{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:33:15Z","timestamp":1787027595912,"version":"build-2736575974"},"reference-count":46,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T00:00:00Z","timestamp":1774915200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Directorate of Research and Community Service, Directorate General of Research and Development, Ministry of Higher Education, Science, and Technology, Republic of Indonesia","award":["10.69\/UN23.34\/PT.01\/VI\/2025"],"award-info":[{"award-number":["10.69\/UN23.34\/PT.01\/VI\/2025"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>Sargassum is a widespread brown seaweed species and a source of bioactive compounds with promising antioxidant potential. Unfortunately, to date, the Sargassum species remains largely unexplored. This study was conducted to explore the bioactive compounds from Sargassum ilicifolium extracts collected from Nguyahan and Sundak Beaches, Gunungkidul, Indonesia, by observing Liquid Chromatography\u2013High Resolution Mass Spectrometry (LC-HRMS)-based metabolomics profiling and antioxidant activity assays. Metabolomic analysis detected 506 molecular features across different extraction methods and solvents, with five metabolites putatively dereplicated, including atractylenolide III, pheophorbide A, 13-docosenamide, 1,3,6,8-tetrahydroxy-2-(1-hydroxyhexyl)anthracene-9,10-dione, and 5-hydroxy-6E,8Z,11Z,14Z,17Z-eicosapentaenoic acid. Extraction parameters, particularly solvent polarity and sample pretreatment, have been shown to affect the metabolite variation. Dried samples showed less variation in metabolites than the fresh sample. Antioxidant activity assay showed a moderate to high radical scavenging activity (30\u2013100%), with methanol extracts as a polar solvent inhibited more than semipolar solvents. This study provides a metabolomics-guided assessment of the antioxidant potential of S. ilicifolium, supporting its value and potential as a source of bioactive compounds for future pharmaceutical and nutraceutical applications.<\/jats:p>","DOI":"10.3390\/antiox15040433","type":"journal-article","created":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T10:09:21Z","timestamp":1775038161000},"page":"433","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["LC-HRMS-Based Metabolomic Profiling and Antioxidant Activity of Sargassum ilicifolium Under Different Pretreatments, Extraction Methods, and Solvents"],"prefix":"10.3390","volume":"15","author":[{"given":"Anita Dilla","family":"Harfiyani","sequence":"first","affiliation":[{"name":"Magister of Agricultural Biotechnology Study Program, Graduate School, Universitas Jenderal Soedirman, Purwokerto 53122, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0665-8092","authenticated-orcid":false,"family":"Riyanti","sequence":"additional","affiliation":[{"name":"Magister of Agricultural Biotechnology Study Program, Graduate School, Universitas Jenderal Soedirman, Purwokerto 53122, Indonesia"},{"name":"Faculty of Fisheries and Marine Sciences, Universitas Jenderal Soedirman, Purwokerto 53122, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Nursid","sequence":"additional","affiliation":[{"name":"Research Center for Food Technology and Processing, National Research and Innovation Agency (BRIN), Playen, Yogyakarta 55861, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9947-8079","authenticated-orcid":false,"given":"Till F.","family":"Sch\u00e4berle","sequence":"additional","affiliation":[{"name":"Faculty of Agricultural Sciences, Nutritional Sciences and Environmental Management, Justus Liebig University, 35390 Giessen, Germany"},{"name":"Natural Product Department, Fraunhofer-Institute for Molecular Biology and Applied Ecology (IME), Ohlebergsweg 12, 35392 Giessen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Alexandra","family":"Patras","sequence":"additional","affiliation":[{"name":"Faculty of Agricultural Sciences, Nutritional Sciences and Environmental Management, Justus Liebig University, 35390 Giessen, Germany"},{"name":"Natural Product Department, Fraunhofer-Institute for Molecular Biology and Applied Ecology (IME), Ohlebergsweg 12, 35392 Giessen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8151-6054","authenticated-orcid":false,"given":"Jae-Suk","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Seafood Science and Technology, The Institute of Marine Industry, Gyeongsang National University, 38 Cheondaegukchi-gil, Tongyeong-si 53064, Gyeongsangnam-do, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6334-4172","authenticated-orcid":false,"given":"Maria Dyah Nur","family":"Meinita","sequence":"additional","affiliation":[{"name":"Magister of Agricultural Biotechnology Study Program, Graduate School, Universitas Jenderal Soedirman, Purwokerto 53122, Indonesia"},{"name":"Faculty of Fisheries and Marine Sciences, Universitas Jenderal Soedirman, Purwokerto 53122, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,3,31]]},"reference":[{"key":"ref_1","unstructured":"Food and Agriculture Organization (FAO) (2024). 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