{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T04:46:04Z","timestamp":1761367564672,"version":"build-2065373602"},"reference-count":53,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T00:00:00Z","timestamp":1761177600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>This study explores the photoprotective and antioxidant potential of cosmetic emulsions formulated with Alnus glutinosa (black alder) extracts. Extraction of bioactive compounds was performed using Soxhlet, ultrasound-assisted, and microwave-assisted techniques with ethanol and water as solvents. The phytochemical profiles of the resulting extracts were characterized via UV-Vis spectroscopy, Fourier-transform infrared spectroscopy (FTIR), and liquid chromatography\u2013high-resolution mass spectrometry (LC-HRMS). The extracts were incorporated into oil-in-water emulsions and assessed for antioxidant activity using the DPPH radical scavenging assay, pH and viscosity stability, and color L*a*b* values. Among the extraction methods, ethanol-based Soxhlet extraction yielded the highest concentration of bioactive compounds and demonstrated superior antioxidant and photoprotective efficacy. This is the first report that evaluates the antioxidant properties of A. glutinosa-enriched emulsions, supporting their application as multifunctional, plant-derived cosmeceuticals for skin protection.<\/jats:p>","DOI":"10.3390\/antiox14111275","type":"journal-article","created":{"date-parts":[[2025,10,24]],"date-time":"2025-10-24T00:47:36Z","timestamp":1761266856000},"page":"1275","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["From Plant to Skin: Exploring Alnus glutinosa Extracts for Cosmeceutical Applications"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0751-2802","authenticated-orcid":false,"given":"Nikolaos D.","family":"Bikiaris","sequence":"first","affiliation":[{"name":"Laboratory of Polymer Chemistry and Technology, Department of Chemistry, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1732-965X","authenticated-orcid":false,"given":"Evangelia","family":"Balla","sequence":"additional","affiliation":[{"name":"Laboratory of Polymer Chemistry and Technology, Department of Chemistry, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-8590-1593","authenticated-orcid":false,"given":"Despoina","family":"Varitimidou","sequence":"additional","affiliation":[{"name":"Laboratory of Polymer Chemistry and Technology, Department of Chemistry, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7187-992X","authenticated-orcid":false,"given":"Lelouda-Athanasia","family":"Koronaiou","sequence":"additional","affiliation":[{"name":"Laboratory of Environmental Pollution Control, Department of Chemistry, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nikolaos","family":"Nikolaidis","sequence":"additional","affiliation":[{"name":"Laboratory of Polymer Chemistry and Technology, Department of Chemistry, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,10,23]]},"reference":[{"key":"ref_1","first-page":"165","article-title":"Evaluation of antioxidant potential and reduction capacity of some plant extracts in silver nanoparticles\u2019 synthesis","volume":"3","author":"Goodarzi","year":"2014","journal-title":"Mol. Biol. Res. Commun."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Irshad, A., Jawad, R., Mushtaq, Q., Spalletta, A., Martin, P., and Ishtiaq, U. (2025). Determination of antibacterial and antioxidant potential of organic crude extracts from Malus domestica, Cinnamomum verum and Trachyspermum ammi. Sci. Rep., 15.","DOI":"10.1038\/s41598-024-83506-0"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Kasote, D.M., Katyare, S.S., Hegde, M.V., and Bae, H. (2015). Significance of antioxidant potential of plants and its relevance to therapeutic applications. Int. J. Biol. Sci., 11.","DOI":"10.7150\/ijbs.12096"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Hoang, H.T., Moon, J.Y., and Lee, Y.C. (2021). Natural antioxidants from plant extracts in skincare cosmetics: Recent applications, challenges and perspectives. Cosmetics, 8.","DOI":"10.3390\/cosmetics8040106"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Salmon, V.G., Breen, A.L., Kumar, J., Lara, M.J., Thornton, P.E., Wullschleger, S.D., and Iversen, C.M. (2019). Alder distribution and expansion across a tundra hillslope: Implications for local N cycling. Front. Plant Sci., 10.","DOI":"10.3389\/fpls.2019.01099"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"879","DOI":"10.1007\/s00107-019-01446-3","article-title":"Comparative study on radical scavenging activity and phenolic compounds content in water bark extracts of alder (Alnus glutinosa (L.) Gaertn.), oak (Quercus robur L.) and pine (Pinus sylvestris L.)","volume":"77","author":"Skrypnik","year":"2019","journal-title":"Eur. J. Wood Wood Prod."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Ren, X., He, T., Chang, Y., Zhao, Y., Chen, X., Bai, S., Wang, L., Shen, M., and She, G. (2017). The genus Alnus, a comprehensive outline of its chemical constituents and biological activities. Molecules, 22.","DOI":"10.3390\/molecules22081383"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1088","DOI":"10.1055\/s-0034-1382993","article-title":"Antioxidative Activity of Diarylheptanoids from the Bark of Black Alder (Alnus glutinosa) and Their Interaction with Anticancer Drugs","volume":"80","author":"Vajs","year":"2014","journal-title":"Planta Medica"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Sukhikh, S., Ivanova, S., Skrypnik, L., Bakhtiyarova, A., Larina, V., Krol, O., Prosekov, A., Frolov, A., Povydysh, M., and Babich, O. (2022). Study of the Antioxidant Properties of Filipendula ulmaria and Alnus glutinosa. Plants, 11.","DOI":"10.3390\/plants11182415"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1111\/j.1600-0846.2011.00588.x","article-title":"Methods for evaluation of cosmetic antioxidant capacity","volume":"18","author":"Arct","year":"2012","journal-title":"Ski. Res. Technol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1548","DOI":"10.1055\/s-2008-1081296","article-title":"Phytochemicals as protectors against ultraviolet radiation: Versatility of effects and mechanisms","volume":"74","year":"2008","journal-title":"Planta Medica"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1007","DOI":"10.1016\/0014-3057(77)90173-2","article-title":"Mechanisms of antioxidant action: Synergism between antioxidants and \u2018UV absorbers\u2019","volume":"13","author":"Chakraborty","year":"1977","journal-title":"Eur. Polym. J."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Schuster, A., Ortmayr, N., Oostingh, G.J., and Stelzhammer, B. (2020). Compounds Extracted from Larch, Birch bark, Douglas Fir, and Alder Woods with Four Different Solvents: Effects on Five Skin-related Microbes. BioResources, 15.","DOI":"10.15376\/biores.15.2.3368-3381"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1825","DOI":"10.1007\/s12221-024-00535-2","article-title":"Dyeing of Cotton and Wool Fibers with the Aqueous Extract of Alnus glutinosa: Evaluation of Their Ultraviolet Protection Factor, Their Color fastness and the Antioxidant Activity of the Aqueous Extract","volume":"25","author":"Tsouka","year":"2024","journal-title":"Fibers Polym."},{"key":"ref_15","first-page":"100","article-title":"Antimicrobial and cytotoxic activity of Alnus glutinosa (L.) Gaertn., A. incana (L.) Moench, and A. viridis (Chaix) DC. extracts","volume":"6","author":"Dahija","year":"2016","journal-title":"J. Health Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/S0255-2701(02)00037-5","article-title":"Microwave-assisted extraction of tea polyphenols and tea caffeine from green tea leaves","volume":"42","author":"Pan","year":"2003","journal-title":"Chem. Eng. Process. Process. Intensif."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2097","DOI":"10.1021\/es5002105","article-title":"Identifying small molecules via high resolution mass spectrometry: Communicating confidence","volume":"48","author":"Schymanski","year":"2014","journal-title":"Environ. Sci. Technol."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Bikiaris, N.D., Michailidou, G., Lazaridou, M., Christodoulou, E., Gounari, E., Ofrydopoulou, A., Lambropoulou, D.A., Vergkizi-Nikolakaki, S., Lykidou, S., and Nikolaidis, N. (2020). Innovative skin product emulsions with enhanced antioxidant, antimicrobial and UV protection properties containing nanoparticles of pure and modified Chitosan with encapsulated fresh pomegranate juice. Polymers, 12.","DOI":"10.3390\/polym12071542"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Dalla, E., Koumentakou, I., Bikiaris, N., Balla, E., Lykidou, S., and Nikolaidis, N. (2022). Formulation, Characterization and Evaluation of Innovative O\/W Emulsions Containing Curcumin Derivatives with Enhanced Antioxidant Properties. Antioxidants, 11.","DOI":"10.3390\/antiox11112271"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3390\/applnano3010001","article-title":"Innovative Skin Product O\/W Emulsions Containing Lignin, Multiwall Carbon Nanotubes and Graphene Oxide Nanoadditives with Enhanced Sun Protection Factor and UV Stability Properties","volume":"3","author":"Bikiaris","year":"2022","journal-title":"Appl. Nano"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1111\/j.1745-4573.2007.00067.x","article-title":"Pork color measurement as affected by bloom time and measurement location","volume":"18","year":"2007","journal-title":"J. Muscle Foods"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"121370","DOI":"10.1016\/j.ijpharm.2021.121370","article-title":"Relationship between sensorial and physical characteristics of topical creams: A comparative study on effects of excipients","volume":"613","author":"Ali","year":"2022","journal-title":"Int. J. Pharm."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/S0927-7757(98)00626-8","article-title":"Rheological studies to objectify sensations occurring when cosmetic emulsions are applied to the skin","volume":"152","author":"Brummer","year":"1999","journal-title":"Eng. Asp."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Pagels, F., Almeida, C., Vasconcelos, V., and Guedes, A.C. (2022). Cosmetic Potential of Pigments Extracts from the Marine Cyanobacterium Cyanobium sp.. Mar. Drugs, 20.","DOI":"10.3390\/md20080481"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"3059","DOI":"10.1007\/s10811-021-02522-z","article-title":"The application of a phycocyanin extract obtained from Arthrospira platensis as a blue natural colorant in beverages","volume":"33","author":"Longo","year":"2021","journal-title":"J. Appl. Phycol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"e17129","DOI":"10.1590\/s2175-97902019000417129","article-title":"Effect of solvent polarity on extraction yield and antioxidant properties of phytochemicals from bean (Phaseolus vulgaris) seeds","volume":"56","author":"Nawaz","year":"2020","journal-title":"Braz. J. Pharm. Sci."},{"key":"ref_27","first-page":"1147","article-title":"Effects of solvent type on phenolics and flavonoids content and antioxidant activities in two varieties of young ginger (Zingiber officinale Roscoe) extracts","volume":"5","author":"Ghasemzadeh","year":"2011","journal-title":"J. Med. Plants Res."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1080\/0028825X.2024.2392705","article-title":"Solvent polarity effects on extraction yield, phenolic content, and antioxidant properties of Malvaceae family seeds: A comparative study","volume":"63","author":"Ghaffar","year":"2024","journal-title":"N. Z. J. Bot."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.foodchem.2011.08.034","article-title":"UV\/Visible spectra of natural polyphenols: A time-dependent density functional theory study","volume":"131","author":"Anouar","year":"2012","journal-title":"Food Chem."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.supflu.2011.10.003","article-title":"Supercritical fluid extraction of Alnus glutinosa (L.) Gaertn","volume":"61","author":"Szarka","year":"2012","journal-title":"J. Supercrit. Fluids"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"776","DOI":"10.1021\/je700633w","article-title":"Solubility of gallic acid in methanol, ethanol, water, and ethyl acetate","volume":"53","author":"Daneshfar","year":"2008","journal-title":"J. Chem. Eng. Data"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2191","DOI":"10.1021\/ed500563w","article-title":"Investigating Hydrogen Bonding in Phenol Using Infrared Spectroscopy and Computational Chemistry","volume":"91","author":"Fedor","year":"2014","journal-title":"J. Chem. Educ."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Kledecka, A., Siejak, P., Pratap-Singh, A., Kowalczewski, P.\u0141., Fathordoobady, F., Jarz\u0119bski, M., and Smu\u0142ek, W. (2022). Extracts from Frangula alnus Mill. and Their Effects on Environmental and Probiotic Bacteria. Plants, 11.","DOI":"10.3390\/plants11202719"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/j.jphotobiol.2015.02.003","article-title":"Photocatalytic destruction of Escherichia coli in water by V2O5\/TiO2","volume":"144","author":"Sethi","year":"2015","journal-title":"J. Photochem. Photobiol. B"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1515\/hf.2011.096","article-title":"Structure and antioxidant activity of diarylheptanoids extracted from bark of grey alder (Alnus incana) and potential of biorefinery-based bark processing of European trees","volume":"65","author":"Telysheva","year":"2011","journal-title":"Holzforschung"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1159\/000489522","article-title":"PH and Microbial Infections","volume":"54","author":"Rippke","year":"2018","journal-title":"Curr. Probl. Dermatol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"9","DOI":"10.5650\/jos.62.9","article-title":"The viscosity stability of O\/W emulsion containing \u03b1-gel through an ionic-complex system","volume":"62","author":"Uyama","year":"2013","journal-title":"J. Oleo Sci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"938042","DOI":"10.1155\/2013\/938042","article-title":"Assessment of physical stability and antioxidant activity of polysiloxane polyalkyl polyether copolymer-based creams","volume":"2013","author":"Ali","year":"2013","journal-title":"J. Chem."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1016\/j.jpba.2004.10.030","article-title":"Assays of physical stability and antioxidant activity of a topical formulation added with different plant extracts","volume":"37","author":"Fonseca","year":"2005","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"13","DOI":"10.22159\/ijap.2018v10i5.28102","article-title":"A review on herbal cosmetics in Indonesia","volume":"10","author":"Wathoni","year":"2018","journal-title":"Int. J. Appl. Pharm."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Hussen, E.M., and Endalew, S.A. (2023). In vitro antioxidant and free-radical scavenging activities of polar leaf extracts of Vernonia amygdalina. BMC Complement. Med. Ther., 23.","DOI":"10.1186\/s12906-023-03923-y"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1186\/1472-6882-14-145","article-title":"Phenolic contents, antioxidant and anticholinesterase potentials of crude extract, subsequent fractions and crude saponins from Polygonum hydropiper L.","volume":"14","author":"Ayaz","year":"2014","journal-title":"BMC Complement. Altern. Med."},{"key":"ref_43","first-page":"75","article-title":"In Vitro Antioxidant Activity of the Whole Plant of Amaranthus spinosus Linn","volume":"5","author":"Baral","year":"2010","journal-title":"Int. J. Biomed. Pharm. Sci."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Imamovi\u0107, B., Ivazovi\u0107, I., Alispahi\u0107, A., Be\u010di\u0107, E., Dedi\u0107, M., and Daci\u0107, A. (2021). Assessment of the suitability of methods for testing the antioxidant activity of anti-aging creams. Appl. Sci., 11.","DOI":"10.3390\/app11041358"},{"key":"ref_45","first-page":"127","article-title":"Effect of Moringa oleifera on undesireble skin sebum secretions of sebaceous glands observed during winter season in humans","volume":"24","author":"Ali","year":"2013","journal-title":"Biomed Res."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Smeriglio, A., D\u2019angelo, V., Cacciola, A., Ingegneri, M., Raimondo, F.M., Trombetta, D., and German\u00f2, M.P. (2022). New Insights on Phytochemical Features and Biological Properties of Alnus glutinosa Stem Bark. Plants, 11.","DOI":"10.3390\/plants11192499"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Angeli, L., Imperiale, S., Ding, Y., Scampicchio, M., and Morozova, K. (2021). A novel stoichio-kinetic model for the DPPH\u2022 assay: The importance of the side reaction and application to complex mixtures. Antioxidants, 10.","DOI":"10.3390\/antiox10071019"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1036","DOI":"10.1016\/j.foodchem.2011.07.127","article-title":"Estimation of antiradical properties of antioxidants using DPPH- assay: A critical review and results","volume":"130","author":"Mishra","year":"2012","journal-title":"Food Chem."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"12222","DOI":"10.3390\/ijms140612222","article-title":"UV radiation and the skin","volume":"14","author":"Jarrett","year":"2013","journal-title":"Int. J. Mol. Sci."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Rosado, C., Tokunaga, V.K., Sauce, R., de Oliveira, C.A., Sarruf, F.D., Parise-Filho, R., Maur\u00edcio, E., de Almeida, T.S., Velasco, M.V.R., and Baby, A.R. (2019). Another reason for using caffeine in dermocosmetics: Sunscreen adjuvant. Front. Physiol., 10.","DOI":"10.3389\/fphys.2019.00519"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"102000","DOI":"10.1016\/j.jddst.2020.102000","article-title":"Development of resveratrol and green tea sunscreen formulation for combined photoprotective and antioxidant properties","volume":"60","author":"Bhattacharya","year":"2020","journal-title":"J. Drug Deliv. Sci. Technol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1016\/j.lfs.2011.01.010","article-title":"Dietary aloin, aloesin, or aloe-gel exerts anti-inflammatory activity in a rat colitis model","volume":"88","author":"Park","year":"2011","journal-title":"Life Sci."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1002\/ame2.12295","article-title":"Natural products and extracts from plants as natural UV filters for sunscreens: A review","volume":"6","author":"Li","year":"2023","journal-title":"Anim. Model. Exp. Med."}],"container-title":["Antioxidants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-3921\/14\/11\/1275\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T04:41:28Z","timestamp":1761367288000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-3921\/14\/11\/1275"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,23]]},"references-count":53,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2025,11]]}},"alternative-id":["antiox14111275"],"URL":"https:\/\/doi.org\/10.3390\/antiox14111275","relation":{},"ISSN":["2076-3921"],"issn-type":[{"type":"electronic","value":"2076-3921"}],"subject":[],"published":{"date-parts":[[2025,10,23]]}}}