{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T07:13:01Z","timestamp":1787987581621,"version":"build-2784847793"},"reference-count":37,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2024,9,6]],"date-time":"2024-09-06T00:00:00Z","timestamp":1725580800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Agricultural Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences","award":["CAAS-ASTIP-2023-IFR-12"],"award-info":[{"award-number":["CAAS-ASTIP-2023-IFR-12"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>The placenta plays a crucial role in nutrient transport and waste exchange between the dam and fetus, sustaining fetal growth. While the positive effects of 25-hydroxyvitamin D3 (25-OH-D3) on animal performance have been reported, its impact on placental function remains largely unknown. Therefore, this study aimed to investigate the effects of supplementing 25-OH-D3 in the diet of primiparous sows on reproductive performance, antioxidant capacity, placental oxidative stress, nutrient transport, and inflammatory response during mid-to-late gestation. A total of 45 healthy Landrace \u00d7 Yorkshire primiparous sows on day 60 of gestation were selected and randomly allocated to three treatment groups based on body weight and backfat thickness: the control group (corn-soybean meal basal diet), the VD3 group (basal diet + 2000 IU VD3), and the 25-OH-D3 group (basal diet + 50 \u03bcg\/kg 25-OH-D3). The results demonstrated that supplementation with 25-OH-D3 in the diet enhanced sows\u2019 average litter weight and birth weight during mid-to-late gestation. Additionally, plasma malondialdehyde (MDA) concentrations in sows significantly decreased in the VD3 and 25-OH-D3 groups (p &lt; 0.05). Furthermore, lower gene expressions of placental HO-1, GPX2, IL-8, and IL-6 were found in the VD3 or 25-OH-D3 groups (p &lt; 0.05 or p &lt; 0.10), while higher gene expressions of GLUT1 and SNAT2 in the placenta of sows were observed in the VD3 and 25-OH-D3 groups, respectively (p &lt; 0.05). These findings indicate that the supplementation of VD3 and 25-OH-D3 in the diet of sows can improve their plasma oxidative stress status, enhance placental antioxidant capacity and nutrient transport, and reduce placental inflammatory responses, with more pronounced improvements in sow performance observed in sows fed diets supplemented with 25-OH-D3.<\/jats:p>","DOI":"10.3390\/antiox13091090","type":"journal-article","created":{"date-parts":[[2024,9,6]],"date-time":"2024-09-06T07:42:06Z","timestamp":1725608526000},"page":"1090","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Dietary Supplementation with 25-Hydroxyvitamin D3 on Reproductive Performance and Placental Oxidative Stress in Primiparous Sows during Mid-to-Late Gestation"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0089-545X","authenticated-orcid":false,"given":"Jing","family":"Li","sequence":"first","affiliation":[{"name":"Key Laboratory of Feed Biotechnology of Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingyue","family":"Bi","sequence":"additional","affiliation":[{"name":"Key Laboratory of Feed Biotechnology of Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing 100081, China"},{"name":"College of Agriculture, Yanbian University, Yanji 133000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1320-0290","authenticated-orcid":false,"given":"Yu","family":"Pi","sequence":"additional","affiliation":[{"name":"Key Laboratory of Feed Biotechnology of Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianren","family":"Jiang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Feed Biotechnology of Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanpin","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Feed Biotechnology of Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xilong","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Feed Biotechnology of Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,9,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Ampode, K.M.B., Mun, H.S., Lagua, E.B., Chem, V., Park, H.R., Kim, Y.H., and Yang, C.J. (2023). Bump Feeding Improves Sow Reproductive Performance, Milk Yield, Piglet Birth Weight, and Farrowing Behavior. Animals, 13.","DOI":"10.3390\/ani13193148"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1680","DOI":"10.1016\/j.cell.2020.05.002","article-title":"Metabolic Dynamics and Prediction of Gestational Age and Time to Delivery in Pregnant Women","volume":"181","author":"Liang","year":"2020","journal-title":"Cell"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Yang, X., Hu, R., Shi, M., Wang, L., Yan, J., Gong, J., Zhang, Q., He, J., and Wu, S. (2023). Placental Malfunction, Fetal Survival and Development Caused by Sow Metabolic Disorder: The Impact of Maternal Oxidative Stress. Antioxidants, 12.","DOI":"10.3390\/antiox12020360"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.ejmech.2015.04.040","article-title":"The role of antioxidants in the chemistry of oxidative stress: A review","volume":"97","author":"Pisoschi","year":"2015","journal-title":"Eur. J. Med. Chem."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"722","DOI":"10.5713\/ajas.19.0334","article-title":"Oxidative stress status and reproductive performance of sows during gestation and lactation under different thermal environments","volume":"33","author":"Zhao","year":"2020","journal-title":"Asian-Australas. J. Anim."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1774","DOI":"10.1017\/S1751731111000772","article-title":"Oxidative stress status of highly prolific sows during gestation and lactation","volume":"5","author":"Kim","year":"2011","journal-title":"Animal"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Xie, C., Wu, X., Long, C., Wang, Q., Fan, Z., Li, S., and Yin, Y. (2016). Chitosan oligosaccharide affects antioxidant defense capacity and placental amino acids transport of sows. BMC Vet. Res., 12.","DOI":"10.1186\/s12917-016-0872-8"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1007\/s00404-002-0302-y","article-title":"Lipid and protein oxidation and antioxidant function in women with mild and severe preeclampsia","volume":"268","author":"Serdar","year":"2003","journal-title":"Arch. Gynecol. Obstet."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1007\/s00418-004-0677-x","article-title":"Oxidative stress in the placenta","volume":"122","author":"Myatt","year":"2004","journal-title":"Histochem. Cell"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"101220","DOI":"10.1016\/j.psj.2021.101220","article-title":"Effects of the in ovo injection of vitamin D(3) and 25-hydroxyvitamin D(3) in Ross 708 broilers subsequently fed commercial or calcium and phosphorus-restricted diets. I. Performance, carcass characteristics, and incidence of woody breast myopathy(1,2,3)","volume":"100","author":"Fatemi","year":"2021","journal-title":"Poultry Sci."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Zhan, D., Zhao, J., Shi, Q., Lou, J., and Wang, W. (2023). 25-hydroxyvitamin D3 inhibits oxidative stress and ferroptosis in retinal microvascular endothelial cells induced by high glucose through down-regulation of miR-93. BMC Ophthalmol., 23.","DOI":"10.1186\/s12886-022-02762-8"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/j.ecl.2013.09.010","article-title":"Vitamin D and diabetes","volume":"43","author":"Mitri","year":"2014","journal-title":"Endo. Crin. Metab. Clin."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1007\/s00223-012-9619-0","article-title":"Molecular mechanisms of vitamin D action","volume":"92","author":"Haussler","year":"2013","journal-title":"Calcified Tissue Int."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"555","DOI":"10.4103\/ijem.IJEM_140_17","article-title":"Effect of vitamin D supplementation on vascular functions and oxidative stress in type 2 diabetic patients with vitamin D deficiency","volume":"21","author":"Anandabaskar","year":"2017","journal-title":"Indian J. Endocrinol. Metab."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"476","DOI":"10.1111\/asj.12638","article-title":"Effects of 25-hydroxycholecalciferol supplementation in maternal diets on milk quality and serum bone status markers of sows and bone quality of piglets","volume":"88","author":"Zhou","year":"2017","journal-title":"Anim. Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1288","DOI":"10.1111\/jpn.13768","article-title":"Dietary supplementation of 25-hydroxycholecalciferol as an alternative to cholecalciferol in swine diets: A review","volume":"106","author":"Schulz","year":"2022","journal-title":"J. Anim. Physiol. An. Nutri"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s40813-016-0049-7","article-title":"Factors for improving reproductive performance of sows and herd productivity in commercial breeding herds","volume":"3","author":"Koketsu","year":"2017","journal-title":"Porcine Health Manag."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"899","DOI":"10.2527\/jas.2013-7209","article-title":"Triennial Growth Symposium--Effects of dietary 25-hydroxycholecalciferol and cholecalciferol on blood vitamin D and mineral status, bone turnover, milk composition, and reproductive performance of sows","volume":"92","author":"Weber","year":"2014","journal-title":"J. Anim. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"e13391","DOI":"10.1111\/asj.13391","article-title":"Effects of 25-hydroxycholecalciferol supplementation in maternal diets on reproductive performance and the expression of genes that regulate lactation in sows","volume":"91","author":"Wang","year":"2020","journal-title":"Anim. Sci. J."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"114732","DOI":"10.1016\/j.anifeedsci.2020.114732","article-title":"Effects of dietary supplementation with 25-OH-D3 during gestation and lactation on reproduction, sow characteristics and piglet performance to weaning: 25-hydroxyvitamin D3 in sows","volume":"271","author":"Upadhaya","year":"2021","journal-title":"Anim. Feed Sci. Tech."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"202","DOI":"10.2527\/jas.2009-1976","article-title":"Reproductive performance and bone status markers of gilts and lactating sows supplemented with two different forms of vitamin D1","volume":"88","author":"Lauridsen","year":"2010","journal-title":"J. Anim. Sci."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Zhang, L., Wang, J., and Piao, X. (2022). Potential Effects of 25-Hydroxycholecalciferol on the Growth Performance, Blood Antioxidant Capacity, Intestinal Barrier Function and Microbiota in Broilers under Lipopolysaccharide Challenge. Antioxidants, 11.","DOI":"10.3390\/antiox11112094"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Zhou, X., Zou, Y., Xu, Y., Zhang, Z., Wu, Y., Cao, J., Qiu, B., Qin, X., Han, D., and Piao, X. (2022). Dietary supplementation of 25-hydroxyvitamin D3 improves growth performance, antioxidant capacity and immune function in weaned piglets. Antioxidants, 11.","DOI":"10.3390\/antiox11091750"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1080\/1745039X.2020.1822710","article-title":"Effects of maternal 25-hydroxycholecalciferol during the last week of gestation and lactation on serum parameters, intestinal morphology and microbiota in suckling piglets","volume":"74","author":"Zhang","year":"2020","journal-title":"Arch. Anim. Nutr."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1201","DOI":"10.1080\/1828051X.2021.1961616","article-title":"Maternal cholecalciferol supplementation during gestation improves antioxidant capacities in gilts and piglets","volume":"20","author":"Zhao","year":"2021","journal-title":"Italian J. Anim. Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1054797","DOI":"10.1155\/2018\/1054797","article-title":"The Tryptophan Pathway Targeting Antioxidant Capacity in the Placenta","volume":"2018","author":"Xu","year":"2018","journal-title":"Oxid. Med. Cell Longev."},{"key":"ref_28","first-page":"150","article-title":"Malondialdehyde and Antioxidant Enzymes in Maternal and Cord Blood, and their Correlation in Normotensive and Preeclamptic Women","volume":"1","author":"Suhail","year":"2009","journal-title":"J. Clin. Med. Res."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.mod.2009.09.004","article-title":"Genes and signals regulating murine trophoblast cell development","volume":"127","author":"Warburton","year":"2010","journal-title":"Mech. Develop."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1146\/annurev-pharmtox-011112-140320","article-title":"Role of nrf2 in oxidative stress and toxicity","volume":"53","author":"Ma","year":"2013","journal-title":"Annu. Rev. Pharmacol. Toxicol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1016\/j.freeradbiomed.2015.05.036","article-title":"Oxidative stress response and Nrf2 signaling in aging","volume":"88","author":"Zhang","year":"2015","journal-title":"Free. Radic. Bio. Med."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1067","DOI":"10.1038\/s12276-022-00829-6","article-title":"The Nrf2 antioxidant defense system in intervertebral disc degeneration: Molecular insights","volume":"54","author":"Xiang","year":"2022","journal-title":"Exp. Mol. Med."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1016\/j.placenta.2007.05.002","article-title":"Regulation of Placental Nutrient Transport\u2014A Review","volume":"28","author":"Jones","year":"2007","journal-title":"Placenta"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"179827","DOI":"10.1155\/2012\/179827","article-title":"Regulation of Nutrient Transport across the Placenta","volume":"2012","author":"Lager","year":"2012","journal-title":"J. Pregnancy"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1575","DOI":"10.1016\/S0002-9378(96)70609-9","article-title":"Maternal concentrations and fetal-maternal concentration differences of plasma amino acids in normal and intrauterine growth-restricted pregnancies","volume":"174","author":"Cetin","year":"1996","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"5427","DOI":"10.1210\/jcem.86.11.8036","article-title":"Placental transport of leucine, phenylalanine, glycine, and proline in intrauterine growth-restricted pregnancies","volume":"86","author":"Paolini","year":"2001","journal-title":"J. Clin. Endocr. Metab."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"2049","DOI":"10.1042\/CS20210575","article-title":"Placenta-specific Slc38a2\/SNAT2 knockdown causes fetal growth restriction in mice","volume":"135","author":"Maksym","year":"2021","journal-title":"Clin. Sci."}],"container-title":["Antioxidants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-3921\/13\/9\/1090\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:49:47Z","timestamp":1760111387000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-3921\/13\/9\/1090"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9,6]]},"references-count":37,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2024,9]]}},"alternative-id":["antiox13091090"],"URL":"https:\/\/doi.org\/10.3390\/antiox13091090","relation":{},"ISSN":["2076-3921"],"issn-type":[{"value":"2076-3921","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,9,6]]}}}