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This study investigates the effects of antioxidant lipids (AL\u03c9-3), synthetized through enzymatic acidolysis using non-specific lipase B from Candida antarctica under supercritical CO2 conditions. These lipids were derived from a concentrate of rainbow trout (Oncorhynchus mykiss) belly oil, rich in long-chain polyunsaturated omega-3 fatty acids (LCPUFAn-3), and cold-pressed maqui seed oil (MO, Aristotelia chilensis (Mol.) Stuntz). Their effects were then evaluated in a murine high-fat diet (HFD) model. The fatty acid profile, tocopherol and tocotrienol content, and thin-layer chromatography of AL\u03c9-3 were analyzed. After 8 weeks on an HFD, male C57BL\/6 mice were divided into four groups and switched to a control diet (CD) with the following supplements for 3 weeks: Glycerol (G), commercial marine Omega-3 (CM\u03c9-3), a mixture of LCPUFAn-3 concentrate + MO (M\u03c9-3), or AL\u03c9-3. The total body and organ weights, serum markers, and liver and visceral fat pro-inflammatory marker expression levels were assessed. AL\u03c9-3 contained 13.4% oleic, 33.9% linoleic, 6.3% \u03b1-linolenic, 10.7% eicosapentaenoic, and 16.2% docosahexaenoic fatty acids. The \u03b2, \u03b3, \u03b4-tocopherol, and \u03b2, \u03b3-tocotrienol values were 22.9 \u00b1 1.4, 24.9 \u00b1 0.2, 6.8 \u00b1 0.7, 22.9 \u00b1 1.7, and 22.4 \u00b1 4.7 mg\u00b7kg\u22121, respectively, with \u03b1-tocopherol detected in traces. AL\u03c9-3 supplementation increased serum Trolox equivalent capacity, significantly reduced serum GPT levels (p &lt; 0.01), and enhanced postprandial glucose tolerance (p &lt; 0.001), although it did not alter insulin resistance (HOMA-IR). These findings indicate AL\u03c9-3\u2032s potential for mitigating the glucose intolerance, liver damage, and oxidative stress associated with obesity and MetS, highlighting the need for additional research to explore its potential health benefits.<\/jats:p>","DOI":"10.3390\/antiox14070790","type":"journal-article","created":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T10:45:08Z","timestamp":1751366708000},"page":"790","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Effect of Supplementation of Antioxidant Lipids Synthetized by Enzymatic Acidolysis with EPA\/DHA Concentrate and Maqui (Aristotelia chilensis (Mol.) Stuntz) Seed Oil for Mitigating High-Fat Diet-Induced Obesity and Metabolic Disorders in Mice"],"prefix":"10.3390","volume":"14","author":[{"given":"Benjam\u00edn","family":"Claria","sequence":"first","affiliation":[{"name":"Department of Food Science and Chemical Technology, Faculty of Chemical and Pharmaceutical Sciences, University of Chile, Dr. Carlos Lorca Tobar 964, Santiago 8380494, Chile"},{"name":"Department of Medical Technology, Faculty of Medicine, University of Chile, Santiago 8380000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4779-5341","authenticated-orcid":false,"given":"Alejandra","family":"Espinosa","sequence":"additional","affiliation":[{"name":"Department of Medical Technology, Faculty of Medicine, University of Chile, Santiago 8380000, Chile"},{"name":"School of Medicine, Faculty of Medicine, Universidad de Valpara\u00edso, San Felipe 2172972, Chile"},{"name":"Center of Interdisciplinary Biomedical and Engineering Research for Health, Universidad de Valpara\u00edso, San Felipe 2172972, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2750-2468","authenticated-orcid":false,"given":"Alicia","family":"Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"Department of Food Science and Chemical Technology, Faculty of Chemical and Pharmaceutical Sciences, University of Chile, Dr. Carlos Lorca Tobar 964, Santiago 8380494, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4548-6118","authenticated-orcid":false,"given":"Mar\u00eda Elsa","family":"Pando","sequence":"additional","affiliation":[{"name":"Department of Nutrition, Faculty of Medicine, University of Chile, Santiago 8380000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0334-3661","authenticated-orcid":false,"given":"Gretel","family":"Dovale-Rosabal","sequence":"additional","affiliation":[{"name":"Department of Food Science and Chemical Technology, Faculty of Chemical and Pharmaceutical Sciences, University of Chile, Dr. Carlos Lorca Tobar 964, Santiago 8380494, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2342-5928","authenticated-orcid":false,"given":"Nalda","family":"Romero","sequence":"additional","affiliation":[{"name":"Department of Food Science and Chemical Technology, Faculty of Chemical and Pharmaceutical Sciences, University of Chile, Dr. Carlos Lorca Tobar 964, Santiago 8380494, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katherynne","family":"Mayorga","sequence":"additional","affiliation":[{"name":"Department of Food Science and Chemical Technology, Faculty 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A Consensus Statement from the International Diabetes Federation","volume":"23","author":"Alberti","year":"2006","journal-title":"Diabet. Med."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Hayden, M.R. (2023). Overview and New Insights into the Metabolic Syndrome: Risk Factors and Emerging Variables in the Development of Type 2 Diabetes and Cerebrocardiovascular Disease. Medicina, 59.","DOI":"10.20944\/preprints202302.0115.v1"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"284","DOI":"10.7861\/clinmed.2023-0168","article-title":"Causes of Obesity: A Review","volume":"23","author":"Masood","year":"2023","journal-title":"Clin. Med."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"600","DOI":"10.1038\/s42255-024-00985-1","article-title":"From NAFLD to MASLD: Implications of the New Nomenclature for Preclinical and Clinical Research","volume":"6","author":"Hsu","year":"2024","journal-title":"Nat. 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Antioxidants, 13.","DOI":"10.3390\/antiox13070845"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"937","DOI":"10.1007\/s00217-017-3013-0","article-title":"Maximization of the Docosahexaenoic and Eicosapentaenoic Acids Content in Concentrates Obtained from a By-Product of Rainbow Trout (Oncorhynchus mykiss) Processing","volume":"244","author":"Pando","year":"2018","journal-title":"Eur. Food Res. Technol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"137910","DOI":"10.1016\/j.foodchem.2023.137910","article-title":"Delivery Systems Designed to Enhance Stability and Suitability of Lipophilic Bioactive Compounds in Food Processing: A Review","volume":"437","author":"Lee","year":"2024","journal-title":"Food Chem."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/j.freeradbiomed.2014.03.035","article-title":"Natural Forms of Vitamin E: Metabolism, Antioxidant, and Anti-Inflammatory Activities and Their Role in Disease Prevention and Therapy","volume":"72","author":"Jiang","year":"2014","journal-title":"Free Radic. Biol. Med."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1016\/j.pharmthera.2015.12.003","article-title":"Vitamin E Therapy beyond Cancer: Tocopherol versus Tocotrienol","volume":"162","author":"Peh","year":"2016","journal-title":"Pharmacol. Ther."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"109880","DOI":"10.1016\/j.lwt.2020.109880","article-title":"Current Trends in Applications of Enzymatic Interesterification of Fats and Oils: A Review","volume":"132","author":"Sivakanthan","year":"2020","journal-title":"LWT"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1113","DOI":"10.1017\/S000711451500272X","article-title":"Vitamin E Function and Requirements in Relation to PUFA","volume":"114","author":"Raederstorff","year":"2015","journal-title":"Br. J. Nutr."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1281","DOI":"10.1007\/s00394-016-1178-0","article-title":"Plasma N-3 Polyunsaturated Fatty Status and Its Relationship with Vitamin E Intake and Plasma Level","volume":"56","author":"Zhao","year":"2017","journal-title":"Eur. J. Nutr."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1016\/S0076-6879(97)86025-9","article-title":"Lipases in Supercritical Fluids","volume":"Volume 286","author":"Cernia","year":"1997","journal-title":"Methods in Enzymology"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"331","DOI":"10.3390\/life4030331","article-title":"Supercritical Carbon Dioxide and Its Potential as a Life-Sustaining Solvent in a Planetary Environment","volume":"4","author":"Budisa","year":"2014","journal-title":"Life"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Claria, B., Espinosa, A., Rodr\u00edguez, A., Dovale-Rosabal, G., Bucarey, J.L., Pando, M.E., Romero, N., Reinoso, F., S\u00e1nchez, C., and Valenzuela, R. (2024). Cold-Pressed Aristotelia Chilensis (Mol.) Stuntz Seed Oil Prevents Metabolic-Dysfunction-Associated Steatotic Liver Disease (MASLD) in a High-Fat-Diet-Induced Obesity Murine Model. Antioxidants, 13.","DOI":"10.3390\/antiox13111384"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Reinoso, F., Rodr\u00edguez, A., S\u00e1nchez, C., Claria, B., Romero, N., Espinosa, A., Pando, M.E., Valenzuela, R., Apaza, D., and Dovale-Rosabal, G. (2024). Enzymatic Interesterification of Cold-Pressed Maqui (Aristotelia chilensis (Mol.) Stuntz) Seed Oil and Belly Oil from Rainbow Trout (Oncorhynchus mykiss) Through Supercritical CO2. Mar. Drugs, 22.","DOI":"10.3390\/md22120547"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1016\/S0076-6879(81)72003-2","article-title":"Extraction of Tissue Lipids with a Solvent of Low Toxicity","volume":"Volume 72","author":"Radin","year":"1981","journal-title":"Methods in Enzymology"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1007\/s00217-020-03643-4","article-title":"Acylglycerol Synthesis Including EPA and DHA from Rainbow Trout (Oncorhynchus mykiss) Belly Flap Oil and Caprylic Acid Catalyzed by Thermomyces Lanuginosus Lipase under Supercritical Carbon Dioxide","volume":"247","author":"Pando","year":"2021","journal-title":"Eur. Food Res. Technol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1007\/s11746-003-0799-5","article-title":"Concentrating PUFA from Mackerel Processing Waste","volume":"80","author":"Zuta","year":"2003","journal-title":"J. Amer. Oil Chem. Soc."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1007\/s11746-001-0289-9","article-title":"Purification Process for Cod Liver Oil Polyunsaturated Fatty Acids","volume":"78","author":"Belarbi","year":"2001","journal-title":"J. Amer. Oil Chem. Soc."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.foodchem.2017.01.123","article-title":"Identification and Quantification of Ethyl Carbamate Occurring in Urea Complexation Processes Commonly Utilized for Polyunsaturated Fatty Acid Concentration","volume":"229","author":"Prados","year":"2017","journal-title":"Food Chem."},{"key":"ref_27","unstructured":"Myers, R.H., Montgomery, D.C., and Anderson-Cook, C.M. (2009). Response Surface Methodology: Process and Product Optimization Using Designed Experiments, John Wiley & Sons."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"571","DOI":"10.2307\/1266632","article-title":"A Review of Response Surface Methodology: A Literature Survey","volume":"8","author":"Hill","year":"1966","journal-title":"Technometrics"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/j.jfoodeng.2005.01.020","article-title":"Concentration of Docosahexaenoic Acid (DHA) and Eicosapentaenoic Acid (EPA) of Tuna Oil by Urea Complexation: Optimization of Process Parameters","volume":"73","author":"Liu","year":"2006","journal-title":"J. Food Eng."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Dovale-Rosabal, G., Espinosa, A., Rodr\u00edguez, A., Barriga, A., Palomino-Calder\u00f3n, A., Romero, N., Troncoso, R.H., and Aubourg, S.P. (2022). Effect of Structured Phenolic Lipids with EPA\/DHA and Gallic Acid against Metabolic-Associated Fatty Liver Disease (MAFLD) in Mice. Molecules, 27.","DOI":"10.3390\/molecules27227702"},{"key":"ref_31","unstructured":"IUPAC Standard Method 2.301 (1987). Preparation of Fatty Acid Methyl Esters. Standard Methods for Analysis of Oils, Fats and Derivatives, Blackwell Scientific Publications. [7th ed.]."},{"key":"ref_32","unstructured":"American Oil Chemists\u2019 Society (AOCS) (2017). Determination of cis-, trans-, saturated, monounsaturated, and polyunsaturated fatty acids by capillary gas liquid chromatography (GLC). Sampling and analysis of commercial fats and oils. Official Methods and Recommended Practices of the American Oil Chemists\u2019 Society, AOCS. [7th ed.]. Official Method Ce 1j-7."},{"key":"ref_33","unstructured":"American Oil Chemists\u2019 Society (AOCS) (1997). Determination of tocopherols and tocotrienols in vegetable oils and fats by HPLC. Official Methods and Recommended Practices of the American Oil Chemists Society, AOCS. [5th ed.]. Official method Ce 8-89."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1080\/10242420701379916","article-title":"Lipase-Catalyzed Synthesis of Phenolic Lipids from Fish Liver Oil and Dihydrocaffeic Acid","volume":"25","author":"Sabally","year":"2007","journal-title":"Biocatal. Biotransformation"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"2754","DOI":"10.1016\/j.chroma.2010.11.066","article-title":"Lipid Analysis by Thin-Layer Chromatography\u2014A Review of the Current State","volume":"1218","author":"Fuchs","year":"2011","journal-title":"J. Chromatogr"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1111\/j.1745-4522.1997.tb00080.x","article-title":"Positional Distribution of Fatty Acids in Triacylglycerols of Seal Blubber Oil","volume":"4","author":"Wanasundara","year":"1997","journal-title":"J. Food Lipids"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1007\/s11746-001-0280-5","article-title":"Interesterification of Tallow and Sunflower Oil","volume":"78","author":"Castro","year":"2001","journal-title":"J. Am. Oil Chem. Soc."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"2071","DOI":"10.1021\/jf001072c","article-title":"Synthesis of Structured Lipids by Transesterification of Trilinolein Catalyzed by Lipozyme IM60","volume":"49","author":"Sellappan","year":"2001","journal-title":"J. Agric. Food Chem."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"857","DOI":"10.1007\/s12272-021-01350-6","article-title":"Mouse Model of the Adipose Organ: The Heterogeneous Anatomical Characteristics","volume":"44","author":"Chun","year":"2021","journal-title":"Arch. Pharm. Res."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"B\u00f6rgeson, E., Boucher, J., and Hagberg, C.E. (2022). Of Mice and Men: Pinpointing Species Differences in Adipose Tissue Biology. Front. Cell Dev. Biol., 10.","DOI":"10.3389\/fcell.2022.1003118"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Liang, W., Menke, A.L., Driessen, A., Koek, G.H., Lindeman, J.H., Stoop, R., Havekes, L.M., Kleemann, R., and van den Hoek, A.M. (2014). Establishment of a General NAFLD Scoring System for Rodent Models and Comparison to Human Liver Pathology. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0115922"},{"key":"ref_42","first-page":"71","article-title":"An Improvement of the 2\u02c6(-Delta Delta CT) Method for Quantitative Real-Time Polymerase Chain Reaction Data Analysis","volume":"3","author":"Rao","year":"2013","journal-title":"Biostat Bioinform. Biomath"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"233","DOI":"10.3390\/ijms11010233","article-title":"A Review on the Effects of Supercritical Carbon Dioxide on Enzyme Activity","volume":"11","author":"Wimmer","year":"2010","journal-title":"Int. J. Mol. Sci."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1016\/S0141-0229(98)00164-1","article-title":"A Study of Hydrolases Stability in Supercritical Carbon Dioxide (SC-CO2)","volume":"24","author":"Magor","year":"1999","journal-title":"Enzym. Microb. Technol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1016\/j.supflu.2006.03.020","article-title":"Supercritical Fluid Extraction and Fractionation of Natural Matter","volume":"38","author":"Reverchon","year":"2006","journal-title":"J. Supercrit. Fluids"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1080\/19390210902861841","article-title":"The Essentials of Essential Fatty Acids","volume":"6","year":"2009","journal-title":"J. Diet. Suppl."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"23857","DOI":"10.1007\/s13399-023-04422-3","article-title":"Development of Omega-3-Rich Structured Lipids Using Perilla Seed Oil and Palm Olein: Optimization and Characterization","volume":"14","author":"Singh","year":"2024","journal-title":"Biomass Conv. Bioref."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"111036","DOI":"10.1016\/j.fct.2019.111036","article-title":"Chemoprotective and Antiobesity Effects of Tocols from Seed Oil of Maqui-Berry: Their Antioxidative and Digestive Enzyme Inhibition Potential","volume":"136","author":"Monterrosa","year":"2020","journal-title":"Food Chem. Toxicol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1016\/j.supflu.2009.03.010","article-title":"Supercritical CO2 Extraction of Omega-3 Rich Oil from Sacha Inchi (Plukenetia volubilis L.) Seeds","volume":"49","author":"Piantino","year":"2009","journal-title":"J. Supercrit. Fluids"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"105121","DOI":"10.1016\/j.supflu.2020.105121","article-title":"Supercritical CO2 Processing of Omega-3 Polyunsaturated Fatty Acids\u2014Towards a Biorefinery for Fish Waste Valorization","volume":"169","author":"Melgosa","year":"2021","journal-title":"J. Supercrit. Fluids"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1007\/s11746-005-1135-9","article-title":"Structured Lipids from High-Laurate Canola Oil and Long-Chain Omega-3 Fatty Acids","volume":"82","author":"Hamam","year":"2005","journal-title":"J. Am. Oil Chem. Soc."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Dovale-Rosabal, G., Rodr\u00edguez, A., Espinosa, A., Barriga, A., and Aubourg, S.P. (2021). Synthesis of EPA- and DHA-Enriched Structured Acylglycerols at the Sn-2 Position Starting from Commercial Salmon Oil by Enzymatic Lipase Catalysis under Supercritical Conditions. Molecules, 26.","DOI":"10.3390\/molecules26113094"},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Cholewski, M., Tomczykowa, M., and Tomczyk, M. (2018). A Comprehensive Review of Chemistry, Sources and Bioavailability of Omega-3 Fatty Acids. Nutrients, 10.","DOI":"10.3390\/nu10111662"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"4516","DOI":"10.1002\/fsn3.3410","article-title":"Effects of a Novel Medium\u2013Long\u2013Medium-Type Structured Lipid Synthesized Using a Two-Step Enzymatic Method on Lipid Metabolism and Obesity Protection in Experimental Mice","volume":"11","author":"Zhou","year":"2023","journal-title":"Food Sci. Nutr."},{"key":"ref_55","first-page":"353","article-title":"Enzymatic Synthesis of Structured Lipids with Behenic Acid at the Sn-1, 3 Positions of Triacylglycerols","volume":"27","author":"Kok","year":"2017","journal-title":"Food Sci. Biotechnol"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"106354","DOI":"10.1016\/j.ultsonch.2023.106354","article-title":"Preparation and Characterization of Diacylglycerol via Ultrasound-Assisted Enzyme-Catalyzed Transesterification of Lard with Glycerol Monolaurate","volume":"95","author":"Diao","year":"2023","journal-title":"Ultrason. Sonochem"},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Ji, T., Fang, B., Wu, F., Liu, Y., Cheng, L., Li, Y., Wang, R., and Zhu, L. (2023). Diet Change Improves Obesity and Lipid Deposition in High-Fat Diet-Induced Mice. Nutrients, 15.","DOI":"10.3390\/nu15234978"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1146\/annurev-pathol-121808-102132","article-title":"Nonalcoholic Fatty Liver Disease: Pathology and Pathogenesis","volume":"5","author":"Tiniakos","year":"2010","journal-title":"Annu. Rev. Pathol. Mech. Dis."},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Valenzuela, R., Espinosa, A., Gonz\u00e1lez-Ma\u00f1\u00e1n, D., D\u2019Espessailles, A., Fern\u00e1ndez, V., Videla, L.A., and Tapia, G. (2012). N-3 Long-Chain Polyunsaturated Fatty Acid Supplementation Significantly Reduces Liver Oxidative Stress in High Fat Induced Steatosis. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0046400"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"461","DOI":"10.3748\/wjg.v24.i4.461","article-title":"Diet Switch and Omega-3 Hydroxy-Fatty Acids Display Differential Hepatoprotective Effects in an Obesity\/Nonalcoholic Fatty Liver Disease Model in Mice","volume":"24","year":"2018","journal-title":"World J. Gastroenterol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1039\/C5FO01086A","article-title":"Anti-Steatotic Effects of an n-3 LCPUFA and Extra Virgin Olive Oil Mixture in the Liver of Mice Subjected to High-Fat Diet","volume":"7","author":"Valenzuela","year":"2016","journal-title":"Food Funct."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"110602","DOI":"10.1016\/j.foodres.2021.110602","article-title":"Enzymatic Synthesis of Capric Acid-Rich Structured Lipids and Their Effects on Mice with High-Fat Diet-Induced Obesity","volume":"148","author":"Mesa","year":"2021","journal-title":"Food Res. Int."},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"la Fuente, F.P., Quezada, L., Sep\u00falveda, C., Monsalves-Alvarez, M., Rodr\u00edguez, J.M., Sacrist\u00e1n, C., Chiong, M., Llanos, M., Espinosa, A., and Troncoso, R. (2019). Exercise Regulates Lipid Droplet Dynamics in Normal and Fatty Liver. Biochim. Biophys. Acta Mol. Cell Biol. Lipids, 1864.","DOI":"10.1016\/j.bbalip.2019.158519"},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Gaggini, M., Gorini, F., and Vassalle, C. (2022). Lipids in Atherosclerosis: Pathophysiology and the Role of Calculated Lipid Indices in Assessing Cardiovascular Risk in Patients with Hyperlipidemia. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24010075"},{"key":"ref_65","first-page":"e673","article-title":"Omega-3 Fatty Acids for the Management of Hypertriglyceridemia: A Science Advisory from the American Heart Association","volume":"140","author":"Wilson","year":"2019","journal-title":"Circulation"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/B978-012369454-6\/50060-1","article-title":"Clinical Chemistry of the Laboratory Mouse","volume":"3","author":"Quimby","year":"2007","journal-title":"Mouse Biomed. Res."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"2884","DOI":"10.1093\/cvr\/cvad188","article-title":"Do Patients Benefit from Omega-3 Fatty Acids?","volume":"119","author":"Sherratt","year":"2024","journal-title":"Cardiovasc. Res."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"102456","DOI":"10.1016\/j.plefa.2022.102456","article-title":"The Effects of Omega-3 Fatty Acids in Type 2 Diabetes: A Systematic Review and Meta-Analysis","volume":"182","author":"Xiao","year":"2022","journal-title":"Prostaglandins leukot. Essent. Fat. Acids"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1017\/S0007114507207627","article-title":"Eicosapentaenoic Acid Actions on Adiposity and Insulin Resistance in Control and High-Fat-Fed Rats: Role of Apoptosis, Adiponectin and Tumour Necrosis Factor-Alpha","volume":"97","author":"Marti","year":"2007","journal-title":"Br. J. Nutr."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"R25","DOI":"10.1530\/JOE-13-0201","article-title":"Inflammation as a Potential Link between Nonalcoholic Fatty Liver Disease and Insulin Resistance","volume":"218","author":"Asrih","year":"2013","journal-title":"J. Endocrinol."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"3878","DOI":"10.1038\/ncomms4878","article-title":"Hepatocyte Toll-like Receptor 4 Regulates Obesity-Induced Inflammation and Insulin Resistance","volume":"5","author":"Jia","year":"2014","journal-title":"Nat. Commun."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"G270","DOI":"10.1152\/ajpgi.00304.2014","article-title":"Hepatic TLR4 Signaling in Obese NAFLD","volume":"309","author":"Sharifnia","year":"2015","journal-title":"Am. J. Physiol. Gastrointest. Liver Physiol."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"658","DOI":"10.1016\/S1262-3636(08)74601-9","article-title":"Adipose Tissue Inflammation and Liver Pathology in Human Obesity","volume":"34","author":"Tordjman","year":"2008","journal-title":"Diabetes Metab."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1017\/S0029665120007077","article-title":"N-3 PUFA and Inflammation: From Membrane to Nucleus and from Bench to Bedside","volume":"79","author":"Calder","year":"2020","journal-title":"Proc. Nutr. Soc."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1097\/MCO.0000000000000539","article-title":"Omega-3 Fatty Acids and Nonalcoholic Fatty Liver Disease in Adults and Children: Where Do We Stand?","volume":"22","author":"Spooner","year":"2019","journal-title":"Curr. Opin. Clin. Nutr. Metab. Care"},{"key":"ref_76","doi-asserted-by":"crossref","unstructured":"Depner, C.M., Traber, M.G., Bobe, G., Kensicki, E., Bohren, K.M., Milne, G., and Jump, D.B. (2013). A Metabolomic Analysis of Omega-3 Fatty Acid-Mediated Attenuation of Western Diet-Induced Nonalcoholic Steatohepatitis in LDLR-\/- Mice. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0083756"},{"key":"ref_77","doi-asserted-by":"crossref","unstructured":"\u0160m\u00edd, V., Dvo\u0159\u00e1k, K., Stehnov\u00e1, K., Strnad, H., Rubert, J., St\u0159\u00edtesk\u00fd, J., Sta\u0148kov\u00e1, B., Str\u00e1nsk\u00e1, M., Haj\u0161lov\u00e1, J., and Br\u016fha, R. (2023). The Ameliorating Effects of N-3 Polyunsaturated Fatty Acids on Liver Steatosis Induced by a High-Fat Methionine Choline-Deficient Diet in Mice. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms242417226"},{"key":"ref_78","doi-asserted-by":"crossref","unstructured":"Todor\u010devi\u0107, M., and Hodson, L. (2015). The Effect of Marine Derived N-3 Fatty Acids on Adipose Tissue Metabolism and Function. J. Clin. Med., 5.","DOI":"10.3390\/jcm5010003"},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"140","DOI":"10.3164\/jcbn.21-69","article-title":"Effect of Tocotrienol on the Primary Progression of Nonalcoholic Steatohepatitis in a Mouse Model","volume":"70","author":"Noichi","year":"2022","journal-title":"J. Clin. Biochem. Nutr."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.nut.2018.01.012","article-title":"Role of Dietary \u03b1- and \u03b3-Tocopherol from Rosa Mosqueta Oil in the Prevention of Alterations Induced by High-Fat Diet in a Murine Model","volume":"53","author":"Tapia","year":"2018","journal-title":"Nutrition"},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"6501046","DOI":"10.1155\/2017\/6501046","article-title":"Measurement and Clinical Significance of Biomarkers of Oxidative Stress in Humans","volume":"2017","author":"Marrocco","year":"2017","journal-title":"Oxid. Med. Cell Longev."},{"key":"ref_82","doi-asserted-by":"crossref","unstructured":"Hou, Z., Zhu, L., Meng, R., and Wang, B. (2020). Hypolipidemic and Antioxidant Activities of Trichosanthes Kirilowii Maxim Seed Oil and Flavonoids in Mice Fed with a High-Fat Diet. J. Food Biochem., 44.","DOI":"10.1111\/jfbc.13272"}],"container-title":["Antioxidants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-3921\/14\/7\/790\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T17:59:31Z","timestamp":1760032771000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-3921\/14\/7\/790"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,26]]},"references-count":82,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2025,7]]}},"alternative-id":["antiox14070790"],"URL":"https:\/\/doi.org\/10.3390\/antiox14070790","relation":{},"ISSN":["2076-3921"],"issn-type":[{"value":"2076-3921","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,6,26]]}}}