{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:33:30Z","timestamp":1760060010935,"version":"build-2065373602"},"reference-count":43,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2025,7,25]],"date-time":"2025-07-25T00:00:00Z","timestamp":1753401600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Universidade Estadual do Paran\u00e1"},{"name":"Programa de P\u00f3s-gradua\u00e7\u00e3o em Ci\u00eancias Aplicadas \u00e0 Sa\u00fade, PPGCAS"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>Scientific evidence has suggested, in most cases, that nullity of the GSTM1 and GSTT1 genes is associated with worse pathological outcomes and viral infections. In this sense, the main objective of this work was to determine the genotypic frequencies of GSTM1 and GSTT1 polymorphisms in individuals with HIV and to establish a possible relationship with CD4+ T lymphocyte count. This was a cross-sectional study, with a quantitative approach, composed of 182 HIV-positive patients. To detect GSTM1 and GSTT1 polymorphisms by the multiplex polymerase chain reaction (PCR), oral mucosa samples were collected. Regarding genotypic frequencies, GST nullity was high in the population, being 97.5% and 97.6%, respectively, for GSTM1\u2212 and GSTT1\u2212. Although there was no association between the GST polymorphism and the viral load and CD4+ T lymphocyte counts at diagnosis, when related to the current CD4+ count, the isolated and combined null alleles, GSTT1 (ORadj: 0.219; p = 0.004), GSTM1 (ORadj: 0.219; p = 0.004), and GSTM1\/T1 (ORadj: 0.219; p = 0.004), were defined as factors favorable to a minimum CD4+ T lymphocyte count of 350 cells. Therefore, this study demonstrated a probable relationship between the GSTT1 and GSTM1 genetic polymorphisms and HIV.<\/jats:p>","DOI":"10.3390\/antiox14080909","type":"journal-article","created":{"date-parts":[[2025,7,25]],"date-time":"2025-07-25T08:13:31Z","timestamp":1753431211000},"page":"909","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Nullity of GSTM1 and GSTT1 Associated with CD4+ T Cells in HIV-Positive Patients from Southern Brazil"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1703-7200","authenticated-orcid":false,"given":"Marcela Gon\u00e7alves","family":"Trevisan","sequence":"first","affiliation":[{"name":"Postgraduate Program in Applied Health Sciences, State University of West Paran\u00e1, Unioeste, Francisco Beltr\u00e3o 85.601-839, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcieli Borba do","family":"Nascimento","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Applied Health Sciences, State University of West Paran\u00e1, Unioeste, Francisco Beltr\u00e3o 85.601-839, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valdir","family":"Spada Jun\u00edor","sequence":"additional","affiliation":[{"name":"Undergraduate Course in Medicine, State University of West Paran\u00e1, Unioeste, Francisco Beltr\u00e3o 85.601-839, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Volmir Pitt","family":"Benedetti","sequence":"additional","affiliation":[{"name":"Paran\u00e1 University, Unipar, Francisco Beltr\u00e3o 85.601-000, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0757-3659","authenticated-orcid":false,"given":"Lirane Elize Defante","family":"Ferreto","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Applied Health Sciences, State University of West Paran\u00e1, Unioeste, Francisco Beltr\u00e3o 85.601-839, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L\u00e9ia Carolina","family":"Lucio","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Applied Health Sciences, State University of West Paran\u00e1, Unioeste, Francisco Beltr\u00e3o 85.601-839, Brazil"},{"name":"Laboratory of Molecular Biology and Human Genetics, State University of West Paran\u00e1, Unioeste, Francisco Beltr\u00e3o 85.601-839, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"50231","DOI":"10.34117\/bjdv8n7-101","article-title":"Main immunological alterations resulting from infection by the Acquired Human Immunodeficiency virus -HIV","volume":"8","author":"Almeida","year":"2022","journal-title":"Braz. J. Dev."},{"key":"ref_2","first-page":"281","article-title":"AIDS epidemiologic profile in Brazil using the Datasus database","volume":"52","author":"Oliveira","year":"2020","journal-title":"RBAC"},{"key":"ref_3","unstructured":"(2022, November 15). Brasil. Minist\u00e9rio da Sa\u00fade. Secretaria de Vigil\u00e2ncia em Sa\u00fade. Boletim Epidemiol\u00f3gico HIV\/AIDS. Bras\u00edlia 2021, Minist\u00e9rio da Sa\u00fade, Available online: https:\/\/www.gov.br\/saude\/pt-br\/centrais-de-conteudo\/publicacoes\/boletins\/epidemiologicos\/especiais\/2021\/boletim-epidemiologico-especial-hiv-aids-2021.pdf\/view."},{"key":"ref_4","unstructured":"Duarte Filho, E.C. (2022, November 15). Prevalence of oral lesions strongly associated with HIV in patients attended at the immunology outpatient clinic of Brazilian reference center for HIV\/AIDS infection. Dissertation, Universidade Federal do Rio de Janeiro. Available online: http:\/\/www.repositorio-bc.unirio.br:8080\/xmlui\/handle\/unirio\/12803."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Da Silva, D.G., Lima, R.C.C., de Oliveira, F.G., Otero, S.G., Nat\u00e1rio, R.M., Pereira, L.T.T., Paz, R.A.A., de Oliveira, C.F., de Oliveira Vinhort, G.F., and dos Santos, M.L.F. (2021). Epidemiological profile of HIV\/AIDS inpatients in Brazil: An integrative literature review. Res. Soc. Dev., 10.","DOI":"10.33448\/rsd-v10i9.17976"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.canlet.2018.06.028","article-title":"The multifaceted role of glutathione S-transferases in cancer","volume":"433","author":"Chatterjee","year":"2018","journal-title":"Cancer Lett."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"21885","DOI":"10.34117\/bjdv6n4-381","article-title":"The polymorphism of the gstm1 and gstt1genes in patients with microdelection in the azf region","volume":"6","author":"Lima","year":"2020","journal-title":"Braz. J. Dev."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"248","DOI":"10.5935\/0101-2800.20150038","article-title":"GSTM1 and GSTT1 genes null polymorphisms in kidney cancer susceptibility: Evidence based on a meta-analysis","volume":"37","author":"Silva","year":"2015","journal-title":"J. Bras. Nefrol."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Mondal, R., Ghosh, S.K., Choudhury, J.H., Seram, A., Sinha, K., Hussain, M., Laskar, R.S., Rabha, B., Dey, P., and Ganguli, S. (2013). Mitochondrial DNA copy number and risk of oral cancer: A report from Northeast India. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0057771"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"6356","DOI":"10.1073\/pnas.97.12.6356","article-title":"Molecular modeling and in vitro activity of an HIV-1-encoded glutathione peroxidase","volume":"97","author":"Zhao","year":"2000","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"4175","DOI":"10.1182\/blood.V89.11.4175","article-title":"Augmented intracellular glutathione inhibits Fas-triggered apoptosis of activated human neutrophils","volume":"89","author":"Watson","year":"1997","journal-title":"Blood"},{"key":"ref_12","first-page":"56","article-title":"Glutathione-S-transferase (M1 and T1) polymorphisms in Nigerian populations","volume":"3","author":"Benjamin","year":"2011","journal-title":"J. Med. Genet. Genom."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"13066","DOI":"10.4238\/2015.October.26.2","article-title":"Association of the GSTM1 null polymorphism with breast cancer in a Mexican population","volume":"14","author":"Figuera","year":"2015","journal-title":"Genet. Mol. Res."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"440","DOI":"10.1002\/(SICI)1098-2280(1997)30:4<440::AID-EM9>3.0.CO;2-M","article-title":"Glutathione S-transferase-mediated induction of GC\u2192AT transitions by halomethanes in salmonella","volume":"30","author":"DeMarini","year":"1997","journal-title":"Environ. Mol. Mutagen."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"6631","DOI":"10.1007\/s11033-021-06515-6","article-title":"Relationship between GSTM1 and GSTT1 polymorphisms and HPV infection: A systematic review","volume":"48","author":"Bortolli","year":"2021","journal-title":"Mol. Biol. Rep."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2251","DOI":"10.31557\/APJCP.2020.21.8.2251","article-title":"Frequency and association of GSTM1 and GSTT1 gene polymorphisms with survival in breast cancer patients","volume":"21","author":"Tangkheunkan","year":"2020","journal-title":"Asian Pac. J. Cancer Prev."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"259","DOI":"10.2478\/jomb-2013-0028","article-title":"Glutathione-S-transferase M1 and T1 gene polymorphisms and susceptibility to the progression of liver fibrosis in HCV-infected patients in Taiwan","volume":"33","author":"Sun","year":"2014","journal-title":"J. Med. Biochem."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Kuleape, J.A., Tagoe, E.A., Puplampu, P., Bonney, E.Y., and Quaye, O. (2018). Homozygous deletion of both GSTM1 and GSTT1 genes is associated with higher CD4+ T cell counts in Ghanaian HIV patients. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0195954"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1038\/tpj.2015.88","article-title":"Impact of GSTM1, GSTT1 and GSTP1 gene polymorphism and risk of ARV-associated hepatotoxicity in HIV-infected individuals and its modulation","volume":"17","author":"Singh","year":"2017","journal-title":"Pharmacogenomics J."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Chanhom, N., Jittikoon, J., Wattanapokayakit, S., Mahasirimongkol, S., Charoenyingwattana, A., Udomsinprasert, W., Chaikledkaew, U., Suvichapanich, S., Mushiroda, T., and Kiertiburanakul, S. (2022). The Association of HLA-B* 35 and GSTT1 Genotypes and Hepatotoxicity in Thai People Living with HIV. J. Pers. Med., 12.","DOI":"10.3390\/jpm12060940"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"6467","DOI":"10.1007\/s11033-022-07475-1","article-title":"GSTT1 and GSTM1 polymorphisms with human papillomavirus infection in women from southern Brazil: A case-control study","volume":"49","author":"Bortolli","year":"2022","journal-title":"Mol. Biol. Rep."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1590\/S0100-72032013000500007","article-title":"Risk factors and prevalence of HPV infection in patients from Basic Health Units of an University Hospital in southern Brazil","volume":"35","author":"Oliveira","year":"2013","journal-title":"Rev. Bras. Ginecol. Obs."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"e20200233","DOI":"10.1590\/1983-1447.2021.20200233","article-title":"Prevalence and factors associated to cervical changes in units from the Single Health System","volume":"42","author":"Treco","year":"2021","journal-title":"Rev. Ga\u00facha Enferm."},{"key":"ref_24","first-page":"9","article-title":"Detection of Human Papilomavirus (HPV) from buccal swabs","volume":"16","author":"Dingueleski","year":"2017","journal-title":"Rev. Gest\u00e3o Sa\u00fade"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1007\/s00430-018-0557-y","article-title":"Polymorphisms in the TGFB1 signal peptide influence human papillomavirus infection and development of cervical lesions","volume":"208","author":"Trugilo","year":"2019","journal-title":"Med. Microbiol. Immunol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1590\/1678-4685-gmb-2016-0142","article-title":"GSTT1 and GSTM1 null variants in Mestizo and Amerindian populations from northwestern Mexico and a literature review","volume":"40","author":"Orozco","year":"2017","journal-title":"Genet. Mol. Biol."},{"key":"ref_27","first-page":"10002337","article-title":"Effect of GSTM1-polymorphism on disease progression and oxidative stress in HIV infection: Modulation by HIV\/HCV co-infection and alcohol consumption","volume":"4","author":"Parsons","year":"2013","journal-title":"J. AIDS Clin. Res."},{"key":"ref_28","first-page":"41","article-title":"Role of glutathione S-transferase (GSTM1 and GSTT1) genes deletion in susceptibility to HIV-1 disease progression","volume":"8","author":"Djigma","year":"2020","journal-title":"J. Biosci. Med."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1007\/s11033-019-05143-5","article-title":"Genetic polymorphism of drug metabolism enzymes (GSTM1, GSTT1 and GSTP1) in the healthy Malian population","volume":"47","author":"Kassogue","year":"2020","journal-title":"Mol. Biol. Rep."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"49","DOI":"10.18316\/sdh.v6i1.3929","article-title":"Comparative Analysis Between Microsatellite Markers STR and Single Nucleotide Polymorphism SNP Used in The Forensic Area","volume":"6","author":"Machado","year":"2018","journal-title":"Rev. Sa\u00fade E Desenvolv. Hum."},{"key":"ref_31","unstructured":"Lopes, R.E. (2020). The Role of Single Nucleotide Polymorphisms (SNPs) of MiRNAs 100 and 146a as Prognostic Factors for Prostate Cancer. [Ph.D. Thesis, Universidade de S\u00e3o Paulo]."},{"key":"ref_32","unstructured":"Borges, P.V. (2021). Polimorfismos Gen\u00e9ticos de Nucleot\u00eddeo \u00danico Relacionados a Sintomas Depressivos em Pacientes Oncol\u00f3gicos: Uma Scoping Review. [Master\u2019s Thesis, Universidade de S\u00e3o Paulo]."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1007\/s11010-011-0817-2","article-title":"The association between GSTT1, M1, and P1 polymorphisms with coronary artery disease in Western Iran","volume":"354","author":"Nomani","year":"2011","journal-title":"Mol. Cell Biochem."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1590\/S0104-42302010000300013","article-title":"GSTM1 and GSTT1 genes analysis in head and neck cancer patients","volume":"56","author":"Leme","year":"2010","journal-title":"Rev. Assoc. Med. Bras."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"5446","DOI":"10.1002\/jmv.27076","article-title":"Association of GSTM1 and GSTT1 gene polymorphisms with COVID-19 susceptibility and its outcome","volume":"93","author":"Abbas","year":"2021","journal-title":"J. Med. Virol."},{"key":"ref_36","unstructured":"(2022, November 15). Brasil. Minist\u00e9rio da Sa\u00fade. Secretaria de Vigil\u00e2ncia em Sa\u00fade. Departamento de DST, Aids e Hepatites Virais. Manual T\u00e9cnico para o Diagn\u00f3stico da Infec\u00e7\u00e3o pelo HIV. Bras\u00edlia, Minist\u00e9rio da Sa\u00fade, Available online: https:\/\/bvsms.saude.gov.br\/bvs\/publicacoes\/manual_tecnico_diagnostico_infeccao_hiv.pdf."},{"key":"ref_37","first-page":"e-31101","article-title":"Effectiveness of antiretroviral treatment after 12 and 66 months at a reference centre for people living with HIV, Belo Horizonte, Minas Gerais; 2012-2018","volume":"31","author":"Raposo","year":"2021","journal-title":"Rev. Med. Minas. Gerais."},{"key":"ref_38","first-page":"84","article-title":"Polimorfismos HIV: Impactos na TARV\/HIV","volume":"13","author":"Lima","year":"2020","journal-title":"Braz. J. Surg. Clin. Res."},{"key":"ref_39","first-page":"33","article-title":"Avalia\u00e7\u00e3o de efic\u00e1cia da terapia antirretroviral em portadores de HIV\/AIDS atendidos em servi\u00e7o de refer\u00eancia do interior de S\u00e3o Paulo","volume":"22","author":"Pernomian","year":"2018","journal-title":"Braz. J. Infectar Dis."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1253","DOI":"10.1007\/s00228-017-2295-2","article-title":"Impact of glutathione transferases genes polymorphisms in nevirapine adverse reactions: A possible role for GSTM1 in SJS\/TEN susceptibility","volume":"73","author":"Ciccacci","year":"2017","journal-title":"Eur. J. Clin. Pharmacol."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Pagnotta, P.A., Melito, V.A., Lavandera, J.V., Parera, V.E., Rossetti, M.V., Zuccoli, J.R., and Buzaleh, A.M. (2021). Role of ABCB1 and glutathione S transferase gene variants in the association of porphyria cutanea tarda and human immunodeficiency virus infection. Biomed. Rep., 14.","DOI":"10.3892\/br.2020.1398"},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Ivanov, A.V., Valuev-Elliston, V.T., Ivanova, O.N., Kochetkov, S.N., Starodubova, E.S., Bartosch, B., and Isaguliants, M.G. (2016). Oxidative Stress during HIV Infection: Mechanisms and Consequences. Oxid Med. Cell. Longev., 8910396.","DOI":"10.1155\/2016\/8910396"},{"key":"ref_43","unstructured":"(2022, November 15). Brasil. Minist\u00e9rio da Sa\u00fade. Secretaria de Vigil\u00e2ncia em Sa\u00fade. Departamento de Vigil\u00e2ncia, Preven\u00e7\u00e3o e Controle das Infec\u00e7\u00f5es Sexualmente Transmiss\u00edveis, do HIV\/Aids e das Hepatites Virais. Protocolo Cl\u00ednico e Diretrizes Terap\u00eauticas para Manejo da Infec\u00e7\u00e3o pelo HIV em Adultos. Bras\u00edlia, Minist\u00e9rio da Sa\u00fade, Available online: https:\/\/www.gov.br\/aids\/pt-br\/centrais-de-conteudo\/pcdts\/2013\/hiv-aids\/pcdt_manejo_adulto_12_2018_web.pdf\/view."}],"container-title":["Antioxidants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-3921\/14\/8\/909\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:15:44Z","timestamp":1760033744000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-3921\/14\/8\/909"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,25]]},"references-count":43,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2025,8]]}},"alternative-id":["antiox14080909"],"URL":"https:\/\/doi.org\/10.3390\/antiox14080909","relation":{},"ISSN":["2076-3921"],"issn-type":[{"type":"electronic","value":"2076-3921"}],"subject":[],"published":{"date-parts":[[2025,7,25]]}}}