{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T01:25:12Z","timestamp":1785461112515,"version":"3.56.0"},"reference-count":64,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2025,1,10]],"date-time":"2025-01-10T00:00:00Z","timestamp":1736467200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"ATL ADVANCED TECHNOLOGIES LABORATORIES"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>Sperm cryopreservation provides patients undergoing oncological, surgical, or infertility treatments the opportunity to conceive their own children, using assisted reproductive technologies. However, the freezing-thawing process can negatively influence both the quantity and the quality of spermatozoa, mainly due to an excessive production of reactive oxygen species and\/or an impaired antioxidant defense system in sperm. Aromatic and medicinal plants synthesize essential oils with antioxidant proprieties as a part of their ecological adaptation to environmental stress, thanks to their rich bioactive phytochemical components. This study aimed to assess sperm progressive motility, viability, plasma membrane functionality, and lipid peroxidation levels of human cryopreserved normozoospermic (n = 51) and asthenozoospermic (n = 51) samples without or with the addition of Thymus satureoides (TSEO) (20 \u00b5g\/mL), Artemisia vulgaris (AVEO) (48 \u00b5g\/mL), and Rosmarinus officinalis (ROEO) (13 \u00b5g\/mL) essential oils. Sperm parameters were significantly better preserved with ROEO in both normozoospermic (p &lt; 0.05) and asthenozoospermic samples (p &lt; 0.01). In contrast, TSEO had a negative impact for both groups (p &lt; 0.05). Meanwhile, no significant effects were observed with AVEO. In summary, the study revealed that in vitro addition of essential oils as antioxidant agents during cryopreservation can be either beneficial, which helps preserve sperm parameters and fertilizing potential, or detrimental as spermicidal agents.<\/jats:p>","DOI":"10.3390\/antiox14010075","type":"journal-article","created":{"date-parts":[[2025,1,10]],"date-time":"2025-01-10T06:25:54Z","timestamp":1736490354000},"page":"75","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Effects of Essential Oils as Antioxidant and Cryoprotective Agents in Improving Frozen and Thawed Human Sperm Criteria"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1779-9341","authenticated-orcid":false,"given":"Hamza","family":"Goujet","sequence":"first","affiliation":[{"name":"Laboratory of Physiopathology and Molecular Genetics, Ben M\u2019Sik Faculty of Sciences, Hassan II University of Casablanca, Casablanca 20670, Morocco"},{"name":"Reproductive Medicine, Reproductive Biology and Genetics, Peritox Laboratory, University Hospital and School of Medicine, Picardie University Jules Verne, 80054 Amiens, France"},{"name":"Laboratory of Medical Analyses, Reproductive Biology, LABOMAC, IRIFIV Centre, Casablanca 20100, Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ismail","family":"Kaarouch","sequence":"additional","affiliation":[{"name":"African Fertility Center, Private Clinic of Human Reproduction and Endoscopic Surgery, Casablanca 20000, Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abderrahim","family":"Malki","sequence":"additional","affiliation":[{"name":"Laboratory of Physiopathology and Molecular Genetics, Ben M\u2019Sik Faculty of Sciences, Hassan II University of Casablanca, Casablanca 20670, Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0742-8080","authenticated-orcid":false,"given":"Modou Mamoune","family":"Mbaye","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Analyses, Reproductive Biology, LABOMAC, IRIFIV Centre, Casablanca 20100, Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosalie","family":"Cabry","sequence":"additional","affiliation":[{"name":"Reproductive Medicine, Reproductive Biology and Genetics, Peritox Laboratory, University Hospital and School of Medicine, Picardie University Jules Verne, 80054 Amiens, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noureddine","family":"Louanjli","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Analyses, Reproductive Biology, LABOMAC, IRIFIV Centre, Casablanca 20100, Morocco"},{"name":"African Fertility Center, Private Clinic of Human Reproduction and Endoscopic Surgery, Casablanca 20000, Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taha","family":"Rhouda","sequence":"additional","affiliation":[{"name":"Laboratory of Physiopathology and Molecular Genetics, Ben M\u2019Sik Faculty of Sciences, Hassan II University of Casablanca, Casablanca 20670, Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moncef","family":"Benkhalifa","sequence":"additional","affiliation":[{"name":"Reproductive Medicine, Reproductive Biology and Genetics, Peritox Laboratory, University Hospital and School of Medicine, Picardie University Jules Verne, 80054 Amiens, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,1,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1093\/humupd\/dmp015","article-title":"Psychological Aspects of Fertility Preservation in Men and Women Affected by Cancer and Other Life-Threatening Diseases","volume":"15","author":"Tschudin","year":"2009","journal-title":"Hum. 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