasis","volume":"309","author":"Fuller","year":"2015","journal-title":"Am. J. Physiol. Endocrinol. Metab."},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Yan, M., Yin, W., Fang, X., Guo, J., and Shi, H. (2016). Characteristics of a water-forming NADH oxidase from Methanobrevibacter smithii, an archaeon in the human gut. Biosci. Rep., 36.","DOI":"10.1042\/BSR20160357"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"2359500","DOI":"10.1080\/19490976.2024.2359500","article-title":"Archaea influence composition of endoscopically visible ileocolonic biofilms","volume":"16","author":"Orgler","year":"2024","journal-title":"Gut Microbes"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"102670","DOI":"10.1016\/j.psj.2023.102670","article-title":"Differential effects of Bacillus subtilis- and Bacillus licheniformis-fermented products on growth performance, intestinal morphology, intestinal antioxidant and barrier function gene expression, cecal microbiota community, and microbial carbohydrate-active enzyme composition in broilers","volume":"102","author":"Chen","year":"2023","journal-title":"Poult. Sci."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"101970","DOI":"10.1016\/j.psj.2022.101970","article-title":"Effect of Bacillus species-fermented products and essential oils on growth performance, gut morphology, cecal short-chain fatty acid levels, and microbiota community in broilers","volume":"101","author":"Chang","year":"2022","journal-title":"Poult. Sci."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"30","DOI":"10.2141\/jpsa.0200010","article-title":"Bacillus licheniformis-Fermented Products Improve Growth Performance and Intestinal Gut Morphology in Broilers under Clostridium perfringens Challenge","volume":"58","author":"Cheng","year":"2021","journal-title":"J. Poult. Sci."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"994","DOI":"10.2174\/1389200211314090006","article-title":"Butyrate and colorectal cancer: The role of butyrate transport","volume":"14","author":"Martel","year":"2013","journal-title":"Curr. Drug Metab."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/j.biochi.2022.09.009","article-title":"Mitochondrial peptide Mtln contributes to oxidative metabolism in mice","volume":"204","author":"Averina","year":"2023","journal-title":"Biochimie"},{"key":"ref_72","doi-asserted-by":"crossref","unstructured":"Liu, L., Zhang, W., Liu, T., Tan, Y., Chen, C., Zhao, J., Geng, H., and Ma, C. (2023). The physiological metabolite \u03b1-ketoglutarate ameliorates osteoarthritis by regulating mitophagy and oxidative stress. Redox Biol., 62.","DOI":"10.1016\/j.redox.2023.102663"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"100200","DOI":"10.1016\/j.advnut.2024.100200","article-title":"\u03b1-Ketoglutarate for Preventing and Managing Intestinal Epithelial Dysfunction","volume":"15","author":"Du","year":"2024","journal-title":"Adv. Nutr."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"13882","DOI":"10.1021\/acs.jafc.2c04641","article-title":"\u03b1-Ketoglutarate Restores Intestinal Barrier Function through Promoting Intestinal Stem Cells-Mediated Epithelial Regeneration in Colitis","volume":"70","author":"Si","year":"2022","journal-title":"J. Agric. Food Chem."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"e1700932","DOI":"10.1002\/mnfr.201700932","article-title":"Butyrate Inhibits Indices of Colorectal Carcinogenesis via Enhancing \u03b1-Ketoglutarate-Dependent DNA Demethylation of Mismatch Repair Genes","volume":"62","author":"Sun","year":"2018","journal-title":"Mol. Nutr. Food Res."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"1471","DOI":"10.1111\/j.1471-4159.1985.tb07214.x","article-title":"Succinic semialdehyde as a substrate for the formation of gamma-aminobutyric acid","volume":"45","author":"Schouten","year":"1985","journal-title":"J. Neurochem."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.phrs.2014.12.001","article-title":"GABA and GABA receptors in the gastrointestinal tract: From motility to inflammation","volume":"93","author":"Auteri","year":"2015","journal-title":"Pharmacol. Res."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/j.neuroscience.2019.05.064","article-title":"Reactive Oxygen Species in the Regulation of the GABA Mediated Inhibitory Neurotransmission","volume":"439","author":"Calvo","year":"2020","journal-title":"Neuroscience"},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"109056","DOI":"10.1016\/j.fsi.2023.109056","article-title":"Effect of dietary gamma aminobutyric acid (GABA) modulated the growth performance, immune and antioxidant capacity, digestive enzymes, intestinal histology and gene expression of Nile tilapia (Oreochromisniloticus)","volume":"141","author":"Ruenkoed","year":"2023","journal-title":"Fish Shellfish Immunol."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1007\/s00204-004-0602-z","article-title":"Role of diacetyl metabolite in alcohol toxicity and addiction via electron transfer and oxidative stress","volume":"79","author":"Kovacic","year":"2005","journal-title":"Arch. Toxicol."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1093\/toxsci\/kfn222","article-title":"Inhalation dosimetry of diacetyl and butyric acid, two components of butter flavoring vapors","volume":"108","author":"Morris","year":"2009","journal-title":"Toxicol. Sci."}],"container-title":["Antioxidants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-3921\/13\/8\/892\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:22:22Z","timestamp":1760109742000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-3921\/13\/8\/892"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7,24]]},"references-count":81,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2024,8]]}},"alternative-id":["antiox13080892"],"URL":"https:\/\/doi.org\/10.3390\/antiox13080892","relation":{},"ISSN":["2076-3921"],"issn-type":[{"value":"2076-3921","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,7,24]]}}}