{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T18:38:18Z","timestamp":1790793498157,"version":"4.1.0"},"reference-count":96,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2025,9,13]],"date-time":"2025-09-13T00:00:00Z","timestamp":1757721600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Research Foundation of Korea (NRF)","award":["RS-2023-00240204"],"award-info":[{"award-number":["RS-2023-00240204"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>The growing challenge of antibiotic resistance and inflammation-related disorders calls for safe, multi-target therapeutic strategies. Morus alba (MOAL) and Pinus densiflora (PIDE) are known for their medicinal properties, yet their combined potential with methyl gallate (MG) has not been fully explored. In this study, the phytochemical composition of MOAL and PIDE was characterized using GC\u2013MS, and their combined antimicrobial, antioxidant, and anti-inflammatory activities were evaluated. Hydroethanolic extracts were prepared and assessed for antioxidant activity (DPPH assay), antibacterial activity (disk diffusion, MIC, time kill), and nitric oxide (NO) suppression in Lipopolysaccharide (LPS)-stimulated macrophages, alongside MTT cytotoxicity screening. MOAL exhibited a higher extraction efficiency, reaching 500 mg\/mL at 4 h, whereas Pinus achieved 450 mg\/mL at the same time point. Both exhibited a diverse and abundant phytochemical profile. The optimized blend (MOAL:PIDE:MG, 1:1:0.1) demonstrated significantly enhanced bioactivity, with over 90% DPPH scavenging with the low IC50 value (66.62 mg\/mL), potent inhibition of both Gram-positive and Gram-negative bacteria, and the strongest effect against Staphylococcus aureus (264 \u03bcg\/mL). Time-kill assays confirmed rapid bactericidal action, and NO production was reduced by approximately 75% without cytotoxicity. Molecular docking identified a lead multi-target compound exhibiting strong binding affinities to COX-2, TNF-\u03b1, and Keap1, supporting its observed anti-inflammatory and antioxidant potential. These findings highlight the promise of synergistic phytochemical formulations as broad-spectrum, multifunctional therapeutic candidates, supporting further in vivo and clinical validation.<\/jats:p>","DOI":"10.3390\/antiox14091114","type":"journal-article","created":{"date-parts":[[2025,9,15]],"date-time":"2025-09-15T07:56:51Z","timestamp":1757923011000},"page":"1114","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Integrated Phytochemical Profiling, GC-MS Characterization, and In Silico, In Vitro Evaluation of Synergistic Antimicrobial, Antioxidant, and Anti-Inflammatory Activities of Morus alba Bark and Pinus densiflora Extracts with Methyl Gallate"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1071-4176","authenticated-orcid":false,"given":"Muhammad Aleem","family":"Abbas","sequence":"first","affiliation":[{"name":"Laboratory of Veterinary Pharmacokinetics, Institute for Veterinary Biomedical Science, College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-4088-2314","authenticated-orcid":false,"given":"Ga-Yeong","family":"Lee","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Pharmacokinetics, Institute for Veterinary Biomedical Science, College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Syed Al Jawad","family":"Sayem","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Pharmacokinetics, Institute for Veterinary Biomedical Science, College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7109-6423","authenticated-orcid":false,"given":"Seung-Jin","family":"Lee","sequence":"additional","affiliation":[{"name":"Developmental and Reproductive Toxicology Research Group, Korea Institute of Toxicology, Daejeon 34114, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8539-1087","authenticated-orcid":false,"given":"Seung-Chun","family":"Park","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Pharmacokinetics, Institute for Veterinary Biomedical Science, College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Republic of Korea"},{"name":"Cardiovascular Research Institute, Kyungpook National University, Daegu 41566, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,9,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Catalano, A., Iacopetta, D., Ceramella, J., Scumaci, D., Giuzio, F., Saturnino, C., Aquaro, S., Rosano, C., and Sinicropi, M.S. (2022). Multidrug resistance (MDR): A widespread phenomenon in pharmacological therapies. Molecules, 27.","DOI":"10.3390\/molecules27030616"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Ahmed, I., and Mikail, M. (2017). Paradigm shift: Focusing on plant-based natural antimicrobials. J. Microbiol. Exp., 5.","DOI":"10.15406\/jmen.2017.05.00145"},{"key":"ref_3","first-page":"452","article-title":"Antioxidant and antimicrobial activities of plants grown in the Mediterranean region","volume":"2","author":"Bayraktar","year":"2022","journal-title":"JSFA Rep."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"268","DOI":"10.20307\/nps.2019.25.3.268","article-title":"Phytochemical constituents of the root bark from Morus alba and their Il-6 inhibitory activity","volume":"25","author":"Chang","year":"2019","journal-title":"Nat. Prod. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"\u0160kovranov\u00e1, G., \u010culenov\u00e1, M., Treml, J., Dzurick\u00e1, L., Marova, I., and Sychrov\u00e1, A. (2022). Prenylated phenolics from Morus alba against MRSA infections as a strategy for wound healing. Front. Pharmacol., 13.","DOI":"10.3389\/fphar.2022.1068371"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"84","DOI":"10.3109\/08923973.2011.580755","article-title":"Sanggenon C and O inhibit NO production, iNOS expression and NF-\u03baB activation in LPS-induced RAW264. 7 cells","volume":"34","author":"Dat","year":"2012","journal-title":"Immunopharmacol. Immunotoxicol."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Park, S., Moon, B.R., Kim, J.E., Kim, H.J., and Zhang, T. (2020). Aqueous extracts of Morus alba root bark and cornus officinalis fruit protect against osteoarthritis symptoms in testosterone-deficient and osteoarthritis-induced rats. Pharmaceutics, 12.","DOI":"10.3390\/pharmaceutics12121245"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Oh, Y.J., Kim, Y.-S., Kim, J.W., and Kim, D.W. (2023). Antibacterial and antiviral properties of Pinus densiflora essential oil. Foods, 12.","DOI":"10.3390\/foods12234279"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Lee, J., Kang, H.K., Cheong, H., and Park, Y. (2021). A novel antimicrobial peptides from pine needles of Pinus densiflora Sieb. et Zucc. against foodborne bacteria. Front. Microbiol., 12.","DOI":"10.3389\/fmicb.2021.662462"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Zhang, Y., Chung, W.-K., Moon, S.-H., Lee, J.-G., and Om, A.-S. (2023). Comparison of Antibacterial Activities of Korean Pine (Pinus densiflora) Needle Steam Distillation Extract on Escherichia coli and Staphylococcus aureus Focusing on Membrane Fluidity and Genes Involved in Membrane Lipids and Stress. Molecules, 29.","DOI":"10.3390\/molecules29010165"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1148","DOI":"10.1080\/13880209.2022.2079679","article-title":"Extract of Pinus densiflora needles suppresses acute inflammation by regulating inflammatory mediators in RAW264. 7 macrophages and mice","volume":"60","author":"Jeong","year":"2022","journal-title":"Pharm. Biol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1186\/s40199-017-0184-y","article-title":"Pinus densiflora needle supercritical fluid extract suppresses the expression of pro-inflammatory mediators iNOS, IL-6 and IL-1\u03b2, and activation of inflammatory STAT1 and STAT3 signaling proteins in bacterial lipopolysaccharide-challenged murine macrophages","volume":"25","author":"Venkatesan","year":"2017","journal-title":"DARU J. Pharm. Sci."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Jiamboonsri, P., Eurtivong, C., and Wanwong, S. (2023). Assessing the potential of gallic acid and methyl gallate to enhance the efficacy of \u03b2-lactam antibiotics against methicillin-resistant Staphylococcus aureus by targeting \u03b2-lactamase: In silico and in vitro studies. Antibiotics, 12.","DOI":"10.3390\/antibiotics12111622"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"106849","DOI":"10.1016\/j.phrs.2023.106849","article-title":"Methyl gallate: Review of pharmacological activity","volume":"194","author":"Liang","year":"2023","journal-title":"Pharmacol. Res."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.phymed.2017.12.023","article-title":"Synergism of prenylflavonoids from Morus alba root bark against clinical MRSA isolates","volume":"39","author":"Zuo","year":"2018","journal-title":"Phytomedicine"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.jnutbio.2019.03.009","article-title":"Synergistic anti-inflammatory effects and mechanisms of combined phytochemicals","volume":"69","author":"Zhang","year":"2019","journal-title":"J. Nutr. Biochem."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Atchan, A.P.N., Monthe, O.C., Tchamgoue, A.D., Singh, Y., Shivashankara, S.T., Selvi, M.K., Agbor, G.A., Magni, P., Piazza, S., and Manjappara, U.V. (2023). Anti-inflammatory, antioxidant activities, and phytochemical characterization of edible plants exerting synergistic effects in human gastric epithelial cells. Antioxidants, 12.","DOI":"10.3390\/antiox12030591"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Boby, N., Abbas, M.A., Lee, E.-B., Im, Z.-E., Hsu, W.H., and Park, S.-C. (2021). Protective effect of Pyrus ussuriensis Maxim. extract against ethanol-induced gastritis in rats. Antioxidants, 10.","DOI":"10.3390\/antiox10030439"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Abbas, M.A., Boby, N., Lee, E.-B., Hong, J.-H., and Park, S.-C. (2022). Anti-obesity effects of Ecklonia cava extract in high-fat diet-induced obese rats. Antioxidants, 11.","DOI":"10.3390\/antiox11020310"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Hossain, M.A., Park, J.-Y., Kim, J.-Y., Suh, J.-W., and Park, S.-C. (2014). Synergistic effect and antiquorum sensing activity of Nymphaea tetragona (water lily) extract. BioMed Res. Int., 2014.","DOI":"10.1155\/2014\/562173"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Abbas, M.A., Lee, E.-B., Boby, N., Biruhanu, B.T., and Park, S.-C. (2022). A pharmacodynamic investigation to assess the synergism of orbifloxacin and propyl gallate against Escherichia coli. Front. Pharmacol., 13.","DOI":"10.3389\/fphar.2022.989395"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Quah, Y., Lee, S.-J., Lee, E.-B., Birhanu, B.T., Ali, M.S., Abbas, M.A., Boby, N., Im, Z.-E., and Park, S.-C. (2020). Cornus officinalis ethanolic extract with potential anti-allergic, anti-inflammatory, and antioxidant activities. Nutrients, 12.","DOI":"10.3390\/nu12113317"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1226","DOI":"10.1021\/acs.biochem.5b01378","article-title":"Crystal structure of aspirin-acetylated human cyclooxygenase-2: Insight into the formation of products with reversed stereochemistry","volume":"55","author":"Lucido","year":"2016","journal-title":"Biochemistry"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1186\/1742-6405-3-8","article-title":"LMP-420, a small-molecule inhibitor of TNF-alpha, reduces replication of HIV-1 and Mycobacterium tuberculosis in human cells","volume":"3","author":"Haraguchi","year":"2006","journal-title":"AIDS Res. Ther."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1465","DOI":"10.1016\/S0969-2126(96)00153-0","article-title":"Structure of UDP-N-acetylglucosamine enolpyruvyl transferase, an enzyme essential for the synthesis of bacterial peptidoglycan, complexed with substrate UDP-N-acetylglucosamine and the drug fosfomycin","volume":"4","author":"Skarzynski","year":"1996","journal-title":"Structure"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"3755","DOI":"10.1021\/acs.jmedchem.6b01834","article-title":"Discovery and optimization of isoquinoline ethyl ureas as antibacterial agents","volume":"60","author":"Panchaud","year":"2017","journal-title":"J. Med. Chem."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Bhachoo, J., and Beuming, T. (2017). Investigating protein\u2013peptide interactions using the Schr\u00f6dinger computational suite. Modeling Peptide-Protein Interactions: Methods and Protocols, Humana Press.","DOI":"10.1007\/978-1-4939-6798-8_14"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Ali, M.S., Sayem, S.A.J., Quah, Y., Lee, E.-B., Birhanu, B.T., Suk, K., and Park, S.-C. (2021). Investigation of potential antioxidant, thrombolytic and neuropharmacological activities of Homalomena aromatica leaves using experimental and in silico approaches. Molecules, 26.","DOI":"10.3390\/molecules26040975"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1111\/j.1529-8019.2007.00144.x","article-title":"Skin lightening preparations and the hydroquinone controversy","volume":"20","author":"Draelos","year":"2007","journal-title":"Dermatol. Ther."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1605","DOI":"10.1021\/np020128p","article-title":"New antioxidant hydroquinone derivatives from the algicolous marine fungus Acremonium sp.","volume":"65","author":"Fisch","year":"2002","journal-title":"J. Nat. Prod."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"754","DOI":"10.1039\/c0gc00401d","article-title":"5-Hydroxymethylfurfural (HMF) as a building block platform: Biological properties, synthesis and synthetic applications","volume":"13","author":"Rosatella","year":"2011","journal-title":"Green Chem."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1007\/s00217-011-1527-4","article-title":"On the role of 2, 3-dihydro-3, 5-dihydroxy-6-methyl-(4 H)-pyran-4-one in antioxidant capacity of prunes","volume":"233","author":"Cejpek","year":"2011","journal-title":"Eur. Food Res. Technol."},{"key":"ref_33","first-page":"907","article-title":"The inhibitory effect and possible mechanisms of D-allose on cancer cell proliferation","volume":"27","author":"Sui","year":"2005","journal-title":"Int. J. Oncol."},{"key":"ref_34","first-page":"35","article-title":"GC-MS analysis of bioactive compounds in methanolic extract of Holigarna grahamii (wight) Kurz","volume":"35","author":"Jadhav","year":"2014","journal-title":"IJHM"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1515\/jcim-2015-0106","article-title":"Biochemical fingerprint and pharmacological applications of Barleria noctiflora Lf leaves","volume":"13","author":"Yadav","year":"2016","journal-title":"J. Complement. Integr. Med."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1007\/s11046-019-00341-y","article-title":"2, 5-Dimethyl-4-hydroxy-3 (2H)-furanone as an Anti-biofilm Agent Against Non-Candida a lbicans Candida Species","volume":"184","author":"Devadas","year":"2019","journal-title":"Mycopathologia"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Halstead, F.D., Rauf, M., Moiemen, N.S., Bamford, A., Wearn, C.M., Fraise, A.P., Lund, P.A., Oppenheim, B.A., and Webber, M.A. (2015). The antibacterial activity of acetic acid against biofilm-producing pathogens of relevance to burns patients. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0136190"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1016\/j.jhin.2013.05.001","article-title":"The antibacterial activity and stability of acetic acid","volume":"84","author":"Fraise","year":"2013","journal-title":"J. Hosp. Infect."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Debonne, E., Vermeulen, A., Bouboutiefski, N., Ruyssen, T., Van Bockstaele, F., Eeckhout, M., and Devlieghere, F. (2020). Modelling and validation of the antifungal activity of DL-3-phenyllactic acid and acetic acid on bread spoilage moulds. Food Microbiol., 88.","DOI":"10.1016\/j.fm.2019.103407"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"4353","DOI":"10.1021\/jf990140w","article-title":"Volatile flavor components of rice cakes","volume":"47","author":"Buttery","year":"1999","journal-title":"J. Agric. Food Chem."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1007\/s11243-009-9250-8","article-title":"A study of mercury (II)-catalysed substitution of cyanide in hexacyanoferrate (II) by 2-methylpyrazine: Effects of inhibitor on the catalysed reaction","volume":"34","author":"Bansal","year":"2009","journal-title":"Transit. Met. Chem."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1007\/s00107-016-1057-7","article-title":"Determining the curing parameters of furfuryl alcohol for wood modification by nanoindentation","volume":"75","author":"Li","year":"2017","journal-title":"Eur. J. Wood Wood Prod."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1033","DOI":"10.1007\/BF01915330","article-title":"Induction of electrical excitability in crustacean muscle by 4-cyclopentene-1, 3-dione","volume":"34","year":"1978","journal-title":"Experientia"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1007\/BF00437648","article-title":"Antimicrobial activity of protoanemonin, a lactone from ranunculaceous plants","volume":"98","author":"Mares","year":"1987","journal-title":"Mycopathologia"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1007\/s00213-009-1477-8","article-title":"Behavioral effects and pharmacokinetics of gamma-hydroxybutyrate (GHB) precursors gamma-butyrolactone (GBL) and 1, 4-butanediol (1, 4-BD) in baboons","volume":"204","author":"Goodwin","year":"2009","journal-title":"Psychopharmacology"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1007\/BF02150818","article-title":"The effect of 4-amino-5-imidazolecarboxamide on the synergistic antineoplastic activity of 6-chloropurine and azaserine","volume":"21","author":"Sartorelli","year":"1965","journal-title":"Experientia"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1213\/ANE.0000000000005576","article-title":"Volutrauma Increases Exhaled Pentanal in Rats: A Potential Breath Biomarker for Ventilator-Induced Lung Injury","volume":"133","author":"Kiefer","year":"2021","journal-title":"Anesth. Analg."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1007\/s10517-018-3984-2","article-title":"Diagnostics of early changes in the immune system due to low concentration of N-nitrosamines in the Blood","volume":"164","author":"Zaitseva","year":"2018","journal-title":"Bull. Exp. Biol. Med."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Xue, C.-H., Bai, X., and Jia, S.-T. (2016). Robust, self-healing superhydrophobic fabrics prepared by one-step coating of PDMS and octadecylamine. Sci. Rep., 6.","DOI":"10.1038\/srep27262"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1080\/14786419.2013.850686","article-title":"Bioactive metabolites isolated from Penicillium sp. YY-20, the endophytic fungus from Ginkgo biloba","volume":"28","author":"Yuan","year":"2014","journal-title":"Nat. Prod. Res."},{"key":"ref_51","first-page":"220","article-title":"The biological activity of phenolic compounds. The effect of surface active substances upon the penetration of hexyl resorcinol into Ascaris lumbricoides var. suis","volume":"133","author":"Alexander","year":"1946","journal-title":"Proc. R. Soc. London Ser. B-Biol. Sci."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1021\/ja01435a014","article-title":"The preparation of some alkyl derivatives of resorcinol and the relation of their structure to antiseptic properties","volume":"43","author":"Johnson","year":"1921","journal-title":"J. Am. Chem. Soc."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1780","DOI":"10.1271\/bbb.58.1780","article-title":"A simple screening method for antioxidants and isolation of several antioxidants produced by marine bacteria from fish and shellfish","volume":"58","author":"Takao","year":"1994","journal-title":"Biosci. Biotechnol. Biochem."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.cryobiol.2007.05.003","article-title":"Cryoprotective effects of D-allose on mammalian cells","volume":"55","author":"Sui","year":"2007","journal-title":"Cryobiology"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1111\/j.1600-0773.1979.tb02361.x","article-title":"Penetration enhancers and other factors governing percutaneous local anaesthesia with lidocaine","volume":"45","author":"Haegerstam","year":"1979","journal-title":"Acta Pharmacol. Et Toxicol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1016\/j.apcata.2004.09.031","article-title":"Selective synthesis of cis-2, 6-dimethylpiperazine catalyzed by a Cu-Cr-Fe\/\u03b3-Al2O3 catalyst","volume":"277","author":"Bai","year":"2004","journal-title":"Appl. Catal. A Gen."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"671","DOI":"10.3109\/14756366.2014.959947","article-title":"Preparation, anticholinesterase activity, and docking study of new 2-butenediamide and oxalamide derivatives","volume":"30","author":"Yerdelen","year":"2015","journal-title":"J. Enzym. Inhib. Med. Chem."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"20","DOI":"10.13005\/ojc\/320207","article-title":"Antibacterial effect of ginger (Zingiber officinale) roscoe and bioactive chemical analysis using gas chromatography mass spectrum","volume":"32","author":"Shareef","year":"2016","journal-title":"Orient. J. Chem."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1163\/003925995X00099","article-title":"Toxicity in relation to structure of substituted 2H-1-benzopyran-2-ones against root-knot and seed-gall nematodes","volume":"41","author":"Malik","year":"1995","journal-title":"Nematologica"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"1229","DOI":"10.1007\/s40265-018-0955-x","article-title":"Perioperative use of intravenous lidocaine","volume":"78","author":"Beaussier","year":"2018","journal-title":"Drugs"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/S0304-3959(00)00229-3","article-title":"Systemic lidocaine for neuropathic pain relief","volume":"87","author":"Mao","year":"2000","journal-title":"Pain"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1002\/jobm.202100061","article-title":"Antibacterial activities of hexadecanoic acid methyl ester and green-synthesized silver nanoparticles against multidrug-resistant bacteria","volume":"61","author":"Shaaban","year":"2021","journal-title":"J. Basic Microbiol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1080\/07352689.2011.616039","article-title":"Review scopoletin\u2014A coumarin phytoalexin with medicinal properties","volume":"31","author":"Gnonlonfin","year":"2012","journal-title":"Crit. Rev. Plant Sci."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"854","DOI":"10.1080\/13880200802367155","article-title":"Anti-inflammatory effects of scopoletin and underlying mechanisms","volume":"46","author":"Ding","year":"2008","journal-title":"Pharm. Biol."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"2025","DOI":"10.3109\/03639049409049335","article-title":"Quantitation of activity of alkyl ester prodrugs of ibuprofen","volume":"20","author":"Bansal","year":"1994","journal-title":"Drug Dev. Ind. Pharm."},{"key":"ref_66","unstructured":"Buitelaar, R., Bucke, C., Tramper, J., and Wijffels, R. (1996). Immobilized Cells: Basics and Applications, Elsevier."},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Kim, B.-R., Kim, H.M., Jin, C.H., Kang, S.-Y., Kim, J.-B., Jeon, Y.G., Park, K.Y., Lee, I.-S., and Han, A.-R. (2020). Composition and antioxidant activities of volatile organic compounds in radiation-bred Coreopsis cultivars. Plants, 9.","DOI":"10.3390\/plants9060717"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"S4","DOI":"10.1016\/j.jfca.2008.12.002","article-title":"Conjugated linoleic acid (CLA): Good or bad trans fat?","volume":"22","author":"Park","year":"2009","journal-title":"J. Food Compos. Anal."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"550","DOI":"10.6026\/97320630017550","article-title":"Molecular docking analysis of CDK-1 inhibitors from Chrysophyllum cainito leaves","volume":"17","author":"Prabhu","year":"2021","journal-title":"Bioinformation"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1016\/j.sjbs.2015.10.026","article-title":"Characteristics of antibacterial molecular activities in poplar wood extractives","volume":"24","author":"Peng","year":"2017","journal-title":"Saudi J. Biol. Sci."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1016\/j.phymed.2006.03.010","article-title":"Effects of Hydrocotyle sibthorpioides extract on transplanted tumors and immune function in mice","volume":"14","author":"Yu","year":"2007","journal-title":"Phytomedicine"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"721","DOI":"10.21608\/jesp.2018.76592","article-title":"Acute larvicidal toxicity and repellency effect of octopus cyanea crude extracts against the filariasis vector, culex pipiens","volume":"48","author":"Hasaballah","year":"2018","journal-title":"J. Egypt. Soc. Parasitol."},{"key":"ref_73","doi-asserted-by":"crossref","unstructured":"\u00d6zogul, \u0130., Kuley, E., Ucar, Y., Yazgan, H., and \u00d6zogul, Y. (2021). Inhibitory impacts of Spirulina platensis and Chlorella vulgaris extracts on biogenic amine accumulation in sardine fillets. Food Biosci., 41.","DOI":"10.1016\/j.fbio.2021.101087"},{"key":"ref_74","doi-asserted-by":"crossref","unstructured":"Zinn, M.-K., and Bockm\u00fchl, D. (2020). Did granny know best? Evaluating the antibacterial, antifungal and antiviral efficacy of acetic acid for home care procedures. BMC Microbiol., 20.","DOI":"10.1186\/s12866-020-01948-8"},{"key":"ref_75","doi-asserted-by":"crossref","unstructured":"Srivastava, R.P., Kumar, S., Singh, L., Madhukar, M., Singh, N., Saxena, G., Pandey, S., Singh, A., Devkota, H.P., and Verma, P.C. (2023). Major phenolic compounds, antioxidant, antimicrobial, and cytotoxic activities of Selinum carvifolia (L.) collected from different altitudes in India. Front. Nutr., 10.","DOI":"10.3389\/fnut.2023.1180225"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"1036","DOI":"10.1016\/S0021-9258(18)96229-0","article-title":"Studies on the Chemical Modification of Arginine: I. The reaction of 1, 2-cyclohexanedione with arginine and arginyl residues of proteins","volume":"242","author":"Toi","year":"1967","journal-title":"J. Biol. Chem."},{"key":"ref_77","first-page":"181","article-title":"Predicting the biological activities of 2-methoxyphenol antioxidants: Effects of dimers","volume":"21","author":"Fujisawa","year":"2007","journal-title":"In Vivo"},{"key":"ref_78","doi-asserted-by":"crossref","unstructured":"Bako, B., Etim, E.E., Shinggu, J.P., Samuel, H.S., and Moses, L.J. (2025, January 08). Computational insights of 1-Guanidinosuccinimide and Benzene-ethanamine, 2, 5-difluoro-\u03b2-3, 4-trihydroxy-n-methyl with MDM2 as Potential Anticancer Agent. Available online: https:\/\/papers.ssrn.com\/sol3\/papers.cfm?abstract_id=4649989.","DOI":"10.21203\/rs.3.rs-4653936\/v1"},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"34456","DOI":"10.1039\/D1RA06317K","article-title":"Effect of hydroxyl on antioxidant properties of 2, 3-dihydro-3, 5-dihydroxy-6-methyl-4 H-pyran-4-one to scavenge free radicals","volume":"11","author":"Chen","year":"2021","journal-title":"RSC Adv."},{"key":"ref_80","doi-asserted-by":"crossref","unstructured":"Giner, R.M., R\u00edos, J.L., and M\u00e1\u00f1ez, S. (2022). Antioxidant activity of natural hydroquinones. Antioxidants, 11.","DOI":"10.3390\/antiox11020343"},{"key":"ref_81","unstructured":"NIST (2025, January 08). NIST Chemistry WebBook, SRD 69, Available online: https:\/\/webbook.nist.gov\/cgi\/inchi?ID=C1941124&Mask=2000."},{"key":"ref_82","doi-asserted-by":"crossref","unstructured":"Garg, P., Awasthi, S., Horne, D., Salgia, R., and Singhal, S.S. (2023). The innate effects of plant secondary metabolites in preclusion of gynecologic cancers: Inflammatory response and therapeutic action. Biochim. Et Biophys. Acta (BBA)-Rev. Cancer, 1878.","DOI":"10.1016\/j.bbcan.2023.188929"},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"369","DOI":"10.32628\/IJSRST18401159","article-title":"Synthesis and antibacterial activity of novel 3-hydroxy benzoic acid hybrid derivative [Part I]","volume":"4","author":"Satpute","year":"2018","journal-title":"Int. J. Sci. Res. Sci. Technol."},{"key":"ref_84","doi-asserted-by":"crossref","unstructured":"Gao, T., Zhang, Y., Shi, J., Mohamed, S.R., Xu, J., and Liu, X. (2021). The antioxidant guaiacol exerts fungicidal activity against fungal growth and deoxynivalenol production in Fusarium graminearum. Front. Microbiol., 12.","DOI":"10.3389\/fmicb.2021.762844"},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/j.procbio.2021.07.018","article-title":"Production of antimicrobial powders of guaiacol oligomers by a laccase-catalyzed synthesis reaction","volume":"111","author":"Li","year":"2021","journal-title":"Process Biochem."},{"key":"ref_86","doi-asserted-by":"crossref","unstructured":"Tomi\u0107, A., \u0160ovljanski, O., Nikoli\u0107, V., Pezo, L., A\u0107imovi\u0107, M., Cvetkovi\u0107, M., Stanojev, J., Kuzmanovi\u0107, N., and Markov, S. (2023). Screening of antifungal activity of essential oils in controlling biocontamination of historical papers in archives. Antibiotics, 12.","DOI":"10.3390\/antibiotics12010103"},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"106283","DOI":"10.1016\/j.ultsonch.2022.106283","article-title":"Ultrasonic assisted extraction of polyphenols from bayberry by deep eutectic supramolecular polymer and its application in bio-active film","volume":"92","author":"Shi","year":"2023","journal-title":"Ultrason. Sonochem."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"179","DOI":"10.22146\/ijc.21256","article-title":"Biotransformation and cytotoxic activity of guaiacol dimer","volume":"14","author":"Widiyarti","year":"2014","journal-title":"Indones. J. Chem."},{"key":"ref_89","unstructured":"Addie, D.D. (2025, January 08). Phenols and Related Compounds as Antiseptics and Disinfectants for Use with Animals. Available online: https:\/\/www.merckvetmanual.com\/pharmacology\/antiseptics-and-disinfectants\/phenols-and-related-compounds-as-antiseptics-and-disinfectants-for-use-with-animals."},{"key":"ref_90","doi-asserted-by":"crossref","unstructured":"Kouakou, K., Panda, S.K., Yang, M.-R., Lu, J.-G., Jiang, Z.-H., Van Puyvelde, L., and Luyten, W. (2019). Isolation of antimicrobial compounds from Cnestis ferruginea Vahl ex. DC (Connaraceae) leaves through bioassay-guided fractionation. Front. Microbiol., 10.","DOI":"10.3389\/fmicb.2019.00705"},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1007\/s10096-024-05009-0","article-title":"3-Hydroxybenzoic acid inhibits the virulence attributes and disrupts biofilm production in clinical isolates of A cinetobacter baumannii","volume":"44","author":"Pathoor","year":"2024","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"ref_92","doi-asserted-by":"crossref","unstructured":"Lima, L.G.B., Montenegro, J., Abreu, J.P.d., Santos, M.C.B., Nascimento, T.P.d., Santos, M.d.S., Ferreira, A.G., Cameron, L.C., Ferreira, M.S.L., and Teodoro, A.J. (2020). Metabolite profiling by UPLC-MSE, NMR, and antioxidant properties of Amazonian fruits: Mamey Apple (Mammea americana), Camapu (Physalis angulata), and Uxi (Endopleura uchi). Molecules, 25.","DOI":"10.3390\/molecules25020342"},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1016\/j.burns.2008.11.009","article-title":"The antimicrobial effect of acetic acid\u2014An alternative to common local antiseptics?","volume":"35","author":"Ryssel","year":"2009","journal-title":"Burns"},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"45","DOI":"10.3746\/jfn.2011.16.1.045","article-title":"Profiling of volatile components using gas chromatography-mass spectrometry in commercial pine needle (Pinus densiflora S. and Z.) powder","volume":"16","author":"Kim","year":"2011","journal-title":"Prev. Nutr. Food Sci."},{"key":"ref_95","doi-asserted-by":"crossref","unstructured":"S\u00e1nchez-Moya, T., L\u00f3pez-Nicol\u00e1s, R., Peso-Echarri, P., Gonz\u00e1lez-Berm\u00fadez, C.A., and Frontela-Saseta, C. (2024). Effect of pine bark extract and its phenolic compounds on selected pathogenic and probiotic bacterial strains. Front. Nutr., 11.","DOI":"10.3389\/fnut.2024.1381125"},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"106830","DOI":"10.1016\/j.micpath.2024.106830","article-title":"Methyl gallate attenuates virulence and decreases antibiotic resistance in extensively drug-resistant Pseudomonas aeruginosa","volume":"194","year":"2024","journal-title":"Microb. Pathog."}],"container-title":["Antioxidants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-3921\/14\/9\/1114\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:45:17Z","timestamp":1760035517000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-3921\/14\/9\/1114"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9,13]]},"references-count":96,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2025,9]]}},"alternative-id":["antiox14091114"],"URL":"https:\/\/doi.org\/10.3390\/antiox14091114","relation":{},"ISSN":["2076-3921"],"issn-type":[{"value":"2076-3921","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,9,13]]}}}