{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:32:41Z","timestamp":1760059961132,"version":"build-2065373602"},"reference-count":38,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2025,7,24]],"date-time":"2025-07-24T00:00:00Z","timestamp":1753315200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund (FWF)","doi-asserted-by":"publisher","award":["10.55776\/P35129","244995","GZ 651.483\/0001-II\/2\/2010","GZ 651.484\/0001-II\/2\/2010"],"award-info":[{"award-number":["10.55776\/P35129","244995","GZ 651.483\/0001-II\/2\/2010","GZ 651.484\/0001-II\/2\/2010"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]},{"name":"European Union Framework Programme 7 FP7 2007-2013","award":["10.55776\/P35129","244995","GZ 651.483\/0001-II\/2\/2010","GZ 651.484\/0001-II\/2\/2010"],"award-info":[{"award-number":["10.55776\/P35129","244995","GZ 651.483\/0001-II\/2\/2010","GZ 651.484\/0001-II\/2\/2010"]}]},{"name":"Austrian Federal Ministry of Science, Research and Economy to the University of Graz","award":["10.55776\/P35129","244995","GZ 651.483\/0001-II\/2\/2010","GZ 651.484\/0001-II\/2\/2010"],"award-info":[{"award-number":["10.55776\/P35129","244995","GZ 651.483\/0001-II\/2\/2010","GZ 651.484\/0001-II\/2\/2010"]}]},{"name":"Medical University of Graz","award":["10.55776\/P35129","244995","GZ 651.483\/0001-II\/2\/2010","GZ 651.484\/0001-II\/2\/2010"],"award-info":[{"award-number":["10.55776\/P35129","244995","GZ 651.483\/0001-II\/2\/2010","GZ 651.484\/0001-II\/2\/2010"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>Oxidized phospholipids (OxPLs) are increasingly recognized as biologically active lipids involved in various pathologies. Both exposure to pathogenic factors and the efficacy of protective mechanisms are critical to disease development. In this study, we characterized an immunoassay that quantified the total capacity of the plasma to degrade or mask OxPLs, thereby preventing their interaction with cells and soluble proteins. OxLDL-coated plates were first incubated with human blood plasma or a control vehicle, followed by an ELISA using a monoclonal antibody specific to oxidized phosphatidylethanolamine. Pretreatment with the diluted blood plasma markedly inhibited mAb binding. The masking assay was optimized by evaluating the buffer composition, the compatibility with various anticoagulants, potential interfering compounds, the kinetic parameters, pre-analytical stability, statistical robustness, and intra- and inter-individual variability. We propose that this masking assay provides a simple immunological approach to assessing protective mechanisms against lipid peroxidation products. Establishing this robust and reproducible method is essential for conducting clinical association studies that explore masking activity as a potential biomarker of the predisposition to a broad range of lipid-peroxidation-related diseases.<\/jats:p>","DOI":"10.3390\/antiox14080903","type":"journal-article","created":{"date-parts":[[2025,7,24]],"date-time":"2025-07-24T07:54:26Z","timestamp":1753343666000},"page":"903","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["An Immune Assay to Quantify the Neutralization of Oxidation-Specific Epitopes by Human Blood Plasma"],"prefix":"10.3390","volume":"14","author":[{"given":"Marija","family":"Jelic","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Chemistry, Institute of Pharmaceutical Sciences, University of Graz, 8010 Graz, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-7768-0641","authenticated-orcid":false,"given":"Philipp","family":"Jokesch","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry, Institute of Pharmaceutical Sciences, University of Graz, 8010 Graz, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9443-8705","authenticated-orcid":false,"given":"Olga","family":"Oskolkova","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry, Institute of Pharmaceutical Sciences, University of Graz, 8010 Graz, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2184-0175","authenticated-orcid":false,"given":"Gernot","family":"Faustmann","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biosciences, University of Graz, 8010 Graz, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brigitte M.","family":"Winklhofer-Roob","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biosciences, University of Graz, 8010 Graz, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernd","family":"Ullrich","sequence":"additional","affiliation":[{"name":"Heidelberg ImmunoTherapeutics GmbH, 69115 Heidelberg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00fcrgen","family":"Krauss","sequence":"additional","affiliation":[{"name":"Vanudis GmbH, 69115 Heidelberg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rudolf","family":"\u00dcbelhart","sequence":"additional","affiliation":[{"name":"Vanudis GmbH, 69115 Heidelberg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4910-8322","authenticated-orcid":false,"given":"Bernd","family":"Gesslbauer","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry, Institute of Pharmaceutical Sciences, University of Graz, 8010 Graz, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3043-1736","authenticated-orcid":false,"given":"Valery","family":"Bochkov","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry, Institute of Pharmaceutical Sciences, University of Graz, 8010 Graz, Austria"},{"name":"Field of Excellence BioHealth, University of Graz, 8010 Graz, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1038\/nri.2016.63","article-title":"Innate sensing of oxidation-specific epitopes in health and disease","volume":"16","author":"Binder","year":"2016","journal-title":"Nat. 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