{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,27]],"date-time":"2025-11-27T16:49:47Z","timestamp":1764262187402,"version":"3.46.0"},"reference-count":26,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2025,11,27]],"date-time":"2025-11-27T00:00:00Z","timestamp":1764201600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"European Union-NextGenerationEU-National Recovery and Resilience Plan","award":["CUP B83C22003920001"],"award-info":[{"award-number":["CUP B83C22003920001"]}]},{"name":"Italian Ministry of Foreign Affairs and International Cooperation","award":["CUP B53C23007370001"],"award-info":[{"award-number":["CUP B53C23007370001"]}]},{"name":"CR Firenze Foundation","award":["CUP B13C23003400007"],"award-info":[{"award-number":["CUP B13C23003400007"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>Infertility is a widespread global problem, with a male factor contributing to approximately 40\u201350% of cases. Several studies have investigated the involvement of adipokines in reproductive functions, but only a few have investigated their role in the male reproductive component. Collectively, adipokines are present in human sperm and most of them are expressed in the male genital tract. Some authors report that adiponectin, in contrast with other adipokines such as resistin or chemerin, has a positive effect on spermatogenesis. Although the pathophysiological role of adipokines in sperm is not yet fully understood, they could influence sperm functionality and could be potential biomarkers of male fertility. High levels of sperm DNA fragmentation have been associated with several adverse reproductive outcomes, although studies have shown conflicting results. Another critical factor in male infertility is oxidative stress, which negatively affects sperm function and viability, also because it triggers DNA alterations, lipid peroxidation and alterations in protein expression, compromising fertilization potential. To better understand the correlation between sperm DNA fragmentation, adiponectin and oxidative stress and their role in clinical practice, we evaluated these parameters in the seminal plasma of males who presented to the infertility study center of Careggi University Hospital of Florence. By accurately evaluating these parameters and their possible correlation, it will be possible to personalize treatment for individual patients.<\/jats:p>","DOI":"10.3390\/antiox14121427","type":"journal-article","created":{"date-parts":[[2025,11,27]],"date-time":"2025-11-27T16:31:52Z","timestamp":1764261112000},"page":"1427","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Association Among Sperm Adiponectin, DNA Fragmentation, Oxidative Stress and Metabolites in Male Infertility"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3333-1618","authenticated-orcid":false,"given":"Rosamaria","family":"Militello","sequence":"first","affiliation":[{"name":"Department of Biomedical, Experimental and Clinical Sciences \u201cMario Serio\u201d, University of Florence, 50134 Florence, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giulia","family":"Traini","sequence":"additional","affiliation":[{"name":"Department of Biomedical, Experimental and Clinical Sciences \u201cMario Serio\u201d, University of Florence, 50134 Florence, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9169-3452","authenticated-orcid":false,"given":"Gabriella","family":"Pinto","sequence":"additional","affiliation":[{"name":"Department of Chemical Sciences, University of Naples Federico II, 80126 Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1489-8934","authenticated-orcid":false,"given":"Tania","family":"Gamberi","sequence":"additional","affiliation":[{"name":"Department of Biomedical, Experimental and Clinical Sciences \u201cMario Serio\u201d, University of Florence, 50134 Florence, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7050-6590","authenticated-orcid":false,"given":"Simone","family":"Luti","sequence":"additional","affiliation":[{"name":"Department of Biomedical, Experimental and Clinical Sciences \u201cMario Serio\u201d, University of Florence, 50134 Florence, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1057-8626","authenticated-orcid":false,"given":"Sara","family":"Marchiani","sequence":"additional","affiliation":[{"name":"Department of Biomedical, Experimental and Clinical Sciences \u201cMario Serio\u201d, University of Florence, 50134 Florence, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1491-8966","authenticated-orcid":false,"given":"Anna","family":"Illiano","sequence":"additional","affiliation":[{"name":"Department of Chemical Sciences, University of Naples Federico II, 80126 Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0907-0630","authenticated-orcid":false,"given":"Linda","family":"Vignozzi","sequence":"additional","affiliation":[{"name":"Department of Biomedical, Experimental and Clinical Sciences \u201cMario Serio\u201d, University of Florence, 50134 Florence, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angela","family":"Amoresano","sequence":"additional","affiliation":[{"name":"Department of Chemical Sciences, University of Naples Federico II, 80126 Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1290-2285","authenticated-orcid":false,"given":"Alessandra","family":"Modesti","sequence":"additional","affiliation":[{"name":"Department of Biomedical, Experimental and Clinical Sciences \u201cMario Serio\u201d, University of Florence, 50134 Florence, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,11,27]]},"reference":[{"key":"ref_1","unstructured":"Leslie, S.W., Soon-Sutton, T.L., and Khan, M.A. (2025). Male Infertility, StatPearls Publishing."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2628","DOI":"10.1021\/acs.jproteome.0c01044","article-title":"Effects of Obesity and Diabetes on Sperm Cell Proteomics in Rats","volume":"20","author":"Carvalho","year":"2021","journal-title":"J. Proteome Res."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Loveland, K.L., Klein, B., Pueschl, D., Indumathy, S., Bergmann, M., Loveland, B.E., Hedger, M.P., and Schuppe, H.-C. (2017). Cytokines in Male Fertility and Reproductive Pathologies: Immunoregulation and Beyond. Front. Endocrinol., 8.","DOI":"10.3389\/fendo.2017.00307"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1007\/s12020-013-0102-0","article-title":"Implications of adiponectin in linking metabolism to testicular function","volume":"46","author":"Martin","year":"2014","journal-title":"Endocrine"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1002\/mrd.23447","article-title":"Follicular Microenvironment: Oxidative Stress and Adiponectin Correlated with Steroids Hormones in Women Undergoing in Vitro Fertilization","volume":"88","author":"Luti","year":"2021","journal-title":"Mol. Reprod. Dev."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1256","DOI":"10.1016\/j.fertnstert.2012.12.022","article-title":"Seminal Plasma Adipokine Levels Are Correlated with Functional Characteristics of Spermatozoa","volume":"99","author":"Thomas","year":"2013","journal-title":"Fertil. Steril."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Stavros, S., Potiris, A., Molopodi, E., Mavrogianni, D., Zikopoulos, A., Louis, K., Karampitsakos, T., Nazou, E., Sioutis, D., and Christodoulaki, C. (2024). Sperm DNA Fragmentation: Unraveling Its Imperative Impact on Male Infertility Based on Recent Evidence. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms251810167"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"e13874","DOI":"10.1111\/and.13874","article-title":"Sperm DNA Fragmentation Testing: Summary Evidence and Clinical Practice Recommendations","volume":"53","author":"Esteves","year":"2021","journal-title":"Andrologia"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Li, F., Duan, X., Li, M., and Ma, X. (2024). Sperm DNA Fragmentation Index Affect Pregnancy Outcomes and Offspring Safety in Assisted Reproductive Technology. Sci. Rep., 14.","DOI":"10.1038\/s41598-023-45091-6"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1035","DOI":"10.1093\/humrep\/den058","article-title":"Nuclear Staining Identifies Two Populations of Human Sperm with Different DNA Fragmentation Extent and Relationship with Semen Parameters","volume":"23","author":"Muratori","year":"2008","journal-title":"Hum. Reprod."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"582","DOI":"10.1016\/j.fertnstert.2015.06.005","article-title":"DNA Fragmentation in Brighter Sperm Predicts Male Fertility Independently from Age and Semen Parameters","volume":"104","author":"Muratori","year":"2015","journal-title":"Fertil. Steril."},{"key":"ref_12","unstructured":"World Health Organization (2021). Sexual and Reproductive Health and Research (SRH) WHO Laboratory Manual for the Examination and Processing of Human Semen, World Health Organization. [6th ed.]."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"e5082","DOI":"10.1002\/jms.5082","article-title":"Molecular Fingerprint by Omics-based Approaches in Saliva from Patients Affected by SARS-CoV-2 Infection","volume":"59","author":"Pinto","year":"2024","journal-title":"J. Mass Spectrom."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"966","DOI":"10.1093\/bioinformatics\/btq054","article-title":"Skyline: An Open Source Document Editor for Creating and Analyzing Targeted Proteomics Experiments","volume":"26","author":"MacLean","year":"2010","journal-title":"Bioinformatics"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"40","DOI":"10.4103\/aja.aja_49_21","article-title":"Evaluation of the Sperm DNA Fragmentation Index in Infertile Japanese Men by In-House Flow Cytometric Analysis","volume":"24","author":"Osaka","year":"2022","journal-title":"Asian J. Androl."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1007\/s43032-020-00408-y","article-title":"Current Insights and Latest Updates in Sperm Motility and Associated Applications in Assisted Reproduction","volume":"29","author":"Dcunha","year":"2022","journal-title":"Reprod. Sci."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Wang, Y., Fu, X., and Li, H. (2025). Mechanisms of Oxidative Stress-Induced Sperm Dysfunction. Front. Endocrinol., 16.","DOI":"10.3389\/fendo.2025.1520835"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1210\/er.2005-0005","article-title":"Adiponectin and Adiponectin Receptors","volume":"26","author":"Kadowaki","year":"2005","journal-title":"Endocr. Rev."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1016\/S1472-6483(10)61174-1","article-title":"Oxidative Stress and Its Implications in Female Infertility\u2014A Clinician\u2019s Perspective","volume":"11","author":"Agarwal","year":"2005","journal-title":"Reprod. Biomed. Online"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1159\/000151396","article-title":"Expression of Adiponectin and Adiponectin Receptors in Human Pituitary Gland and Brain","volume":"89","author":"Psilopanagioti","year":"2009","journal-title":"Neuroendocrinology"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1027","DOI":"10.1016\/j.fertnstert.2009.10.046","article-title":"Sperm DNA Fragmentation: Mechanisms of Origin, Impact on Reproductive Outcome, and Analysis","volume":"93","author":"Sakkas","year":"2010","journal-title":"Fertil. Steril."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"861","DOI":"10.1016\/j.cmet.2015.08.024","article-title":"Histone Methylation Dynamics and Gene Regulation Occur through the Sensing of One-Carbon Metabolism","volume":"22","author":"Mentch","year":"2015","journal-title":"Cell Metab."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Sorgdrager, F.J.H., Naud\u00e9, P.J.W., Kema, I.P., Nollen, E.A., and Deyn, P.P. (2019). De Tryptophan Metabolism in Inflammaging: From Biomarker to Therapeutic Target. Front. Immunol., 10.","DOI":"10.3389\/fimmu.2019.02565"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Zakaria, A., Diawara, I., Bouziyane, A., and Louanjli, N. (2025). Exploring Human Sperm Metabolism and Male Infertility: A Systematic Review of Genomics, Proteomics, Metabolomics, and Imaging Techniques. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26157544"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1530\/REP-12-0145","article-title":"Evidence of 5-HT compo-nents in human sperm: Implications for protein tyrosine phosphorylation and the physiology of motility","volume":"144","author":"Kuhn","year":"2012","journal-title":"Reproduction"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Karunyam, B.V., Abdul Karim, A.K., Naina Mohamed, I., Ugusman, A., Mohamed, W.M.Y., Faizal, A.M., Abu, M.A., and Kumar, J. (2023). Infertility and Cortisol: A Systematic Review. Front. Endocrinol., 14.","DOI":"10.3389\/fendo.2023.1147306"}],"container-title":["Antioxidants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-3921\/14\/12\/1427\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,27]],"date-time":"2025-11-27T16:47:33Z","timestamp":1764262053000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-3921\/14\/12\/1427"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,27]]},"references-count":26,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["antiox14121427"],"URL":"https:\/\/doi.org\/10.3390\/antiox14121427","relation":{},"ISSN":["2076-3921"],"issn-type":[{"value":"2076-3921","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,27]]}}}