{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T05:42:45Z","timestamp":1771306965140,"version":"3.50.1"},"reference-count":80,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2025,8,21]],"date-time":"2025-08-21T00:00:00Z","timestamp":1755734400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"ANID\/FONDECYT\/1250170"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>This study investigates the phenolic composition and antioxidant potential of root extracts from three Opuntia ficus-indica varieties (green, red, and orange) using ultra-high-performance liquid chromatography coupled with diode array detection and electrospray ionization\u2013tandem mass spectrometry (UHPLC-DAD-ESI-MS\/MS). Phenolic compounds were extracted with a hydromethanolic solvent and quantified by spectrophotometric assays, while antioxidant activity was assessed through DPPH, ABTS, iron III reduction, hydroxyl radical, and nitric oxide scavenging methods. A total of 26 compounds were identified, including piscidic acid, epicatechin-3-O-gallate, and isovitexin, with several phenolics newly reported for O. ficus-indica roots. The green and red varieties showed the highest phenolic contents (up to 147.82 mg\/g extract) and strong antioxidant capacity, particularly in ABTS (IC50 = 29.38 \u03bcg\/mL) and hydroxyl radical inhibition (&gt;90%). Relative Antioxidant Capacity Index (RACI) analysis confirmed a consistent correlation between phenolic\/flavonoid content and antioxidant efficacy. These findings highlight the analytical relevance of UHPLC-DAD-ESI-MS\/MS for profiling underutilized plant matrices and support the potential use of O. ficus-indica root extracts as natural sources of bioactive compounds for pharmaceutical and biomedical applications.<\/jats:p>","DOI":"10.3390\/antiox14081023","type":"journal-article","created":{"date-parts":[[2025,8,21]],"date-time":"2025-08-21T08:02:44Z","timestamp":1755763364000},"page":"1023","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Identification, Quantification, and Antioxidant Evaluation of Phenolic Compounds from Colored Opuntia ficus-indica (L.) Roots Using UHPLC-DAD-ESI-MS\/MS"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2597-2549","authenticated-orcid":false,"given":"Elias","family":"Benramdane","sequence":"first","affiliation":[{"name":"Laboratoire de Biochimie Appliqu\u00e9e, Facult\u00e9 des Sciences de la Nature et de la Vie, Universit\u00e9 de Bejaia, Bejaia 06000, Algeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7254-5665","authenticated-orcid":false,"given":"Ahmad","family":"Mustafa","sequence":"additional","affiliation":[{"name":"Faculty of Engineering, October University for Modern Sciences and Arts (MSA), Giza 12451, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nadia","family":"Chougui","sequence":"additional","affiliation":[{"name":"D\u00e9partement des Sciences Alimentaires, Facult\u00e9 des Sciences de la Nature et de la Vie, Universit\u00e9 de Bejaia, Bejaia 06000, Algeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nawal","family":"Makhloufi","sequence":"additional","affiliation":[{"name":"Laboratoire de Biochimie Appliqu\u00e9e, Facult\u00e9 des Sciences de la Nature et de la Vie, Universit\u00e9 de Bejaia, Bejaia 06000, Algeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abderezak","family":"Tamendjari","sequence":"additional","affiliation":[{"name":"Laboratoire de Biochimie Appliqu\u00e9e, Facult\u00e9 des Sciences de la Nature et de la Vie, Universit\u00e9 de Bejaia, Bejaia 06000, Algeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cassamo U.","family":"Mussagy","sequence":"additional","affiliation":[{"name":"Laboratorio de Desarrollo de Bioprocesos Sostenibles (Labisost), Escuela de Agronom\u00eda, Facultad de Ciencias Agron\u00f3micas y de los Alimentos, Pontificia Universidad Cat\u00f3lica de Valpara\u00edso, Casilla 4-D, Quillota 2260000, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"105236","DOI":"10.1016\/j.resconrec.2020.105236","article-title":"Critical Success and Risk Factors for Circular Business Models Valorising Agricultural Waste and By-Products","volume":"165","author":"Donner","year":"2021","journal-title":"Resour. 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