{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T05:30:23Z","timestamp":1790314223696,"version":"4.1.0"},"reference-count":46,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2025,5,23]],"date-time":"2025-05-23T00:00:00Z","timestamp":1747958400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>Basil (Ocimum basilicum L.) has been traditionally used in various cultures for its medicinal properties. This study evaluated the antioxidant and antimicrobial activities of basil essential oil (BEO) and identified its key bioactive compounds. Antioxidant activity testing, as determined by DPPH and ABTS assays, returned EC50 values of 115.36 and 54.77 \u00b5g\/mL, respectively. BEO demonstrated significant antimicrobial effects against Gardnerella vaginalis, Fannyhessea vaginae, Chryseobacterium gleum, and Candida albicans, with inhibition zones of up to 25.88 mm and MIC values ranging from 31 to 500 \u00b5g\/mL. GC-MS (gas chromatography\u2013mass spectrometry) identified monoterpene, phenylpropene, and sesquiterpene derivatives in BEO. In addition, Fraction 3 (Fr. 3) obtained by preparative HPLC had the highest antimicrobial activity, and methyl trans-cinnamate was identified as the primary active compound in this fraction. BEO had no toxic effect on Lactobacillus crispatus or human dermal fibroblasts. These findings support the traditional use of basil and highlight its potential as a safe, natural therapeutic agent with antioxidant and antimicrobial properties.<\/jats:p>","DOI":"10.3390\/antiox14060628","type":"journal-article","created":{"date-parts":[[2025,5,23]],"date-time":"2025-05-23T12:24:18Z","timestamp":1748003058000},"page":"628","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Antimicrobial Evaluation and Fraction-Based Profiling of Basil Essential Oil Against Vaginal Pathogens"],"prefix":"10.3390","volume":"14","author":[{"given":"Minkyoung","family":"Park","sequence":"first","affiliation":[{"name":"Department of Food Science and Nutrition, College of Human Ecology, Pusan National University, Busan 46241, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9561-4667","authenticated-orcid":false,"given":"Jumin","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Food Science and Nutrition, College of Human Ecology, Pusan National University, Busan 46241, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5144-1725","authenticated-orcid":false,"given":"Dae Youn","family":"Hwang","sequence":"additional","affiliation":[{"name":"Department of Biomaterials Science, College of Natural Resources and Life Science, Pusan National University, Miryang 50463, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sohae","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Biomaterials Science, College of Natural Resources and Life Science, Pusan National University, Miryang 50463, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heeseob","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Food Science and Nutrition, College of Human Ecology, Pusan National University, Busan 46241, Republic of Korea"},{"name":"Longevity & Wellbeing Research Center, Pusan National University, Miryang 50463, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,5,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Parham, S., Kharazi, A.Z., Bakhsheshi-Rad, H.R., Nur, H., Ismail, A.F., Sharif, S., RamaKrishna, S., and Berto, F. 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