{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T06:55:20Z","timestamp":1784876120212,"version":"3.55.0"},"reference-count":53,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2024,7,29]],"date-time":"2024-07-29T00:00:00Z","timestamp":1722211200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Sichuan Department of Science and Technology Support Project","award":["2019YJ0650"],"award-info":[{"award-number":["2019YJ0650"]}]},{"name":"Sichuan Department of Science and Technology Support Project","award":["03572174"],"award-info":[{"award-number":["03572174"]}]},{"name":"Sichuan Department of Science and Technology Support Project","award":["NSF2021ZR01"],"award-info":[{"award-number":["NSF2021ZR01"]}]},{"name":"Double Support Project for Discipline Construction of Sichuan Agricultural University","award":["2019YJ0650"],"award-info":[{"award-number":["2019YJ0650"]}]},{"name":"Double Support Project for Discipline Construction of Sichuan Agricultural University","award":["03572174"],"award-info":[{"award-number":["03572174"]}]},{"name":"Double Support Project for Discipline Construction of Sichuan Agricultural University","award":["NSF2021ZR01"],"award-info":[{"award-number":["NSF2021ZR01"]}]},{"name":"Key Technology Innovation Support Project for Industrial Development of Jiangsu Agri-animal Husbandry Vocational College","award":["2019YJ0650"],"award-info":[{"award-number":["2019YJ0650"]}]},{"name":"Key Technology Innovation Support Project for Industrial Development of Jiangsu Agri-animal Husbandry Vocational College","award":["03572174"],"award-info":[{"award-number":["03572174"]}]},{"name":"Key Technology Innovation Support Project for Industrial Development of Jiangsu Agri-animal Husbandry Vocational College","award":["NSF2021ZR01"],"award-info":[{"award-number":["NSF2021ZR01"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>Our preliminary study identified dairy cow placenta extract (CPE) as a mixture of peptides with potent antioxidant activity both in vivo and in vitro. However, the specific antioxidant peptides (AOPs) responsible for this activity were not yet identified. In the current study, we employed virtual screening and chromatography techniques to isolate two peptides, ANNGKQWAEVF (CP1) and QPGLPGPAG (CP2), from CPE. These peptides were found to be less stable under extreme conditions such as high temperature, strong acid, strong alkali, and simulated digestive conditions. Nevertheless, under normal physiological conditions, both CP1 and CP2 exhibited significant antioxidant properties, including free-radical scavenging, metal chelating, and the inhibition of lipid peroxidation. They also up-regulated the activities of intracellular antioxidant enzymes in response to hydrogen-peroxide-induced oxidative stress, resulting in reduced MDA levels, a decreased expression of the Keap1 gene and protein, and increased levels of the Nrf2 and HO-1 genes and proteins. Furthermore, CP1 demonstrated superior antioxidant activity compared to CP2. These findings suggest that CP1 and CP2 hold potential for mitigating oxidative stress in vitro and highlight the efficacy of virtual screening as a method for isolating AOPs within CPE.<\/jats:p>","DOI":"10.3390\/antiox13080913","type":"journal-article","created":{"date-parts":[[2024,8,1]],"date-time":"2024-08-01T12:38:20Z","timestamp":1722515900000},"page":"913","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Isolation and Characterization of Antioxidant Peptides from Dairy Cow (Bos taurus) Placenta and Their Antioxidant Activities"],"prefix":"10.3390","volume":"13","author":[{"given":"Xinyu","family":"Tian","sequence":"first","affiliation":[{"name":"The Key Laboratory of Animal Disease and Human Health of Sichuan Province, The Medical Research Center for Cow Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, 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Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi","family":"Zeng","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Animal Disease and Human Health of Sichuan Province, The Medical Research Center for Cow Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weijian","family":"Zheng","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Animal Disease and Human Health of Sichuan Province, The Medical Research Center for Cow Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanwen","family":"Zhang","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Animal Disease and Human Health of Sichuan Province, The Medical Research Center for Cow Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuxin","family":"Luo","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Animal Disease and Human Health of Sichuan Province, The Medical Research Center for Cow Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Lu","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Jiangsu Agri-Animal Husbandry Vocational College, Taizhou 225300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7181-7603","authenticated-orcid":false,"given":"Lei","family":"Fan","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Animal Disease and Human Health of Sichuan Province, The Medical Research Center for Cow Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"},{"name":"Department of Pharmacology, School of Basic Medical Sciences, Wuhan University, Wuhan 430071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2832-5806","authenticated-orcid":false,"given":"Liuhong","family":"Shen","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Animal Disease and Human Health of Sichuan Province, The Medical Research Center for Cow Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,7,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"103236","DOI":"10.1016\/j.fbio.2023.103236","article-title":"Inhibitory effects and action mechanism of five antioxidant peptides derived from wheat gluten on cells oxidative stress 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