{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T23:08:15Z","timestamp":1790636895620,"version":"4.1.0"},"reference-count":41,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2025,4,18]],"date-time":"2025-04-18T00:00:00Z","timestamp":1744934400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"European Union\u2014Next-GenerationEU","award":["BG-RRP-2.004-0001-C01"],"award-info":[{"award-number":["BG-RRP-2.004-0001-C01"]}]},{"name":"National Recovery and Resilience Plan of the Republic of Bulgaria","award":["BG-RRP-2.004-0001-C01"],"award-info":[{"award-number":["BG-RRP-2.004-0001-C01"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>Antioxidants from natural sources are essential for the development of new therapeutics to improve human health. The objects of study are the aerial flowering parts of Potentilla argentea, a plant species known in traditional medicine for the astringent, hemostatic, wound-healing, and anti-inflammatory effects of its rhizomes. A Potentillae argenteae herba ethanol dry tincture was chromatographically analyzed (GC\/MS, HPLC) and its antioxidant (ABTS, DPPH, CUPRAC, FRAP assays) and DNA nicking protection potentials were evaluated. The eighteen volatiles were identified by GC\/MS, where the predominant components were n-nonacosane (39.38 mg\/g dt), squalene (28.88 mg\/g dt), n-tricosane (18.36 mg\/g dt), ethyl oleate (15.24 mg\/g dt), and n-pentacosane (10.60 mg\/g dt). A high content of total polyphenols was obtained (440.78 mg GAE\/g dt), and HPLC analysis identified two flavonoids and three phenolic acids, of which rosmarinic acid and rutin were above 10 mg\/g dt. The tincture exhibited strong antioxidant activity by all four methods, especially CUPRAC assay (8617.54 \u03bcM TE\/g). DNA protective activity against oxidative damage and microscopic identification of P. argenteae herba powder were established for the first time. Therefore, the tincture could be incorporated into phytopreparations for the treatment of human diseases caused by reactive oxygen species.<\/jats:p>","DOI":"10.3390\/antiox14040487","type":"journal-article","created":{"date-parts":[[2025,4,18]],"date-time":"2025-04-18T03:27:46Z","timestamp":1744946866000},"page":"487","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Potentillae argenteae herba\u2014Antioxidant and DNA-Protective Activities, and Microscopic Characters"],"prefix":"10.3390","volume":"14","author":[{"given":"Tsvetelina","family":"Andonova","sequence":"first","affiliation":[{"name":"Department of Botany and Biological Education, Faculty of Biology, University of Plovdiv \u201cPaisii Hilendarski\u201d, 4000 Plovdiv, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9253-2001","authenticated-orcid":false,"given":"Yordan","family":"Muhovski","sequence":"additional","affiliation":[{"name":"Biological Engineering Unit, Life Sciences Department, Walloon Agricultural Research Centre, 5030 Gembloux, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samir","family":"Naimov","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Faculty of Biology, University of Plovdiv \u201cPaisii Hilendarski\u201d, 4000 Plovdiv, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1094-8009","authenticated-orcid":false,"given":"Elena","family":"Apostolova","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Faculty of Biology, University of Plovdiv \u201cPaisii Hilendarski\u201d, 4000 Plovdiv, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4570-0716","authenticated-orcid":false,"given":"Silviya","family":"Mladenova","sequence":"additional","affiliation":[{"name":"Department of Human Anatomy and Physiology, Faculty of Biology, University of Plovdiv \u201cPaisii Hilendarski\u201d, 4000 Plovdiv, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0276-4170","authenticated-orcid":false,"given":"Ivayla","family":"Dincheva","sequence":"additional","affiliation":[{"name":"Department of Agrobiotechnologies, AgroBioInstitute, Agricultural Academy, 1164 Sofia, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9234-0779","authenticated-orcid":false,"given":"Vasil","family":"Georgiev","sequence":"additional","affiliation":[{"name":"Laboratory of Cell Biosystems, Institute of Microbiology, Bulgarian Academy of Sciences, 139 Ruski Blvd., 4000 Plovdiv, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1559-7343","authenticated-orcid":false,"given":"Atanas","family":"Pavlov","sequence":"additional","affiliation":[{"name":"Laboratory of Cell Biosystems, Institute of Microbiology, Bulgarian Academy of Sciences, 139 Ruski Blvd., 4000 Plovdiv, Bulgaria"},{"name":"Department of Analytical Chemistry and Physical Chemistry, Technological Faculty, University of Food Technologies, 4002 Plovdiv, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rumen","family":"Mladenov","sequence":"additional","affiliation":[{"name":"Department of Botany and Biological Education, Faculty of Biology, University of Plovdiv \u201cPaisii Hilendarski\u201d, 4000 Plovdiv, Bulgaria"},{"name":"Department of Medical Biochemistry, Faculty of Pharmacy, Medical University of Plovdiv, 15A Vasil Aprilov Blvd., 4002 Plovdiv, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0193-5014","authenticated-orcid":false,"given":"Ivanka","family":"Dimitrova-Dyulgerova","sequence":"additional","affiliation":[{"name":"Department of Botany and Biological Education, Faculty of Biology, University of Plovdiv \u201cPaisii Hilendarski\u201d, 4000 Plovdiv, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,4,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"100460","DOI":"10.1016\/j.hermed.2021.100460","article-title":"A Holistic Overview of Different Species of Potentilla a Medicinally Important Plant along with Their Pharmaceutical Significance: A Review","volume":"29","author":"Kumari","year":"2021","journal-title":"J. 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