{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T02:47:44Z","timestamp":1792810064978,"version":"4.5.0"},"reference-count":36,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T00:00:00Z","timestamp":1777334400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Science and Higher Education of the Republic of Kazakhstan","award":["AP23489874"],"award-info":[{"award-number":["AP23489874"]}]},{"award":["AP23489874"],"award-info":[{"award-number":["AP23489874"]}],"id":[{"id":"https:\/\/ror.org\/05tyne317","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>Background: This study proposes a cascade strategy for the comprehensive valorization of Rosa canina L. leaves, considered an underutilized agricultural by-product. Methods: The approach is based on a combination of optimized Ultrasound-assisted extraction (UAE) followed by High-pressure homogenization (HPH) of the residual biomass from both whole and ground leaves. UAE parameters (temperature, process duration, and ethanol concentration) were optimized to maximize the yield of total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activity (DPPH, FRAP). Results: The optimal conditions (55.5 \u00b0C, 69.7 min, 40.8% ethanol) yielded extracts with a high TPC (289.55 mg GAE\/g) and TFC (177.88 mg CE\/g), reducing the processing time by 22% while increasing the TPC yield by 31% compared to the conventional solid\u2013liquid extraction (SLE). It was found that primary extraction from whole leaves is more efficient than extraction from ground leaves, suggesting that the energy-intensive preliminary grinding step could be eliminated. The application of HPH to the residual biomass provided a significant secondary release of bioactive compounds, exceeding high-shear mixing (HSM) by up to 1.5 times for whole leaves. Kinetic analysis showed a higher release of bioactive compounds from whole leaves compared to ground leaves. Conclusions: The proposed UAE + HPH cascade process is a sustainable approach, ensuring rational use of resources and a significant increase in the total yield of antioxidants from Rosa canina L. leaves. Overall, the study may contribute to the circular economy by promoting valorization of agricultural by-products through an energy-efficient, sustainable cascade approach.<\/jats:p>","DOI":"10.3390\/antiox15050560","type":"journal-article","created":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T09:46:11Z","timestamp":1777455971000},"page":"560","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Enhanced Recovery of Bioactive Compounds from Rosa canina L. Leaves: A Cascade Approach Using Ultrasounds and High-Pressure Homogenization"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7258-746X","authenticated-orcid":false,"given":"Zhanar","family":"Nabiyeva","sequence":"first","affiliation":[{"name":"Research Institute of Food Safety, Almaty Technological University, Almaty 050012, Kazakhstan"},{"name":"Department of Applied Biotechnology, Almaty Technological University, Almaty 050012, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0254-8307","authenticated-orcid":false,"given":"Serena","family":"Carpentieri","sequence":"additional","affiliation":[{"name":"Department of Industrial Engineering, University of Salerno, 84084 Fisciano, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3878-8185","authenticated-orcid":false,"given":"Akerke","family":"Kulaipbekova","sequence":"additional","affiliation":[{"name":"Department of Applied Biotechnology, Almaty Technological University, Almaty 050012, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5286-5175","authenticated-orcid":false,"given":"Abdyssemat","family":"Samadun","sequence":"additional","affiliation":[{"name":"Research Institute of Food Safety, Almaty Technological University, Almaty 050012, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0395-3379","authenticated-orcid":false,"given":"Yuliya","family":"Pronina","sequence":"additional","affiliation":[{"name":"Department of Food Technology, Almaty Technological University, Almaty 050012, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7964-7736","authenticated-orcid":false,"given":"Elmira","family":"Assembayeva","sequence":"additional","affiliation":[{"name":"Department of Applied Biotechnology, Almaty Technological University, Almaty 050012, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanna","family":"Ferrari","sequence":"additional","affiliation":[{"name":"Department of Industrial Engineering, University of Salerno, 84084 Fisciano, Italy"},{"name":"ProdAl Scarl, University of Salerno, 84084 Fisciano, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,4,28]]},"reference":[{"key":"ref_1","first-page":"100029","article-title":"The circular bioeconomy: Its elements and role in European bioeconomy clusters","volume":"6","author":"Stegmann","year":"2020","journal-title":"Resour. 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