{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T02:55:38Z","timestamp":1791860138626,"version":"4.3.4"},"reference-count":48,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2025,3,6]],"date-time":"2025-03-06T00:00:00Z","timestamp":1741219200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004704","name":"National Research Council of Thailand","doi-asserted-by":"publisher","award":["N42A640325"],"award-info":[{"award-number":["N42A640325"]}],"id":[{"id":"10.13039\/501100004704","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Second Century Fund (C2F), Chulalongkorn University","award":["N42A640325"],"award-info":[{"award-number":["N42A640325"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>Metabolic dysfunction and hepatic abnormalities, such as those associated with high-fat, high-fructose (HFHFr) diets, are major contributors to obesity-related health issues. The growing interest in sustainable dietary interventions has highlighted the potential of plant-based byproducts. Dragon fruit (Hylocereus undatus) peel waste, rich in bioactive compounds such as dietary fibers, phenolics, and betacyanins, represents a promising functional ingredient for managing these disorders. This study investigated the effects of dragon fruit peel powder (DFP) on metabolic dysfunction and hepatic abnormalities induced by a HFHFr diet in rats. Over 12 weeks, the rats were fed a standard AIN-93M diet (control or C), C with 5% (w\/w) DFP (C + DFP), a HFHFr diet, or a HFHFr diet with 5% (w\/w) DFP (HFHFr + DFP). DFP supplementation significantly reduced HFHFr-induced body weight gain, visceral adiposity, insulin resistance, and dyslipidemia while also lowering systolic blood pressure and systemic oxidative stress markers. In the liver, DFP supplementation attenuated fat accumulation and lipid peroxidation, reduced glycogen storage abnormalities, and modulated the expression of lipid metabolism and inflammatory genes. These findings suggest that DFP may serve as a functional dietary supplement for preventing and managing metabolic disorders and liver abnormalities associated with excessive fat and fructose consumption.<\/jats:p>","DOI":"10.3390\/antiox14030319","type":"journal-article","created":{"date-parts":[[2025,3,6]],"date-time":"2025-03-06T06:02:13Z","timestamp":1741240933000},"page":"319","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Dragon Fruit Peel (Hylocereus undatus) Modulates Hepatic Lipid Metabolism and Inflammation in a Rat Model of High-Fat, High-Fructose-Induced Metabolic Dysfunction"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-9326-2423","authenticated-orcid":false,"given":"Siriwan","family":"Chumroenvidhayakul","sequence":"first","affiliation":[{"name":"Center of Excellence in Phytochemical and Functional Food for Clinical Nutrition, Department of Nutrition and Dietetics, Faculty of Allied Health Science, Chulalongkorn University, Bangkok 10330, Thailand"},{"name":"School of Food Industry, King Mongkut\u2019s Institute of Technology Ladkrabang, Bangkok 10520, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thavaree","family":"Thilavech","sequence":"additional","affiliation":[{"name":"Department of Food Chemistry, Faculty of Pharmacy, Mahidol University, Bangkok 10400, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5194-5771","authenticated-orcid":false,"given":"Mahinda Yapa","family":"Abeywardena","sequence":"additional","affiliation":[{"name":"CSIRO Health & Biosecurity, Kintore Avenue, Adelaide, SA 5000, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Conlon","sequence":"additional","affiliation":[{"name":"CSIRO Health & Biosecurity, Kintore Avenue, Adelaide, SA 5000, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie","family":"Dallimore","sequence":"additional","affiliation":[{"name":"CSIRO Health & Biosecurity, Kintore Avenue, Adelaide, SA 5000, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Adams","sequence":"additional","affiliation":[{"name":"CSIRO Health & Biosecurity, Kintore Avenue, Adelaide, SA 5000, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beverly","family":"Muhlhausler","sequence":"additional","affiliation":[{"name":"CSIRO Health & Biosecurity, Kintore Avenue, Adelaide, SA 5000, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7938-6561","authenticated-orcid":false,"given":"Sirichai","family":"Adisakwattana","sequence":"additional","affiliation":[{"name":"Center of Excellence in Phytochemical and Functional Food for Clinical Nutrition, Department of Nutrition and Dietetics, Faculty of Allied Health Science, Chulalongkorn University, Bangkok 10330, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,3,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Alamnia, T.T., Sargent, G.M., and Kelly, M. (2023). Dietary patterns and associations with metabolic risk factors for non-communicable disease. Sci. Rep., 13.","DOI":"10.1038\/s41598-023-47548-0"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Demaria, T.M., Crepaldi, L.D., Costa-Bartuli, E., Branco, J.R., Zancan, P., and Sola-Penna, M. (2023). Once a week consumption of Western diet over twelve weeks promotes sustained insulin resistance and non-alcoholic fat liver disease in C57BL\/6 J mice. Sci. Rep., 13.","DOI":"10.1038\/s41598-023-30254-2"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/j.freeradbiomed.2020.02.025","article-title":"Role of oxidative stress in the pathogenesis of nonalcoholic fatty liver disease","volume":"152","author":"Chen","year":"2020","journal-title":"Free Radic. Biol. Med."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1347","DOI":"10.1042\/CS20220572","article-title":"Molecular mechanisms of metabolic associated fatty liver disease (MAFLD): Functional analysis of lipid metabolism pathways","volume":"136","author":"Badmus","year":"2022","journal-title":"Clin. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"996","DOI":"10.1111\/liv.14773","article-title":"Glycogenosis is common in nonalcoholic fatty liver disease and is independently associated with ballooning, but lower steatosis and lower fibrosis","volume":"41","author":"Allende","year":"2021","journal-title":"Liver Int."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"3533","DOI":"10.1016\/j.clnu.2020.09.001","article-title":"ESPEN practical guideline: Clinical nutrition in liver disease","volume":"39","author":"Bischoff","year":"2020","journal-title":"Clin. Nutr."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"e176","DOI":"10.1038\/ctg.2016.28","article-title":"Short-term hypocaloric high-fiber and high-protein diet improves hepatic steatosis assessed by controlled attenuation parameter","volume":"7","author":"Arslanow","year":"2016","journal-title":"Clin. Transl. Gastroenterol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1111\/liv.12975","article-title":"Natural antioxidants for non-alcoholic fatty liver disease: Molecular targets and clinical perspectives","volume":"36","author":"Salomone","year":"2016","journal-title":"Liver Int."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Wang, R., Yan, R., Jiao, J., Li, F., Zhang, H., Chang, Z., Wei, H., Yan, S., and Li, J. (2024). Fruit and vegetable intake and the risk of non-alcoholic fatty liver disease: A meta-analysis of observational studies. Front. Nutr., 11.","DOI":"10.3389\/fnut.2024.1398184"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1016\/j.tifs.2021.06.040","article-title":"Nutrition, phytochemical profile, bioactivities and applications in food industry of pitaya (Hylocereus spp.) peels: A comprehensive review","volume":"116","author":"Jiang","year":"2021","journal-title":"Trends Food Sci. Technol."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Chumroenvidhayakul, S., Thilavech, T., Abeywardena, M., and Adisakwattana, S. (2022). Investigating the impact of dragon fruit peel waste on starch digestibility, pasting, and thermal properties of flours used in Asia. Foods, 11.","DOI":"10.3390\/foods11142031"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Song, H., Zheng, Z., Wu, J., Lai, J., Chu, Q., and Zheng, X. (2016). White pitaya (Hylocereus undatus) juice attenuates insulin resistance and hepatic steatosis in diet-induced obese mice. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0149670"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1021\/acs.jafc.5b05177","article-title":"Purified betacyanins from Hylocereus undatus peel ameliorate obesity and insulin resistance in high-fat-diet-fed mice","volume":"64","author":"Song","year":"2016","journal-title":"J. Agric. Food Chem."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1462","DOI":"10.1111\/jgh.13278","article-title":"Red pitaya betacyanins protects from diet-induced obesity, liver steatosis and insulin resistance in association with modulation of gut microbiota in mice","volume":"31","author":"Song","year":"2016","journal-title":"J. Gastroenterol. Hepatol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1016\/j.orcp.2023.09.010","article-title":"Rat models of diet-induced obesity and metabolic dysregulation: Current trends, shortcomings and considerations for future research","volume":"17","year":"2023","journal-title":"Obes. Res. Clin. Pract."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1177\/0023677212473717","article-title":"Gender and strain-specific differences in the development of steatosis in rats","volume":"47","author":"Palmes","year":"2013","journal-title":"Lab. Anim."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1124\/jpet.116.237586","article-title":"Effects of antihypertensive agents on intestinal contractility in the spontaneously hypertensive rat: Angiotensin receptor system downregulation by losartan","volume":"360","author":"Patten","year":"2017","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"501","DOI":"10.6026\/97320630018501","article-title":"Effect of RFEMR on NSE and MDA levels in Sprague Dawley rats","volume":"18","author":"Pagadala","year":"2022","journal-title":"Bioinformation"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1007\/s12291-009-0075-1","article-title":"Attenuation of oxidative stress in streptozotocin-induced diabetic rats by Eucalyptus globulus","volume":"24","author":"Nakhaee","year":"2009","journal-title":"Indian J. Clin. Biochem."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1139\/y59-099","article-title":"A rapid method of total lipid extraction and purification","volume":"37","author":"Bligh","year":"1959","journal-title":"Can. J. Biochem. Physiol."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Mamikutty, N., Thent, Z.C., Sapri, S.R., Sahruddin, N.N., Mohd Yusof, M.R., and Haji Suhaimi, F. (2014). The establishment of metabolic syndrome model by induction of fructose drinking water in male Wistar rats. BioMed Res. Int., 2014.","DOI":"10.1155\/2014\/263897"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Chan, A.M.L., Ng, A.M.H., Mohd Yunus, M.H., Idrus, R.B.H., Law, J.X., Yazid, M.D., Chin, K.Y., Shamsuddin, S.A., and Lokanathan, Y. (2021). Recent developments in rodent models of high-fructose diet-induced metabolic syndrome: A systematic review. Nutrients, 13.","DOI":"10.3390\/nu13082497"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2669","DOI":"10.1007\/s00394-018-1814-y","article-title":"The amounts and contributions of total drinking fluids and water from food to total water intake of young adults in Baoding, China","volume":"58","author":"Zhang","year":"2019","journal-title":"Eur. J. Nutr."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"359","DOI":"10.2147\/DMSO.S338113","article-title":"The association between fecal short-chain fatty acids, gut microbiota, and visceral fat in monozygotic twin pairs","volume":"15","author":"Yin","year":"2022","journal-title":"Diabetes Metab. Syndr. Obes."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"120924","DOI":"10.1016\/j.lfs.2022.120924","article-title":"Oxidative and endoplasmic reticulum stress develop adverse metabolic effects due to the high-fat high-fructose diet consumption from birth to young adulthood","volume":"309","author":"Izadi","year":"2022","journal-title":"Life Sci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1016\/j.pcad.2020.04.009","article-title":"Small dense low-density lipoprotein-cholesterol (sdLDL-C): Analysis, effects on cardiovascular endpoints and dietary strategies","volume":"63","author":"Santos","year":"2020","journal-title":"Prog. Cardiovasc. Dis."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"012167","DOI":"10.1088\/1742-6596\/1013\/1\/012167","article-title":"The role of red dragon fruit peel (Hylocereus polyrhizus) to improvement blood lipid levels of hyperlipidaemia male mice","volume":"1013","author":"Hernawati","year":"2018","journal-title":"J. Phys. Conf. Ser."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Campos-Perez, W., and Martinez-Lopez, E. (2021). Effects of short chain fatty acids on metabolic and inflammatory processes in human health. Biochim. Biophys. Acta Mol. Cell Biol. Lipids, 1866.","DOI":"10.1016\/j.bbalip.2021.158900"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"6807","DOI":"10.1039\/D0FO00537A","article-title":"The effects of betalain-rich cacti (dragon fruit and cactus pear) on endothelial and vascular function: A systematic review of animal and human studies","volume":"11","author":"Cheok","year":"2020","journal-title":"Food Funct."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1139\/y06-080","article-title":"Fructose-induced hypertension in Wistar-Kyoto rats: Interaction with moderately high dietary salt","volume":"85","author":"Vasdev","year":"2007","journal-title":"Can. J. Physiol. Pharmacol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"912","DOI":"10.1097\/HJH.0000000000000551","article-title":"The mechanisms underlying fructose-induced hypertension: A review","volume":"33","author":"Klein","year":"2015","journal-title":"J. Hypertens."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1186\/s12986-019-0421-0","article-title":"Functional properties of beetroot (Beta vulgaris) in management of cardio-metabolic diseases","volume":"17","author":"Mirmiran","year":"2020","journal-title":"Nutr. Metab."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"126169","DOI":"10.1016\/j.foodchem.2020.126169","article-title":"Characterization of the profile and concentration of betacyanin in the gastric content, blood and urine of rats after an intragastric administration of fermented red beet juice","volume":"313","author":"Sawicki","year":"2020","journal-title":"Food Chem."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.nfs.2020.08.001","article-title":"Whole beetroot consumption reduces systolic blood pressure and modulates diversity and composition of the gut microbiota in older participants","volume":"21","author":"Capper","year":"2020","journal-title":"NFS J."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"8286","DOI":"10.1039\/C9FO02020A","article-title":"Effects of betalains on atherogenic risk factors in patients with atherosclerotic cardiovascular disease","volume":"10","author":"Rahimi","year":"2019","journal-title":"Food Funct."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Hu, W., Cassard, A.M., and Ciocan, D. (2022). Pectin in metabolic liver disease. Nutrients, 15.","DOI":"10.3390\/nu15010157"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1186\/s12944-019-1024-5","article-title":"The severity of rat liver injury by fructose and high fat depends on the degree of respiratory dysfunction and oxidative stress induced in mitochondria","volume":"18","year":"2019","journal-title":"Lipids Health Dis."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1016\/j.jff.2013.10.003","article-title":"In vivo and in vitro antioxidant effects of beetroot pomace extracts","volume":"6","author":"Djilas","year":"2014","journal-title":"J. Funct. Foods"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"DiNunzio, G., Belew, G.D., Torres, A.N., Silva, J.G., Silva, L.P., Barosa, C., Tavares, L., and Jones, J.G. (2020). Determining the contribution of a high-fructose corn syrup formulation to hepatic glycogen synthesis during ad-libitum feeding in mice. Sci. Rep., 10.","DOI":"10.1038\/s41598-020-69820-3"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1186\/s12986-016-0074-1","article-title":"High-fructose and high-fat diet-induced disorders in rats: Impact on diabetes risk, hepatic and vascular complications","volume":"13","author":"Lozano","year":"2016","journal-title":"Nutr. Metab."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1210\/en.2012-1725","article-title":"The role of the carbohydrate response element-binding protein in male fructose-fed rats","volume":"154","author":"Erion","year":"2013","journal-title":"Endocrinology"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1765","DOI":"10.1016\/j.bbadis.2015.05.015","article-title":"Recent insights on the role of cholesterol in non-alcoholic fatty liver disease","volume":"1852","author":"Arguello","year":"2015","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Chyau, C.C., Wang, H.F., Zhang, W.J., Chen, C.C., Huang, S.H., Chang, C.C., and Peng, R.Y. (2020). Antrodan alleviates high-fat and high-fructose diet-induced fatty liver disease in C57BL\/6 mice model via AMPK\/Sirt1\/SREBP-1c\/PPAR\u03b3 pathway. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21010360"},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Dziadek, K., Kope\u0107, A., Pi\u0105tkowska, E., and Leszczy\u0144ska, T. (2019). High-fructose diet-induced metabolic disorders were counteracted by the intake of fruit and leaves of sweet cherry in Wistar rats. Nutrients, 11.","DOI":"10.3390\/nu11112638"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Hu, M., Zhang, L., Ruan, Z., Han, P., and Yu, Y. (2021). The regulatory effects of citrus peel powder on liver metabolites and gut flora in mice with non-alcoholic fatty liver disease (NAFLD). Foods, 10.","DOI":"10.3390\/foods10123022"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"2375","DOI":"10.1007\/s00217-024-04545-5","article-title":"Pitahaya (Hylocereus ocamponis) peel flour as new ingredient in the development of beef burgers: Impact on the quality parameters","volume":"250","year":"2024","journal-title":"Eur. Food Res. Technol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"e14491","DOI":"10.1111\/jfpp.14491","article-title":"Effect of pitaya peel powder addition on the phytochemical and textural properties and sensory acceptability of dried and cooked noodles","volume":"44","author":"Shiau","year":"2020","journal-title":"J. Food Process. Preserv."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Chumroenvidhayakul, S., Thilavech, T., Abeywardena, M., and Adisakwattana, S. (2023). Dragon fruit peel waste (Hylocereus undatus) as a potential ingredient for reducing lipid peroxidation, dietary advanced glycation end products, and starch digestibility in cookies. Antioxidants, 12.","DOI":"10.3390\/antiox12051002"}],"container-title":["Antioxidants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-3921\/14\/3\/319\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T16:48:09Z","timestamp":1760028489000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-3921\/14\/3\/319"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,6]]},"references-count":48,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2025,3]]}},"alternative-id":["antiox14030319"],"URL":"https:\/\/doi.org\/10.3390\/antiox14030319","relation":{},"ISSN":["2076-3921"],"issn-type":[{"value":"2076-3921","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,6]]}}}