{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T12:15:20Z","timestamp":1790165720678,"version":"4.1.0"},"reference-count":67,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2025,3,29]],"date-time":"2025-03-29T00:00:00Z","timestamp":1743206400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Science and Technology Council, Taiwan","award":["NSTC 112-2320-B-A49-028-MY3"],"award-info":[{"award-number":["NSTC 112-2320-B-A49-028-MY3"]}]},{"name":"National Science and Technology Council, Taiwan","award":["MOST 109-2320-B-010-029-MY3"],"award-info":[{"award-number":["MOST 109-2320-B-010-029-MY3"]}]},{"name":"National Science and Technology Council, Taiwan","award":["CGMH-NYCU-113-CORPG8P0271"],"award-info":[{"award-number":["CGMH-NYCU-113-CORPG8P0271"]}]},{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology, Taiwan","doi-asserted-by":"publisher","award":["NSTC 112-2320-B-A49-028-MY3"],"award-info":[{"award-number":["NSTC 112-2320-B-A49-028-MY3"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology, Taiwan","doi-asserted-by":"publisher","award":["MOST 109-2320-B-010-029-MY3"],"award-info":[{"award-number":["MOST 109-2320-B-010-029-MY3"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology, Taiwan","doi-asserted-by":"publisher","award":["CGMH-NYCU-113-CORPG8P0271"],"award-info":[{"award-number":["CGMH-NYCU-113-CORPG8P0271"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Kaohsiung Chang Gung Memorial Hospital and National Yang Ming Chiao Tung University Joint Research Program","award":["NSTC 112-2320-B-A49-028-MY3"],"award-info":[{"award-number":["NSTC 112-2320-B-A49-028-MY3"]}]},{"name":"Kaohsiung Chang Gung Memorial Hospital and National Yang Ming Chiao Tung University Joint Research Program","award":["MOST 109-2320-B-010-029-MY3"],"award-info":[{"award-number":["MOST 109-2320-B-010-029-MY3"]}]},{"name":"Kaohsiung Chang Gung Memorial Hospital and National Yang Ming Chiao Tung University Joint Research Program","award":["CGMH-NYCU-113-CORPG8P0271"],"award-info":[{"award-number":["CGMH-NYCU-113-CORPG8P0271"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>Leaves of Eriobotrya japonica have long been utilized in traditional Chinese medicine (TCM) for treating pulmonary inflammation and stomach disorders. This study extends their pharmacological applications by evaluating the antioxidant, anti-\u03b1-glucosidase, anti-acetylcholinesterase (AChE), and anti-inflammatory activities of solvent extracts and isolated bioactive components through an integrative approach combining extraction, bioassays, and molecular docking. Solvent extracts prepared with varying polarities exhibited distinct bioactivities, with the 100 \u00b0C water and methanol extracts displaying the strongest antioxidant potential. The ethyl acetate extract exhibited potent \u03b1-glucosidase inhibition, whereas the n-hexane extract demonstrated significant AChE inhibitory activity. Among the isolated compounds, epicatechin (5) (SC50 = 7.83 \u00b1 0.34 \u03bcM) and rutin (6) (SC50 = 6.69 \u00b1 0.25 \u03bcM) showed superior ABTS and superoxide scavenging activities, respectively, compared to the positive controls (BHT and cynaroside). Ursolic acid (2) exhibited stronger \u03b1-glucosidase inhibition (IC50 = 10.68 \u00b1 0.76 \u03bcM) than acarbose (IC50 = 419.93 \u00b1 29.15 \u03bcM), while tormentic acid (4) demonstrated superior AChE inhibition compared to chlorogenic acid. Ursolic acid (2) also displayed NO inhibition (IC50 = 20.18 \u00b1 1.46 \u03bcM) comparable to quercetin (IC50 = 17.05 \u00b1 1.63 \u03bcM), with Western blot analysis confirming its potent iNOS inhibitory activity. Molecular docking further supported these findings, revealing that ursolic acid (2) exhibited stronger binding affinity to \u03b1-glucosidase (\u22128.7 kcal\/mol) than acarbose (\u22125.1 kcal\/mol), tormentic acid (4) displayed higher binding energy to AChE (\u22128.8 kcal\/mol) compared to chlorogenic acid (\u22127.8 kcal\/mol), and ursolic acid (2) (\u22127.5 kcal\/mol) showed a binding affinity to iNOS similar to that of quercetin (\u22127.7 kcal\/mol). These results highlight the strong potential of E. japonica leaf extracts and bioactive compounds as natural antioxidants, enzyme inhibitors, and anti-inflammatory agents, supporting their development as dietary supplements or therapeutic candidates for managing oxidative stress, hyperglycemia, neurodegenerative diseases, and inflammatory disorders.<\/jats:p>","DOI":"10.3390\/antiox14040413","type":"journal-article","created":{"date-parts":[[2025,3,31]],"date-time":"2025-03-31T05:10:07Z","timestamp":1743397807000},"page":"413","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Pharmacological and Molecular Docking Investigation of Leaves of Eriobotrya japonica: Antioxidant, Enzyme Inhibition, and Anti-Inflammatory Effects"],"prefix":"10.3390","volume":"14","author":[{"given":"Pao-Jen","family":"Kuo","sequence":"first","affiliation":[{"name":"Department of Plastic Surgery, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung 833401, Taiwan"},{"name":"College of Medicine, Chang Gung University, Taoyuan 333323, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li-Ting","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, School of Pharmaceutical Sciences, National Yang Ming Chiao Tung University, Taipei 112304, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-8662-8816","authenticated-orcid":false,"given":"Sin-Min","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, School of Pharmaceutical Sciences, National Yang Ming Chiao Tung University, Taipei 112304, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zih-Rong","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, School of Pharmaceutical Sciences, National Yang Ming Chiao Tung University, Taipei 112304, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5680-9533","authenticated-orcid":false,"given":"Jih-Jung","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, School of Pharmaceutical Sciences, National Yang Ming Chiao Tung University, Taipei 112304, Taiwan"},{"name":"Department of Medical Research, China Medical University Hospital, China Medical University, Taichung 404333, Taiwan"},{"name":"Traditional Herbal Medicine Research Center, Taipei Medical University Hospital, Taipei 110301, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,3,29]]},"reference":[{"key":"ref_1","first-page":"1","article-title":"The role of reactive oxygen species and antioxidants in oxidative stress","volume":"1","author":"Ozougwu","year":"2016","journal-title":"Int. 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