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Mouse embryos deficient in Scavenger Receptor Class B Type 1 (SR-B1 KO), the main receptor for high-density lipoproteins, exhibit a high incidence of anterior NTD, which is associated with vitamin E deficiency and elevated levels of reactive oxygen species (ROS). Maternal supplementation with vitamin E, a micronutrient with antioxidant properties, completely prevents the occurrence of NTD and normalizes ROS levels in SR-B1 KO embryos, suggesting a contribution of oxidative stress to NTD in this model. In this work, we showed that SR-B1 KO embryos at gestational day E9.5 display higher levels of lipoperoxidative damage markers. Analysis of data obtained through shotgun lipidomics evidenced a selective and coordinated reorganization of fatty acid distribution, characterized by altered polyunsaturated and monounsaturated composition, together with reduced phosphatidylcholine and increased lysophosphatidylcholine levels, and diversion of fatty acids into triacylglyceride storage. Transcriptomic analysis revealed a coordinated upregulation of genes involved in phospholipid synthesis and remodeling, consistent with the altered lipid homeostasis observed in SR-B1 KO embryos. Together, these results provide novel information showing a potential link between oxidative stress and disruptions in mammalian embryonic lipid metabolism, highlighting phospholipid remodeling as a potential determinant of susceptibility to NTD.<\/jats:p>","DOI":"10.3390\/antiox15050634","type":"journal-article","created":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T15:02:27Z","timestamp":1779375747000},"page":"634","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Lipid Remodeling in Mouse SR-B1-Deficient Embryos with Oxidative Stress-Associated Neural Tube Defects"],"prefix":"10.3390","volume":"15","author":[{"given":"Alonso","family":"Quiroz","sequence":"first","affiliation":[{"name":"Ph.D. Program in Medical Science, Faculty of Medicine, Pontificia Universidad Cat\u00f3lica de Chile, Santiago 8331150, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicol\u00e1s","family":"Santander","sequence":"additional","affiliation":[{"name":"Institute of Health Sciences, Universidad de O\u2019Higgins, Rancagua 2820000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4170-5248","authenticated-orcid":false,"given":"Nicholas","family":"Greene","sequence":"additional","affiliation":[{"name":"Great Ormond Street Institute of Child Health, University College London, London WC1N 1EH, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7868-9616","authenticated-orcid":false,"given":"Kit-Yi","family":"Leung","sequence":"additional","affiliation":[{"name":"Great Ormond Street Institute of Child Health, University College London, London WC1N 1EH, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2113-1926","authenticated-orcid":false,"given":"Dolores","family":"Busso","sequence":"additional","affiliation":[{"name":"Biomedical Research and Innovation Center, Faculty of Medicine, Universidad de Los Andes, Santiago 7550000, Chile"},{"name":"Center of Interventional Medicine for Precision and Advanced Cellular Therapy (IMPACT), Santiago 7550000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,5,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1210\/er.2001-0037","article-title":"The role of the high-density lipoprotein receptor SR-BI in the lipid metabolism of endocrine and other tissues","volume":"24","author":"Rigotti","year":"2003","journal-title":"Endocr. Rev."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1016\/j.mam.2007.01.002","article-title":"Absorption, transport, and tissue delivery of vitamin E","volume":"28","author":"Rigotti","year":"2007","journal-title":"Mol. Asp. Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"330","DOI":"10.3945\/an.112.002139","article-title":"Vitamin E","volume":"3","author":"Traber","year":"2012","journal-title":"Adv. Nutr."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1016\/j.freeradbiomed.2007.03.024","article-title":"Vitamin E, antioxidant and nothing more","volume":"43","author":"Traber","year":"2007","journal-title":"Free Radic. Biol. Med."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1732","DOI":"10.1161\/01.ATV.0000091363.28501.84","article-title":"Influence of the HDL receptor SR-BI on lipoprotein metabolism and atherosclerosis","volume":"23","author":"Trigatti","year":"2003","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1093\/jn\/132.3.443","article-title":"Alpha-tocopherol metabolism is abnormal in scavenger receptor class B type I (SR-BI)-deficient mice","volume":"132","author":"Mardones","year":"2002","journal-title":"J. Nutr."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1086","DOI":"10.1093\/hmg\/dds510","article-title":"Developmental abnormalities in mouse embryos lacking the HDL receptor SR-BI","volume":"22","author":"Santander","year":"2013","journal-title":"Hum. Mol. Genet."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Quiroz, A., Belledonne, G., Saavedra, F., Gonz\u00e1lez, J., and Busso, D. (2024). Vitamin E supplementation prevents obesogenic diet-induced developmental abnormalities in SR-B1 deficient embryos. Front. Cell Dev. Biol., 12.","DOI":"10.3389\/fcell.2024.1460697"},{"key":"ref_9","first-page":"87","article-title":"Genetic background influences susceptibility to exencephaly in Scavenger receptor Class B type 1-deficient mouse embryos","volume":"173","author":"Belledonne","year":"2025","journal-title":"Placenta"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1016\/S0022-2275(20)33289-2","article-title":"Temporal and spatial pattern of expression of the HDL receptor SR-BI during murine embryogenesis","volume":"39","author":"Hatzopoulos","year":"1998","journal-title":"J. Lipid Res."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"5182","DOI":"10.1038\/s41598-017-05422-w","article-title":"Deficient Vitamin E Uptake During Development Impairs Neural Tube Closure in Mice Lacking Lipoprotein Receptor SR-BI","volume":"7","author":"Santander","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"7404082","DOI":"10.1155\/2018\/7404082","article-title":"The Role of Oxidative Stress in the Pathomechanism of Congenital Malformations","volume":"2018","author":"Laforgia","year":"2018","journal-title":"Oxidative Med. Cell. Longev."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.freeradbiomed.2014.12.014","article-title":"Markers of macromolecular oxidative damage in maternal serum and risk of neural tube defects in offspring","volume":"80","author":"Yuan","year":"2015","journal-title":"Free Radic. Biol. Med."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1146","DOI":"10.1177\/0300060517734443","article-title":"Amniotic fluid oxidant-antioxidant status in foetal congenital nervous system anomalies","volume":"46","author":"Cim","year":"2018","journal-title":"J. Int. Med. Res."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1080\/15513815.2019.1686783","article-title":"Oxidative-Antioxidative Markers in Pregnant Women with Fetal Neural Tube Defects","volume":"40","author":"Ozel","year":"2021","journal-title":"Fetal Pediatr. Pathol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1016\/j.ajog.2014.11.036","article-title":"Decoding the oxidative stress hypothesis in diabetic embryopathy through proapoptotic kinase signaling","volume":"212","author":"Yang","year":"2015","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"753.e1","DOI":"10.1016\/j.ajog.2020.05.015","article-title":"Deficiency of the oxidative stress-responsive kinase p70S6K1 restores autophagy and ameliorates neural tube defects in diabetic embryopathy","volume":"223","author":"Cao","year":"2020","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1368","DOI":"10.4103\/1673-5374.235249","article-title":"Advanced glycation end products induce neural tube defects through elevating oxidative stress in mice","volume":"13","author":"Li","year":"2018","journal-title":"Neural Regen. Res."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1089\/scd.2017.0203","article-title":"High Glucose Inhibits Neural Stem Cell Differentiation Through Oxidative Stress and Endoplasmic Reticulum Stress","volume":"27","author":"Chen","year":"2018","journal-title":"Stem Cells Dev."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/j.freeradbiomed.2018.01.004","article-title":"Oxidative Stress and Apoptosis in Benzo[a]pyrene-Induced Neural Tube Defects","volume":"116","author":"Lin","year":"2018","journal-title":"Free Radic. Biol. Med."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1016\/j.ijdevneu.2011.06.006","article-title":"Oxidative stress is implicated in arsenic-induced neural tube defects in chick embryos","volume":"29","author":"Han","year":"2011","journal-title":"Int. J. Dev. Neurosci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1086","DOI":"10.1002\/mrd.22865","article-title":"Oxidative stress and cellular and tissue damage in organogenic outbred mouse embryos after moderate perigestational alcohol intake","volume":"84","author":"Coll","year":"2017","journal-title":"Mol. Reprod. Dev."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"979","DOI":"10.1124\/mol.111.072314","article-title":"Valproic acid increases formation of reactive oxygen species and induces apoptosis in postimplantation embryos: A role for oxidative stress in valproic acid-induced neural tube defects","volume":"80","author":"Tung","year":"2011","journal-title":"Mol. Pharmacol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.ijdevneu.2018.04.003","article-title":"Mitochondrial dysfunction is implicated in retinoic acid-induced spina bifida aperta in rat fetuses","volume":"68","author":"Xue","year":"2018","journal-title":"Int. J. Dev. Neurosci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1016\/j.freeradbiomed.2016.04.030","article-title":"Superoxide dismutase 2 overexpression alleviates maternal diabetes-induced neural tube defects, restores mitochondrial function and suppresses cellular stress in diabetic embryopathy","volume":"96","author":"Zhong","year":"2016","journal-title":"Free Radic. Biol. Med."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"5080843","DOI":"10.1155\/2019\/5080843","article-title":"Reactive Oxygen Species-Induced Lipid Peroxidation in Apoptosis, Autophagy, and Ferroptosis","volume":"2019","author":"Su","year":"2019","journal-title":"Oxidative Med. Cell. Longev."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Engel, K.M., Schiller, J., Galuska, C.E., and Fuchs, B. (2021). Phospholipases and Reactive Oxygen Species Derived Lipid Biomarkers in Healthy and Diseased Humans and Animals\u2014A Focus on Lysophosphatidylcholine. Front. Physiol., 12.","DOI":"10.3389\/fphys.2021.732319"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"915","DOI":"10.1007\/BF00403910","article-title":"Diabetes mellitus affects prostaglandin E2 levels in mouse embryos during neurulation","volume":"39","author":"Piddington","year":"1996","journal-title":"Diabetologia"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"496","DOI":"10.1016\/S0891-5849(02)01360-6","article-title":"The selenoprotein GPX4 is essential for mouse development and protects from radiation and oxidative damage insults","volume":"34","author":"Yant","year":"2003","journal-title":"Free Radic. Biol. Med."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.bbalip.2015.10.001","article-title":"The lipoxygenase pathway in zebrafish. Expression and characterization of zebrafish ALOX5 and comparison with its human ortholog","volume":"1861","author":"Adel","year":"2016","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1146\/annurev-nutr-071714-034347","article-title":"Vitamin E: A Role in Signal Transduction","volume":"35","author":"Zingg","year":"2015","journal-title":"Annu. Rev. Nutr."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1113","DOI":"10.1017\/S000711451500272X","article-title":"Vitamin E function and requirements in relation to PUFA","volume":"114","author":"Raederstorff","year":"2015","journal-title":"Br. J. Nutr."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"2855","DOI":"10.1093\/jn\/130.11.2855","article-title":"Low arachidonic acid rather than alpha-tocopherol is responsible for the delayed postnatal development in offspring of rats fed fish oil instead of olive oil during pregnancy and lactation","volume":"130","author":"Amusquivar","year":"2000","journal-title":"J. Nutr."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"478","DOI":"10.1016\/j.jnutbio.2011.02.002","article-title":"Zebrafish (Danio rerio) fed vitamin E-deficient diets produce embryos with increased morphologic abnormalities and mortality","volume":"23","author":"Miller","year":"2012","journal-title":"J. Nutr. Biochem."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.redox.2013.12.007","article-title":"Novel liquid chromatography-mass spectrometry method shows that vitamin E deficiency depletes arachidonic and docosahexaenoic acids in zebrafish (Danio rerio) embryos","volume":"2","author":"Lebold","year":"2013","journal-title":"Redox Biol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1182","DOI":"10.1194\/jlr.M058941","article-title":"Novel function of vitamin E in regulation of zebrafish (Danio rerio) brain lysophospholipids discovered using lipidomics","volume":"56","author":"Choi","year":"2015","journal-title":"J. Lipid Res."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"8227","DOI":"10.1073\/pnas.82.23.8227","article-title":"Hyperglycemia-induced teratogenesis is mediated by a functional deficiency of arachidonic acid","volume":"82","author":"Goldman","year":"1985","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_38","first-page":"179","article-title":"Arachidonic acid reversal of phenytoin-induced neural tube and craniofacial defects in vitro in mice","volume":"8","author":"Kay","year":"1988","journal-title":"J. Craniofacial Genet. Dev. Biol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"818","DOI":"10.1016\/S0002-9378(96)80005-6","article-title":"Dietary polyunsaturated fatty acid prevents malformations in offspring of diabetic rats","volume":"175","author":"Reece","year":"1996","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"790\u2013797; discussion 797\u2013798","DOI":"10.1016\/S0002-9378(97)70602-1","article-title":"Prevention of diabetic embryopathy in offspring of diabetic rats with use of a cocktail of deficient substrates and an antioxidant","volume":"176","author":"Reece","year":"1997","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1007\/s10565-024-09885-2","article-title":"Activation of lipophagy ameliorates cadmium-induced neural tube defects via reducing low density lipoprotein cholesterol levels in mouse placentas","volume":"40","author":"Zhang","year":"2024","journal-title":"Cell Biol. Toxicol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1530\/rep.0.1220131","article-title":"Lipid and fatty acid analysis of fresh and frozen-thawed immature and in vitro matured bovine oocytes","volume":"122","author":"Kim","year":"2001","journal-title":"Reproduction"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"766","DOI":"10.1093\/humrep\/dei385","article-title":"Fatty acid metabolism in human preimplantation embryos","volume":"21","author":"Haggarty","year":"2006","journal-title":"Hum. Reprod."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Zhu, P., Bu, G., Hu, R., Ruan, X., Fu, R., Zhang, Z., Wan, Q., Liu, X., Miao, Y., and Chen, S. (2023). Lipidomic Characterization of Oocytes at Single-Cell Level Using Nanoflow Chromatography-Trapped Ion Mobility Spectrometry-Mass Spectrometry. Molecules, 28.","DOI":"10.3390\/molecules28104202"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1016\/j.theriogenology.2016.03.025","article-title":"Lipidome signatures in early bovine embryo development","volume":"86","author":"Sudano","year":"2016","journal-title":"Theriogenology"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"12610","DOI":"10.1073\/pnas.94.23.12610","article-title":"A targeted mutation in the murine gene encoding the high density lipoprotein (HDL) receptor scavenger receptor class B type I reveals its key role in HDL metabolism","volume":"94","author":"Rigotti","year":"1997","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1540","DOI":"10.1002\/ejlt.201500145","article-title":"An automated shotgun lipidomics platform for high throughput, comprehensive, and quantitative analysis of blood plasma intact lipids","volume":"117","author":"Surma","year":"2015","journal-title":"Eur. J. Lipid Sci. Technol. EJLST"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Herzog, R., Schuhmann, K., Schwudke, D., Sampaio, J.L., Bornstein, S.R., Schroeder, M., and Shevchenko, A. (2012). LipidXplorer: A software for consensual cross-platform lipidomics. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0029851"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1016\/j.jchromb.2006.07.012","article-title":"Quantitative analysis of s-adenosylmethionine and s-adenosylhomocysteine in neurulation-stage mouse embryos by liquid chromatography tandem mass spectrometry","volume":"844","author":"Burren","year":"2006","journal-title":"J. Chromatogr. B"},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Santander, N., Lizama, C., Murgas, L., Contreras, S., Martin, A.J.M., Molina, P., Quiroz, A., Rivera, K., Salas-P\u00e9rez, F., and Godoy, A. (2018). Transcriptional profiling of embryos lacking the lipoprotein receptor SR-B1 reveals a regulatory circuit governing a neurodevelopmental or metabolic decision during neural tube closure. BMC Genom., 19.","DOI":"10.1186\/s12864-018-5110-2"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"2704532","DOI":"10.1155\/2017\/2704532","article-title":"Protective Effect of Icariin on the Development of Preimplantation Mouse Embryos against Hydrogen Peroxide-Induced Oxidative Injury","volume":"2017","author":"Ye","year":"2017","journal-title":"Oxidative Med. Cell. Longev."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1002\/biof.5520080106","article-title":"Specific interaction of methionine adenosyltransferase with free radicals","volume":"8","author":"Avila","year":"1998","journal-title":"BioFactors"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"506","DOI":"10.1002\/bdra.20145","article-title":"A diabetes-like environment increases malformation rate and diminishes prostaglandin E(2) in rat embryos: Reversal by administration of vitamin E and folic acid","volume":"73","author":"Wentzel","year":"2005","journal-title":"Birth Defects Res. Part A Clin. Mol. Teratol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1016\/S0002-9378(86)80001-1","article-title":"Arachidonic acid prevents hyperglycemia-associated yolk sac damage and embryopathy","volume":"155","author":"Pinter","year":"1986","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1139\/o03-073","article-title":"Phospholipid biosynthesis in mammalian cells","volume":"82","author":"Vance","year":"2004","journal-title":"Biochem. Cell Biol. = Biochim. Biol. Cell."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Sun, C., Holstein, D.J.F., Garcia-Cubero, N., Moulla, Y., Stroh, C., Dietrich, A., Sch\u00f6n, M.R., G\u00e4rtner, D., Lohmann, T., and Dressler, M. (2023). The Role of Phosphatidylethanolamine N-Methyltransferase (PEMT) and Its Waist-Hip-Ratio-Associated Locus rs4646404 in Obesity-Related Metabolic Traits and Liver Disease. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms242316850"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1096\/fj.01-0564fje","article-title":"Perturbations in choline metabolism cause neural tube defects in mouse embryos in vitro","volume":"16","author":"Fisher","year":"2002","journal-title":"FASEB J."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1016\/j.plipres.2006.05.003","article-title":"Properties of the Group IV phospholipase A2 family","volume":"45","author":"Ghosh","year":"2006","journal-title":"Prog. Lipid Res."},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Dec, K., Alsaqati, M., Morgan, J., Deshpande, S., Wood, J., Hall, J., and Harwood, A.J. (2023). A high ratio of linoleic acid (n-6 PUFA) to alpha-linolenic acid (n-3 PUFA) adversely affects early stage of human neuronal differentiation and electrophysiological activity of glutamatergic neurons in vitro. Front. Cell Dev. Biol., 11.","DOI":"10.3389\/fcell.2023.1166808"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1016\/j.neulet.2017.05.020","article-title":"The ratio of 1\/3 linoleic acid to alpha linolenic acid is optimal for oligodendrogenesis of embryonic neural stem cells","volume":"651","author":"Hejr","year":"2017","journal-title":"Neurosci. Lett."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.brainres.2008.08.078","article-title":"Dietary omega 3 fatty acids and the developing brain","volume":"1237","author":"Innis","year":"2008","journal-title":"Brain Res."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1002\/bdrb.20311","article-title":"Essential fatty acid supplementation of DHA and ARA and effects on neurodevelopment across animal species: A review of the literature","volume":"92","author":"Tassinari","year":"2011","journal-title":"Birth Defects Res. Part B Dev. Reprod. Toxicol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/j.redox.2016.01.004","article-title":"Lipidomics and H218O labeling techniques reveal increased remodeling of DHA-containing membrane phospholipids associated with abnormal locomotor responses in \u03b1-tocopherol deficient zebrafish (Danio rerio) embryos","volume":"8","author":"McDougall","year":"2016","journal-title":"Redox Biol."},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Naud\u00ed, A., Jov\u00e9, M., Ayala, V., Portero-Ot\u00edn, M., Barja, G., and Pamplona, R. (2013). Membrane lipid unsaturation as physiological adaptation to animal longevity. Front. Physiol., 4.","DOI":"10.3389\/fphys.2013.00372"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1016\/j.chembiol.2018.11.016","article-title":"Exogenous Monounsaturated Fatty Acids Promote a Ferroptosis-Resistant Cell State","volume":"26","author":"Magtanong","year":"2019","journal-title":"Cell Chem. Biol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1016\/j.chembiol.2023.10.012","article-title":"Ferroptosis inhibition by oleic acid mitigates iron-overload-induced injury","volume":"31","author":"Mann","year":"2024","journal-title":"Cell Chem. Biol."},{"key":"ref_67","first-page":"435","article-title":"Lipid Droplets and the Management of Cellular Stress","volume":"92","author":"Jarc","year":"2019","journal-title":"Yale J. Biol. Med."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"1260","DOI":"10.1016\/j.bbalip.2017.07.006","article-title":"Lipid droplet functions beyond energy storage","volume":"1862","author":"Welte","year":"2017","journal-title":"Biochim. Biophys. Acta\u2014Mol. Cell Biol. Lipids"},{"key":"ref_69","doi-asserted-by":"crossref","unstructured":"Petan, T., Jarc, E., and Jusovi\u0107, M. (2018). Lipid Droplets in Cancer: Guardians of Fat in a Stressful World. Molecules, 23.","DOI":"10.3390\/molecules23081941"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1038\/s41580-018-0085-z","article-title":"Dynamics and functions of lipid droplets","volume":"20","author":"Olzmann","year":"2019","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1016\/j.cell.2015.09.020","article-title":"Antioxidant Role for Lipid Droplets in a Stem Cell Niche of Drosophila","volume":"163","author":"Bailey","year":"2015","journal-title":"Cell"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/tera.1420510102","article-title":"Exencephaly and hydrocephaly in mice with targeted modification of the apolipoprotein B (Apob) gene","volume":"51","author":"Homanics","year":"1995","journal-title":"Teratology"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"8460","DOI":"10.1073\/pnas.93.16.8460","article-title":"Defective forebrain development in mice lacking gp330\/megalin","volume":"93","author":"Willnow","year":"1996","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_74","unstructured":"(2011). Guide for the Care and Use of Laboratory Animals, National Academy of Sciences."}],"container-title":["Antioxidants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-3921\/15\/5\/634\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T10:53:48Z","timestamp":1781261628000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-3921\/15\/5\/634"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,16]]},"references-count":74,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2026,5]]}},"alternative-id":["antiox15050634"],"URL":"https:\/\/doi.org\/10.3390\/antiox15050634","relation":{},"ISSN":["2076-3921"],"issn-type":[{"value":"2076-3921","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,16]]}}}