{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T21:00:48Z","timestamp":1779224448966,"version":"3.51.4"},"reference-count":51,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2024,7,25]],"date-time":"2024-07-25T00:00:00Z","timestamp":1721865600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32372941"],"award-info":[{"award-number":["32372941"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["CAAS-ASTIP-2022-IAR"],"award-info":[{"award-number":["CAAS-ASTIP-2022-IAR"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Agricultural Science and Technology Innovation Program","award":["32372941"],"award-info":[{"award-number":["32372941"]}]},{"name":"Agricultural Science and Technology Innovation Program","award":["CAAS-ASTIP-2022-IAR"],"award-info":[{"award-number":["CAAS-ASTIP-2022-IAR"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>Lespedeza bicolor Turcz. (L. bicolor) honey, a monofloral honey, has garnered increased attention due to its origin in the L. bicolor plant. A previous study has shown that L. bicolor honey can ameliorate inflammation. In this study, we aimed to investigate the effects of L. bicolor honey extract and its biomarker (Trifolin) on DSS-induced ulcerative colitis (UC). Our results demonstrated that L. bicolor honey extract and Trifolin significantly increased the expression levels of the tight junction cytokines Claudin-1 and ZO-1. Additionally, they decreased the pro-inflammatory factors TNF-\u03b1 and IL-6 and enhanced the antioxidant factors NQO1 and GSTA1. Based on metabolomic analyses, L. bicolor honey extract and Trifolin regulated the progression of UC by inhibiting ferroptosis. Mechanistically, they improved the levels of SOD and iron load, increased the GSH\/GSSG ratio, reduced MDA content and ROS release, and upregulated the Nrf2\/HO-1 pathway, thereby inhibiting DSS-induced UC. Moreover, the expression levels of ferroptosis-related genes indicated that they decreased FTL, ACSL4, and PTGS2 while increasing SLC7A11 expression to resist ferroptosis. In conclusion, our study found that L. bicolor honey improves DSS-induced UC by inhibiting ferroptosis by activating the Nrf2\/HO-1 pathway. These findings further elucidate the understanding of anti-inflammatory and antioxidant activities of L. bicolor honey.<\/jats:p>","DOI":"10.3390\/antiox13080900","type":"journal-article","created":{"date-parts":[[2024,7,25]],"date-time":"2024-07-25T11:43:44Z","timestamp":1721907824000},"page":"900","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Lespedeza bicolor Turcz. Honey Prevents Inflammation Response and Inhibits Ferroptosis by Nrf2\/HO-1 Pathway in DSS-Induced Human Caco-2 Cells"],"prefix":"10.3390","volume":"13","author":[{"given":"Caijun","family":"Ren","sequence":"first","affiliation":[{"name":"State Key Laboratory of Resource Insects, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuying","family":"Zhu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Resource Insects, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5590-1744","authenticated-orcid":false,"given":"Qiangqiang","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Resource Insects, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miao","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Resource Insects, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suzhen","family":"Qi","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Resource Insects, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dandan","family":"Sun","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Resource Insects, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liming","family":"Wu","sequence":"additional","affiliation":[{"name":"Risk Assessment Laboratory for Bee Products Quality and Safety of Ministry of Agriculture, Beijing 100093, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liuwei","family":"Zhao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Resource Insects, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,7,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"135197","DOI":"10.1016\/j.foodchem.2022.135197","article-title":"Identification and characterization of plant-derived biomarkers and physicochemical variations in the maturation process of Triadica cochinchinensis honey based on UPLC-QTOF-MS metabolomics analysis","volume":"408","author":"Liu","year":"2023","journal-title":"Food Chem."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"135727","DOI":"10.1016\/j.foodchem.2023.135727","article-title":"Chemometric classification of chestnut honeys from different regions in Turkey based on their phenolic compositions and biological activities","volume":"415","author":"Ceylan","year":"2023","journal-title":"Food Chem."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"133229","DOI":"10.1016\/j.foodchem.2022.133229","article-title":"Honeys with anti-inflammatory capacity can alter the elderly gut microbiota in an ex vivo gut model","volume":"392","author":"Wu","year":"2022","journal-title":"Food Chem."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"138060","DOI":"10.1016\/j.foodchem.2023.138060","article-title":"An updated review of functional ingredients of Manuka honey and their value-added innovations","volume":"440","author":"Wang","year":"2024","journal-title":"Food Chem."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1016\/j.job.2022.08.001","article-title":"The protective effects of blueberry honey from Canada against H2O2-induced cytotoxicity in human buccal mucosal cells","volume":"64","author":"Hattori","year":"2022","journal-title":"J. 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