{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T05:15:43Z","timestamp":1762406143594,"version":"build-2065373602"},"reference-count":21,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T00:00:00Z","timestamp":1761868800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>Kunzea ericoides (kanuka), a native plant of New Zealand, has a significant role in traditional medicine due to the presence of essential oils. Apart from these oils, this plant also is a source of many bioactive compounds, majority of which are polyphenols. However, there is lack of sufficient data supporting the extraction of polyphenols from kanuka plant leaves and investigating its bioactivity and phytochemical properties. The study aims to extract polyphenols from kanuka plant leaves with a conventional solvent-based method and determine the phytochemical analysis as well as bioactive potential. Extraction was performed with methanol and acetone as solvents. Polyphenolic prolife was analyzed with LC-MS. Bioactive analysis of kanuka leaf extract was carried out to determine total phenolic content and antioxidant activity. We investigated the cytotoxic effect of kanuka leaf extract on two triple-negative breast cancer cells\u2014MDA-MB-231 and BT-549. LC-MS analysis confirmed kanuka leaf extract is a source of many polyphenols, some giving very prominent signals on TIC scan. Ten polyphenolic compounds were confirmed to be present in kanuka leaf extract based on MRM analysis. FRAP-CUPRAC analysis indicated significant antioxidant activity in the kanuka leaf extract. Antiproliferative analysis has confirmed cytotoxicity of the kanuka leaf extract on the triple-negative breast cancer cell lines. This study indicates that Kunzea ericoides leaf extract, rich in polyphenols, shows promising antioxidant and antiproliferative potential, warranting further investigation for therapeutic applications.<\/jats:p>","DOI":"10.3390\/antiox14111319","type":"journal-article","created":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T13:22:36Z","timestamp":1762176156000},"page":"1319","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Extraction, Phytochemical Analysis, and Bioactivity Evaluation of Polyphenols from Kunzea ericoides (Kanuka) Plant"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-5176-680X","authenticated-orcid":false,"given":"Harmandeep","family":"Dhaliwal","sequence":"first","affiliation":[{"name":"School of Science, Auckland University of Technology, Auckland 1010, New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2781-2119","authenticated-orcid":false,"given":"Yan","family":"Li","sequence":"additional","affiliation":[{"name":"School of Science, Auckland University of Technology, Auckland 1010, New Zealand"},{"name":"Maurice Wilkins Centre, Auckland 1010, New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michelle","family":"Yoo","sequence":"additional","affiliation":[{"name":"School of Science, Auckland University of Technology, Auckland 1010, New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,10,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1016\/S0031-9422(98)00548-2","article-title":"Chemical, physical and antimicrobial properties of essential oils of Leptospermum scoparium and Kunzea ericoides","volume":"50","author":"Porter","year":"1999","journal-title":"Phytochemistry"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"e026201","DOI":"10.1136\/bmjopen-2018-026201","article-title":"Kanuka Honey versus Aciclovir for the Topical Treatment of Herpes Simplex Labialis: A Randomised Controlled Trial","volume":"9","author":"Semprini","year":"2019","journal-title":"BMJ Open"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/j.jmii.2013.12.009","article-title":"Investigations of Kanuka and Manuka Essential Oils for in Vitro Treatment of Disease and Cellular Inflammation Caused by Infectious Microorganisms","volume":"49","author":"Chen","year":"2016","journal-title":"J. Microbiol. Immunol. Infect."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"101561","DOI":"10.1016\/j.eclinm.2022.101561","article-title":"Efficacy of a 3% Kanuka Oil Cream for the Treatment of Moderate-to-Severe Eczema: A Single Blind Randomised Vehicle-Controlled Trial","volume":"51","author":"Shortt","year":"2022","journal-title":"EClinicalMedicine"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/j.ijat.2005.07.003","article-title":"A Fresh Look at Manuka and Kanuka Essential Oils from New Zealand","volume":"15","author":"Wilkinson","year":"2005","journal-title":"Int. J. Aromather."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"7313","DOI":"10.3390\/molecules15107313","article-title":"Plant Phenolics: Extraction, Analysis and Their Antioxidant and Anticancer Properties","volume":"15","author":"Dai","year":"2010","journal-title":"Molecules"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1111\/j.1469-8137.2004.01126.x","article-title":"Differential Accumulation of Flavonoids and Hydroxycinnamates in Leaves of Ligustrum Vulgare under Excess Light and Drought Stress","volume":"163","author":"Tattini","year":"2004","journal-title":"New Phytol."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Chaves, N., Santiago, A., and Al\u00edas, J.C. (2020). Quantification of the Antioxidant Activity of Plant Extracts: Analysis of Sensitivity and Hierarchization Based on the Method Used. 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Prod."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"8892","DOI":"10.1007\/s12010-024-04989-1","article-title":"Kunzea ericoides (Kanuka) Leaf Extracts Show Moisturisation, Antioxidant, and UV Protection Effects in HaCaT Cells and Anti-Melanogenesis Effects in B16F10 Cells","volume":"196","author":"He","year":"2024","journal-title":"Appl. Biochem. Biotechnol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"104721","DOI":"10.1016\/j.supflu.2019.104721","article-title":"Subcritical Water Extraction for Selective Recovery of Phenolic Bioactives from K\u0101nuka Leaves","volume":"158","author":"Essien","year":"2020","journal-title":"J. Supercrit. Fluids"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"140456","DOI":"10.1016\/j.foodchem.2024.140456","article-title":"Extraction of Polyphenols and Essential Oils from Herbs with Green Extraction Methods\u2014An Insightful Review","volume":"460","author":"Hanula","year":"2024","journal-title":"Food Chem."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1821","DOI":"10.1016\/j.foodchem.2010.12.026","article-title":"A Critical Review of Methods for Characterisation of Polyphenolic Compounds in Fruits and Vegetables","volume":"126","author":"Ignat","year":"2011","journal-title":"Food Chem."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Han, Y.K., Vinh, L.B., Nam, M.-h., and Lee, K.Y. (2023). Identification of Compounds Using HPLC-QTOF-MS Online Antioxidant Activity Mapping from Aerial Parts of Ligularia stenocephala. Appl. Biol. Chem., 66.","DOI":"10.1186\/s13765-023-00814-1"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Vinh, L.B., Shin, S.H., Han, Y.K., Kim, Y.J., Cuong, N.C., Oh, S., and Lee, K.Y. (2024). Identification of Interleukin (IL)-33 Inhibitory Constituents from Canavalia gladiata Pods. Antioxidants, 13.","DOI":"10.3390\/antiox13070767"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Diep, T., Pook, C., and Yoo, M. (2020). Phenolic and Anthocyanin Compounds and Antioxidant Activity of Tamarillo (Solanum betaceum Cav.). Antioxidants, 9.","DOI":"10.3390\/antiox9020169"},{"key":"ref_19","first-page":"4563","article-title":"Antioxidant Properties and Composition of Aqueous Extracts from Mentha Species, Hybrids, Varieties, and Cultivars","volume":"51","author":"Dorman","year":"2003","journal-title":"Am. Chem. Soc."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1006\/abio.1996.0292","article-title":"The Ferric Reducing Ability of Plasma (FRAP) as a Measure of \u201c\u2018Antioxidant Power\u2019\u201d: The FRAP Assay","volume":"239","author":"Benzie","year":"1996","journal-title":"Anal. Biochem."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"652","DOI":"10.1016\/j.trac.2010.11.016","article-title":"The Main and Modified CUPRAC Methods of Antioxidant Measurement","volume":"30","author":"Apak","year":"2011","journal-title":"TrAC Trends Anal. Chem."}],"container-title":["Antioxidants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-3921\/14\/11\/1319\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T05:12:14Z","timestamp":1762405934000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-3921\/14\/11\/1319"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,31]]},"references-count":21,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2025,11]]}},"alternative-id":["antiox14111319"],"URL":"https:\/\/doi.org\/10.3390\/antiox14111319","relation":{},"ISSN":["2076-3921"],"issn-type":[{"type":"electronic","value":"2076-3921"}],"subject":[],"published":{"date-parts":[[2025,10,31]]}}}