{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T16:13:16Z","timestamp":1790957596612,"version":"4.1.0"},"reference-count":67,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T00:00:00Z","timestamp":1740096000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100010751","name":"FONDECYT Regular projects","doi-asserted-by":"publisher","award":["1220097"],"award-info":[{"award-number":["1220097"]}],"id":[{"id":"10.13039\/501100010751","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010751","name":"FONDECYT Regular projects","doi-asserted-by":"publisher","award":["1230115"],"award-info":[{"award-number":["1230115"]}],"id":[{"id":"10.13039\/501100010751","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010751","name":"FONDECYT Regular projects","doi-asserted-by":"publisher","award":["240263"],"award-info":[{"award-number":["240263"]}],"id":[{"id":"10.13039\/501100010751","id-type":"DOI","asserted-by":"publisher"}]},{"name":"FOVI project","award":["1220097"],"award-info":[{"award-number":["1220097"]}]},{"name":"FOVI project","award":["1230115"],"award-info":[{"award-number":["1230115"]}]},{"name":"FOVI project","award":["240263"],"award-info":[{"award-number":["240263"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>The rising demand for bioactive compounds from marine resources highlights the need for sustainable separation technologies. This study introduces an integrated process combining ultrasound-assisted extraction (USAE) and resin purification (RP) to isolate phlorotannins from Durvillaea incurvata, a brown seaweed with significant biomedical potential. Using a 32.5% ethanol\u2013water solvent system for USAE followed by RP on Diaion HP-20 resin, phlorotannins were enriched 2.4-fold, with simultaneous removal of interfering compounds such as mannitol (~100%), which was demonstrated by FTIR and HPLC-IR analysis. Advanced characterization using UHPLC-QToF-MS\/MS identified five novel phlorotannins with polymerization degrees of 3 to 8 phloroglucinol units in both USAE extracts and post-RP. Mass balance based on spectrophotometric measurements indicated a purification factor of ~2, confirming process effectiveness. RP streams showed distinct phlorotannin profiles, with one phlorotannin exceeding 70% relative abundance. However, MS\/MS results showed significantly lower recoveries than spectrophotometric data, revealing a novel insight into RP purification. These findings highlight the critical role of comprehensive chemical characterization in optimizing sustainable phlorotannin extraction from seaweed. They propose a framework for scalable, eco-efficient technologies for achieving high-purity phlorotannin recovery. This approach facilitates the development of phlorotannin-based applications in the nutraceutical and pharmaceutical industries.<\/jats:p>","DOI":"10.3390\/antiox14030250","type":"journal-article","created":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T09:26:32Z","timestamp":1740129992000},"page":"250","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Sustainable Recovery of Phlorotannins from Durvillaea incurvata: Integrated Extraction and Purification with Advanced Characterization"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8064-3070","authenticated-orcid":false,"given":"Pamela Raquel","family":"Rivera-Tovar","sequence":"first","affiliation":[{"name":"Nutrition and Dietetics School, Faculty of Medicine, Universidad Finis Terrae, Pedro de Valdivia 1509, Providencia, Santiago 7501015, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-7376-4656","authenticated-orcid":false,"given":"Gabriela","family":"Contreras-Contreras","sequence":"additional","affiliation":[{"name":"Nutrition and Dietetics School, Faculty of Medicine, Universidad Finis Terrae, Pedro de Valdivia 1509, Providencia, Santiago 7501015, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulina Isabel","family":"Rivas-Reyes","sequence":"additional","affiliation":[{"name":"Food Technology Section, Department of Analytical Chemistry, Nutrition and Food Science, School of Veterinary Sciences, University of Santiago de Compostela, 27002 Lugo, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2811-4558","authenticated-orcid":false,"given":"Jara","family":"P\u00e9rez-Jim\u00e9nez","sequence":"additional","affiliation":[{"name":"Department of Metabolism and Nutrition, Institute of Food Science, Technology and Nutrition (ICTAN-CSIC), 28040 Madrid, Spain"},{"name":"CIBER de Diabetes y Enfermedades Metab\u00f3licas Asociadas (CIBERDEM), Instituto de Salud Carlos III (ISCIII), 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2959-3164","authenticated-orcid":false,"given":"Maximiliano","family":"Mart\u00ednez-Cifuentes","sequence":"additional","affiliation":[{"name":"Departamento de Qu\u00edmica Org\u00e1nica, Facultad de Ciencias Qu\u00edmicas, Universidad de Concepci\u00f3n, Edmundo Larenas 129, Concepci\u00f3n 4070371, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Ricardo","family":"P\u00e9rez-Correa","sequence":"additional","affiliation":[{"name":"Chemical and Bioprocess Engineering Department, School of Engineering, Pontificia Universidad Cat\u00f3lica de Chile, Vicu\u00f1a Mackenna 4860, Macul, Santiago 7820436, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1357-812X","authenticated-orcid":false,"given":"Mar\u00eda Salom\u00e9","family":"Mariotti-Celis","sequence":"additional","affiliation":[{"name":"Nutrition and Dietetics School, Faculty of Medicine, Universidad Finis Terrae, Pedro de Valdivia 1509, Providencia, Santiago 7501015, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,2,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"400","DOI":"10.1080\/19476337.2017.1411978","article-title":"Green technologies for the extraction of bioactive compounds in fruits and vegetables","volume":"16","author":"Soquetta","year":"2018","journal-title":"CyTA\u2014J. 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