{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T14:55:36Z","timestamp":1787237736257,"version":"build-2736575974"},"reference-count":89,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T00:00:00Z","timestamp":1780444800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>Flavonoids from Pinus koraiensis needle (PN) litterfall were efficiently recovered using an enzyme-assisted ultrasonic extraction (EAU) method optimized via response surface methodology (RSM). The optimal conditions (enzyme dosage 1.7%, ethanol concentration 70%, ultrasonic time 21 min, cellulase\u2013pectinase ratio 1:3, liquid\u2013solid ratio 40:1, enzymatic hydrolysis at 42.5 \u00b0C for 1 h, ultrasonic extraction at 50 \u00b0C and 150 W) yielded a total flavonoid content (TFC) of 17.08 mg rutin\/g, which was significantly higher than that obtained via conventional extraction (CE). Scanning electron microscopy (SEM) confirmed that the treatment disrupted the cell wall, promoting flavonoid release. Ultra-performance liquid chromatography coupled with triple-quadrupole time-of-flight mass spectrometry (UPLC-Triple-TOF\/MS) identified 60 flavonoids in the purified extract obtained under the optimal EAU conditions (OT group), including quercitrin, tiliroside, taxifolin, and procyanidin B2. Fourier-transform infrared spectroscopy (FTIR) and differential scanning calorimetry (DSC) showed higher crystallinity but slightly reduced thermal stability for OT flavonoids. Notably, compared with the purified flavonoids obtained by CE (CK1 group), the OT group achieved a higher TFC and exhibited significantly better in vitro antioxidant activity (DPPH IC50 = 71.82 \u03bcg\/mL; ABTS IC50 = 28.93 \u03bcg\/mL) and in vitro carbohydrate-digesting-enzyme-inhibitory activity (\u03b1-glucosidase (\u03b1-GLU) IC50 = 79.52 \u03bcg\/mL; \u03b1-amylase (\u03b1-AMY) IC50 = 793.9 \u03bcg\/mL), with \u03b1-AMY inhibition being approximately 8.2-fold higher. These findings suggest that enzyme-assisted ultrasonic extraction is an efficient and reliable method for recovering flavonoids from PN and may provide a theoretical reference for the development and utilization of these flavonoids.<\/jats:p>","DOI":"10.3390\/antiox15060712","type":"journal-article","created":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T18:08:07Z","timestamp":1780510087000},"page":"712","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Enzyme-Assisted Ultrasonic Extraction of Flavonoids from Pinus koraiensis Needle Litterfall: Process Optimization, Component Identification, and In Vitro Bioactivity Evaluation"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1249-8769","authenticated-orcid":false,"given":"Weiwei","family":"Liang","sequence":"first","affiliation":[{"name":"College of Food Science, Northeast Agricultural University, Harbin 150030, China"},{"name":"College of Food Engineering, Harbin University, Harbin 150086, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Le","family":"Ouyang","sequence":"additional","affiliation":[{"name":"College of Food Engineering, Harbin University, Harbin 150086, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun","family":"Bian","sequence":"additional","affiliation":[{"name":"College of Food Engineering, Harbin University, Harbin 150086, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuxin","family":"Shan","sequence":"additional","affiliation":[{"name":"College of Food Engineering, Harbin University, Harbin 150086, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiufang","family":"Xia","sequence":"additional","affiliation":[{"name":"College of Food Science, Northeast Agricultural University, Harbin 150030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Shamsudin, N.F., Ahmed, Q.U., Mahmood, S., Ali Shah, S.A., Khatib, A., Mukhtar, S., Alsharif, M.A., Parveen, H., and Zakaria, Z.A. 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