{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:31:55Z","timestamp":1787027515889,"version":"build-2736575974"},"reference-count":116,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2025,7,11]],"date-time":"2025-07-11T00:00:00Z","timestamp":1752192000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Science and technology project of Yili in Xinjiang","award":["YZD2024A08"],"award-info":[{"award-number":["YZD2024A08"]}]},{"name":"Science and technology project of Yili in Xinjiang","award":["2022AB012"],"award-info":[{"award-number":["2022AB012"]}]},{"name":"Science and technology project of Yili in Xinjiang","award":["2021AA004"],"award-info":[{"award-number":["2021AA004"]}]},{"name":"Science and technology project of Yili in Xinjiang","award":["2020CB023"],"award-info":[{"award-number":["2020CB023"]}]},{"name":"Bingtuan science and technology program","award":["YZD2024A08"],"award-info":[{"award-number":["YZD2024A08"]}]},{"name":"Bingtuan science and technology program","award":["2022AB012"],"award-info":[{"award-number":["2022AB012"]}]},{"name":"Bingtuan science and technology program","award":["2021AA004"],"award-info":[{"award-number":["2021AA004"]}]},{"name":"Bingtuan science and technology program","award":["2020CB023"],"award-info":[{"award-number":["2020CB023"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>This study evaluated the inhibitory activity of quercetin at sub-inhibitory concentrations on quorum-sensing (QS) molecules in vitro and the effects of dietary supplementation with quercetin (for 24 consecutive days) on enterotoxigenic Escherichia coli (ETEC)-induced inflammatory and oxidative stress responses in weaned piglets. The piglets were fed one of three diets: the basal diet (Con), ETEC challenge (K88) after the basal diet, or ETEC challenge (quercetin + K88) after the basal diet supplemented with 0.2% quercetin. In vitro experiments revealed that 5 mg\/mL quercetin exhibited the strongest QS inhibitory activity and reduced pigment production by Chromobacterium violaceum ATCC12472 by 67.70%. In vivo experiments revealed that quercetin + K88 significantly increased immunoglobulin A (IgA), immunoglobulin M (IgM), and immunoglobulin G (IgG) levels in the serum, ileum mucosa, and colon mucosa; increased glutathione peroxidase (GSH-Px), catalase (CAT), and superoxide dismutase (SOD) levels in the serum, liver, and colon mucosa; and decreased cluster of differentiation 3 (CD3) and cluster of differentiation 8 (CD8)activity in the serum compared with K88 alone. Quercetin + K88 significantly alleviated pathological damage to the liver and spleen and upregulated antioxidant genes (nuclear factor erythroid 2-related factor 2 (Nrf2), heme oxygenase-1(HO-1), CAT, SOD, and glutathione s-transferase (GST)). Inducible nitric oxide synthase (iNOS) and kelch-like ech-associated protein 1 (Keap1), which cause oxidative damage to the liver and spleen, were significantly downregulated. The acetic acid content in the cecum was significantly increased, and the E. coli count and QS signal molecule autoinducer-2 (AI-2) yield were significantly reduced. In conclusion, 0.2% dietary quercetin can alleviate ETEC-induced inflammation and oxidative stress in weaned piglets.<\/jats:p>","DOI":"10.3390\/antiox14070852","type":"journal-article","created":{"date-parts":[[2025,7,11]],"date-time":"2025-07-11T15:19:31Z","timestamp":1752247171000},"page":"852","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Quercetin Can Alleviate ETECK88-Induced Oxidative Stress in Weaned Piglets by Inhibiting Quorum-Sensing Signal Molecule Autoinducer-2 Production in the Cecum"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-9311-8212","authenticated-orcid":false,"given":"Hailiang","family":"Wang","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology, Shihezi University, Shihezi 832000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Yao","sequence":"additional","affiliation":[{"name":"School of Medicine, Shihezi University, Shihezi 832000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Wang","sequence":"additional","affiliation":[{"name":"Animal Husbandry and Fishery Development Service Center, Shihezi 832000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingyang","family":"Geng","sequence":"additional","affiliation":[{"name":"Institute of Animal Science of Ili Kazakh Autonomous Prefecture, Yining 835000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanshan","family":"Nan","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Shihezi University, Shihezi 832000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangjian","family":"Peng","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Shihezi University, Shihezi 832000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuyang","family":"Xue","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Shihezi University, Shihezi 832000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5314-9904","authenticated-orcid":false,"given":"Wenju","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Shihezi University, Shihezi 832000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3320-2840","authenticated-orcid":false,"given":"Cunxi","family":"Nie","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Shihezi University, Shihezi 832000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e321","DOI":"10.1016\/S2214-109X(18)30483-2","article-title":"Burden of enterotoxigenic Escherichia coli and shigella non-fatal diarrhoeal infections in 79 low-income and lower middle-income countries: A modelling analysis","volume":"7","author":"Anderson","year":"2019","journal-title":"Lancet Glob. Health"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"e101","DOI":"10.1016\/S2214-109X(19)30456-5","article-title":"Heterogeneity in enterotoxigenic Escherichia coli and shigella infections in children under 5 years of age from 11 African countries: A subnational approach quantifying risk, mortality, morbidity, and stunting","volume":"8","author":"Bagamian","year":"2020","journal-title":"Lancet Glob. Health"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Dubreuil, J.D., Isaacson, R.E., and Schifferli, D.M. (2016). Animal Enterotoxigenic Escherichia coli. EcoSal Plus, 7.","DOI":"10.1128\/ecosalplus.esp-0006-2016"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Barros, M.M., Castro, J., Ara\u00fajo, D., Campos, A.M., Oliveira, R., Silva, S., Outor-Monteiro, D., and Almeida, C. (2023). Swine Colibacillosis: Global Epidemiologic and Antimicrobial Scenario. Antibiotics, 12.","DOI":"10.3390\/antibiotics12040682"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1186\/s13567-016-0313-5","article-title":"Porcine aminopeptidase N binds to F4+ enterotoxigenic Escherichia coli fimbriae","volume":"47","author":"Xia","year":"2016","journal-title":"Vet. Res."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1018","DOI":"10.1096\/fj.201901717R","article-title":"Therapeutic administration of the recombinant antimicrobial peptide microcin J25 effectively enhances host defenses against gut inflammation and epithelial barrier injury induced by enterotoxigenic Escherichia coli infection","volume":"34","author":"Yu","year":"2020","journal-title":"FASEB J."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Poirel, L., Madec, J., Lupo, A., Schink, A., Kieffer, N., Nordmann, P., and Schwarz, S. (2018). Antimicrobial Resistance in Escherichia coli. Microbiol. Spectr., 6.","DOI":"10.1128\/microbiolspec.ARBA-0026-2017"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1016\/j.envres.2018.11.040","article-title":"Human health risk assessment of antibiotic resistance associated with antibiotic residues in the environment: A review","volume":"169","author":"Ben","year":"2019","journal-title":"Environ. Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"106473","DOI":"10.1016\/j.ejps.2023.106473","article-title":"European regulations on the use of antibiotics in veterinary medicine","volume":"189","author":"Schmerold","year":"2023","journal-title":"Eur. J. Pharm. Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1649","DOI":"10.1016\/j.clinthera.2020.07.004","article-title":"Antibiotic Stewardship in Food-producing Animals: Challenges, Progress, and Opportunities","volume":"42","author":"Patel","year":"2020","journal-title":"Clin. Ther."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/j.tvjl.2010.05.001","article-title":"The European ban on antibiotic growth promoters in animal feed: From challenges to opportunities","volume":"187","author":"Millet","year":"2011","journal-title":"Vet. J."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1038\/nature24624","article-title":"Progress in and promise of bacterial quorum sensing research","volume":"551","author":"Whiteley","year":"2017","journal-title":"Nature"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1080\/14787210.2020.1750951","article-title":"Alternative approaches to treat bacterial infections: Targeting quorum-sensing","volume":"18","author":"Piewngam","year":"2020","journal-title":"Expert. Rev. Anti Infect. Ther."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1328.e1","DOI":"10.1016\/j.cell.2018.08.003","article-title":"SnapShot: Bacterial Quorum Sensing","volume":"174","author":"Eickhoff","year":"2018","journal-title":"Cell"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Quan, Y., Meng, F., Ma, X., Song, X., Liu, X., Gao, W., Dang, Y., Meng, Y., Cao, M., and Song, C. (2017). Regulation of bacteria population behaviors by AI-2 \u201cconsumer cells\u201d and \u201csupplier cells\u201d. BMC Microbiol., 17.","DOI":"10.1186\/s12866-017-1107-2"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Song, S., and Wood, T.K. (2021). The Primary Physiological Roles of Autoinducer 2 in Escherichia coli Are Chemotaxis and Biofilm Formation. Microorganisms, 9.","DOI":"10.3390\/microorganisms9020386"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1016\/j.micpath.2018.05.046","article-title":"Developments in strategies for Quorum Sensing virulence factor inhibition to combat bacterial drug resistance","volume":"121","author":"Haque","year":"2018","journal-title":"Microb. Pathog."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Zhao, X., Yu, Z., and Ding, T. (2020). Quorum-Sensing Regulation of Antimicrobial Resistance in Bacteria. Microorganisms, 8.","DOI":"10.3390\/microorganisms8030425"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"674","DOI":"10.1111\/1751-7915.12730","article-title":"Adjuncts and alternatives in the time of antibiotic resistance and in-feed antibiotic bans","volume":"10","year":"2017","journal-title":"Microb. Biotechnol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1111\/jpn.13922","article-title":"Assessing the potential of phytogenic feed additives: A comprehensive review on their effectiveness as a potent dietary enhancement for nonruminant in swine and poultry","volume":"108","author":"Biswas","year":"2024","journal-title":"J. Anim. Physiol. Anim. Nutr."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Alymanesh, M.R., Solhjoo, A., Pishgarm, E., and Akhlaghi, M. (2024). Falcaria vulgaris extract: A mixture of quorum sensing inhibitors for controlling Pectobacterium carotovorum subsp. carotovorum. Food Microbiol., 122.","DOI":"10.1016\/j.fm.2024.104535"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"El-Sayed, N.R., Samir, R., Jamil, M., Abdel-Hafez, L., and Ramadan, M.A. (2020). Olive Leaf Extract Modulates Quorum Sensing Genes and Biofilm Formation in Multi-DrugResistant Pseudomonas aeruginosa. Antibiotics, 9.","DOI":"10.3390\/antibiotics9090526"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Husain, F.M., Ahmad, I., Al-Thubiani, A.S., Abulreesh, H.H., AlHazza, I.M., and Aqil, F. (2017). Leaf Extracts of Mangifera indica L. Inhibit Quorum Sensing\u2014Regulated Production of Virulence Factors and Biofilm in Test Bacteria. Front. Microbiol., 8.","DOI":"10.3389\/fmicb.2017.00727"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Mostafa, I., Abbas, H.A., Ashour, M.L., Yasri, A., El-Shazly, A.M., Wink, M., and Sobeh, M. (2020). Polyphenols from Salix tetrasperma Impair Virulence and Inhibit Quorum Sensing of Pseudomonas aeruginosa. Molecules, 25.","DOI":"10.3390\/molecules25061341"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1477","DOI":"10.1111\/1750-3841.14648","article-title":"Effect of Capsicum Frutescens Extract, Capsaicin, and Luteolin on Quorum Sensing Regulated Phenotypes","volume":"84","author":"Rivera","year":"2019","journal-title":"J. Food Sci."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Quecan, B.X.V., Santos, J.T.C., Rivera, M.L.C., Hassimotto, N.M.A., Almeida, F.A., and Pinto, U.M. (2019). Effect of quercetin Rich Onion Extracts on Bacterial Quorum Sensing. Front. Microbiol., 10.","DOI":"10.3389\/fmicb.2019.00867"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1007\/s00894-020-04356-x","article-title":"Mechanism of antioxidant properties of quercetin and quercetin-DNA complex","volume":"26","author":"Song","year":"2020","journal-title":"J. Mol. Model."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"105676","DOI":"10.1016\/j.intimp.2019.105676","article-title":"Anti-inflammatory effects of quercetin in a mouse model of MC903-induced atopic dermatitis","volume":"74","author":"Hou","year":"2019","journal-title":"Int. Immunopharmacol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1007\/s00289-022-04091-8","article-title":"An insight into anticancer, antioxidant, antimicrobial, antidiabetic and anti-inflammatory effects of quercetin: A review","volume":"80","author":"Azeem","year":"2023","journal-title":"Polym. Bull."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"107","DOI":"10.2527\/asasmw.2017.12.224","article-title":"Effects of mixture of organic acids and medium chain fatty acids on growth performance, diarrhea incidence, and fecal microbial flora in weaning pigs orally challenged with enterotoxigenic Escherichia coli K88","volume":"95","author":"Nguyen","year":"2024","journal-title":"J. Anim. Sci."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Vangroenweghe, F.A. (2021). Vaccination with an E. coli F4\/F18 vaccine for the prevention of F4-ETEC post-weaning diarrhea resulted in reduced post-weaning mortality and antibiotic use. J. Anim. Sci., 99.","DOI":"10.1093\/jas\/skab054.236"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.rvsc.2023.02.011","article-title":"Effects on the intestinal morphology, inflammatory response and microflora in piglets challenged with enterotoxigenic Escherichia coli K88","volume":"157","author":"Zhang","year":"2023","journal-title":"Res. Vet. Sci."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Jin, S., Xu, H., and Yang, C.O.K. (2024). Regulation of oxidative stress in the intestine of piglets after enterotoxigenic Escherichia coli (ETEC) infection. Biochim. Biophys. Acta Mol. Cell Res., 871.","DOI":"10.1016\/j.bbamcr.2024.119711"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"5508","DOI":"10.1080\/14786419.2020.1788554","article-title":"Amicoumacins from a desert bacterium: Quorum sensing inhibitor against Chromobacterium violaceum","volume":"35","author":"Shi","year":"2021","journal-title":"Nat. Prod. Res."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Skogman, M.E., Kanerva, S., Manner, S., Vuorela, P.M., and Fallarero, A. (2016). Flavones as Quorum Sensing Inhibitors Identified by a Newly Optimized Screening Platform Using Chromobacterium violaceum as Reporter Bacteria. Molecules, 21.","DOI":"10.3390\/molecules21091211"},{"key":"ref_36","unstructured":"National Research Council (2012). Nutrient Requirements of Swine."},{"key":"ref_37","unstructured":"(2018). Determination of Crude Protein in Feeds\u2014Kjeldahl Method (Standard No. GB\/T 6432-2018)."},{"key":"ref_38","unstructured":"(2018). Determination of Calcium in Feeds (Standard No. GB\/T 6436-2018)."},{"key":"ref_39","unstructured":"(2018). Determination of Phosphorus in Feeds\u2014Spectrophotometry (Standard No. GB\/T 6437-2018)."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"100037","DOI":"10.1016\/j.animal.2020.100037","article-title":"Meishan neonatal piglets tend to have higher intestinal barrier function than crossbred neonatal piglets","volume":"15","author":"Dong","year":"2021","journal-title":"Animal"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Pang, X., Wei, X., Wu, Y., Nan, S., Feng, J., Wang, F., Yao, M., and Nie, C. (2024). Capsaicin Modulates Hepatic and Intestinal Inflammation and Oxidative Stress by Regulating the Colon Microbiota. Antioxidants, 13.","DOI":"10.3390\/antiox13080942"},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Rami\u0107, D., Klan\u010dnik, A., Mo\u017eina, S.S., and Dogsa, I. (2022). Elucidation of the AI-2 communication system in the food-borne pathogen Campylobacter jejuni by whole-cell-based biosensor quantification. Biosens. Bioelectron., 212.","DOI":"10.1016\/j.bios.2022.114439"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1016\/j.tim.2013.09.006","article-title":"Quorum sensing inhibitors: How strong is the evidence?","volume":"21","author":"Defoirdt","year":"2013","journal-title":"Trends Microbiol."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Hernando-Amado, S., Alcalde-Rico, M., Gil-Gil, T., Valverde, J.R., and Mart\u00ednez, J.L. (2020). Naringenin Inhibition of the Pseudomonas aeruginosa Quorum Sensing Response Is Based on Its Time-Dependent Competition With N-(3-Oxo-dodecanoyl)-L-homoserine Lactone for LasR Binding. Front. Mol. Biosci., 7.","DOI":"10.3389\/fmolb.2020.00025"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"4064","DOI":"10.1074\/jbc.M116.770552","article-title":"Flavonoids Suppress Pseudomonas aeruginosa Virulence through Allosteric Inhibition of Quorum-sensing Receptors","volume":"292","author":"Paczkowski","year":"2017","journal-title":"J. Biol. Chem."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"7346","DOI":"10.1039\/D1FO03854K","article-title":"Resveratrol alleviates enterotoxigenic Escherichia coli K88-induced damage by regulating SIRT-1 signaling in intestinal porcine epithelial cells","volume":"13","author":"Luo","year":"2022","journal-title":"Food Funct."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.alit.2018.09.004","article-title":"comprehensive understanding of the gut mucosal immune system in allergic inflammation","volume":"68","author":"Tokuhara","year":"2019","journal-title":"Allergol. Int."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"423","DOI":"10.2183\/pjab.92.423","article-title":"Maintenance of gut homeostasis by the mucosal immune system","volume":"92","author":"Okumura","year":"2016","journal-title":"Proc. Jpn. Acad. Ser. B Phys. Biol. Sci."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1038\/s41577-019-0261-1","article-title":"Rethinking mucosal antibody responses: IgM, IgG and IgD join IgA","volume":"20","author":"Chen","year":"2016","journal-title":"Nat. Rev. Immunol."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"\u010cipak Ga\u0161parovi\u0107, A., Milkovi\u0107, L., Rodrigues, C., Mlinari\u0107, M., and Soveral, G. (2021). Peroxiporins Are Induced upon Oxidative Stress Insult and Are Associated with Oxidative Stress Resistance in Colon Cancer Cell Lines. Antioxidants, 10.","DOI":"10.3390\/antiox10111856"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"5438","DOI":"10.1021\/acs.jafc.3c00798","article-title":"Heat Stress-Induced Intestinal Barrier Impairment: Current Insights into the Aspects of Oxidative Stress and Endoplasmic Reticulum Stress","volume":"71","author":"Tang","year":"2023","journal-title":"J. Agric. Food Chem."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.cotox.2017.10.011","article-title":"Oxidative stress and acute hepatic injury","volume":"7","author":"Ramachandran","year":"2018","journal-title":"Curr. Opin. Toxicol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"5987183","DOI":"10.1155\/2016\/5987183","article-title":"Oregano Essential Oil Induces SOD1 and GSH Expression through Nrf2 Activation and Alleviates Hydrogen Peroxide-Induced Oxidative Damage in IPEC-J2 Cells","volume":"2016","author":"Zou","year":"2016","journal-title":"Oxid. Med. Cell Longev."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"682","DOI":"10.1902\/jop.2015.140561","article-title":"Evaluation of lipid peroxidation and oxidative DNA damage in patients with periodontitis and hyperlipidemia","volume":"86","author":"Bulut","year":"2015","journal-title":"J. Periodontol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"957","DOI":"10.1089\/ars.2020.8030","article-title":"The Role of Myeloperoxidase in Biomolecule Modification, Chronic Inflammation, and Disease","volume":"32","author":"Davies","year":"2020","journal-title":"Antioxid. Redox Signal."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1186\/s13065-018-0443-0","article-title":"In vitro antioxidant properties of the biflavonoid agathisflavone","volume":"12","author":"Andrade","year":"2018","journal-title":"Chem. Cent. J."},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Zeng, Y., Song, J., Zhang, M., Wang, H., Zhang, Y., and Suo, H. (2020). Comparison of In Vitro and In Vivo Antioxidant Activities of Six Flavonoids with Similar Structures. Antioxidants, 9.","DOI":"10.3390\/antiox9080732"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1007\/s10735-025-10411-9","article-title":"Quercetin prevents sarcopenia by reversing oxidative stress and mitochondrial damage","volume":"56","author":"Sun","year":"2025","journal-title":"J. Mol. Histol."},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Xu, D., Hu, M.J., Wang, Y.Q., and Cui, Y.L. (2019). Antioxidant Activities of Quercetin and Its Complexes for Medicinal Application. Molecules, 24.","DOI":"10.3390\/molecules24061123"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"3433","DOI":"10.1021\/acs.jafc.2c07939","article-title":"Inflammation Intensifies Monocrotaline-Induced Liver Injury","volume":"71","author":"Gong","year":"2023","journal-title":"J. Agric. Food Chem."},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Wang, Q., Ying, J., Zou, P., Zhou, Y., Wang, B., Yu, D., Li, W., and Zhan, X. (2020). Effects of Dietary Supplementation of Humic Acid Sodium and Zinc Oxide on Growth Performance, Immune Status and Antioxidant Capacity of Weaned Piglets. Animals, 10.","DOI":"10.3390\/ani10112104"},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Fong, F.L.Y., El-Nezami, H., Mykk\u00e4nen, O., and Kirjavainen, P.V. (2022). The Effects of Single Strains and Mixtures of Probiotic Bacteria on Immune Profile in Liver, Spleen, and Peripheral Blood. Front. Nutr., 9.","DOI":"10.3389\/fnut.2022.773298"},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Duan, G., Huang, P., Zheng, C., Zheng, J., Yu, J., Zhang, P., Wan, M., Li, F., Guo, Q., and Yin, Y. (2023). Development and Recovery of Liver Injury in Piglets by Incremental Injection of LPS. Antioxidants, 12.","DOI":"10.3390\/antiox12061143"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"1483","DOI":"10.1038\/s41467-021-21737-9","article-title":"Cytotoxic CD8+ T cells promote granzyme B-dependent adverse post-ischemic cardiac remodeling","volume":"12","author":"Zlatanova","year":"2021","journal-title":"Nat. Commun."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"110072","DOI":"10.1016\/j.vetimm.2020.110072","article-title":"Impact of birth weight and neonatal nutritional interventions with micronutrients and bovine colostrum on the development of piglet immune response during the peri-weaning period","volume":"226","author":"Verso","year":"2020","journal-title":"Vet. Immunol. Immunopathol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1111\/jpn.12490","article-title":"Influence of medium-chain fatty acids and short-chain organic acids on jejunal morphology and intra-epithelial immune cells in weaned piglets","volume":"101","author":"Ferrara","year":"2017","journal-title":"J. Anim. Physiol. Anim. Nutr."},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Miura, H., Jimbo, I., Oda, M., Noguchi, M., Kawasaki, K., Osada-Oka, M., Tsukahara, T., and Inoue, R. (2022). Effect of Porcine Colostral Exosomes on T Cells in the Peripheral Blood of Suckling Piglets. Animals, 12.","DOI":"10.3390\/ani12172172"},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"Hosseinzade, A., Sadeghi, O., Naghdipour Biregani, A., Soukhtehzari, S., Brandt, G.S., and Esmaillzadeh, A. (2019). Immunomodulatory Effects of Flavonoids: Possible Induction of T CD4+ Regulatory Cells Through Suppression of mTOR Pathway Signaling Activity. Front. Immunol., 10.","DOI":"10.3389\/fimmu.2019.00051"},{"key":"ref_69","doi-asserted-by":"crossref","unstructured":"Xu, Q., Guo, J., Li, X., Wang, Y., Wang, D., Xiao, K., Zhu, H., Wang, X., Hu, C.A., and Zhang, G. (2021). Necroptosis Underlies Hepatic Damage in a Piglet Model of Lipopolysaccharide-Induced Sepsis. Front. Immunol., 12.","DOI":"10.3389\/fimmu.2021.633830"},{"key":"ref_70","doi-asserted-by":"crossref","unstructured":"Machado-Junior, P.A., Ara\u00fajo, N.P.S., Souza, A.B.F., Castro, T.F., Oliveira, M., Costa, G.P., Matos, N.A., Vieira, P.M.A., Talvani, A., and Bezerra, F.S. (2020). Protective Effects of Quercetin on Livers from Mice Exposed to Long-Term Cigarette Smoke. Biomed. Res. Int., 2020.","DOI":"10.1155\/2020\/2196207"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1080\/10520295.2023.2172610","article-title":"Quercetin Ameliorates Acute Acrylamide Induced Spleen Injury","volume":"98","author":"Shukla","year":"2023","journal-title":"Biotech. Histochem."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"1759149","DOI":"10.1155\/2019\/1759149","article-title":"A Protective Role of the NRF2-Keap1 Pathway in Maintaining Intestinal Barrier Function","volume":"2019","author":"Wen","year":"2019","journal-title":"Oxid. Med. Cell Longev."},{"key":"ref_73","doi-asserted-by":"crossref","unstructured":"Li, B., Nasser, M.I., Masood, M., Adlat, S., Huang, Y., Yang, B., Luo, C., and Jiang, N. (2020). Efficiency of Traditional Chinese medicine targeting the Nrf2\/HO-1 signaling pathway. Biomed. Pharmacother., 126.","DOI":"10.1016\/j.biopha.2020.110074"},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1017\/S0007114518001630","article-title":"Dietary curcumin supplementation attenuates inflammation, hepatic injury and oxidative damage in a rat model of intra-uterine growth retardation","volume":"120","author":"He","year":"2018","journal-title":"Br. J. Nutr."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"1211","DOI":"10.3945\/jn.114.193573","article-title":"Curcumin attenuates D-galactosamine\/lipopolysaccharide-induced liver injury and mitochondrial dysfunction in mice","volume":"144","author":"Zhang","year":"2014","journal-title":"J. Nutr."},{"key":"ref_76","doi-asserted-by":"crossref","unstructured":"Cui, K., Wang, Q., Wang, S., Diao, Q., and Zhang, N. (2019). The Facilitating Effect of Tartary Buckwheat Flavonoids and Lactobacillus plantarum on the Growth Performance, Nutrient Digestibility, Antioxidant Capacity, and Fecal Microbiota of Weaned Piglets. Animals, 9.","DOI":"10.3390\/ani9110986"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"117373","DOI":"10.1016\/j.ecoenv.2024.117373","article-title":"Eucommia ulmoides flavonoids alleviate intestinal oxidative stress damage in weaned piglets by regulating the Nrf2\/Keap1 signaling pathway","volume":"288","author":"Li","year":"2024","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_78","doi-asserted-by":"crossref","unstructured":"Romero-Dur\u00e1n, M.A., Silva-Garc\u00eda, O., Perez-Aguilar, J.M., and Baizabal-Aguirre, V.M. (2024). Mechanisms of Keap1\/Nrf2 modulation in bacterial infections: Implications in persistence and clearance. Front. Immunol., 15.","DOI":"10.3389\/fimmu.2024.1508787"},{"key":"ref_79","first-page":"101314","article-title":"Effects of dietary Quercetin on growth, antioxidant capacity, immune response and immune-related gene expression in snakehead fish, Channa argus","volume":"26","author":"Kong","year":"2022","journal-title":"Aquacult. Rep."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1186\/s13048-018-0421-0","article-title":"Quercetin increases the antioxidant capacity of the ovary in menopausal rats and in ovarian granulosa cell culture in vitro","volume":"11","author":"Wang","year":"2018","journal-title":"J. Ovarian Res."},{"key":"ref_81","doi-asserted-by":"crossref","unstructured":"Qi, W., Qi, W., Xion, D., and Long, M. (2022). Quercetin: Its Antioxidant Mechanism, Antibacterial Properties and Potential Application in Prevention and Control of Toxipathy. Molecules, 27.","DOI":"10.3390\/molecules27196545"},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"1332","DOI":"10.1016\/j.cell.2016.05.041","article-title":"From dietary fiber to host physiology: Short-chain fatty acids as key bacterial metabolites","volume":"165","author":"Koh","year":"2016","journal-title":"Cell"},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"111572","DOI":"10.1016\/j.mce.2022.111572","article-title":"Short chain fatty acids: Microbial metabolites for gut-brain axis signalling","volume":"546","author":"Collins","year":"2022","journal-title":"Mol. Cell Endocrinol."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1186\/s12964-023-01219-9","article-title":"Short-chain fatty acids in diseases","volume":"21","author":"Zhang","year":"2023","journal-title":"Cell Commun. Signal."},{"key":"ref_85","doi-asserted-by":"crossref","unstructured":"Zhang, R., Li, X., and Zhang, S. (2024). The Role of Bacteria in Central Nervous System Tumors: Opportunities and Challenges. Microorganisms, 12.","DOI":"10.3390\/microorganisms12061053"},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1159\/000492853","article-title":"Short-chain fatty acids manifest stimulative and protective effects on intestinal barrier function through the inhibition of NLRP3 inflammasome and autophagy","volume":"49","author":"Feng","year":"2018","journal-title":"Cell Physiol. Biochem."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.redox.2018.02.007","article-title":"Differential effects of short chain fatty acids on endothelial Nlrp3 inflammasome activation and neointima formation: Antioxidant action of butyrate","volume":"16","author":"Yuan","year":"2018","journal-title":"Redox Biol."},{"key":"ref_88","doi-asserted-by":"crossref","unstructured":"Wu, Y., Teng, Y., Zhang, C., Pan, Y., Zhang, Q., Zhu, X., Liu, N., Su, X., and Lin, J. (2022). The ketone body b-hydroxybutyrate alleviates CoCrMo alloy particles induced osteolysis by regulating NLRP3 inflammasome and osteoclast differentiation. J. Nanobiotechnol, 20.","DOI":"10.1186\/s12951-022-01320-0"},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1186\/s12263-019-0626-x","article-title":"Gastric infusion of short-chain fatty acids can improve intestinal barrier function in weaned piglets","volume":"14","author":"Diao","year":"2019","journal-title":"Genes. Nutr."},{"key":"ref_90","doi-asserted-by":"crossref","unstructured":"Liu, J., Liu, J., Zhou, S., Fu, Y., Yang, Q., and Li, Y. (2023). Effects of quercetin and daidzein on egg quality, lipid metabolism, and cecal short-chain fatty acids in layers. Front. Vet. Sci., 10.","DOI":"10.3389\/fvets.2023.1301542"},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"112341","DOI":"10.1016\/j.intimp.2024.112341","article-title":"Quercetin ameliorates bone loss in OVX rats by modulating the intestinal flora-SCFAs-inflammatory signaling axis","volume":"136","author":"Feng","year":"2024","journal-title":"Int. Immunopharmacol."},{"key":"ref_92","doi-asserted-by":"crossref","unstructured":"Lin, Y., Zhou, B., and Zhu, W. (2021). Pathogenic Escherichia coli-Specific Bacteriophages and Polyvalent Bacteriophages in Piglet Guts with Increasing Coliphage Numbers after Weaning. Appl. Environ. Microbiol., 87.","DOI":"10.1128\/AEM.00966-21"},{"key":"ref_93","doi-asserted-by":"crossref","unstructured":"Zeng, Y., Li, R., Dong, Y., Yi, D., Wu, T., Wang, L., Zhao, D., Zhang, Y., and Hou, Y. (2023). Dietary Supplementation with Puerarin Improves Intestinal Function in Piglets Challenged with Escherichia coli K88. Animals, 13.","DOI":"10.3390\/ani13121908"},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1080\/1745039X.2020.1726719","article-title":"Impact of in-feed sodium butyrate or sodium heptanoate protected with medium-chain fatty acids on gut health in weaned piglets challenged with Escherichia coli F4","volume":"74","author":"Castillejos","year":"2020","journal-title":"Arch. Anim. Nutr."},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1186\/s13567-019-0725-0","article-title":"Lsr operon is associated with AI-2 transfer and pathogenicity in avian pathogenic Escherichia coli","volume":"50","author":"Zuo","year":"2019","journal-title":"Vet. Res."},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"1778","DOI":"10.1099\/mic.0.000567","article-title":"Chemotaxis to self-generated AI-2 promotes biofilm formation in Escherichia coli","volume":"163","author":"Jani","year":"2017","journal-title":"Microbiology"},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"1113","DOI":"10.1002\/jobm.202100344","article-title":"Autoinducer-2-mediated quorum-sensing system resists T4 phage infection in Escherichia coli","volume":"61","author":"Ding","year":"2021","journal-title":"J. Basic. Microbiol."},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/j.micpath.2016.08.033","article-title":"Autoinducer-2 of quorum sensing is involved in cell damage caused by avian pathogenic Escherichia coli","volume":"99","author":"Cui","year":"2016","journal-title":"Microb. Pathog."},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"669","DOI":"10.1096\/fasebj.2018.32.1_supplement.669.11","article-title":"Serotonin Promotes Enterohemorrhagic Escherichia Coli Pathogenesis Through Altered AI-2 Production by Gut Microbiota","volume":"32","author":"Menon","year":"2018","journal-title":"FASEB J."},{"key":"ref_100","doi-asserted-by":"crossref","unstructured":"Wits\u00f8, I.L., Valen Rukke, H., Benneche, T., and Aamdal Scheie, A. (2016). Thiophenone Attenuates Enteropathogenic Escherichia coli O103:H2 Virulence by Interfering with AI-2 Signaling. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0157334"},{"key":"ref_101","doi-asserted-by":"crossref","unstructured":"Helmy, Y.A., Kathayat, D., Deblais, L., Srivastava, V., Closs, G., Tokarski, R.J., Ayinde, O., Fuchs, J., and Rajashekara, G. (2022). Evaluation of Novel Quorum Sensing Inhibitors Targeting Auto-Inducer 2 (AI-2) for the Control of Avian Pathogenic Escherichia coli Infections in Chickens. Microbiol. Spectr., 10.","DOI":"10.1128\/spectrum.00286-22"},{"key":"ref_102","doi-asserted-by":"crossref","first-page":"560","DOI":"10.1016\/j.lwt.2015.11.013","article-title":"Inhibitory effects of lactic and malic organic acids on autoinducer type 2 (AI-2) quorum sensing of Escherichia coli O157:H7 and Salmonella Typhimurium","volume":"66","author":"Almasoud","year":"2016","journal-title":"LWT-Food Sci. Technol."},{"key":"ref_103","doi-asserted-by":"crossref","unstructured":"Escobar-Muci\u00f1o, E., Arenas-Hern\u00e1ndez, M.M.P., and Luna-Guevara, M.L. (2022). Mechanisms of Inhibition of Quorum Sensing as an Alternative for the Control of E. coli and Salmonella. Microorganisms, 10.","DOI":"10.3390\/microorganisms10050884"},{"key":"ref_104","doi-asserted-by":"crossref","unstructured":"Long, J., Guan, P., Hu, X., Yang, L., He, L., Lin, Q., Luo, F., Li, J., He, X., and Du, Z. (2021). Natural Polyphenols as Targeted Modulators in Colon Cancer: Molecular Mechanisms and Applications. Front. Immunol., 12.","DOI":"10.3389\/fimmu.2021.635484"},{"key":"ref_105","doi-asserted-by":"crossref","unstructured":"Yu, W., Sun, S., and Fu, Q. (2025). The role of short-chain fatty acid in metabolic syndrome and its complications: Focusing on immunity and inflammation. Front. Immunol., 16.","DOI":"10.3389\/fimmu.2025.1519925"},{"key":"ref_106","doi-asserted-by":"crossref","unstructured":"Khan, H., Ullah, H., Aschner, M., Cheang, W.S., and Akkol, E.K. (2019). Neuroprotective Effects of Quercetin in Alzheimer\u2019s Disease. Biomolecules, 10.","DOI":"10.3390\/biom10010059"},{"key":"ref_107","doi-asserted-by":"crossref","unstructured":"Revankar, A.A., Patil, A.S., Karishetti, R., Chougule, K.R., Patil, P., and Salokhe, A. (2025). Enhanced bioavailability of Quercetin-loaded niosomal in situ gel for the management of Parkinson\u2019s disease. Front. Pharmacol., 15.","DOI":"10.3389\/fphar.2024.1519649"},{"key":"ref_108","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1016\/j.bbadis.2012.11.018","article-title":"Quercetin supplementation is effective in improving mitochondrial dysfunctions induced by 3-nitropropionic acid: Implications in Huntington\u2019s disease","volume":"1832","author":"Sandhir","year":"2013","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_109","doi-asserted-by":"crossref","first-page":"114934","DOI":"10.1016\/j.bbr.2024.114934","article-title":"Quercetin attenuates brain apoptosis in mice with chronic unpredictable mild stress-induced depression","volume":"465","author":"Ge","year":"2024","journal-title":"Behav. Brain Res."},{"key":"ref_110","doi-asserted-by":"crossref","unstructured":"Deng, T.T., Ding, W.Y., Lu, X.X., Zhang, Q.H., Du, J.X., Wang, L.J., Yang, M.N., Yin, Y., and Liu, F.J. (2024). Pharmacological and mechanistic aspects of quercetin in osteoporosis. Front. Pharmacol., 15.","DOI":"10.3389\/fphar.2024.1338951"},{"key":"ref_111","doi-asserted-by":"crossref","first-page":"741","DOI":"10.2147\/IJN.S277377","article-title":"Effect of Quercetin-Loaded Mesoporous Silica Nanoparticles on Myocardial Ischemia-Reperfusion Injury in Rats and Its Mechanism","volume":"16","author":"Liu","year":"2021","journal-title":"Int. J. Nanomedicine."},{"key":"ref_112","doi-asserted-by":"crossref","first-page":"1700031","DOI":"10.1002\/mnfr.201700031","article-title":"Quercetin protects against atherosclerosis by inhibiting dendritic cell activation","volume":"61","author":"Lin","year":"2017","journal-title":"Mol. Nutr. Food Res."},{"key":"ref_113","doi-asserted-by":"crossref","first-page":"355","DOI":"10.2174\/0929867323666160909153707","article-title":"The Antidiabetic Potential of Quercetin: Underlying Mechanisms","volume":"24","author":"Eid","year":"2017","journal-title":"Curr. Med. Chem."},{"key":"ref_114","doi-asserted-by":"crossref","first-page":"5665778","DOI":"10.1155\/2022\/5665778","article-title":"Biological Activities Underlying the Therapeutic Effect of Quercetin on Inflammatory Bowel Disease","volume":"2022","author":"Lyu","year":"2022","journal-title":"Mediators Inflamm."},{"key":"ref_115","doi-asserted-by":"crossref","first-page":"5632260","DOI":"10.1155\/2024\/5632260","article-title":"Quercetin Increases Growth Performance and Decreases Incidence of Diarrhea and Mechanism of Action in Weaned Piglets","volume":"2024","author":"Mao","year":"2024","journal-title":"Oxid. Med. Cell. Longev."},{"key":"ref_116","doi-asserted-by":"crossref","first-page":"140262","DOI":"10.1016\/j.foodchem.2024.140262","article-title":"The bioavailability, absorption, metabolism, and regulation of glucolipid metabolism disorders by quercetin and its important glycosides: A review","volume":"458","author":"Zhu","year":"2024","journal-title":"Food Chem."}],"container-title":["Antioxidants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-3921\/14\/7\/852\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:08:39Z","timestamp":1760033319000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-3921\/14\/7\/852"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,11]]},"references-count":116,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2025,7]]}},"alternative-id":["antiox14070852"],"URL":"https:\/\/doi.org\/10.3390\/antiox14070852","relation":{},"ISSN":["2076-3921"],"issn-type":[{"value":"2076-3921","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,11]]}}}