{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T01:52:19Z","timestamp":1792979539979,"version":"4.5.0"},"reference-count":42,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T00:00:00Z","timestamp":1769040000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Liangshan Prefecture Science and Technology Bureau","award":["23ZDYF0107"],"award-info":[{"award-number":["23ZDYF0107"]}]},{"name":"Liangshan Prefecture Science and Technology Bureau","award":["24DXWT0001"],"award-info":[{"award-number":["24DXWT0001"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>The main objective of this study was to preliminarily analyze the major flavonoid and phenolic acid components of the ethanolic extract of Gerbera delavayi Franch (E-GDF), and to evaluate its anti-inflammatory and antioxidant properties in lipopolysaccharide (LPS)-stimulated murine macrophage RAW264.7 cells and systemic inflammation mouse models. Results indicated that E-GDF was rich in flavonoids (16.35 \u00b1 0.19 mg RT\/g d.w. Plant Material) and polyphenolic compounds (36.15 \u00b1 0.20 mg GAE\/g d.w. Plant Material). LC-MS analysis of E-GDF revealed that its major flavonoid components included kaempferol glycosides, luteolin, and their glycosylated derivatives, while its phenolic acids were predominantly chlorogenic acid, caffeic acid, ferulic acid, and their corresponding glycosides. E-GDF exhibited good antioxidant activities, including the scavenging of DPPH, ABTS, \u2022OH, and O2\u2022\u2212 radicals. E-GDF treatment significantly inhibited the production of ROS and inflammatory mediators (NO, IL-6, TNF-\u03b1) in LPS-stimulated macrophages (RAW 264.7), while concurrently down-regulating the mRNA expression of COX-2, IL-1\u03b2, Casp1, and GSDMD-1. In addition, in vivo experiments revealed that E-GDF treatment effectively reduced the serum LPS, AST levels, as well as hepatic TNF-\u03b1, IL-6 levels in mice with LPS-induced acute liver injury. Furthermore, E-GDF significantly ameliorated LPS-induced liver pathological damage. These results provide a basis for G. delavayi as a potential antioxidant, anti-inflammatory, and hepatoprotective herbal medicine.<\/jats:p>","DOI":"10.3390\/antiox15010143","type":"journal-article","created":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T10:41:34Z","timestamp":1769164894000},"page":"143","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Phytochemical Composition, Antioxidant and Anti-Inflammatory Activities, and Protective Effect Against LPS-Induced Liver Injury in Mice of Gerbera delavayi Franch"],"prefix":"10.3390","volume":"15","author":[{"given":"Hongmei","family":"Yin","sequence":"first","affiliation":[{"name":"College of Animal Science, Xichang University, Xichang 615000, China"},{"name":"College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yinrong","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rouxian","family":"Hu","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanhang","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huaqiao","family":"Tang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyan","family":"Li","sequence":"additional","affiliation":[{"name":"College of Animal Science, Xichang University, Xichang 615000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gang","family":"Ye","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Shi","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng","family":"Lv","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6505-5270","authenticated-orcid":false,"given":"Ling","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,1,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1111\/j.1365-2249.2006.03261.x","article-title":"Chronic inflammation: Importance of NOD2 and NALP3 in interleukin-1beta generation","volume":"147","author":"Nielsen","year":"2007","journal-title":"Clin. Exp. Immunol."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Kim, K.M., Kim, S.Y., Mony, T.J., Bae, H.J., Han, S.D., Lee, E.S., Choi, S.H., Hong, S.H., Lee, S.D., and Park, S.J. (2021). Dracocephalum moldavica Ethanol Extract Suppresses LPS-Induced Inflammatory Responses through Inhibition of the JNK\/ERK\/NF-\u03baB Signaling Pathway and IL-6 Production in RAW 264.7 Macrophages and in Endotoxic-Treated Mice. Nutrients, 13.","DOI":"10.3390\/nu13124501"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1038\/s41572-019-0105-0","article-title":"Drug-induced liver injury","volume":"5","author":"Andrade","year":"2019","journal-title":"Nat. Rev. Dis. Primers"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"5975","DOI":"10.1038\/s41598-024-80903-3","article-title":"Clusterin inhibits lipopolysaccharide induced liver injury","volume":"15","author":"Seo","year":"2025","journal-title":"Sci. Rep."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Xu, D., Xu, M., Jeong, S., Qian, Y., Wu, H., Xia, Q., and Kong, X. (2018). The Role of Nrf2 in Liver Disease: Novel Molecular Mechanisms and Therapeutic Approaches. Front. Pharmacol., 9.","DOI":"10.3389\/fphar.2018.01428"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"4088","DOI":"10.1007\/s10924-021-02179-2","article-title":"Platycodon grandiflorus Polysaccharide with Anti-Apoptosis, Anti-Oxidant and Anti-Inflammatory Activity Against LPS\/D-GalN Induced Acute Liver Injury in Mice","volume":"29","author":"Qi","year":"2021","journal-title":"J. Polym. Environ."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"101239","DOI":"10.1016\/j.redox.2019.101239","article-title":"Programmed necrotic cell death of macrophages: Focus on pyroptosis, necroptosis, and parthanatos","volume":"26","author":"Robinson","year":"2019","journal-title":"Redox Biol."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Lee, S.C., Ahn, J., Kim, J., Lee, J.Y., Kim, J., Uddin, M.S., Lee, S.W., and Kim, C.Y. (2023). The Antioxidant and Anti-Inflammatory Properties of Merremia umbellata Extract. Antioxidants, 12.","DOI":"10.3390\/antiox12122037"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"105568","DOI":"10.1016\/j.fitote.2023.105568","article-title":"New 5-methyl-4-hydroxycoumarin polyketide derivatives from Gerbera delavayi with anti-inflammatory activity","volume":"169","author":"Yang","year":"2023","journal-title":"Fitoterapia"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"548","DOI":"10.1111\/tpj.13216","article-title":"Two polyketide synthases are necessary for 4-hydroxy-5-methylcoumarin biosynthesis in Gerbera hybrida","volume":"87","author":"Kontturi","year":"2016","journal-title":"Plant J. Cell Mol. Biol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"105258","DOI":"10.1016\/j.fitote.2022.105258","article-title":"Bioactive constituents from Gerbera piloselloides with anti-inflammatory and antiproliferative activities","volume":"161","author":"Zhao","year":"2022","journal-title":"Fitoterapia"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"114296","DOI":"10.1016\/j.phytochem.2024.114296","article-title":"LC-MS coupled with diagnostic ion strategy facilitated the discovery of 5-methylcoumarin meroterpenoids from Gerbera piloselloides","volume":"229","author":"Chen","year":"2025","journal-title":"Phytochemistry"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"3213","DOI":"10.1007\/s10787-025-01720-2","article-title":"Phytochemical studies of Gerbera jamesonii and evaluation of anti-inflammatory potential in formaldehyde-induced arthritis in rats","volume":"33","author":"Ullah","year":"2025","journal-title":"Inflammopharmacology"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"117852","DOI":"10.1016\/j.jep.2024.117852","article-title":"Integration of metabolomics and transcriptomics to reveal the mechanism of Gerberae piloselloidis herba in alleviating bronchial asthma","volume":"325","author":"Liu","year":"2024","journal-title":"J. Ethnopharmacol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"115624","DOI":"10.1016\/j.jep.2022.115624","article-title":"Integrated plasma pharmacochemistry and network pharmacology to explore the mechanism of Gerberae piloselloidis Herba in treatment of allergic asthma","volume":"298","author":"Zhou","year":"2022","journal-title":"J. Ethnopharmacol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2657","DOI":"10.1016\/j.apsb.2024.03.035","article-title":"(+)\/(\u2212)-Gerbeloid A, a pair of unprecedented coumarin-based polycyclic meroterpenoid enantiomers from Gerbera piloselloides: Structural elucidation, semi-synthesis, and lipid-lowering activity","volume":"14","author":"Zhao","year":"2024","journal-title":"Acta Pharm. Sin. B"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"104299","DOI":"10.1016\/j.fitote.2019.104299","article-title":"8-methoxysmyrindiol from Gerbera piloselloides (L.) Cass. and its vasodilation effects on isolated rat mesenteric arteries","volume":"138","author":"He","year":"2019","journal-title":"Fitoterapia"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"4046","DOI":"10.3390\/molecules19044046","article-title":"Chemical Composition and Biological Activities of Gerbera anandria","volume":"19","author":"He","year":"2014","journal-title":"Molecules"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Chera, E.I., Pop, R.M., P\u00e2rvu, M., Sori\u021b\u0103u, O., Uif\u0103lean, A., C\u0103toi, F.A., Cecan, A., Negoescu, A.G., Achima\u0219-Cadariu, P., and P\u00e2rvu, A.E. (2022). Flaxseed Ethanol Extracts\u2019 Antitumor, Antioxidant, and Anti-Inflammatory Potential. Antioxidants, 11.","DOI":"10.3390\/antiox11050892"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"105917","DOI":"10.1016\/j.ultsonch.2022.105917","article-title":"Antioxidant and anti-inflammatory polyphenols in ultrasound-assisted extracts from salvilla (Buddleja scordioides Kunth)","volume":"83","year":"2022","journal-title":"Ultrason. Sonochemistry"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"118149","DOI":"10.1016\/j.carbpol.2021.118149","article-title":"Structural characterization and antioxidant activities of Bletilla striata polysaccharide extracted by different methods","volume":"266","author":"Chen","year":"2021","journal-title":"Carbohydr. Polym."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1016\/j.talanta.2018.11.044","article-title":"A novel strategy for colorimetric detection of hydroxyl radicals based on a modified Griess test","volume":"195","author":"Deng","year":"2019","journal-title":"Talanta"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1016\/S0024-3205(97)00004-0","article-title":"Free radical scavenging activities of mushroom polysaccharide extracts","volume":"60","author":"Liu","year":"1997","journal-title":"Life Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"106584","DOI":"10.1016\/j.fitote.2025.106584","article-title":"Leontopodium species: Phytochemistry, biosynthesis, synthesis, pharmacology, and synthetic advancement","volume":"183","author":"Hanh","year":"2025","journal-title":"Fitoterapia"},{"key":"ref_25","first-page":"422","article-title":"Chemical constituents of Leontopodium leontopodioid (wild.) Beauv","volume":"6","author":"Li","year":"2008","journal-title":"Cent. South Pharm."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1007\/s11418-017-1132-3","article-title":"Bioactive constituents from the whole plants of Leontopodium leontopodioides (Wild.) Beauv","volume":"72","author":"Chen","year":"2018","journal-title":"J. Nat. Med."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"116445","DOI":"10.1016\/j.ejmech.2024.116445","article-title":"Sesquilignans: Current research and potential prospective","volume":"271","author":"Wu","year":"2024","journal-title":"Eur. J. Med. Chem."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1379","DOI":"10.1016\/j.phytochem.2011.04.006","article-title":"Metabolic fingerprinting of Leontopodium species (Asteraceae) by means of 1H NMR and HPLC-ESI-MS","volume":"72","author":"Safer","year":"2011","journal-title":"Phytochemistry"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Olech, M., \u0141yko, L., and Nowak, R. (2020). Influence of Accelerated Solvent Extraction Conditions on the LC-ESI-MS\/MS Polyphenolic Profile, Triterpenoid Content, and Antioxidant and Anti-lipoxygenase Activity of Rhododendron luteum Sweet Leaves. Antioxidants, 9.","DOI":"10.3390\/antiox9090822"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"2523","DOI":"10.1016\/S0031-9422(00)84090-X","article-title":"High pressure liquid chromatographic analysis of flavonoid chemical markers in petals from Gerbera flowers as an adjunct for cultivar and germplasm identification","volume":"23","author":"Asen","year":"1984","journal-title":"Phytochemistry"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"140949","DOI":"10.1016\/j.foodchem.2024.140949","article-title":"Obtaining and characterization of natural extracts from mango (Mangifera indica) peel and its effect on the rheological behavior in new mango kernel starch hydrogels","volume":"462","year":"2025","journal-title":"Food Chem."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/j.foodchem.2019.04.105","article-title":"Evaluation of cellular antioxidant components of honeys using UPLC-MS\/MS and HPLC-FLD based on the quantitative composition-activity relationship","volume":"293","author":"Shen","year":"2019","journal-title":"Food Chem."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1016\/j.carbpol.2017.04.076","article-title":"Enhanced solubility and antioxidant activity of chlorogenic acid-chitosan conjugates due to the conjugation of chitosan with chlorogenic acid","volume":"170","author":"Rui","year":"2017","journal-title":"Carbohydr. Polym."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1245","DOI":"10.1039\/C6FO01735E","article-title":"Molecular mechanisms of the effects of the ethanolic extract of Muntingia calabura Linn. fruit on lipopolysaccharide-induced pro-inflammatory mediators in macrophages","volume":"8","author":"Lin","year":"2017","journal-title":"Food Funct."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"675","DOI":"10.5246\/jcps.2021.08.055","article-title":"Anti-inflammatory effects of diaporisoindole B in LPS-stimulated RAW 264.7 macrophage cells via MyD88 activated NF-\u03baB and MAPKs pathways","volume":"30","author":"Liu","year":"2021","journal-title":"J. Chin. Pharm. Sci."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1111\/imr.12621","article-title":"Overview of the IL-1 family in innate inflammation and acquired immunity","volume":"281","author":"Dinarello","year":"2018","journal-title":"Immunol. Rev."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Zhang, B., Fan, C., Tan, Q., Zhang, Y., Jiang, Q., Yu, Q., Zhang, B., Zheng, K., and Yan, C. (2022). rCsHscB Derived from Clonorchis sinensis: A Carcinogenic Liver Fluke Ameliorates LPS-Induced Acute Hepatic Injury by Repression of Inflammation. Pathogens, 11.","DOI":"10.3390\/pathogens11121548"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1610","DOI":"10.1038\/s41401-020-00597-x","article-title":"Deletion of TLR4 attenuates lipopolysaccharide-induced acute liver injury by inhibiting inflammation and apoptosis","volume":"42","author":"Chen","year":"2021","journal-title":"Acta Pharmacol. Sin."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/j.intimp.2018.12.059","article-title":"Hepatoprotective effects and structure-activity relationship of five flavonoids against lipopolysaccharide\/d-galactosamine induced acute liver failure in mice","volume":"68","author":"He","year":"2019","journal-title":"Int. Immunopharmacol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"871","DOI":"10.1007\/s00011-010-0199-z","article-title":"Protective effects of chlorogenic acid on acute hepatotoxicity induced by lipopolysaccharide in mice","volume":"59","author":"Xu","year":"2010","journal-title":"Inflamm. Res."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1038\/nrgastro.2016.168","article-title":"Liver\u2014Guardian, modifier and target of sepsis","volume":"14","author":"Strnad","year":"2017","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"111670","DOI":"10.1016\/j.biopha.2021.111670","article-title":"Captopril downregulates expression of Bax\/cytochrome C\/caspase-3 apoptotic pathway, reduces inflammation, and oxidative stress in cisplatin-induced acute hepatic injury","volume":"139","author":"Eid","year":"2021","journal-title":"Biomed Pharmacother."}],"container-title":["Antioxidants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-3921\/15\/1\/143\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T11:00:51Z","timestamp":1769166051000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-3921\/15\/1\/143"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,22]]},"references-count":42,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2026,1]]}},"alternative-id":["antiox15010143"],"URL":"https:\/\/doi.org\/10.3390\/antiox15010143","relation":{},"ISSN":["2076-3921"],"issn-type":[{"value":"2076-3921","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,22]]}}}