{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T23:45:52Z","timestamp":1785368752854,"version":"3.55.0"},"reference-count":85,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2025,5,16]],"date-time":"2025-05-16T00:00:00Z","timestamp":1747353600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Foundation","award":["81573143"],"award-info":[{"award-number":["81573143"]}]},{"name":"National Natural Science Foundation","award":["81872612"],"award-info":[{"award-number":["81872612"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>The protective effect of cruciferae-derived sulforaphane (SFN) on diabetic cardiomyopathy (DCM) has garnered increasing attention. However, no studies have specifically explored its mechanistic involvement in cardiac substrate metabolism and mitochondrial function. To address this gap, Type 2 diabetes mellitus (T2DM) db\/db mice were orally gavaged with vehicle or 10 mg\/kg body weight SFN every other day for 16 weeks, with vehicle-treated wild-type mice as controls. SFN intervention (SFN-I) alleviated hyperglycemia, dyslipidemia, HOMA-IR, serum MDA levels, and liver inflammation. Furthermore, SFN-I improved the lipotoxicity-related phenotype of T2DM cardiomyopathy, manifested as attenuation of diastolic dysfunction, cardiac injury, fibrosis, lipid accumulation and peroxidation, ROS generation, and decreased mitochondrial complex I and II activities and ATP content, despite having no effect on ceramide abnormalities. Protein expression data revealed that the model mice exhibited upregulated cardiac CD36, H-FABP, FATP4, CPT1B, PPAR\u03b1, and PDK4 but downregulated GLUT4, with unchanged MPC1 and MPC2. Notably, SFN-I significantly attenuated the increase in CD36, H-FABP, CPT1B, and PPAR\u03b1. These results suggest that chronic oral SFN-I protects against DCM by mitigating overall metabolic dysregulation and inhibiting cardiolipotoxicity. The latter might involve controlling cardiac fatty acid metabolism and improving mitochondrial function, rather than promoting glucose metabolism.<\/jats:p>","DOI":"10.3390\/antiox14050603","type":"journal-article","created":{"date-parts":[[2025,5,20]],"date-time":"2025-05-20T04:48:18Z","timestamp":1747716498000},"page":"603","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Oral Sulforaphane Intervention Protects Against Diabetic Cardiomyopathy in db\/db Mice: Focus on Cardiac Lipotoxicity and Substrate Metabolism"],"prefix":"10.3390","volume":"14","author":[{"given":"Pan","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Nutrition, School of Public Health, Sun Yat-Sen University, Guangzhou 510080, China"},{"name":"Guangdong Provincial Key Laboratory of Food, Nutrition and Health, Guangzhou 510080, China"},{"name":"Guangdong Engineering Technology Center of Nutrition Transformation, Guangzhou 510080, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziling","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Nutrition, School of Public Health, Sun Yat-Sen University, Guangzhou 510080, China"},{"name":"Guangdong Provincial Key Laboratory of Food, Nutrition and Health, Guangzhou 510080, China"},{"name":"Guangdong Engineering Technology Center of Nutrition Transformation, Guangzhou 510080, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyuan","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Nutrition, School of Public Health, Sun Yat-Sen University, Guangzhou 510080, China"},{"name":"Guangdong Provincial Key Laboratory of Food, Nutrition and Health, Guangzhou 510080, China"},{"name":"Guangdong Engineering Technology Center of Nutrition Transformation, Guangzhou 510080, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengdi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Nutrition, School of Public Health, Sun Yat-Sen University, Guangzhou 510080, China"},{"name":"Guangdong Provincial Key Laboratory of Food, Nutrition and Health, Guangzhou 510080, China"},{"name":"Guangdong Engineering Technology Center of Nutrition Transformation, Guangzhou 510080, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengfan","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Nutrition, School of Public Health, Sun Yat-Sen University, Guangzhou 510080, China"},{"name":"Guangdong Provincial Key Laboratory of Food, Nutrition and Health, Guangzhou 510080, China"},{"name":"Guangdong Engineering Technology Center of Nutrition Transformation, Guangzhou 510080, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6458-6096","authenticated-orcid":false,"given":"Dan","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Nutrition, School of Public Health, Sun Yat-Sen University, Guangzhou 510080, China"},{"name":"Guangdong Provincial Key Laboratory of Food, Nutrition and Health, Guangzhou 510080, China"},{"name":"Guangdong Engineering Technology Center of Nutrition Transformation, Guangzhou 510080, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,5,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1501","DOI":"10.1161\/CIRCRESAHA.120.315913","article-title":"Basic Mechanisms of Diabetic Heart Disease","volume":"126","author":"Ritchie","year":"2020","journal-title":"Circ. 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