{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T21:16:15Z","timestamp":1791321375779,"version":"4.3.1"},"reference-count":51,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2025,8,5]],"date-time":"2025-08-05T00:00:00Z","timestamp":1754352000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Key Research and Development Program of China","award":["2023YFD1300801"],"award-info":[{"award-number":["2023YFD1300801"]}]},{"name":"National Key Research and Development Program of China","award":["ASTIP-IAS-08"],"award-info":[{"award-number":["ASTIP-IAS-08"]}]},{"name":"Agricultural Science and Technology Innovation Program in the Chinese Academy of Agricultural Sciences","award":["2023YFD1300801"],"award-info":[{"award-number":["2023YFD1300801"]}]},{"name":"Agricultural Science and Technology Innovation Program in the Chinese Academy of Agricultural Sciences","award":["ASTIP-IAS-08"],"award-info":[{"award-number":["ASTIP-IAS-08"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>The deterioration of uterine calcium transport capacity induced by aging is a common problem for late-laying period hens, causing decline in eggshell quality. This study aimed to investigate the effects and possible regulatory mechanisms of dietary puerarin (PU) on calcium transport and eggshell quality in aged hens. Two hundred eighty-eight Hubbard Efficiency Plus broiler breeder hens (50-week-old) were randomly allocated to three dietary treatments containing 0, 40, or 200 mg\/kg puerarin (PU), with 8 replicates of 12 birds each, for an 8-week trial. The results demonstrated that dietary PU ameliorated the eggshell thickness and strength, which in turn reduced the broken egg rate (p &lt; 0.05). Histological analysis showed that PU improved uterus morphology and increased epithelium height in the uterus (p &lt; 0.05). Antioxidative capacity was significantly improved via upregulation of Nrf2, HO-1, and GPX1 mRNA expression in the uterus (p &lt; 0.05), along with enhanced total antioxidant capacity (T-AOC) and glutathione peroxidase (GSH-PX) activity, and decreased levels of the oxidative stress marker malondialdehyde (MDA) (p &lt; 0.05). Meanwhile, PU treatment reduced the apoptotic index of the uterus, followed by a significant decrease in expression of pro-apoptotic genes Caspase3 and BAX and the rate of BAX\/BCL-2. Additionally, calcium content in serum and uterus, as well as the activity of Ca2+-ATPase in the duodenum and uterus, were increased by dietary PU (p &lt; 0.05). The genes involved in calcium transport including ER\u03b1, KCNA1, CABP-28K, and OPN in the uterus were upregulated by PU supplementation (p &lt; 0.05). The 16S rRNA gene sequencing revealed that dietary PU supplementation could reverse the age-related decline in the relative abundance of Bacteroidota within the uterus (p &lt; 0.05). Overall, dietary PU can improve eggshell quality and calcium transport through enhanced antioxidative defenses and mitigation of age-related uterine degeneration.<\/jats:p>","DOI":"10.3390\/antiox14080960","type":"journal-article","created":{"date-parts":[[2025,8,7]],"date-time":"2025-08-07T10:58:26Z","timestamp":1754564306000},"page":"960","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Puerarin Enhances Eggshell Quality by Mitigating Uterine Senescence in Late-Phase Laying Breeder Hens"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-2196-7298","authenticated-orcid":false,"given":"Zhenwu","family":"Huang","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China"},{"name":"State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Haidian, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangju","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Haidian, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-3572-832X","authenticated-orcid":false,"given":"Mengjie","family":"Xu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Haidian, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanru","family":"Shi","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Haidian, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinghai","family":"Feng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Haidian, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minhong","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Haidian, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6158-1254","authenticated-orcid":false,"given":"Chunmei","family":"Li","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"609","DOI":"10.5713\/ajas.2012.12398","article-title":"Microarray analysis of genes involved with shell strength in layer shell gland at the early stage of active calcification","volume":"26","author":"Liu","year":"2013","journal-title":"Asian-Australas. J. Anim. Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/j.theriogenology.2021.11.019","article-title":"Dietary mulberry-leaf flavonoids improve the eggshell quality of aged breeder hens","volume":"179","author":"Huang","year":"2022","journal-title":"Theriogenology"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2982","DOI":"10.1007\/s12011-022-03388-z","article-title":"Effects of Zinc, Manganese, and Taurine on Egg Shell Microstructure in Commercial Laying Hens After Peak Production","volume":"201","author":"Hajjarmanesh","year":"2023","journal-title":"Biol. Trace Elem. Res."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Feng, J., Zhang, H.J., Wu, S.G., Qi, G.H., and Wang, J. (2020). Uterine transcriptome analysis reveals mRNA expression changes associated with the ultrastructure differences of eggshell in young and aged laying hens. BMC Genom., 21.","DOI":"10.1186\/s12864-020-07177-7"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"16853","DOI":"10.1038\/s41598-018-35203-y","article-title":"RNA sequencing-based analysis of the laying hen uterus revealed the novel genes and biological pathways involved in the eggshell biomineralization","volume":"8","author":"Sah","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Wang, B., Wang, S., Ding, M., Lu, H., Wu, H., and Li, Y. (2021). Quercetin Regulates Calcium and Phosphorus Metabolism Through the Wnt Signaling Pathway in Broilers. Front. Vet. Sci., 8.","DOI":"10.3389\/fvets.2021.786519"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"895","DOI":"10.1093\/ps\/83.6.895","article-title":"Exogenous estrogen boosts circulating estradiol concentrations and calcium uptake by duodenal tissue in heat-stressed hens","volume":"83","author":"Hansen","year":"2004","journal-title":"Poult. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1016\/j.cbpa.2012.04.016","article-title":"Sodium deoxycholate inhibits chick duodenal calcium absorption through oxidative stress and apoptosis","volume":"162","author":"Rivoira","year":"2012","journal-title":"Comp. Biochem. Physiol. Part A Mol. Integr. Physiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1007\/BF00315999","article-title":"Immunohistochemical localization of a calcium pump and calbindin-D28k in the oviduct of the laying hen","volume":"96","author":"Wasserman","year":"1991","journal-title":"Histochemistry"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Surai, P.F., Kochish, I.I., and Kidd, M.T. (2021). Redox Homeostasis in Poultry: Regulatory Roles of NF-\u03baB. Antioxidants, 10.","DOI":"10.3390\/antiox10020186"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2685310","DOI":"10.1155\/2020\/2685310","article-title":"Oxidized Oils and Oxidized Proteins Induce Apoptosis in Granulosa Cells by Increasing Oxidative Stress in Ovaries of Laying Hens","volume":"2020","author":"Zhou","year":"2020","journal-title":"Oxidative Med. Cell. Longev."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"104003","DOI":"10.1016\/j.psj.2024.104003","article-title":"Oxidative stress in poultry production","volume":"103","author":"Oke","year":"2024","journal-title":"Poult. Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"2841","DOI":"10.3748\/wjg.v23.i16.2841","article-title":"Oxidative stress, antioxidants and intestinal calcium absorption","volume":"23","author":"Guizzardi","year":"2017","journal-title":"World J. Gastroenterol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"skaa025","DOI":"10.1093\/jas\/skaa025","article-title":"Dietary cadmium chloride impairs shell biomineralization by disrupting the metabolism of the eggshell gland in laying hens","volume":"98","author":"Zhu","year":"2020","journal-title":"J. Anim. Sci."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Dai, D., Wang, J., Zhang, H., Wu, S., and Qi, G. (2023). Uterine microbial communities and their potential role in the regulation of epithelium cell cycle and apoptosis in aged hens. Microbiome, 11.","DOI":"10.1186\/s40168-023-01707-7"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"104849","DOI":"10.1016\/j.psj.2025.104849","article-title":"Probiotics improve eggshell quality via regulating microbial composition in the uterine and cecum","volume":"104","author":"Lu","year":"2025","journal-title":"Poult. Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"114682","DOI":"10.1016\/j.anifeedsci.2020.114682","article-title":"Dietary stevioside supplementation improves laying performance and eggshell quality through increasing estrogen synthesis, calcium level and antioxidant capacity of reproductive organs in aged breeder hens-ScienceDirect","volume":"269","author":"Jiang","year":"2020","journal-title":"Anim. Feed. Sci. Technol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1016\/j.aninu.2024.04.007","article-title":"Dietary supplementation with calcitriol or quercetin improved eggshell and bone quality by modulating calcium metabolism","volume":"18","author":"Fu","year":"2024","journal-title":"Anim. Nutr."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"7022","DOI":"10.3382\/ps\/pez367","article-title":"Genistein improves the reproductive performance and bone status of breeder hens during the late egg-laying period","volume":"98","author":"Lv","year":"2019","journal-title":"Poult. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"110734","DOI":"10.1016\/j.biopha.2020.110734","article-title":"A comprehensive review on Pueraria: Insights on its chemistry and medicinal value","volume":"131","author":"Wang","year":"2020","journal-title":"Biomed. Pharmacother."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.lfs.2016.09.024","article-title":"Coadministration of puerarin (low dose) and zinc attenuates bone loss and suppresses bone marrow adiposity in ovariectomized rats","volume":"166","author":"Liu","year":"2016","journal-title":"Life Sci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"103420","DOI":"10.1016\/j.psj.2023.103420","article-title":"Effects of puerarin as a feed additive on the laying performance, egg quality, endocrine hormones, antioxidant capacity, and intestinal morphology of aged laying hens","volume":"103","author":"Du","year":"2024","journal-title":"Poult. Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"e2200663","DOI":"10.1002\/mnfr.202200663","article-title":"Puerarin Alleviates Oxidized Oil-Induced Oxidative Injury and Inflammation via Inhibition of the Nrf2\/Keap1 and HMGB1\/TLR4\/MAPK Signaling Pathways: An Investigation in a Chicken Model","volume":"67","author":"Zhou","year":"2023","journal-title":"Mol. Nutr. Food Res."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Lu, Y., Ge, S., Zhang, H., Lu, W., Bao, X., Pan, S., and Pang, Q. (2023). Metabolomic and microbiome analysis of the protective effects of Puerarin against Salmonella Enteritidis Infection in chicks. BMC Vet. Res., 19.","DOI":"10.1186\/s12917-023-03806-x"},{"key":"ref_25","unstructured":"(2004). China National Standard, Feeding Standard of Chicken (Standard No. NY\/T 33-2004)."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1016\/j.aninu.2022.06.002","article-title":"Maternal stevioside supplementation improves intestinal immune function of chicken offspring potentially via modulating gut microbiota and down-regulating the promoter methylation level of suppressor of cytokine signaling 1 (SOCS1)","volume":"10","author":"Jiang","year":"2022","journal-title":"Anim. Nutr."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"101622","DOI":"10.1016\/j.psj.2021.101622","article-title":"Eggshell decalcification and skeletal mineralization during chicken embryonic development: Defining candidate genes in the chorioallantoic membrane","volume":"101","author":"Halgrain","year":"2022","journal-title":"Poult. Sci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.aninu.2024.04.026","article-title":"Methionine supplementation regulates eggshell quality and uterine transcriptome in late-stage broiler breeders","volume":"19","author":"Gao","year":"2024","journal-title":"Anim. Nutr."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1477","DOI":"10.5713\/ajas.15.0655","article-title":"Effects of Dietary Calcium Levels on Productive Performance, Eggshell Quality and Overall Calcium Status in Aged Laying Hens","volume":"29","author":"An","year":"2016","journal-title":"Asian-Australas. J. Anim. Sci."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Zhang, J., Wang, Y., Zhang, C., Xiong, M., Rajput, S.A., Liu, Y., and Qi, D. (2019). The differences of gonadal hormones and uterine transcriptome during shell calcification of hens laying hard or weak-shelled eggs. BMC Genom., 20.","DOI":"10.1186\/s12864-019-6017-2"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"901","DOI":"10.1002\/jcb.10696","article-title":"Effect of growth and maturation on membrane-initiated actions of 1,25-dihydroxyvitamin D3-II: Calcium transport, receptor kinetics, and signal transduction in intestine of female chickens","volume":"90","author":"Larsson","year":"2003","journal-title":"J. Cell. Biochem."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"111","DOI":"10.3109\/13880209.2015.1017885","article-title":"Anti-osteoporotic activity of puerarin 6\u2033-O-xyloside on ovariectomized mice and its potential mechanism","volume":"54","author":"Li","year":"2016","journal-title":"Pharm. Biol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1016\/j.cbpa.2008.11.020","article-title":"Calcium transport in strongly calcifying laying birds: Mechanisms and regulation","volume":"152","author":"Bar","year":"2009","journal-title":"Comp. Biochem. Physiol. Part A Mol. Integr. Physiol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"101098","DOI":"10.1016\/j.psj.2021.101098","article-title":"Prolonged scotophase within a 24 hour light regime improves eggshell quality by enhancing calcium deposition in laying hens","volume":"100","author":"Xin","year":"2021","journal-title":"Poult. Sci."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Cheng, X., Li, X., Liu, Y., Ma, Y., Zhang, R., Zhang, Y., Fan, C., Qu, L., and Ning, Z. (2023). DNA methylome and transcriptome identified Key genes and pathways involved in Speckled Eggshell formation in aged laying hens. BMC Genom., 24.","DOI":"10.1186\/s12864-022-09100-8"},{"key":"ref_36","first-page":"787","article-title":"Puerarin stimulates proliferation and differentiation and protects against cell death in human osteoblastic MG-63 cells via ER-dependent MEK\/ERK and PI3K\/Akt activation","volume":"20","author":"Wang","year":"2013","journal-title":"Phytomedicine Int. J. Phytother. Phytopharm."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"eaar3219","DOI":"10.1126\/sciadv.aar3219","article-title":"Nanostructure, osteopontin, and mechanical properties of calcitic avian eggshell","volume":"4","author":"Athanasiadou","year":"2018","journal-title":"Sci. Adv."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"2606","DOI":"10.1089\/ars.2013.5406","article-title":"Ubiquinol-10 supplementation activates mitochondria functions to decelerate senescence in senescence-accelerated mice","volume":"20","author":"Tian","year":"2014","journal-title":"Antioxid. Redox Signal."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1321","DOI":"10.1111\/jpn.13658","article-title":"Dietary mulberry-leaf flavonoids supplementation improves liver lipid metabolism and ovarian function of aged breeder hens","volume":"106","author":"Huang","year":"2022","journal-title":"J. Anim. Physiol. Anim. Nutr."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"2644205","DOI":"10.1155\/2022\/2644205","article-title":"Intrauterine Growth Retardation Affects Intestinal Health of Suckling Piglets via Altering Intestinal Antioxidant Capacity, Glucose Uptake, Tight Junction, and Immune Responses","volume":"2022","author":"Tang","year":"2022","journal-title":"Oxidative Med. Cell. Longev."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Zhao, W., Cui, X., Wang, Z.Q., Yao, R., Chen, M.D., Gao, B.Y., Zhang, C.W., and Niu, J. (2022). Effects of Barranca yajiagengensis Powder in the Diet of Trachinotus ovatus on the Growth Performance, Antioxidant Capacity, Immunity and Morphology of the Liver and Intestine. Antioxidants, 11.","DOI":"10.3390\/antiox11071220"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"103281","DOI":"10.1016\/j.psj.2023.103281","article-title":"Protective effects of puerarin on liver tissue in Salmonella-infected chicks: A proteomic analysis","volume":"103","author":"Lu","year":"2024","journal-title":"Poult. Sci."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1146\/annurev-pharmtox-011112-140320","article-title":"Role of nrf2 in oxidative stress and toxicity","volume":"53","author":"Ma","year":"2013","journal-title":"Annu. Rev. Pharmacol. Toxicol."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Zhang, Q., Yao, M., Qi, J., Song, R., Wang, L., Li, J., Zhou, X., Chang, D., Huang, Q., and Li, L. (2023). Puerarin inhibited oxidative stress and alleviated cerebral ischemia-reperfusion injury through PI3K\/Akt\/Nrf2 signaling pathway. Front. Pharmacol., 14.","DOI":"10.3389\/fphar.2023.1134380"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"6604","DOI":"10.1002\/fsn3.3609","article-title":"Puerarin protects against acetaminophen-induced oxidative damage in liver through activation of the Keap1\/Nrf2 signaling pathway","volume":"11","author":"Zhou","year":"2023","journal-title":"Food Sci. Nutr."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1111\/j.1745-7270.2005.00108.x","article-title":"Caspase family proteases and apoptosis","volume":"37","author":"Fan","year":"2005","journal-title":"Acta Biochim. Biophys. Sin."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Liu, Y., Ye, F., Zou, W.J., Sun, Y., Wang, R., Han, P.P., Zhang, Z., Yang, X.L., and Liu, X. (2015). Baicalein reduces the occurrence of cirrhotic endotoxemia by reducing intestinal mucosal apoptosis. BMC Complement. Altern. Med., 15.","DOI":"10.1186\/s12906-015-0682-8"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"273","DOI":"10.3945\/jn.113.183731","article-title":"The undernourished neonatal mouse metabolome reveals evidence of liver and biliary dysfunction, inflammation, and oxidative stress","volume":"144","author":"Preidis","year":"2014","journal-title":"J. Nutr."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Rosell-Cardona, C., Amat, C., Gri\u00f1\u00e1n-Ferr\u00e9, C., Polo, J., Pall\u00e0s, M., P\u00e9rez-Bosque, A., Moret\u00f3, M., and Mir\u00f3, L. (2022). The Neuroprotective Effects of Spray-Dried Porcine Plasma Supplementation Involve the Microbiota-Gut-Brain Axis. Nutrients, 14.","DOI":"10.3390\/nu14112211"},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Shterzer, N., Rothschild, N., Sbehat, Y., Stern, E., Nazarov, A., and Mills, E. (2020). Large Overlap Between the Intestinal and Reproductive Tract Microbiomes of Chickens. Front. Microbiol., 11.","DOI":"10.3389\/fmicb.2020.01508"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"40248","DOI":"10.1038\/srep40248","article-title":"A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic","volume":"7","author":"Patrascu","year":"2017","journal-title":"Sci. Rep."}],"container-title":["Antioxidants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-3921\/14\/8\/960\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:23:14Z","timestamp":1760034194000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-3921\/14\/8\/960"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,5]]},"references-count":51,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2025,8]]}},"alternative-id":["antiox14080960"],"URL":"https:\/\/doi.org\/10.3390\/antiox14080960","relation":{},"ISSN":["2076-3921"],"issn-type":[{"value":"2076-3921","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8,5]]}}}