{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:32:54Z","timestamp":1760059974889,"version":"build-2065373602"},"reference-count":25,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2025,7,29]],"date-time":"2025-07-29T00:00:00Z","timestamp":1753747200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Florida Department of Health James and Esther King Biomedical Research Program","award":["20K10"],"award-info":[{"award-number":["20K10"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>Introduction: This study aimed to analyze exhaled breath condensate (EBC) for 8-oxoguanine (8-oxoGua), an oxidative stress biomarker among waterpipe (WP) smokers. Methods: In a within-subject pre-post exposure design, thirty waterpipe smokers completed two 45 min laboratory sessions. EBC was analyzed for 8-oxoGua before and after WP smoking. Median differences between time points (pre vs. post) were assessed using the Wilcoxon sign rank test, with significance defined as p &lt; 0.05. Results: The analysis included 59 WP smoking sessions. Participants had a median age of 24 years (IQR: 21\u201325), with 62.1% being female. Most had a bachelor\u2019s degree or less (62.1%), and over half were students (55.2%), while 34.5% were employed. The average age for first WP use was 18.6 years, with participants reporting a median of three WP smoking sessions per month. Results indicate a median increase in 8-oxoGua among participants from 5.4 ng\/mL (IQR: 8.8) before the smoking session to 7.6 ng\/mL after (IQR: 15.7; p &lt; 0.001). Conclusions: This study is the first to examine 8-oxoGua in EBC. Findings provide strong evidence of WP smoking\u2019s contribution to oxidative stress in the airways. It justifies the use of EBC to study the exposure to markers of oxidative stress with emerging tobacco use methods such as the waterpipe.<\/jats:p>","DOI":"10.3390\/antiox14080929","type":"journal-article","created":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T07:37:23Z","timestamp":1753861043000},"page":"929","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Analyzing 8-Oxoguanine in Exhaled Breath Condensate: A Novel Within-Subject Laboratory Experimental Study on Waterpipe Smokers"],"prefix":"10.3390","volume":"14","author":[{"given":"Natasha","family":"Shaukat","sequence":"first","affiliation":[{"name":"Department of Epidemiology, Robert Stempel College of Public Health, Florida International University, 11200 SW 8th St, Miami, FL 33199, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tarana","family":"Ferdous","sequence":"additional","affiliation":[{"name":"Department of Epidemiology, Robert Stempel College of Public Health, Florida International University, 11200 SW 8th St, Miami, FL 33199, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simanta","family":"Roy","sequence":"additional","affiliation":[{"name":"Department of Epidemiology, Robert Stempel College of Public Health, Florida International University, 11200 SW 8th St, Miami, FL 33199, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sharika","family":"Ferdous","sequence":"additional","affiliation":[{"name":"Department of Epidemiology, Robert Stempel College of Public Health, Florida International University, 11200 SW 8th St, Miami, FL 33199, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sreshtha","family":"Chowdhury","sequence":"additional","affiliation":[{"name":"Department of Epidemiology, Robert Stempel College of Public Health, Florida International University, 11200 SW 8th St, Miami, FL 33199, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leonardo","family":"Maya","sequence":"additional","affiliation":[{"name":"Forensic & Analytical Toxicology Facility, Global & Forensic Justice Center, Florida International University, Miami, FL 33199, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anthony Paul","family":"DeCaprio","sequence":"additional","affiliation":[{"name":"Forensic & Analytical Toxicology Facility, Global & Forensic Justice Center, Florida International University, Miami, FL 33199, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wasim","family":"Maziak","sequence":"additional","affiliation":[{"name":"Department of Epidemiology, Robert Stempel College of Public Health, Florida International University, 11200 SW 8th St, Miami, FL 33199, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taghrid","family":"Asfar","sequence":"additional","affiliation":[{"name":"Department of Public Health Sciences, Miller School of Medicine, University of Miami, Miami, FL 33136, USA"},{"name":"Sylvester Comprehensive Cancer Center, Miller School of Medicine, University of Miami, Miami, FL 33136, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e917","DOI":"10.1161\/CIR.0000000000000671","article-title":"Water Pipe (Hookah) Smoking and Cardiovascular Disease Risk: A Scientific Statement from the American Heart Association","volume":"139","author":"Bhatnagar","year":"2019","journal-title":"Circulation"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"107457","DOI":"10.1016\/j.addbeh.2022.107457","article-title":"Prevalence and Predictors of Waterpipe Smoking Cessation among Young Adults in the US: Findings from a Population-Based Study","volume":"135","author":"Gautam","year":"2022","journal-title":"Addict. 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