{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T12:33:12Z","timestamp":1772195592257,"version":"3.50.1"},"reference-count":41,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T00:00:00Z","timestamp":1772150400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Minister of Science under \u201cthe Regional Initiative of Excellence Program\u201d"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>Myo-inositol, the most common stereoisomer of inositol, plays an important role in many physiological processes, such as cell signaling, regulation of glucose and lipid metabolism, and protection of cells against oxidative stress. The main focus has been on pharmacokinetics, and it has been studied in both animal models (Wistar rats, mice, and Danio rerio) and humans. It is characterized by high oral bioavailability and is primarily eliminated via the kidneys. Preclinical studies have shown that myo-inositol has hepatoprotective potential, reducing oxidative stress, inflammation, and lipid accumulation in hepatocytes, as well as stabilizing liver cell membranes. Animal models make it possible to assess mechanisms of action, toxicity, and efficacy, thereby laying the groundwork for clinical research. In clinical practice, myo-inositol is currently used mainly in the treatment of polycystic ovary syndrome, gestational diabetes, fertility disorders, and certain affective disorders. Based on the results of preclinical studies, its potential application in liver diseases and drug-induced injury has been suggested. Despite promising findings, further translational research and randomized clinical trials are necessary to evaluate the therapeutic efficacy and safety of myo-inositol in hepatology. In summary, myo-inositol is a natural, well-tolerated compound with a multidirectional mechanism of action that may represent a promising element of supportive therapy for liver diseases.<\/jats:p>","DOI":"10.3390\/antiox15030297","type":"journal-article","created":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T10:41:02Z","timestamp":1772188862000},"page":"297","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Myo-Inositol: Pharmacokinetics, Biological Functions, and Therapeutic Potential in Liver Protection: Insights from Preclinical Models"],"prefix":"10.3390","volume":"15","author":[{"given":"Tomasz","family":"Antonowski","sequence":"first","affiliation":[{"name":"Department of Human Physiology and Pathophysiology, School of Medicine, Collegium Medicum, University of Warmia and Mazury in Olsztyn, 10-085 Olsztyn, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0544-5653","authenticated-orcid":false,"given":"Adam","family":"Osowski","sequence":"additional","affiliation":[{"name":"Department of Human Physiology and Pathophysiology, School of Medicine, Collegium Medicum, University of Warmia and Mazury in Olsztyn, 10-085 Olsztyn, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0856-3644","authenticated-orcid":false,"given":"Joanna","family":"Wojtkiewicz","sequence":"additional","affiliation":[{"name":"Department of Human Physiology and Pathophysiology, School of Medicine, Collegium Medicum, University of Warmia and Mazury in Olsztyn, 10-085 Olsztyn, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,2,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"110012","DOI":"10.1016\/j.jnutbio.2025.110012","article-title":"Myo-inositol supplementation in diet-induced obese lactating rats mitigates metabolic dysregulation and improves offspring health in early life","volume":"145","author":"Valle","year":"2025","journal-title":"J. Nutr. Biochem."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1159\/000536081","article-title":"d-Chiro-Inositol in Clinical Practice: A Perspective from the Experts Group on Inositol in Basic and Clinical Research (EGOI)","volume":"89","author":"Dinicola","year":"2024","journal-title":"Gynecol. Obstet. Investig."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Wojciechowska, A.M., Zaj\u0105c, P., Gogola-Mruk, J., Kowalik, M.K., and Ptak, A. (2024). Myo-Inositol and D-Chiro-Inositol Reduce DHT-Stimulated Changes in the Steroidogenic Activity of Adult Granulosa Cell Tumors. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms252010974"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1301","DOI":"10.1016\/j.chom.2024.06.012","article-title":"Obesity-enriched gut microbe degrades myo-inositol and promotes lipid absorption","volume":"32","author":"Wu","year":"2024","journal-title":"Cell Host Microbe"},{"key":"ref_5","first-page":"893","article-title":"Myo-inositol: Its metabolism and potential implications for poultry nutrition\u2014A review","volume":"99","author":"Rodehutscord","year":"2019","journal-title":"Poult. Sci."},{"key":"ref_6","first-page":"2390","article-title":"From Myo-inositol to D-chiro-inositol molecular pathways","volume":"25","author":"Kiani","year":"2021","journal-title":"Eur. Rev. Med. Pharmacol. Sci."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Antonowski, T., Wi\u015bniewski, K., Podlasz, P., Osowski, A., and Wojtkiewicz, J. (2021). Study of the Potential Hepatoprotective Effect of Myo-Inositol and Its Influence on Zebrafish Development. Nutrients, 13.","DOI":"10.3390\/nu13103346"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Antonowski, T., Osowski, A., Szczesny, D., Szabli\u0144ska-Piernik, J., Ju\u015bkiewicz, J., Lahuta, L., Rynkiewicz, A., and Wojtkiewicz, J. (2022). Pharmacokinetics of Myo-Inositol in a Wistar Rat Animal Model. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms231911246"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Wi\u015bniewski, K., Zglejc-Waszak, K., Antonowski, T., Szablinska-Piernik, J., Juskiewicz, J., Lahuta, L., Jozwik, M., and Wojtkiewicz, J. (2025). Analysis of Scyllo-Inositol in a Wistar Rat Animal Model\u2014A Preliminary Study. Pharmaceuticals, 18.","DOI":"10.3390\/ph18070954"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Bizzarri, M., Monti, N., Piombarolo, A., Angeloni, A., and Verna, R. (2023). Myo-Inositol and D-Chiro-Inositol as Modulators of Ovary Steroidogenesis: A Narrative Review. Nutrients, 15.","DOI":"10.3390\/nu15081875"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2301554","DOI":"10.1080\/09513590.2023.2301554","article-title":"Update on the combination of myo-inositol\/d-chiro-inositol for the treatment of polycystic ovary syndrome","volume":"40","author":"Lete","year":"2024","journal-title":"Gynecol. Endocrinol."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Tu-Sekine, B., and Kim, S.F. (2022). The Inositol Phosphate System\u2014A Coordinator of Metabolic Adaptability. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23126747"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1098\/rstb.1988.0077","article-title":"Inositol phosphates: Proliferation, metabolism and function","volume":"320","author":"Irvine","year":"1988","journal-title":"Philos. Trans. R. Soc. Lond. B Biol. Sci."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Chatree, S., Thongmaen, N., Tantivejkul, K., Sitticharoon, C., and Vucenik, I. (2020). Role of Inositols and Inositol Phosphates in Energy Metabolism. Molecules, 25.","DOI":"10.3390\/molecules25215079"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"wraf161","DOI":"10.1093\/ismejo\/wraf161","article-title":"Inositol phosphates as an overlooked phosphorous source in marine ecosystems","volume":"19","author":"Teng","year":"2025","journal-title":"ISME J."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1007\/s12640-025-00735-5","article-title":"Protective Effect of myo-Inositol Against Decitabine-Induced Neural Tube Defects in Embryonic Zebrafish","volume":"43","author":"Rajesh","year":"2025","journal-title":"Neurotox. Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"105047","DOI":"10.1016\/j.phrs.2020.105047","article-title":"Myo\u2013inositol and d-chiro\u2013inositol oral supplementation ameliorate cardiac dysfunction and remodeling in a mouse model of diet-induced obesity","volume":"159","author":"Nicolini","year":"2020","journal-title":"Pharmacol. Res."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"L\u00f3pez-Gambero, A.J., Sanjuan, C., Serrano-Castro, P.J., Su\u00e1rez, J., and Rodr\u00edguez de Fonseca, F. (2020). The Biomedical Uses of Inositols: A Nutraceutical Approach to Metabolic Dysfunction in Aging and Neurodegenerative Diseases. Biomedicines, 8.","DOI":"10.3390\/biomedicines8090295"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1630","DOI":"10.1210\/clinem\/dgad762","article-title":"Inositol for Polycystic Ovary Syndrome: A Systematic Review and Meta-analysis to Inform the 2023 Update of the International Evidence-based PCOS Guidelines","volume":"109","author":"Fitz","year":"2024","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_20","first-page":"438","article-title":"The use of D-chiro-Inositol in clinical practice","volume":"25","author":"Gambioli","year":"2021","journal-title":"Eur. Rev. Med. Pharmacol. Sci."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Kamel, F.O., Karim, S., Bafail, D.A.O., Aldawsari, H.M., Kotta, S., and Ilyas, U.K. (2023). Hepatoprotective effects of bioactive compounds from traditional herb Tulsi (Ocimum sanctum Linn) against galactosamine-induced hepatotoxicity in rats. Front. Pharmacol., 14.","DOI":"10.3389\/fphar.2023.1213052"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1016\/j.jtcme.2020.03.005","article-title":"Inositols and metabolic disorders: From farm to bedside","volume":"10","author":"Caputo","year":"2020","journal-title":"J. Tradit. Complement. Med."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1335","DOI":"10.1097\/HEP.0000000000000004","article-title":"The global epidemiology of nonalcoholic fatty liver disease (NAFLD) and nonalcoholic steatohepatitis (NASH): A systematic review","volume":"77","author":"Younossi","year":"2023","journal-title":"Hepatology"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Lepore, E., Lauretta, R., Bianchini, M., Mormando, M., Di Lorenzo, C., and Unfer, V. (2021). Inositols Depletion and Resistance: Principal Mechanisms and Therapeutic Strategies. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22136796"},{"key":"ref_25","first-page":"5483","article-title":"Combined treatment of myo-inositol and d-chiro-inositol (80:1) as a therapeutic approach to restore inositol eumetabolism in patients with bipolar disorder taking lithium and valproic acid","volume":"25","author":"Janiri","year":"2021","journal-title":"Eur. Rev. Med. Pharmacol. Sci."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Zhu, J., Pan, J., Wang, X., Huang, Y., Qin, C., Qiao, F., Qin, J., and Chen, L. (2020). Alleviation of the Adverse Effect of Dietary Carbohydrate by Supplementation of Myo-Inositol to the Diet of Nile Tilapia (Oreochromis niloticus). Animals, 10.","DOI":"10.3390\/ani10112190"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"25633","DOI":"10.1038\/s41598-024-76527-2","article-title":"Antioxidant and anticoagulant properties of myo-inositol determined in an ex vivo studies and gas chromatography analysis","volume":"14","author":"Rolnik","year":"2024","journal-title":"Sci. Rep."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Shimizu, N., Shiraishi, H., and Hanada, T. (2023). Zebrafish as a Useful Model System for Human Liver Disease. Cells, 12.","DOI":"10.3390\/cells12182246"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Castillo, P., Palou, M., Yau-Qiu, Z.X., Rodr\u00edguez, A.M., Palou, A., and Pic\u00f3, C. (2021). Myo-Inositol Supplementation in Suckling Rats Protects against Adverse Programming Outcomeson Hypothalamic Structure Caused by Mild Gestational Calorie Restriction, Partially Comparable to Leptin Effects. Nutrients, 13.","DOI":"10.3390\/nu13093257"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"203","DOI":"10.17221\/116\/2024-VETMED","article-title":"Antioxidant and cytokine modulation in PCOS rats protected with probiotics, myo-inositol, and herbal extracts","volume":"70","author":"Zaki","year":"2025","journal-title":"Vet. Med."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"117287","DOI":"10.1016\/j.biopha.2024.117287","article-title":"Oral supplementation of inositols effectively recovered lithium-induced cardiac dysfunctions in mice","volume":"178","author":"Nicolini","year":"2024","journal-title":"Biomed. Pharmacother."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"100921","DOI":"10.1016\/j.jbior.2022.100921","article-title":"Regulations of myo-inositol homeostasis: Mechanisms, implications, and perspectives","volume":"87","author":"Su","year":"2023","journal-title":"Adv. Biol. Regul."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Chhetri, D.R. (2019). Myo-Inositol and Its Derivatives: Their Emerging Role in the Treatment of Human Diseases. Front. Pharmacol., 10.","DOI":"10.3389\/fphar.2019.01172"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Jawad, A., Oh, D., Choi, H., Kim, M., Ham, J., Oh, B.C., Lee, J., and Hyun, S.H. (2024). Myo-inositol improves developmental competence and reduces oxidative stress in porcine parthenogenetic embryos. Front. Vet. Sci., 11.","DOI":"10.3389\/fvets.2024.1475329"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Arefhosseini, S., Roshanravan, N., Tutunchi, H., Rostami, S., Khoshbaten, M., and Ebrahimi-Mameghani, M. (2023). Myo-inositol supplementation improves cardiometabolic factors, anthropometric measures, and liver function in obese patients with non-alcoholic fatty liver disease. Front. Nutr., 10.","DOI":"10.3389\/fnut.2023.1092544"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"skac187","DOI":"10.1093\/jas\/skac187","article-title":"Effects of supplemental myo-inositol on growth performance and apparent total tract digestibility of weanling piglets fed reduced protein high-phytate diets and intestinal epithelial cell proliferation and function","volume":"100","author":"Ogunribido","year":"2022","journal-title":"J. Anim. Sci."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Pani, A., Giossi, R., Menichelli, D., Fittipaldo, V.A., Agnelli, F., Inglese, E., Romandini, A., Roncato, R., Pintaudi, B., and Del Sole, F. (2020). Inositol and Non-Alcoholic Fatty Liver Disease: A Systematic Review on Deficiencies and Supplementation. Nutrients, 12.","DOI":"10.3390\/nu12113379"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Mo, D., Lv, M., and Mao, X. (2024). Using different zebrafish models to explore liver regeneration. Front. Cell Dev. Biol., 12.","DOI":"10.3389\/fcell.2024.1485773"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1517\/17425247.2012.662953","article-title":"Myo-inositol in a new pharmaceutical form: A step forward to a broader clinical use","volume":"9","author":"Carlomagno","year":"2012","journal-title":"Expert Opin. Drug Deliv."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Fedeli, V., Catizone, A., Querqui, A., Unfer, V., and Bizzarri, M. (2023). The Role of Inositols in the Hyperandrogenic Phenotypes of PCOS: A Re-Reading of Larner\u2019s Results. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24076296"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Dinicola, S., Unfer, V., Facchinetti, F., Soulage, C.O., Greene, N.D., Bizzarri, M., Lagan\u00e0, A.S., Chan, S.Y., Bevilacqua, A., and Pkhaladze, L. (2021). Inositols: From Established Knowledge to Novel Approaches. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms221910575"}],"container-title":["Antioxidants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-3921\/15\/3\/297\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T11:05:56Z","timestamp":1772190356000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-3921\/15\/3\/297"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,27]]},"references-count":41,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2026,3]]}},"alternative-id":["antiox15030297"],"URL":"https:\/\/doi.org\/10.3390\/antiox15030297","relation":{},"ISSN":["2076-3921"],"issn-type":[{"value":"2076-3921","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,27]]}}}