{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T04:07:07Z","timestamp":1790136427313,"version":"4.0.1"},"reference-count":46,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T00:00:00Z","timestamp":1781481600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"Ministry of Science, ICT and Future Planning","doi-asserted-by":"publisher","award":["NRF-RS-2024-00336513"],"award-info":[{"award-number":["NRF-RS-2024-00336513"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"award":["NRF-RS-2024-00336513"],"award-info":[{"award-number":["NRF-RS-2024-00336513"]}],"id":[{"id":"https:\/\/ror.org\/023ktd084","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>Allomyrinasin is an antimicrobial peptide derived from the larvae of the edible insect Allomyrina dichotoma and has been reported to exert anti-inflammatory activity, although its role in metabolic regulation remains unclear. This study aimed to investigate the metabolic and hepatoprotective effects of allomyrinasin in a high-fat diet (HFD)\u2013induced obese mouse model. Male C57BL\/6J mice were fed an HFD for 6 weeks to induce body weight gain and hyperglycemia, followed by 10 weeks of oral administration of allomyrinasin (0.1 mg\/kg\/day) under continued HFD conditions, with metformin used as a positive control. Metabolic parameters related to glucose homeostasis, insulin sensitivity, lipid metabolism, hepatic injury, oxidative stress, inflammation, and fibrotic responses were evaluated. Allomyrinasin significantly attenuated HFD-associated body weight gain and improved glucose tolerance and insulin sensitivity. These effects were accompanied by favorable modulation of serum lipid profiles and suppression of hepatic lipogenic signaling, including reduced expression of key regulators of de novo lipogenesis. In parallel, allomyrinasin mitigated hepatic inflammatory, fibrotic, and oxidative stress-related alterations, as reflected by improvements in biochemical markers and molecular analyses. Collectively, these findings indicate that allomyrinasin contributes to the improvement of metabolic regulation and hepatic homeostasis in HFD-fed mice. Our results support allomyrinasin as a promising food-applicable bioactive peptide and potential functional ingredient for the prevention or management of obesity-related metabolic disorders.<\/jats:p>","DOI":"10.3390\/antiox15060755","type":"journal-article","created":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T08:22:56Z","timestamp":1781684576000},"page":"755","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Allomyrinasin, an Edible Insect-Derived Peptide, Ameliorates High-Fat Diet-Induced Hepatic Oxidative Stress and Metabolic Dysfunction"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8354-0488","authenticated-orcid":false,"given":"Kyong","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Food and Nutrition, Eulji University, Seongnam 13135, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chae-Heon","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Food and Nutrition, Eulji University, Seongnam 13135, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chae-Eun","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Food and Nutrition, Eulji University, Seongnam 13135, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eun-Young","family":"Park","sequence":"additional","affiliation":[{"name":"College of Pharmacy and Natural Medicine Research Institute, Mokpo National University, Jeoannam, Muan-gun 58554, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jae-Sam","family":"Hwang","sequence":"additional","affiliation":[{"name":"Future Food Research Institute, Hanmi Nutrition, Paju 10808, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3995-4429","authenticated-orcid":false,"given":"Yoon Sin","family":"Oh","sequence":"additional","affiliation":[{"name":"Department of Food and Nutrition, Eulji University, Seongnam 13135, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1172\/JCI92035","article-title":"Inflammatory mechanisms linking obesity and metabolic disease","volume":"127","author":"Saltiel","year":"2017","journal-title":"J. Clin. Investig."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1002\/hep.28431","article-title":"Global epidemiology of nonalcoholic fatty liver disease\u2014Meta-analytic assessment of prevalence, incidence, and outcomes","volume":"64","author":"Younossi","year":"2016","journal-title":"Hepatology"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"908","DOI":"10.1038\/s41591-018-0104-9","article-title":"Mechanisms of NAFLD development and therapeutic strategies","volume":"24","author":"Friedman","year":"2018","journal-title":"Nat. Med."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"e24045","DOI":"10.1016\/j.heliyon.2024.e24045","article-title":"Bioactive compounds and biological activity in edible insects: A review","volume":"10","year":"2024","journal-title":"Heliyon"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"139","DOI":"10.3920\/JIFF2016.0050","article-title":"If termiteCoptotermes acinaciformis is not an intermediate host, could it be a safe food source for humans?","volume":"3","author":"King","year":"2017","journal-title":"J. Insects Food Feed"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"112094","DOI":"10.1016\/j.jtbi.2025.112094","article-title":"The spatial aggregation of phytophagous insects driven by the evolution of preference for plant chemicals","volume":"605","author":"Ohsaki","year":"2025","journal-title":"J. Theor. Biol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1111\/eea.13241","article-title":"An omics evolutionary perspective on phytophagous insect\u2013host plant interactions in Anastrepha obliqua: A review","volume":"171","year":"2023","journal-title":"Entomol. Exp. Appl."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"5807","DOI":"10.1007\/s00253-014-5792-6","article-title":"Insect antimicrobial peptides and their applications","volume":"98","author":"Yi","year":"2014","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1038\/s41579-025-01200-y","article-title":"Antimicrobial peptides: Structure, functions and translational applications","volume":"23","author":"Souza","year":"2025","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/j.peptides.2015.05.012","article-title":"Antimicrobial peptides: Possible anti-infective agents","volume":"72","author":"Chen","year":"2015","journal-title":"Peptides"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1690","DOI":"10.1080\/10408398.2025.2553674","article-title":"Exploring the bioactive potential of edible insects for a sustainable future","volume":"66","author":"Lee","year":"2026","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Zhou, L., Meng, G., Zhu, L., Ma, L., and Chen, K. (2024). Insect Antimicrobial Peptides as Guardians of Immunity and Beyond: A Review. Int. J. Mol. Sci., 25.","DOI":"10.20944\/preprints202403.0123.v1"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Kim, K., Bae, G.D., Lee, M., Park, E.-Y., Baek, D.J., Kim, C.Y., Jun, H.-S., and Oh, Y.S. (2019). Allomyrina dichotoma Larva Extract Ameliorates the Hepatic Insulin Resistance of High-Fat Diet-Induced Diabetic Mice. Nutrients, 11.","DOI":"10.3390\/nu11071522"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"687","DOI":"10.4014\/jmb.1809.09031","article-title":"Anti-Inflammatory Activity of Antimicrobial Peptide Allomyrinasin Derived from the Dynastid Beetle, Allomyrina dichotoma","volume":"29","author":"Lee","year":"2019","journal-title":"J. Microbiol. Biotechnol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1038\/nature21363","article-title":"Inflammation, metaflammation and immunometabolic disorders","volume":"542","author":"Hotamisligil","year":"2017","journal-title":"Nature"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"860","DOI":"10.1038\/nature05485","article-title":"Inflammation and metabolic disorders","volume":"444","author":"Hotamisligil","year":"2006","journal-title":"Nature"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1038\/nri2925","article-title":"Type 2 diabetes as an inflammatory disease","volume":"11","author":"Donath","year":"2011","journal-title":"Nat. Rev. Immunol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1016\/j.lfs.2015.10.025","article-title":"Men and mice: Relating their ages","volume":"152","author":"Sengupta","year":"2016","journal-title":"Life Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1038\/s41538-019-0050-z","article-title":"Pyroglutamyl leucine, a peptide in fermented foods, attenuates dysbiosis by increasing host antimicrobial peptide","volume":"3","author":"Shirako","year":"2019","journal-title":"npj Sci. Food"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"3979352","DOI":"10.1155\/2019\/3979352","article-title":"Enhancement of Macrophage Function by the Antimicrobial Peptide Sublancin Protects Mice from Methicillin-Resistant Staphylococcus aureus","volume":"2019","author":"Wang","year":"2019","journal-title":"J. Immunol. Res."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1007\/BF00280883","article-title":"Homeostasis model assessment: Insulin resistance and \u03b2-cell function from fasting plasma glucose and insulin concentrations in man","volume":"28","author":"Matthews","year":"1985","journal-title":"Diabetologia"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Oates, J.R., McKell, M.C., Moreno-Fernandez, M.E., Damen, M.S.M.A., Deepe, G.S., Qualls, J.E., and Divanovic, S. (2019). Macrophage Function in the Pathogenesis of Non-alcoholic Fatty Liver Disease: The Mac Attack. Front. Immunol., 10.","DOI":"10.3389\/fimmu.2019.02893"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1007\/s13679-023-00519-y","article-title":"The Role of Tumor Necrosis Factor-Alpha in the Pathogenesis and Treatment of Nonalcoholic Fatty Liver Disease","volume":"12","author":"Vachliotis","year":"2023","journal-title":"Curr. Obes. Rep."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1016\/j.tcm.2015.10.004","article-title":"Metabolic syndrome update","volume":"26","author":"Grundy","year":"2016","journal-title":"Trends Cardiovasc. Med."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1071","DOI":"10.1007\/s00125-015-3531-x","article-title":"In adipose tissue, increased mitochondrial emission of reactive oxygen species is important for short-term high-fat diet-induced insulin resistance in mice","volume":"58","author":"Paglialunga","year":"2015","journal-title":"Diabetologia"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2474","DOI":"10.2337\/db11-0194","article-title":"Inflammation is necessary for long-term but not short-term high-fat diet-induced insulin resistance","volume":"60","author":"Lee","year":"2011","journal-title":"Diabetes"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"173","DOI":"10.2337\/diacare.14.3.173","article-title":"Insulin Resistance: A Multifaceted Syndrome Responsible for NIDDM, Obesity, Hypertension, Dyslipidemia, and Atherosclerotic Cardiovascular Disease","volume":"14","author":"DeFronzo","year":"1991","journal-title":"Diabetes Care"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1016\/j.tem.2010.01.009","article-title":"Gluttony, sloth and the metabolic syndrome: A roadmap to lipotoxicity","volume":"21","author":"Unger","year":"2010","journal-title":"Trends Endocrinol. Metab."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"710","DOI":"10.1038\/nrendo.2017.91","article-title":"SREBP-regulated lipid metabolism: Convergent physiology\u2014divergent pathophysiology","volume":"13","author":"Shimano","year":"2017","journal-title":"Nat. Rev. Endocrinol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1836","DOI":"10.1002\/hep.24001","article-title":"Evolution of inflammation in nonalcoholic fatty liver disease: The multiple parallel hits hypothesis","volume":"52","author":"Tilg","year":"2010","journal-title":"Hepatology"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1172\/JCI24282","article-title":"Liver fibrosis","volume":"115","author":"Bataller","year":"2005","journal-title":"J. Clin. Investig."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Fabregat, I., and Caballero-D\u00edaz, D. (2018). Transforming Growth Factor-\u03b2-Induced Cell Plasticity in Liver Fibrosis and Hepatocarcinogenesis. Front. Oncol., 8.","DOI":"10.3389\/fonc.2018.00357"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Dewidar, B., Meyer, C., Dooley, S., and Meindl-Beinker, a.N. (2019). TGF-\u03b2 in Hepatic Stellate Cell Activation and Liver Fibrogenesis\u2014Updated 2019. Cells, 8.","DOI":"10.3390\/cells8111419"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Zhang, Y., Ren, L., Tian, Y., Guo, X., Wei, F., and Zhang, Y. (2024). Signaling pathways that activate hepatic stellate cells during liver fibrosis. Front Med., 11.","DOI":"10.3389\/fmed.2024.1454980"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.freeradbiomed.2011.10.003","article-title":"Role of oxidative stress in the pathogenesis of nonalcoholic steatohepatitis","volume":"52","author":"Rolo","year":"2012","journal-title":"Free Radic. Biol. Med."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1016\/j.freeradbiomed.2007.11.004","article-title":"CYP2E1 and oxidative liver injury by alcohol","volume":"44","author":"Lu","year":"2008","journal-title":"Free Radic. Biol. Med."},{"key":"ref_37","first-page":"657","article-title":"CYP2E1--biochemical and toxicological aspects and role in alcohol-induced liver injury","volume":"73","author":"Cederbaum","year":"2006","journal-title":"Mt. Sinai J. Med."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/0005-2760(82)90150-3","article-title":"Purification from pig liver of a protein which protects liposomes and biomembranes from peroxidative degradation and exhibits glutathione peroxidase activity on phosphatidylcholine hydroperoxides","volume":"710","author":"Ursini","year":"1982","journal-title":"Biochim. Biophys. Acta BBA\u2014Lipids Lipid Metab."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"3289","DOI":"10.1016\/j.bbagen.2012.11.020","article-title":"Glutathione peroxidases","volume":"1830","author":"Maiorino","year":"2013","journal-title":"Biochim. Biophys. Acta BBA\u2014General. Subj."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/0891-5849(91)90192-6","article-title":"Chemistry and biochemistry of 4-hydroxynonenal, malonaldehyde and related aldehydes","volume":"11","author":"Esterbauer","year":"1991","journal-title":"Free Radic. Biol. Med."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/j.tcb.2015.10.014","article-title":"Ferroptosis: Death by Lipid Peroxidation","volume":"26","author":"Yang","year":"2016","journal-title":"Trends Cell Biol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1016\/j.numecd.2005.05.003","article-title":"A review of recent studies on malondialdehyde as toxic molecule and biological marker of oxidative stress","volume":"15","author":"Stewart","year":"2005","journal-title":"Nutr. Metab. Cardiovasc. Dis."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/S0168-8278(02)00073-9","article-title":"In situ detection of lipid peroxidation and oxidative DNA damage in non-alcoholic fatty liver diseases","volume":"37","author":"Seki","year":"2002","journal-title":"J. Hepatol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1016\/j.jhep.2006.07.010","article-title":"The importance of platelet-derived serotonin in mediating hepatic regeneration","volume":"45","author":"Jeyabalan","year":"2006","journal-title":"J. Hepatol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1494","DOI":"10.1172\/JCI26498","article-title":"MCP-1 contributes to macrophage infiltration into adipose tissue, insulin resistance, and hepatic steatosis in obesity","volume":"116","author":"Kanda","year":"2006","journal-title":"J. Clin. Investig."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"826","DOI":"10.1002\/hep.21108","article-title":"COX-2 induction in mice with experimental nutritional steatohepatitis: Role as pro-inflammatory mediator","volume":"43","author":"Yu","year":"2006","journal-title":"Hepatology"}],"container-title":["Antioxidants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-3921\/15\/6\/755\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T09:11:53Z","timestamp":1781687513000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-3921\/15\/6\/755"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,15]]},"references-count":46,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2026,6]]}},"alternative-id":["antiox15060755"],"URL":"https:\/\/doi.org\/10.3390\/antiox15060755","relation":{},"ISSN":["2076-3921"],"issn-type":[{"value":"2076-3921","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,15]]}}}