{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,11]],"date-time":"2026-11-11T01:17:09Z","timestamp":1794359829455,"version":"4.9.0"},"reference-count":38,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2024,12,17]],"date-time":"2024-12-17T00:00:00Z","timestamp":1734393600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Institute of Fisheries Science, Ministry of Oceans and Fisheries, Republic of Korea","award":["R2024019"],"award-info":[{"award-number":["R2024019"]}],"id":[{"id":"https:\/\/ror.org\/02chzeh21","id-type":"ROR","asserted-by":"crossref"}]},{"name":"National Institute of Fisheries Science, Ministry of Oceans and Fisheries, Republic of Korea","award":["RS-2021-NR060118"],"award-info":[{"award-number":["RS-2021-NR060118"]}],"id":[{"id":"https:\/\/ror.org\/02chzeh21","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Ministry of Education","award":["R2024019"],"award-info":[{"award-number":["R2024019"]}]},{"name":"Ministry of Education","award":["RS-2021-NR060118"],"award-info":[{"award-number":["RS-2021-NR060118"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>Melanogenesis, the biological process responsible for melanin synthesis, plays a crucial role in determining skin and hair color, photoprotection, and serving as a biomarker in various diseases. While various factors regulate melanogenesis, the role of fatty acids in this process remains underexplored. This study investigated the anti-melanogenic properties of 10(E)-pentadecenoic acid (10E-PDA) through both in silico and in vitro analyses. SwissSimilarity was utilized to predict the functional properties of 10E-PDA by comparing it with structurally similar lipids known to exhibit anti-melanogenic effects. Subsequent in vitro experiments demonstrated that 10E-PDA significantly reduced melanin production and intracellular tyrosinase activity in \u03b1-MSH (melanocyte-stimulating hormone)-stimulated B16F10 melanoma cells without exhibiting significant cytotoxicity at concentrations up to 15 \u03bcM. Further mechanistic studies revealed that 10E-PDA inhibited the nuclear translocation of microphthalmia-associated transcription factor (MITF), consistent with the decrease observed in p-MITF protein levels. It also decreased the mRNA levels of tyrosinase-related proteins (TRP-1, TRP-2) and tyrosinase, while reducing the protein levels of TRP-1 and tyrosinase, but not TRP-2. These findings suggest that 10E-PDA exerts its anti-melanogenic effects by modulating the MITF\/tyrosinase axis, presenting potential therapeutic implications for skin pigmentation disorders.<\/jats:p>","DOI":"10.3390\/antiox13121547","type":"journal-article","created":{"date-parts":[[2024,12,17]],"date-time":"2024-12-17T05:26:02Z","timestamp":1734413162000},"page":"1547","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["10(E)-Pentadecenoic Acid Inhibits Melanogenesis Partly Through Suppressing the Intracellular MITF\/Tyrosinase Axis"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7292-8743","authenticated-orcid":false,"given":"Min-Kyeong","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Food Science and Nutrition, Pukyong National University, Busan 48513, Republic of Korea"},{"name":"Biotechnology Research Division, National Institute of Fisheries Science, Busan 46083, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyoung Mi","family":"Moon","sequence":"additional","affiliation":[{"name":"Department of Food Science and Nutrition, Pukyong National University, Busan 48513, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-0789-5935","authenticated-orcid":false,"given":"Su-Yeon","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Smart Green Technology Engineering, Pukyong National University, Busan 48513, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaeseong","family":"Seo","sequence":"additional","affiliation":[{"name":"Department of Food Science and Nutrition, Pukyong National University, Busan 48513, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ah-Reum","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Smart Green Technology Engineering, Pukyong National University, Busan 48513, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bonggi","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Food Science and Nutrition, Pukyong National University, Busan 48513, Republic of Korea"},{"name":"Department of Smart Green Technology Engineering, Pukyong National University, Busan 48513, Republic of Korea"},{"name":"Marine Integrated Biomedical Technology Center, The National Key Research Institutes, Pukyong National University, Busan 48513, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,12,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1111\/pcmr.13165","article-title":"The metabolism of melanin synthesis\u2014From melanocytes to melanoma","volume":"37","author":"Snyman","year":"2024","journal-title":"Pigment. 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