{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T18:11:36Z","timestamp":1790878296646,"version":"4.1.0"},"reference-count":33,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2024,7,29]],"date-time":"2024-07-29T00:00:00Z","timestamp":1722211200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Science and Technology Council, Taiwan","award":["NSTC 112-2320-B-A49-028-MY3"],"award-info":[{"award-number":["NSTC 112-2320-B-A49-028-MY3"]}]},{"name":"National Science and Technology Council, Taiwan","award":["MOST 109-2320-B-010-029-MY3"],"award-info":[{"award-number":["MOST 109-2320-B-010-029-MY3"]}]},{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology, Taiwan","doi-asserted-by":"publisher","award":["NSTC 112-2320-B-A49-028-MY3"],"award-info":[{"award-number":["NSTC 112-2320-B-A49-028-MY3"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology, Taiwan","doi-asserted-by":"publisher","award":["MOST 109-2320-B-010-029-MY3"],"award-info":[{"award-number":["MOST 109-2320-B-010-029-MY3"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>Dendrobium fimbriatum is a perennial herb, and its stems are high-grade tea and nourishing medicinal materials. Various solvent extracts of D. fimbriatum were evaluated for their anti-inflammatory, anti-acetylcholinesterase (AChE), antioxidant, and anti-\u03b1-glucosidase properties. Acetone and EtOAc extracts showed significant antioxidant effects. Acetone, n-hexane, and EtOAc extracts revealed potent inhibition against \u03b1-glucosidase. EtOAc, n-hexane, and dichloromethane extracts displayed significant anti-AChE activity. Among the isolated constituents, gigantol, moscatin, and dendrophenol showed potent antioxidant activities in FRAP, DPPH, and ABTS radical scavenging tests. Moscatin (IC50 = 161.86 \u00b1 16.45 \u03bcM) and dendrophenol (IC50 = 165.19 \u00b1 13.25 \u03bcM) displayed more potent anti-AChE activity than chlorogenic acid (IC50 = 236.24 \u00b1 15.85 \u03bcM, positive control). Dendrophenol (IC50 = 14.31 \u00b1 3.17 \u03bcM) revealed more efficient anti-NO activity than quercetin (positive control, IC50 = 23.09 \u00b1 1.43 \u03bcM). Analysis of AChE and iNOS inhibitory components was performed using molecular docking and\/or the bioaffinity ultrafiltration method. In bioaffinity ultrafiltration, the binding affinity of compounds to the enzyme (acetylcholinesterase and inducible nitric oxide synthase) was determined using the enrichment factor (EF). Among the main components of the EtOAc extract from D. fimbriatum stem, moscatin, dendrophenol, gigantol, and batatasin III with acetylcholinesterase exhibited the highest binding affinities, with affinity values of 66.31%, 59.48%, 54.60%, and 31.87%, respectively. Moreover, the affinity capacity of the identified compounds with inducible nitric oxide synthase can be ranked as moscatin (88.99%) &gt; dendrophenol (65.11%) &gt; gigantol (44.84%) &gt; batatasin III (27.18%). This research suggests that the bioactive extracts and components of D. fimbriatum stem could be studied further as hopeful candidates for the prevention or treatment of hyperglycemia, oxidative stress-related diseases, and nervous disorders.<\/jats:p>","DOI":"10.3390\/antiox13080918","type":"journal-article","created":{"date-parts":[[2024,8,1]],"date-time":"2024-08-01T12:38:20Z","timestamp":1722515900000},"page":"918","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Bioaffinity Ultrafiltration Combined with HPLC-ESI-qTOF-MS\/MS for Screening Potential Bioactive Components from the Stems of Dendrobium fimbriatum and In Silico Analysis"],"prefix":"10.3390","volume":"13","author":[{"given":"Yu-Hui","family":"Hsieh","sequence":"first","affiliation":[{"name":"Biomedical Industry Ph.D. Program, School of Life Sciences, National Yang Ming Chiao Tung University, Taipei 112304, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wu-Chang","family":"Chuang","sequence":"additional","affiliation":[{"name":"Brion Research Institute of Taiwan, New Taipei City 231030, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming-Chung","family":"Lee","sequence":"additional","affiliation":[{"name":"Sun Ten Pharmaceutical Co., Ltd., New Taipei City 231030, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Hsin","family":"Fan","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, School of Pharmaceutical Sciences, National Yang Ming Chiao Tung University, Taipei 112304, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9502-1856","authenticated-orcid":false,"given":"Nai-Kuei","family":"Huang","sequence":"additional","affiliation":[{"name":"National Research Institute of Chinese Medicine, Ministry of Health and Welfare, Taipei 112304, Taiwan"},{"name":"Ph.D. Program for Neural Regenerative Medicine, College of Medical Science and Technology, Taipei Medical University, Taipei 110301, Taiwan"},{"name":"Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei 110301, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5680-9533","authenticated-orcid":false,"given":"Jih-Jung","family":"Chen","sequence":"additional","affiliation":[{"name":"Biomedical Industry Ph.D. Program, School of Life Sciences, National Yang Ming Chiao Tung University, Taipei 112304, Taiwan"},{"name":"Department of Pharmacy, School of Pharmaceutical Sciences, National Yang Ming Chiao Tung University, Taipei 112304, Taiwan"},{"name":"Department of Medical Research, China Medical University Hospital, China Medical University, Taichung 404333, Taiwan"},{"name":"Traditional Herbal Medicine Research Center, Taipei Medical University Hospital, Taipei 110301, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,7,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"881","DOI":"10.1021\/acs.jafc.5b05180","article-title":"Structure characterization and immunomodulating effects of polysaccharides isolated from Dendrobium officinale","volume":"64","author":"Wei","year":"2016","journal-title":"J. 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