{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T21:10:44Z","timestamp":1784754644726,"version":"3.55.0"},"reference-count":59,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2025,2,13]],"date-time":"2025-02-13T00:00:00Z","timestamp":1739404800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Sichuan Provincial Science and Technology Project","award":["2024JDHJ0015"],"award-info":[{"award-number":["2024JDHJ0015"]}]},{"name":"the Sichuan Provincial Science and Technology Project","award":["232102520016"],"award-info":[{"award-number":["232102520016"]}]},{"name":"the Projects of International Cooperation of Henan Province","award":["2024JDHJ0015"],"award-info":[{"award-number":["2024JDHJ0015"]}]},{"name":"the Projects of International Cooperation of Henan Province","award":["232102520016"],"award-info":[{"award-number":["232102520016"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>Aging is associated with detrimental bone loss, often leading to fragility fractures, which may be driven by oxidative stress. In this study, the outcomes of comparing the differences among young, adult and aged C57BL\/6J mice found that the trabecular bone volume was significantly lower in the aged mice compared to young mice, and the bone characteristics were significantly correlated with the oxidative status. To counteract the adverse effects of aging, methyl sulfonyl methane (MSM), a stable metabolite of dimethyl sulfoxide, was used to supplement the drinking water (400 mg\/kg\/day) of the aged mice (73 weeks old) for 8 weeks. The MSM supplementation improved the maximum load, bone microarchitecture, and mRNA levels of osteocyte-specific genes in the tibia. Furthermore, MSM reduced the serum level of the C-terminal telopeptide of type I collagen, a marker of bone resorption, and downregulated the mRNA levels of genes related to osteoclast proliferation and activity. MSM also decreased the levels of pro-inflammatory cytokines in both the serum and bone marrow. Importantly, the MSM-treated mice exhibited an enhanced antioxidant status, characterized by increased glutathione peroxidase (GPx) activity and glutathione concentration in plasma, erythrocytes and bone marrow. These improvements were linked to the activation of the nuclear factor E2 related factor 2 (Nrf2) pathway and its downstream antioxidant gene expression, including that of superoxide dismutase and GPx. These findings suggested that age-related bone loss is closely tied to oxidative stress, and MSM supplementation effectively reverses bone loss by mitigating oxidative stress-mediated bone resorption.<\/jats:p>","DOI":"10.3390\/antiox14020216","type":"journal-article","created":{"date-parts":[[2025,2,13]],"date-time":"2025-02-13T12:06:32Z","timestamp":1739448392000},"page":"216","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Protective Effect of Methyl Sulfonyl Methane on the Progression of Age-Induced Bone Loss by Regulating Oxidative Stress-Mediated Bone Resorption"],"prefix":"10.3390","volume":"14","author":[{"given":"Duo","family":"Zhang","sequence":"first","affiliation":[{"name":"Physical Education Department, Civil Aviation Flight University of China, Guanghan 618307, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leilei","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Biochemistry and Nutrition of Agriculture Ministry, College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8797-6318","authenticated-orcid":false,"given":"Lu","family":"Tang","sequence":"additional","affiliation":[{"name":"Physical Education Department, Civil Aviation Flight University of China, Guanghan 618307, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yeting","family":"Zhang","sequence":"additional","affiliation":[{"name":"Physical Education Department, Civil Aviation Flight University of China, Guanghan 618307, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1490-8541","authenticated-orcid":false,"given":"Huaiyong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Biochemistry and Nutrition of Agriculture Ministry, College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China"},{"name":"Laboratory for Animal Nutrition and Animal Product Quality, Department of Animal Sciences and Aquatic Ecology, Ghent University, 9000 Ghent, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Zou","sequence":"additional","affiliation":[{"name":"Physical Education Department, Civil Aviation Flight University of China, Guanghan 618307, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,2,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1016\/j.ecl.2012.04.006","article-title":"Pathophysiology of osteoporosis: New mechanistic insights","volume":"41","author":"Armas","year":"2012","journal-title":"Endocrinol. Metab. Cli. N. Am."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1038\/s41392-022-01251-0","article-title":"Aging and aging-related diseases: From molecular mechanisms to interventions and treatments","volume":"7","author":"Guo","year":"2022","journal-title":"Signal Transduct. Target. Ther."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"757","DOI":"10.2147\/CIA.S158513","article-title":"Oxidative stress, aging, and diseases","volume":"13","author":"Liguori","year":"2018","journal-title":"Clin. Interv. Aging"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"e04107","DOI":"10.1016\/j.heliyon.2020.e04107","article-title":"Aging\u2014Oxidative stress, antioxidants and computational modeling","volume":"6","author":"Warraich","year":"2020","journal-title":"Heliyon"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/j.bbrc.2003.12.073","article-title":"Oxidative stress inhibits osteoblastic differentiation of bone cells by ERK and NF-kappaB","volume":"314","author":"Bai","year":"2004","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/j.cmet.2010.01.001","article-title":"FoxO1 is a positive regulator of bone formation by favoring protein synthesis and resistance to oxidative stress in osteoblasts","volume":"11","author":"Rached","year":"2010","journal-title":"Cell Metab."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2682","DOI":"10.1002\/jbmr.489","article-title":"Cytoplasmic superoxide causes bone fragility owing to low-turnover osteoporosis and impaired collagen cross-linking","volume":"26","author":"Nojiri","year":"2011","journal-title":"J. Bone Miner. Res."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"27285","DOI":"10.1074\/jbc.M702810200","article-title":"Skeletal involution by age-associated oxidative stress and its acceleration by loss of sex steroids","volume":"282","author":"Almeida","year":"2007","journal-title":"J. Biol. Chem."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/S0378-4274(01)00396-4","article-title":"Accumulation of methylsulfonylmethane in the human brain: Identification by multinuclear magnetic resonance spectroscopy","volume":"123","author":"Lin","year":"2001","journal-title":"Toxicol. Lett."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Wong, T., Bloomer, R.J., Benjamin, R.L., and Buddington, R.K. (2017). Small Intestinal Absorption of Methylsulfonylmethane (MSM) and Accumulation of the Sulfur Moiety in Selected Tissues of Mice. Nutrients, 10.","DOI":"10.3390\/nu10010019"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1033","DOI":"10.1021\/jf0621469","article-title":"Pharmacokinetics and distribution of [35S] methylsulfonylmethane following oral administration to rats","volume":"55","author":"Magnuson","year":"2007","journal-title":"J. Agric. Food Chem."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Butawan, M., Benjamin, R.L., and Bloomer, R.J. (2017). Methylsulfonylmethane: Applications and Safety of a Novel Dietary Supplement. Nutrients, 9.","DOI":"10.3390\/nu9030290"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1248\/bpb.32.651","article-title":"The anti-inflammatory effects of methylsulfonylmethane on lipopolysaccharide-induced inflammatory responses in murine macrophages","volume":"32","author":"Kim","year":"2009","journal-title":"Biol. Pharm. Bull."},{"key":"ref_14","first-page":"507278","article-title":"Protective effects of methylsulfonylmethane on hemodynamics and oxidative stress in monocrotaline-induced pulmonary hypertensive rats","volume":"2012","author":"Mohammadi","year":"2012","journal-title":"Adv. Pharmacol. Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"460","DOI":"10.3892\/mmr.2016.5274","article-title":"Methylsulfonylmethane enhances BMP-2-induced osteoblast differentiation in mesenchymal stem cells","volume":"14","author":"Kim","year":"2016","journal-title":"Mol. Med. Rep."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Aljohani, H., Senbanjo, L.T., and Chellaiah, M.A. (2019). Methylsulfonylmethane increases osteogenesis and regulates the mineralization of the matrix by transglutaminase 2 in SHED cells. PLoS ONE, 14.","DOI":"10.1371\/journal.pone.0225598"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"108638","DOI":"10.1016\/j.intimp.2022.108638","article-title":"Methylsulfonylmethane protects against ethanol-induced brain injury in mice through the inhibition of oxidative stress, proinflammatory mediators and apoptotic cell death","volume":"106","author":"Amirshahrokhi","year":"2022","journal-title":"Int. Immunopharmacol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1016\/j.nima.2007.05.099","article-title":"UGCT: New x-ray radiography and tomography facility","volume":"580","author":"Masschaele","year":"2007","journal-title":"Nucl. Instrum. Meth. A."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Zhang, H., Majdeddin, M., Gaublomme, D., Taminiau, B., Boone, M., Elewaut, D., Daube, G., Josipovic, I., Zhang, K., and Michiels, J. (2021). 25-hydroxycholecalciferol reverses heat induced alterations in bone quality in finisher broilers associated with effects on intestinal integrity and inflammation. J. Anim. Sci. Biotechnol., 12.","DOI":"10.1186\/s40104-021-00627-6"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1016\/j.jpba.2006.07.030","article-title":"Rapid quantification of malondialdehyde in plasma by high performance liquid chromatography-visible detection","volume":"43","author":"Grotto","year":"2007","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1016\/S0309-1740(03)00155-4","article-title":"Antioxidant, lipolytic and proteolytic enzyme activities in pork meat from different genotypes","volume":"66","author":"Blasco","year":"2004","journal-title":"Meat Sci."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Degroote, J., Vergauwen, H., Wang, W., Van Ginneken, C., De Smet, S., and Michiels, J. (2020). Changes of the glutathione redox system during the weaning transition in piglets, in relation to small intestinal morphology and barrier function. J. Anim. Sci. Biotechnol., 11.","DOI":"10.1186\/s40104-020-00440-7"},{"key":"ref_23","first-page":"1","article-title":"Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes","volume":"3","author":"Vandesompele","year":"2002","journal-title":"Genome Boil."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"3523","DOI":"10.1007\/s11999-014-3829-x","article-title":"Fractures in geriatric mice show decreased callus expansion and bone volume","volume":"472","author":"Lopas","year":"2014","journal-title":"Clin. Orthop. Relat. Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/j.cmet.2009.12.009","article-title":"FoxO-mediated defense against oxidative stress in osteoblasts is indispensable for skeletal homeostasis in mice","volume":"11","author":"Ambrogini","year":"2010","journal-title":"Cell Metab."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1945","DOI":"10.1002\/jbmr.2709","article-title":"The roles and mechanisms of actions of vitamin C in bone: New developments","volume":"30","author":"Aghajanian","year":"2015","journal-title":"J. Bone Miner Res."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Zhang, H., Pertiwi, H., Michiels, J., Gaublomme, D., Majdeddin, M., Hou, Y., Boone, M., Elewaut, D., Josipovic, I., and Degroote, J. (2024). Improvement of antioxidant capability by dietary N-acetyl cysteine supplementation alleviates bone loss induced by chronic heat stress in finisher broilers. J. Anim. Sci. Biotechnol., 15.","DOI":"10.1186\/s40104-024-01114-4"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"914","DOI":"10.1016\/j.psj.2019.12.010","article-title":"Dietary methylsulfonylmethane supplementation and oxidative stress in broiler chickens","volume":"99","author":"Rasheed","year":"2020","journal-title":"Poult. Sci."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/S8756-3282(02)00830-X","article-title":"Contribution of trabecular and cortical components to the mechanical properties of bone and their regulating parameters","volume":"31","author":"Ito","year":"2002","journal-title":"Bone"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Aljohani, H., Senbanjo, L.T., Al Qranei, M., Stains, J.P., and Chellaiah, M.A. (2021). Methylsulfonylmethane Increases the Alveolar Bone Density of Mandibles in Aging Female Mice. Front. Physiol., 12.","DOI":"10.3389\/fphys.2021.708905"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1007\/s11914-012-0105-4","article-title":"Osteocyte signalling in bone","volume":"10","author":"Schaffler","year":"2012","journal-title":"Curr. Osteoporos. Rep."},{"key":"ref_32","first-page":"2417","article-title":"Consequences of Aging on Bone","volume":"15","author":"Zhang","year":"2023","journal-title":"Aging Dis."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"29496","DOI":"10.3390\/ijms161226179","article-title":"Calycosin Suppresses RANKL-Mediated Osteoclastogenesis through Inhibition of MAPKs and NF-\u03baB","volume":"16","author":"Quan","year":"2015","journal-title":"Int. J. Mol. Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1080\/03008207.2017.1290085","article-title":"Osteoblast-osteoclast interactions","volume":"59","author":"Chen","year":"2018","journal-title":"Connect. Tissue Res."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1038\/16852","article-title":"OPGL is a key regulator of osteoclastogenesis, lymphocyte development and lymph-node organogenesis","volume":"397","author":"Kong","year":"1999","journal-title":"Nature"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1007\/s007740200049","article-title":"Transgenic mice overexpressing soluble osteoclast differentiation factor (sODF) exhibit severe osteoporosis","volume":"20","author":"Mizuno","year":"2002","journal-title":"J. Bone Miner. Metab."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Kim, J.M., Lin, C., Stavre, Z., Greenblatt, M.B., and Shim, J.H. (2020). Osteoblast-osteoclast communication and bone homeostasis. Cells, 9.","DOI":"10.3390\/cells9092073"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Joung, Y.H., Darvin, P., Kang, D.Y., Sp, N., Byun, H.J., Lee, C.H., Lee, H.K., and Yang, Y.M. (2016). Methylsulfonylmethane inhibits RANKL-Induced osteoclastogenesis in BMMs by suppressing NF-\u03baB and STAT3 activities. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0159891"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.freeradbiomed.2018.08.036","article-title":"NADPH oxidases in bone homeostasis and osteoporosis","volume":"132","year":"2019","journal-title":"Free Radic. Biol. Med."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Ohyama, Y., Ito, J., Kitano, V.J., Shimada, J., and Hakeda, Y. (2018). The polymethoxy flavonoid sudachitin suppresses inflammatory bone destruction by directly inhibiting osteoclastogenesis due to reduced ROS production and MAPK activation in osteoclast precursors. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0191192"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"102321","DOI":"10.1016\/j.psj.2022.102321","article-title":"Effect of supplemental methyl sulfonyl methane on performance, carcass and meat quality and oxidative status in chronic cyclic heat-stressed finishing broilers","volume":"102","author":"Zhang","year":"2023","journal-title":"Poult. Sci."},{"key":"ref_42","first-page":"8","article-title":"Antioxidant Systems in Poultry Biology: Superoxide Dismutase","volume":"1","author":"Peter","year":"2016","journal-title":"J. Anim. Res. Nutr."},{"key":"ref_43","first-page":"331","article-title":"Role of glutathione metabolism and glutathione-related antioxidant defense systems in hypertension","volume":"67","author":"Robaczewska","year":"2016","journal-title":"J. Physiol. Pharmacol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1069","DOI":"10.1016\/j.psj.2019.10.002","article-title":"Effects of methylsulfonylmethane on growth performance, immunity, antioxidant capacity, and meat quality in Pekin ducks","volume":"99","author":"Yan","year":"2020","journal-title":"Poult. Sci."},{"key":"ref_45","first-page":"845","article-title":"Effect of single dose administration of methylsulfonylmethane on oxidative stress following acute exhaustive exercise","volume":"12","author":"Niknam","year":"2013","journal-title":"Iran. J. Pharm. Res."},{"key":"ref_46","first-page":"31","article-title":"Methylsulfonylmethane decreases inflammatory response to tumor necrosis factor-\u03b1 in cardiac cells","volume":"8","author":"Miller","year":"2018","journal-title":"Am. J. Cardiovasc. Dis."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"4972","DOI":"10.3382\/ps\/pez265","article-title":"Toxicity and tissue distribution of methylsulfonylmethane following oral gavage in broilers","volume":"98","author":"Oelschlager","year":"2019","journal-title":"Poult. Sci."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Jaganjac, M., Milkovic, L., Sunjic, S.B., and Zarkovic, N. (2020). The NRF2, Thioredoxin, and Glutathione System in Tumorigenesis and Anticancer Therapies. Antioxidants, 9.","DOI":"10.3390\/antiox9111151"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"749","DOI":"10.1089\/152308602760598891","article-title":"Heme oxygenase-1: Redox regulation and role in the hepatic response to oxidative stress","volume":"4","author":"Bauer","year":"2002","journal-title":"Antioxid. Redox Signal."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1016\/j.redox.2013.07.005","article-title":"Potential therapeutic effects of the simultaneous targeting of the Nrf2 and NF-\u03baB pathways in diabetic neuropathy","volume":"1","author":"Negi","year":"2013","journal-title":"Redox Biol."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Egbujor, M.C., Petrosino, M., Zuhra, K., and Saso, L. (2022). The Role of Organosulfur Compounds as Nrf2 Activators and Their Antioxidant Effects. Antioxidants, 11.","DOI":"10.3390\/antiox11071255"},{"key":"ref_52","first-page":"328","article-title":"MSM ameliorates HIV-1 Tat induced neuronal oxidative stress via rebalance of the glutathione cycle","volume":"7","author":"Kim","year":"2015","journal-title":"Am. J. Transl. Res."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1186\/s41232-023-00279-1","article-title":"Bone regeneration in inflammation with aging and cell-based immunomodulatory therapy","volume":"43","author":"Kushioka","year":"2023","journal-title":"Inflamm. Regen."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Wu, Y., Yang, Y., Wang, L., Chen, Y., Han, X., Sun, L., Chen, H., and Chen, Q. (2023). Effect of Bifidobacterium on osteoclasts: TNF-\u03b1\/NF-\u03baB inflammatory signal pathway-mediated mechanism. Front. Endocrinol., 14.","DOI":"10.3389\/fendo.2023.1109296"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1002\/art.11419","article-title":"Systemic tumor necrosis factor alpha mediates an increase in peripheral CD11bhigh osteoclast precursors in tumor necrosis factor alpha-transgenic mice","volume":"50","author":"Li","year":"2004","journal-title":"Arthritis Rheum."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"e5","DOI":"10.1038\/ijos.2017.45","article-title":"Interleukin-1\u03b2 induces human cementoblasts to support osteoclastogenesis","volume":"9","author":"Huynh","year":"2017","journal-title":"Int. J. Oral. Sci."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/j.cyto.2014.11.001","article-title":"Methylsulfonylmethane inhibits NLRP3 inflammasome activation","volume":"71","author":"Ahn","year":"2015","journal-title":"Cytokine"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/j.intimp.2018.10.004","article-title":"Methylsulfonylmethane and mobilee prevent negative effect of IL-1\u03b2 in human chondrocyte cultures via NF-\u03baB signaling pathway","volume":"65","author":"Cheleschi","year":"2018","journal-title":"Int. Immunopharmacol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1226","DOI":"10.1093\/gerona\/gls163","article-title":"Pathogenesis of age-related bone loss in humans","volume":"68","author":"Khosla","year":"2013","journal-title":"J. Gerontol. A Biol. Sci. Med. Sci."}],"container-title":["Antioxidants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-3921\/14\/2\/216\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T16:33:52Z","timestamp":1760027632000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-3921\/14\/2\/216"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2,13]]},"references-count":59,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2025,2]]}},"alternative-id":["antiox14020216"],"URL":"https:\/\/doi.org\/10.3390\/antiox14020216","relation":{},"ISSN":["2076-3921"],"issn-type":[{"value":"2076-3921","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2,13]]}}}