{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:33:40Z","timestamp":1787027620289,"version":"build-2736575974"},"reference-count":40,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2025,11,20]],"date-time":"2025-11-20T00:00:00Z","timestamp":1763596800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Tian Mengliang Expert Workstation of Yunnan Province\u2014Yunnan Provincial Science and Technology Talent and Platform Program","award":["202505AF350023"],"award-info":[{"award-number":["202505AF350023"]}]},{"name":"National Modern Agricultural Industrial Technology System, Sichuan Innovation Team Project, Genuine Traditional Chinese Medicine Innovation Team","award":["SCCXTD-2025-19"],"award-info":[{"award-number":["SCCXTD-2025-19"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>Dendrobium moschatum neutral polysaccharide (DMP-NP) was isolated using a water extraction\u2013ethanol precipitation method, followed by purification with DEAE-cellulose anion-exchange resin and a dextran gel column. The resulting DMP-NP1 exhibited a weight-average molecular weight of 16.23 kDa. The molar ratio of monosaccharides was as follows: glucose\u2013mannose\u2013galactose\u2013fucose\u2013rhamnose = 78.54:19.11:1.59:0.53:0.23, with a glucose-to-mannose ratio of 4.1:1. Infrared spectroscopic analysis revealed characteristic carbohydrate absorption peaks and confirmed the presence of pyranosidic linkages. NMR analysis revealed that DMP-NP1 possesses a backbone mainly formed by 1\u21924 glycosidic linkages, a small number of 1\u21926 branches, and O-acetyl substitutions at the C2 and C3 positions of mannose residues. In vitro experiments demonstrated that treatment with 0\u201320 \u03bcg\/mL (0\u20131.23 \u03bcM) DMP-NP significantly enhanced the activities of catalase (CAT) and superoxide dismutase (SOD) in IPEC-J2 cells, along with upregulation of the corresponding antioxidant genes. Concurrently, DMP-NP reduced the secretion of key pro-inflammatory cytokines, including TNF-\u03b1, IL-1\u03b2, and IL-6, and downregulated the expression of genes associated with both antioxidant and inflammatory signaling pathways. Collectively, these findings indicate that DMP-NP not only prevents but also ameliorates LPS-induced inflammatory injury in intestinal epithelial cells, thereby providing a basis for the application of DMP-NP in intestinal inflammation mitigation.<\/jats:p>","DOI":"10.3390\/antiox14111384","type":"journal-article","created":{"date-parts":[[2025,11,20]],"date-time":"2025-11-20T14:21:54Z","timestamp":1763648514000},"page":"1384","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Anti-Inflammatory and Antioxidant Mechanisms of Dendrobium moschatum Polysaccharide in Intestinal Epithelial Cells via TLR4-NF-\u03baB and Nrf2 Signaling Pathways"],"prefix":"10.3390","volume":"14","author":[{"given":"Ji","family":"Chen","sequence":"first","affiliation":[{"name":"College of Agronomy, Sichuan Agricultural University, Wenjiang 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunyan","family":"Ma","sequence":"additional","affiliation":[{"name":"College of Agronomy, Sichuan Agricultural University, Wenjiang 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xu","family":"Mo","sequence":"additional","affiliation":[{"name":"College of Agronomy, Sichuan Agricultural University, Wenjiang 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linhong","family":"Li","sequence":"additional","affiliation":[{"name":"Leshan Academy of Agricultural Sciences, Leshan 614000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lijuan","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Agronomy, Sichuan Agricultural University, Wenjiang 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaowen","family":"Zhang","sequence":"additional","affiliation":[{"name":"Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Li","sequence":"additional","affiliation":[{"name":"College of Agronomy, Sichuan Agricultural University, Wenjiang 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3116-1478","authenticated-orcid":false,"given":"Yuanfeng","family":"Zou","sequence":"additional","affiliation":[{"name":"Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fan","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Agronomy, Sichuan Agricultural University, Wenjiang 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengliang","family":"Tian","sequence":"additional","affiliation":[{"name":"College of Agronomy, Sichuan Agricultural University, Wenjiang 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,11,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Zhou, H.L., Zeng, X.J., Sun, D.C., Chen, Z., Chen, W.X., Fan, L.W., Limpanont, Y., Dekumyoy, P., Maleewong, W., and Lv, Z.Y. 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