{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T20:15:52Z","timestamp":1790799352691,"version":"4.1.0"},"reference-count":44,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T00:00:00Z","timestamp":1753833600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Italian Ministry for Research and Education (MUR)","award":["ECS 00000038"],"award-info":[{"award-number":["ECS 00000038"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>Liquid storage is an important tool used to prolong fresh semen shelf-life while protecting spermatozoa from damage, conserving their overall functionality, and ensuring better fertility than frozen semen from sheep. The increased production of reactive oxygen species (ROS) during sperm storage leads to a decline in sperm quality, particularly with regard to sperm nuclear DNA damage and mitochondrial membrane potential (MMP). This study evaluated the effect of storing Sarda ram semen at 15 \u00b0C for 7 h on its redox status, motility, morphology, acrosome integrity, ATP content, mitochondrial potential membrane, and in vivo fertility after artificial insemination. Two different extenders were compared: a lab-made skimmed milk (SM)-based extender and a commercial extender (OviXcell\u00ae, IMV-Technologies, France). Lower ROS levels in the SM (p &lt; 0.001) indicated that its oxidative status was better maintained compared to the commercial extender (CE). Antioxidant defenses (total antioxidant capacity, TEAC; superoxide dismutase, SOD; total thiols) were higher in the SM (p &lt; 0.01) than in the CE. SM also had higher MMP (p &lt; 0.05), acrosome integrity (p &lt; 0.05), ATP content (p &lt; 0.01), and in vivo fertilizing capacity (p &lt; 0.05) compared to the CE, which indicated higher semen quality. In conclusion, the SM extender, while maintaining a better oxidative\/antioxidant balance, ensured higher semen quality after 7 h of storage at 15 \u00b0C in vitro compared to the CE.<\/jats:p>","DOI":"10.3390\/antiox14080932","type":"journal-article","created":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T07:37:23Z","timestamp":1753861043000},"page":"932","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Effect of Different Extenders on the Oxidative Status and Fertility of Sarda Ram Liquid Semen Stored at 15 \u00b0C"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9900-5976","authenticated-orcid":false,"given":"Valeria","family":"Pasciu","sequence":"first","affiliation":[{"name":"Department of Veterinary Medicine, University of Sassari, Via Vienna 2, 07100 Sassari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charbel","family":"Nassif","sequence":"additional","affiliation":[{"name":"Department of Agricultural Sciences, Unit of Animal Science, University of Sassari, Viale Italia 39\/A, 07100 Sassari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Dattena","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Agricultural Research Agency of Sardinia, 07100 Sassari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4901-9904","authenticated-orcid":false,"given":"Sara","family":"Succu","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, University of Sassari, Via Vienna 2, 07100 Sassari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2708-3833","authenticated-orcid":false,"given":"Francesca","family":"Sotgiu","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, University of Sassari, Via Vienna 2, 07100 Sassari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6297-1556","authenticated-orcid":false,"given":"Antonello","family":"Cannas","sequence":"additional","affiliation":[{"name":"Department of Agricultural Sciences, Unit of Animal Science, University of Sassari, Viale Italia 39\/A, 07100 Sassari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5049-5713","authenticated-orcid":false,"given":"Ignazio","family":"Cossu","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Agricultural Research Agency of Sardinia, 07100 Sassari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Baralla","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, University of Sassari, Via Vienna 2, 07100 Sassari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabrizio","family":"Chessa","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Agricultural Research Agency of Sardinia, 07100 Sassari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2500-8063","authenticated-orcid":false,"given":"Fiammetta","family":"Berlinguer","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, University of Sassari, Via Vienna 2, 07100 Sassari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4186-2400","authenticated-orcid":false,"given":"Laura","family":"Mara","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Agricultural Research Agency of Sardinia, 07100 Sassari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Sohail, T., Zhang, L., Wang, X., Jiang, C., Wang, J., Sun, X., and Li, Y. 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