{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T10:24:02Z","timestamp":1787739842426,"version":"build-2784847793"},"reference-count":45,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T00:00:00Z","timestamp":1761350400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Industry-Academia-Research Institute (IAR) Collabo R&D Fund Grant funded by the Korean Government","award":["RS-2025-02313877"],"award-info":[{"award-number":["RS-2025-02313877"]}]},{"name":"PLABiologics Co., Ltd."}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>Age-related macular degeneration (AMD) is a retinal degenerative disease caused by oxidative stress. Thus, we aimed to reduce oxidative stress through the use of placenta-derived mesenchymal stem cells (PD-MSCs). To induce oxidative stress in ARPE-19 cells, we treated them with 200 \u00b5M hydrogen peroxide (H2O2) for 2 h and then cocultured them with PD-MSCs. The dissociation of the KEAP1\/Nrf2 complex, along with the expression of phosphoinositide 3-kinase (PI3K) and protein kinase B (AKT), increased in the coculture group compared with the H2O2 treatment group (* p &lt; 0.05). The expression levels of antioxidant genes increased in the cocultured group compared with those in the H2O2 treatment group (* p &lt; 0.05), whereas the ROS levels decreased in the cocultured group (* p &lt; 0.05). Additionally, both the expression of mitochondrial dynamics markers and the mitochondrial membrane potential increased when the cells were cocultured with PD-MSCs (* p &lt; 0.05). PD-MSC cocultivation decreased the expression levels of lipoproteins (* p &lt; 0.05). Finally, we confirmed that PD-MSCs promoted the expression of RPE-specific genes in H2O2-injured ARPE-19 cells (* p &lt; 0.05). These findings suggest a new aspect of stem cell treatment for AMD induced by oxidative stress.<\/jats:p>","DOI":"10.3390\/antiox14111279","type":"journal-article","created":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T00:51:42Z","timestamp":1761526302000},"page":"1279","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Nrf2 Activated by PD-MSCs Attenuates Oxidative Stress in a Hydrogen Peroxide-Injured Retinal Pigment Epithelial Cell Line"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-0008-9268","authenticated-orcid":false,"given":"Se Jin","family":"Hong","sequence":"first","affiliation":[{"name":"Division of Life Sciences, Department of Life Science, Graduate School, CHA University, Seongnam-si 13488, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3856-5029","authenticated-orcid":false,"given":"Dae-Hyun","family":"Lee","sequence":"additional","affiliation":[{"name":"Division of Life Sciences, Department of Life Science, Graduate School, CHA University, Seongnam-si 13488, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeong Woo","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Biomedical Science, CHA University, Pocheon-si 11160, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hankyu","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Biomedical Science, CHA University, Pocheon-si 11160, Republic of Korea"},{"name":"PLABiologics Co., Ltd., Seongnam-si 13522, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2140-275X","authenticated-orcid":false,"given":"Youngje","family":"Sung","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology CHA Bundang Medical Center, CHA University, Seongnam-si 13488, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2320-7157","authenticated-orcid":false,"given":"Gi Jin","family":"Kim","sequence":"additional","affiliation":[{"name":"Division of Life Sciences, Department of Life Science, Graduate School, CHA University, Seongnam-si 13488, Republic of Korea"},{"name":"Department of Biomedical Science, CHA University, Pocheon-si 11160, Republic of Korea"},{"name":"PLABiologics Co., Ltd., Seongnam-si 13522, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,10,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"942","DOI":"10.1016\/j.survophthal.2025.03.004","article-title":"The role of retinal pigment epithelial senescence and the potential of senotherapeutics in age-related macular degeneration","volume":"70","author":"Chen","year":"2025","journal-title":"Surv. 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